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Waxy layer which protects the
plant & conserves water
mesophyll
Contains
chloroplasts:
Specialized for
light absorption
Layer contains air
spaces: Specialized
for gas exchange
Exchange water &
gases with the
atmosphere
Vascular
Tissue
RecapRecap
What you will learnWhat you will learn
(Transport Structures)(Transport Structures)
Xylem vessels and phloem (Vascular
Tissue)
◦ Know their positions and functions within a
dicotyledonous leaf and stem, under light
microscope
Root
◦ Understand its structure (root hairs) and
functions
◦ Facilitate water & ion (minerals) uptake
What you will learnWhat you will learn
(Transport of water)(Transport of water)
Briefly explain the movement of water from
the roots to the leaves
◦ Through Xylem Vessels
Define the term ‘transpiration’
◦ Understand that transpiration pull helps to
move the water upwards; from the roots to
the leaves
◦ Create a water potential gradient
4 Factors affecting transpiration rate
What you will learnWhat you will learn
(Transport of Food)(Transport of Food)
Translocation (Transport of food)
◦ Through Phloem tissue
Briefly illustrate translocation through
translocation studies
◦ Using aphids
◦ The Ringing Experiment
◦ Using Radio-isotopes
Wilting
◦ Advantages and Disadvantages
Recall on PhotosynthesisRecall on Photosynthesis
How does
food get
from the
leaves to
other parts
of the plant?
How does
water get
absorb from
the soil into
and move
around the
plant?
Transport StructuresTransport Structures
XylemXylem
Structures
1.Dead tissue
2.Long and empty lumen
3.Walls are thicken strengthen
with lignin
Long hollow tube extending from the root to the
leaves
Development of XylemDevelopment of Xylem
Tracheids Xylem vessels
Xylem tissue contains
Xylem cells (Tracheids) and Xylem vessels
Tracheids (Xylem Cells)
Long, tapered cells with pitted walls that
transport materials upwards
At maturity,
lignin can be seen deposited at the cell walls
and the tracheids gradually dies
no cytoplasm
Xylem Vessels
Long continuous, nonliving hollow tube
No cytoplasm
Walls are strengthened by lignin
LigninLignin
Hard and rigid substance
Named after the latin word “Lignum”, which
means “Wood”
Lignin maybe deposited in different ways,
giving rise to annular, spiral or pitted xylem
vessels
XylemXylem
2 Functions
a)Conducts water and dissolved minerals from the
roots to all other parts of the plant
b)Provides mechanical support to the plant due to the
presence of lignin
2 Adaptations
a)Having a continuous lumen without any protoplasm
within to hinder transport of water & dissolved minerals
b)Having lignified walls which prevent collapse of the
vessels
Do you know?Do you know?
PhloemPhloem
Structures
Consists of a column of
sieve tubes and companion
cells
Sieve tubes are separated by
sieve plates
Sieve TubesSieve Tubes
Degenerate Protoplasm
No vacuole
No nucleus
Presence of cytoplasm
Cytoplasm remains so that
concentration gradient of
manufactured food can be
maintained
Companion CellsCompanion Cells
Narrow, thin wall with
abundance of cytoplasm
and a nucleus
Functions
a)Keep the sieve tube alive
 Provide nutrients
a)Contains many
mitochondria
 Provide energy for
translocation
sieve plate
sieve
tube cell
companion cell
companion
cell
sieve plate
Organization of vascular tissue inOrganization of vascular tissue in
StemStem
1. Vascular Bundle
The xylem and phloem are
grouped together to form
the vascular bundles
Organization of vascular tissue inOrganization of vascular tissue in
StemStem
1. Vascular Bundle
2. Cambium
Cambium cells can divide
to give rise to new xylem
and phloem tissues, hence
thickening of the stem
Phloem
Cambium
Xylem
Organization of vascular tissue inOrganization of vascular tissue in
StemStem
1. Vascular Bundle
2. Cambium
The vascular bundles are
arranged in a ring
surrounding a central
region called pith which
serves as a storage tissue
for food substances
3. Pith
