The document describes the validation of a real-time PCR assay for detecting Ureaplasma urealyticum (UU) developed by researchers at Indiana University School of Medicine. They optimized a previously published RT-PCR assay to amplify a 152 base pair fragment of UU. This fragment was cloned and purified to generate quantitative standards. Using these standards, the researchers optimized reaction conditions and determined the assay could detect down to approximately 10 copies/microliter of UU. Specificity testing found the assay correctly identified UU and did not detect Ureaplasma parvum. The validated assay will be used to determine the prevalence of UU in patients attending a local STI clinic and establish the limit of