Download to read offline
FDR Presentation
- 1. COST EFFECTIVE TEST TUBEHOLDER FOR THE FACS ARIA III CAPSTONE GROUP #13: STEM CELL INSTRUMENTATION FOUNDRY (SCIF) PROJECT 1
- 2. MEET THE TEAM ChrisCruz Team Lead Bioengineering Ryan Burtzlaff Chief Financial Officer Bioengineering Diane Martinez Safety Officer Bioengineering Socorro Martinez Test Engineer Bioengineering 2
- 3. MISSION STATEMENT To streamlinebiological cell sorting by creating a cost-effective custom designed device to improve both product collection and cell viability 3
- 4. SPONSOR: DR. DAVIDGRAVANO •Stem Cell Instrumentation Facility (SCIF) Lead •Project Scientist; Flow Cytometry and cell Sorting •Provides an array of services 4
- 5. STEM CELLS • Unspecifiedcells capable of self- renewing and producing specialized cells through differentiation • The capability of differentiating into one of the 220 different cells in the body • Research on stem cells is very important for Tissue Engineering and Drug testing • Stem Cells need to be first sorted due to cell population purity Sharkis et al., Sci Transl Med 2012 5
- 6. BD FACS ARIAIII CELL SORTER 6
- 7. FLOW CYTOMETRY • Measurescells or particles in liquid suspension • Collects quantitative and qualitative data • BD Flow Cytometry Aria Cell Sorter (FACS) • Allows for up to 4-5 streams of separation • Runs for hours at a time, sorting millions of cells 7
- 8. Challenges • Problem 1 •BD Bioscience Test Tube Holders are manufactured to only hold up to 3 collection sizes. • Problem 2 • Cell Sorter is insufficient with mixing the sheath fluid and the serum fluid together during the sorting process thus decreasing viability. Solutions Sheath Fluid Serum/Cell Media • Solution 1 • Create a cost-effective test tube holder than can hold more than 3 collection sizes. • Solution 2 • Implement a magnetic stirring plate onto the Test Tube Holder to suspend both fluids. 8
- 9. CHALLENGE 1: DEVELOPA VARIABLE SIZE TEST TUBE HOLDER • Test Tube Holders • 2x50mL • 1x50mL, 2x5mL • 2x15mL, 2x5mL 9
- 10. CELL SORTING ANDFITTING TEST 10
- 11. OBJECTIVE 2: DEVELOPA MIXING MECHANISM 11
- 12. FINANCIAL Item Cost (Dollars) TestTube Holder 3x2 469.60 Magnetic Motor Parts 33 Total: 571.13 • BDBiosciences Device: >$400/unit • Does not include mixing capabilities • Does not have uneven tube option • Estimated cost for 6 devices: $2400 Overall Savings = $1897.38 For any university or research facility with access to CAD files and 3D printing Overall Savings = $1897.38 For any university or research facility with access to CAD files and 3D printing 12
- 13. ACCOMPLISHMENTS By creating aCost-Effective Varied Test Tube Size Holder that also includes internal mixing capability we were able to improve cell sorting collection and potentially increase cell viability. These designs are distributable and repeatable enough for any cell sorting facility to benefit from them. 13
- 14.
Editor's Notes
- #3 Images stretched
- #4 Punctiatoin bring back mission statement in conclusion, switch cell viability and product collection
- #5 Concentrating on flow cytometry
- #6 Introduction is too long
- #9 Increase fonts, Title: Challenges, can only hold 3 collections
- #10 Take out objective 1, say something else. Talk about ALL three prototypes and make better images. Don’t say final design. Fuck it delete all the texts. Test this and this, had this be the best. In addition, it has to be both water tight and transparent to accommodate to cooling mechanism and let researchers see how full the tubes are so we used this material and made it transparent.
- #12 Second goal is improving cell viability by mixing the fluids inside the vessel. Here we had two different ideas. Take out the pro’s and cons and just include the pictures
- #13 We solved this problem and this is the impact. Add commercially price to the problem section.
- #14 Improve the efficiency by making these sizes and were able to conclude that it was able to collect this and were able to improve cell viability by adding these magnetic stir plates. SAY POTENTIALLY.