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Tilling and ecotilling
- 1. Detection of SNP: TILLING and EcoTilling Presented by : Debadatta Panda
- 4. All SNP areproduct of mutation but all mutations are not SNPs
- 5. Why TILLING ispreferred over others Reverse Genetics approaches
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- 11. In Maize pollenis used
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- 16. Advantage of LiCor Gel system
- 17. TILLING CENTERS ACROSSTHE WORLD
- 18. Year wise TILLINGapproach in various crops
- 19. Examples of TILLINGprojects
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- 21. TILLING and EcoTILLINGtechnique have been adapted in diverse species including rice, maize, Lotus, poplar, Arabidopsis, wheat, barley, potato, tomato, sunflower, common bean, Field Mustard, clover, melon, pea, peanut, sorghum, rapeseed, soybean, melon, poplar, sugarcane, brassica and other for the purpose of gene detection, functional genomics, polymorphism assessment, plant breeding as described in case study part. Current Status of TILLING and EcoTILLING
- 22. EcoTILLING • EcoTILLING issimilar to TILLING, except that its objective is to identify natural genetic variation as opposed to induced mutations. • Many species are not amenable to chemical mutagenesis; therefore, EcoTILLING can aid in the discovery of natural variants and their putative gene function • This approach allows one to rapidly screen through many samples with a gene of interest to identify naturally occurring SNPs and / or small INs/DELS
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- 24. iTILLING • A newapproach to the TILLING method that reduces costs and the time necessary to carry out mutation screening was developed for Arabidopsis and it is called iTILLING, individualized TILLING
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