Organization of vascular tissue inOrganization of vascular tissue in
StemStem
1. Vascular Bundle
2. Cambium
The region between the
vascular bundles and
epidermis is the cortex,
which also serves as a
storage tissue for food
substances
3. Pith
3. Cortex
Organization of vascular tissue inOrganization of vascular tissue in
StemStem
1. Vascular Bundle
2. Cambium
The epidermal cells are
covered with a layer of
wax called cuticle which
prevents excessive loss of
water from the stem
3. Pith
4. Cortex
5. Epidermis
Organization of vascular tissue inOrganization of vascular tissue in
StemStem
1. Vascular Bundle
2. Cambium
3. Pith
4. Cortex
5. Epidermis
Phloem
Cambium
Xylem
Organization of vascular tissue inOrganization of vascular tissue in RootRoot
1. Vascular Tissue xylem
phloem
The xylem and phloem
are NOT bundle
together. They alternate
with each other
Organization of vascular tissue inOrganization of vascular tissue in RootRoot
1. Vascular Tissue
The region between
the epidermis and the
endodermis lies the
Cortex, which serves
as storage tissue
2. Cortex
Endodermis
Organization of vascular tissue inOrganization of vascular tissue in RootRoot
1. Vascular Tissue
The epidermis of the
root bearing the root
hairs are called the
piliferous layer.
NO cuticle is present!
Why?
2. Cortex
3. Piliferous layer
Organization of vascular tissue inOrganization of vascular tissue in RootRoot
1. Vascular Tissue
Each root hair is a
tubular outgrowth of
an epidermal cell.
2. Cortex
3. Piliferous layer
4. Root hair
Organization of vascular tissue inOrganization of vascular tissue in RootRoot
1. Vascular Tissue
2. Cortex
3. Piliferous layer
4. Root hair
xylem
phloem
What are the
functions and
adaptations of Root/
Root hairs?
Functions
1.Anchor the plant
2.Specialized in absorption of water &
dissolved minerals
Adaptations
1.Long and narrow
 Increase surface area to volume ratio
2.Cells are alive
 Provide energy for active transport
3.Root hairs have cell sap of higher concentration than surrounding soil
solution
 Cell sap contains sugar, mineral salts which helps to prevent
water leaking out of cell. Assist in osmosis
Transport StructuresTransport Structures
Leaf Stem Root
Xylem
Xylem
Phloem
Xylem
Phloem
Phloem
Transport of WaterTransport of Water
Root
Stem
Leaves
Water uptake in ROOTWater uptake in ROOT Animation
Root PressureRoot Pressure
Osmotic pressure that build up within the root cells which
forces water up the root xylem
Recap on Osmosis
How does the root
maintain a
concentration
gradient?
Root PressureRoot Pressure
 Uptake of water by the root hair by Osmosis
 Uptake of dissolved minerals through active
transport
 Cell sap within the root hairs becomes more
concentrated than the water in the soil
Root PressureRoot Pressure
Occurs when the soil moisture level is
high either at night or when transpiration
is low during the day
Can only raise the water in some plants
up to 20m
Not the main force
Water transport in STEMWater transport in STEM
You have learnt that water
is transported from the
root to the stem and the
leaf.
How does the plant
transport water
upwards against
gravity??
TranspirationTranspiration
 Evaporation of water from the plant
 Loss of water vapor through the stomata on the
underside of the leaves
1. The mesophyll cells in the leaves are covered with a
thin layer of moisture
 Essential for efficient gas exchange to occur
1. Some of this moisture evaporates into the
intercellular spaces which diffuses through the
stomata into the ‘drier’ air
 Water is pulled upwards through osmosis
(Transpiration Pull)
1. The waterway in which the water moves from a
higher water potential to a lower water potential
 Transpiration Stream
4 Environmental Factors that affect4 Environmental Factors that affect
TranspirationTranspiration
1. Wind speed
2. Humidity
3. Light intensity
4. Temperature
5. Water supply
Animation
Light intensityLight intensity
During the day, stomata
of the leaves open. Why?
Photosynthesis!!
Gases exchange (CO2 & O2)
Water vapor also evaporates
(Transpiration)
TemperatureTemperature
The higher the temperature, the higher
the air water capacity to hold moisture
At 30ºC, a leaf
may transpire 3
times as fast as
it does at 20ºC
5 Factors that affect Transpiration5 Factors that affect Transpiration
1. Wind speed
◦ Increases  Increases transpiration rate
1. Humidity
◦ Increases  Decreases transpiration rate
1. Light intensity
◦ Increases  Increases transpiration rate
1. Temperature
◦ Increases  Increases transpiration rate
1. Water supply
◦ Decreases  Decreases transpiration
rate
PotometerPotometer
The rate at which plants take up water depends on the
rate of transpiration- the faster a plant transpires, the
faster it takes up water.
Capillary ActionCapillary Action
The attractive force between the molecules of a
particular liquid is known as Cohesion
Water coheres to each other via chemical bonds called
hydrogen bonds (holds the droplets of water together)
• The attractive force between two unlike materials
is known as Adhesion
Adhesion causes water to stick to the inside of the
glass
When water passes up the thin xylem vessels, it
adheres to the surface of the vessels, while the
force of osmosis gently ‘pushes’ the water
molecules, which cohere to each other, upwards
Capillary ActionCapillary Action
Forces that promotes uptake ofForces that promotes uptake of
waterwater
1. Root Pressure
 Promotes uptake of water in the root
1. Transpiration pull
 Main force to ‘suck’ up the water
1. Capillary Action
 Pushes the water upwards due to the
adhesion between the walls of the xylem
vessels and water molecules as well as the
cohesion between the water molecules
Transport of FoodTransport of Food
Transport of Food in plantTransport of Food in plant
Food substances (sugar & amino acids) are
manufactured in the green leaves through the
process called photosynthesis
Translocation is the process of transporting food
substances downwards from the leaves to all other
parts of the plant, through the phloem
Animation
Animation

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Leaf Structure and Transport in Plants

  • 1.
  • 2. Waxy layer which protects the plant & conserves water mesophyll Contains chloroplasts: Specialized for light absorption Layer contains air spaces: Specialized for gas exchange Exchange water & gases with the atmosphere Vascular Tissue RecapRecap
  • 3. What you will learnWhat you will learn (Transport Structures)(Transport Structures) Xylem vessels and phloem (Vascular Tissue) ◦ Know their positions and functions within a dicotyledonous leaf and stem, under light microscope Root ◦ Understand its structure (root hairs) and functions ◦ Facilitate water & ion (minerals) uptake
  • 4. What you will learnWhat you will learn (Transport of water)(Transport of water) Briefly explain the movement of water from the roots to the leaves ◦ Through Xylem Vessels Define the term ‘transpiration’ ◦ Understand that transpiration pull helps to move the water upwards; from the roots to the leaves ◦ Create a water potential gradient 4 Factors affecting transpiration rate
  • 5. What you will learnWhat you will learn (Transport of Food)(Transport of Food) Translocation (Transport of food) ◦ Through Phloem tissue Briefly illustrate translocation through translocation studies ◦ Using aphids ◦ The Ringing Experiment ◦ Using Radio-isotopes Wilting ◦ Advantages and Disadvantages
  • 6. Recall on PhotosynthesisRecall on Photosynthesis How does food get from the leaves to other parts of the plant? How does water get absorb from the soil into and move around the plant?
  • 8. XylemXylem Structures 1.Dead tissue 2.Long and empty lumen 3.Walls are thicken strengthen with lignin Long hollow tube extending from the root to the leaves
  • 9. Development of XylemDevelopment of Xylem Tracheids Xylem vessels Xylem tissue contains Xylem cells (Tracheids) and Xylem vessels
  • 10. Tracheids (Xylem Cells) Long, tapered cells with pitted walls that transport materials upwards At maturity, lignin can be seen deposited at the cell walls and the tracheids gradually dies no cytoplasm Xylem Vessels Long continuous, nonliving hollow tube No cytoplasm Walls are strengthened by lignin
  • 11. LigninLignin Hard and rigid substance Named after the latin word “Lignum”, which means “Wood” Lignin maybe deposited in different ways, giving rise to annular, spiral or pitted xylem vessels
  • 12. XylemXylem 2 Functions a)Conducts water and dissolved minerals from the roots to all other parts of the plant b)Provides mechanical support to the plant due to the presence of lignin 2 Adaptations a)Having a continuous lumen without any protoplasm within to hinder transport of water & dissolved minerals b)Having lignified walls which prevent collapse of the vessels
  • 13. Do you know?Do you know?
  • 14. PhloemPhloem Structures Consists of a column of sieve tubes and companion cells Sieve tubes are separated by sieve plates
  • 15. Sieve TubesSieve Tubes Degenerate Protoplasm No vacuole No nucleus Presence of cytoplasm Cytoplasm remains so that concentration gradient of manufactured food can be maintained
  • 16. Companion CellsCompanion Cells Narrow, thin wall with abundance of cytoplasm and a nucleus Functions a)Keep the sieve tube alive  Provide nutrients a)Contains many mitochondria  Provide energy for translocation sieve plate sieve tube cell companion cell companion cell sieve plate
  • 17. Organization of vascular tissue inOrganization of vascular tissue in StemStem 1. Vascular Bundle The xylem and phloem are grouped together to form the vascular bundles
  • 18. Organization of vascular tissue inOrganization of vascular tissue in StemStem 1. Vascular Bundle 2. Cambium Cambium cells can divide to give rise to new xylem and phloem tissues, hence thickening of the stem Phloem Cambium Xylem
  • 19. Organization of vascular tissue inOrganization of vascular tissue in StemStem 1. Vascular Bundle 2. Cambium The vascular bundles are arranged in a ring surrounding a central region called pith which serves as a storage tissue for food substances 3. Pith
  • 20. Organization of vascular tissue inOrganization of vascular tissue in StemStem 1. Vascular Bundle 2. Cambium The region between the vascular bundles and epidermis is the cortex, which also serves as a storage tissue for food substances 3. Pith 3. Cortex
  • 21. Organization of vascular tissue inOrganization of vascular tissue in StemStem 1. Vascular Bundle 2. Cambium The epidermal cells are covered with a layer of wax called cuticle which prevents excessive loss of water from the stem 3. Pith 4. Cortex 5. Epidermis
  • 22. Organization of vascular tissue inOrganization of vascular tissue in StemStem 1. Vascular Bundle 2. Cambium 3. Pith 4. Cortex 5. Epidermis Phloem Cambium Xylem
  • 23. Organization of vascular tissue inOrganization of vascular tissue in RootRoot 1. Vascular Tissue xylem phloem The xylem and phloem are NOT bundle together. They alternate with each other
  • 24. Organization of vascular tissue inOrganization of vascular tissue in RootRoot 1. Vascular Tissue The region between the epidermis and the endodermis lies the Cortex, which serves as storage tissue 2. Cortex Endodermis
  • 25. Organization of vascular tissue inOrganization of vascular tissue in RootRoot 1. Vascular Tissue The epidermis of the root bearing the root hairs are called the piliferous layer. NO cuticle is present! Why? 2. Cortex 3. Piliferous layer
  • 26. Organization of vascular tissue inOrganization of vascular tissue in RootRoot 1. Vascular Tissue Each root hair is a tubular outgrowth of an epidermal cell. 2. Cortex 3. Piliferous layer 4. Root hair
  • 27. Organization of vascular tissue inOrganization of vascular tissue in RootRoot 1. Vascular Tissue 2. Cortex 3. Piliferous layer 4. Root hair xylem phloem
  • 28. What are the functions and adaptations of Root/ Root hairs? Functions 1.Anchor the plant 2.Specialized in absorption of water & dissolved minerals Adaptations 1.Long and narrow  Increase surface area to volume ratio 2.Cells are alive  Provide energy for active transport 3.Root hairs have cell sap of higher concentration than surrounding soil solution  Cell sap contains sugar, mineral salts which helps to prevent water leaking out of cell. Assist in osmosis
  • 29. Transport StructuresTransport Structures Leaf Stem Root Xylem Xylem Phloem Xylem Phloem Phloem
  • 32. Water uptake in ROOTWater uptake in ROOT Animation
  • 33. Root PressureRoot Pressure Osmotic pressure that build up within the root cells which forces water up the root xylem Recap on Osmosis How does the root maintain a concentration gradient?
  • 34. Root PressureRoot Pressure  Uptake of water by the root hair by Osmosis  Uptake of dissolved minerals through active transport  Cell sap within the root hairs becomes more concentrated than the water in the soil
  • 35. Root PressureRoot Pressure Occurs when the soil moisture level is high either at night or when transpiration is low during the day Can only raise the water in some plants up to 20m Not the main force
  • 36.
  • 37. Water transport in STEMWater transport in STEM You have learnt that water is transported from the root to the stem and the leaf. How does the plant transport water upwards against gravity??
  • 38. TranspirationTranspiration  Evaporation of water from the plant  Loss of water vapor through the stomata on the underside of the leaves 1. The mesophyll cells in the leaves are covered with a thin layer of moisture  Essential for efficient gas exchange to occur 1. Some of this moisture evaporates into the intercellular spaces which diffuses through the stomata into the ‘drier’ air  Water is pulled upwards through osmosis (Transpiration Pull) 1. The waterway in which the water moves from a higher water potential to a lower water potential  Transpiration Stream
  • 39. 4 Environmental Factors that affect4 Environmental Factors that affect TranspirationTranspiration 1. Wind speed 2. Humidity 3. Light intensity 4. Temperature 5. Water supply Animation
  • 40. Light intensityLight intensity During the day, stomata of the leaves open. Why? Photosynthesis!! Gases exchange (CO2 & O2) Water vapor also evaporates (Transpiration)
  • 41. TemperatureTemperature The higher the temperature, the higher the air water capacity to hold moisture At 30ºC, a leaf may transpire 3 times as fast as it does at 20ºC
  • 42. 5 Factors that affect Transpiration5 Factors that affect Transpiration 1. Wind speed ◦ Increases  Increases transpiration rate 1. Humidity ◦ Increases  Decreases transpiration rate 1. Light intensity ◦ Increases  Increases transpiration rate 1. Temperature ◦ Increases  Increases transpiration rate 1. Water supply ◦ Decreases  Decreases transpiration rate
  • 43. PotometerPotometer The rate at which plants take up water depends on the rate of transpiration- the faster a plant transpires, the faster it takes up water.
  • 44. Capillary ActionCapillary Action The attractive force between the molecules of a particular liquid is known as Cohesion Water coheres to each other via chemical bonds called hydrogen bonds (holds the droplets of water together) • The attractive force between two unlike materials is known as Adhesion Adhesion causes water to stick to the inside of the glass When water passes up the thin xylem vessels, it adheres to the surface of the vessels, while the force of osmosis gently ‘pushes’ the water molecules, which cohere to each other, upwards
  • 45.
  • 47. Forces that promotes uptake ofForces that promotes uptake of waterwater 1. Root Pressure  Promotes uptake of water in the root 1. Transpiration pull  Main force to ‘suck’ up the water 1. Capillary Action  Pushes the water upwards due to the adhesion between the walls of the xylem vessels and water molecules as well as the cohesion between the water molecules
  • 49. Transport of Food in plantTransport of Food in plant Food substances (sugar & amino acids) are manufactured in the green leaves through the process called photosynthesis Translocation is the process of transporting food substances downwards from the leaves to all other parts of the plant, through the phloem