The document appears to be a collection of slides from various presentations on topics related to biodesign, computational media, and social innovation. Some key points include:
- Presentations on using bacteria to bioremediate uranium contamination and on designing a bioinspired game about environmental remediation.
- A project using DNA to create biological art and an interactive platform for DIY biology.
- The founding of a startup called HumanityX to develop mental healthcare technology and an analytics platform for social good.
- Work with an organization called AWESOME Group on exhibitions combining art, science and technology.
This document discusses a study that used PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) technique to characterize the bacterial communities found in cases of necrosis in primary root canals of children's teeth. The study aimed to identify associations between clinical/radiological data and bacterial profiles. It found polymicrobial communities in all tooth canal samples, with more bands (indicating more bacterial diversity) found in older children and those with caries vs trauma as the cause of infection. While number of bands varied between individuals, there were statistically significant differences based on tooth type, age, and infection cause. The DGGE technique was deemed useful for investigating endodontic microbiota to better understand and treat root canal
This study aims to genotype plankton found in the guts of common Egyptian marine fish and their fry to determine which plankton are useful for feeding them. Researchers will extract metagenomic DNA from the gut contents of wild mullet, amplify the small subunit rRNA gene to fingerprint the microbial species present, perform denaturing gradient gel electrophoresis to separate the DNA, and then sequence and analyze the results to construct phylogenetic trees of the identified microbial species. This technique allows for a more complete profile of the fish gut microbiome compared to traditional microscopy methods. The results could provide insights into improving fish feeding practices.
This study analyzed bacteria in soils from three petroleum contaminated sites in Nigeria using metagenomic techniques. Soil samples were collected from depths of 17-20cm and 37-40cm and analyzed for physicochemical properties and bacterial diversity via 16S rRNA gene sequencing. The sequences showed diversity of Proteobacteria, Actinobacteria, Firmicutes and Acidobacteria. Differences in bacterial profiles between depths were found at one site but not the other two. Heavy metals like zinc and lead were present at high levels. The study demonstrated the impact of anthropogenic activities and soil properties on bacterial communities in contaminated soils.
Microbial community analysis in anaerobic palm oil mill effluent (pome) waste...eSAT Journals
Microorganisms play a key role in wastewater bio-treatment processes and understanding the microbial community structure is of great importance to improve treatment performance. Denaturing gradient gel electrophoresis (DGGE) was used to monitor succession of the microbial community and thus predominant bands were sequenced to reveal the microbial community composition inside palm oil mill effluent (POME) wastewater.DNA bands from DGGE gels were excised with a sterile blade and placed in 1.5 ml eppendorf tube containing 50 μl deionized water (ddH2O). Tubes were incubated overnight at 4C to elute the DNA. Eluted DNA was purified using QIAquick gel extraction kits (QIAGEN, Inc., Valencia, CA) and was frozen and thawed three times.Microbial DNA successfully excised and purified from DGGE was amplified using polymerase chain reaction (PCR).Five micro liters of the supernatant were used as a template to re-amplify the DNA using 16s rDNA primers,341f (with no GC-clamp) (5'- cct-acg-gga-ggc-agc-ag-3') and reverse(r) primers 907r (5'-ccc-cgt-caa-ttc-att-tga-gtt-t-3'). Amplification was repeated referring to the steps in ‘PCR amplification of 16s rDNA’. PCR products from agarose gels were cut and purified using QIAquick Gel Extraction Kit (QIAGEN, Inc., Valencia, CA), which were similar to the purification steps after recovery of DNA from DGGE, and sequenced in both directions with the same primers (with no GC-clamp) as used in PCR. Moreover, start-up is an important step in establishing proper community structure in all kinds of biological treatment processes. In anaerobic POME wastewater, 6 sequences of Firmicutes, 5 sequence of Proteobacterium and 2 sequences of Bacteroidetes were found through denaturing gradient gel electrophoresis (DGGE) results. Sequence closely related to Rummeliibacillus suwonensis strain G20 was detected grows at band BE10, BE11, BE12, BE15, BE16, BE17 and BE18. Meanwhile BE25 and BE26 were found at band after treatment process done.Rummeliibacillus suwonensis is an aerobic, Gram-positive, rod shaped, round-spore-forming bacteria which were isolated from aerobic condition. Keywords: Palm oil mill effluent (POME), anaerobic POME, Denaturing gradient gel electrophoresis (DGGE), and microbial community.
This document discusses an experiment to isolate bacteria from soil and foam samples that are able to degrade polystyrene foam. The key steps involved growing bacteria from the samples in a nutrient broth containing polystyrene foam. Bacterial DNA was extracted weekly and analyzed using PCR and gel electrophoresis to identify dominant bacterial species over time. Scanning electron microscopy images show signs of degradation on polystyrene exposed to bacteria from soil and foam samples, but not the control. This suggests bacteria isolated from these environmental samples have the ability to break down polystyrene foam.
Shell microstructures of Cambrian molluscs replicated by phosphateأسعد لحمر
This article discusses shell microstructures of Cambrian molluscs that have been replicated by phosphate deposits. The original aragonitic shells are usually destroyed over time, but phosphate coats and fills can preserve the microstructure at high magnifications. Many common molluscan microstructures like prismatic aragonite, nacre, and foliated calcite are found to have evolved by the Middle Cambrian in representatives of the class Monoplacophora. The discovery of these replicated microstructures provides new insights into the early evolution of molluscan shells.
A 70-year-old man was hospitalized with bacteremia and acute cholecystitis. Blood and gallbladder samples grew gram-positive rods that were identified as Lactobacillus salivarius using 16S rRNA gene sequencing, but various phenotypic methods gave inconsistent or incorrect results. The study evaluated using 16S rRNA gene sequencing for identifying gram-positive rods in the clinical laboratory, finding it more accurate and able to identify organisms that phenotypic methods could not.
This document discusses a study that used PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) technique to characterize the bacterial communities found in cases of necrosis in primary root canals of children's teeth. The study aimed to identify associations between clinical/radiological data and bacterial profiles. It found polymicrobial communities in all tooth canal samples, with more bands (indicating more bacterial diversity) found in older children and those with caries vs trauma as the cause of infection. While number of bands varied between individuals, there were statistically significant differences based on tooth type, age, and infection cause. The DGGE technique was deemed useful for investigating endodontic microbiota to better understand and treat root canal
This study aims to genotype plankton found in the guts of common Egyptian marine fish and their fry to determine which plankton are useful for feeding them. Researchers will extract metagenomic DNA from the gut contents of wild mullet, amplify the small subunit rRNA gene to fingerprint the microbial species present, perform denaturing gradient gel electrophoresis to separate the DNA, and then sequence and analyze the results to construct phylogenetic trees of the identified microbial species. This technique allows for a more complete profile of the fish gut microbiome compared to traditional microscopy methods. The results could provide insights into improving fish feeding practices.
This study analyzed bacteria in soils from three petroleum contaminated sites in Nigeria using metagenomic techniques. Soil samples were collected from depths of 17-20cm and 37-40cm and analyzed for physicochemical properties and bacterial diversity via 16S rRNA gene sequencing. The sequences showed diversity of Proteobacteria, Actinobacteria, Firmicutes and Acidobacteria. Differences in bacterial profiles between depths were found at one site but not the other two. Heavy metals like zinc and lead were present at high levels. The study demonstrated the impact of anthropogenic activities and soil properties on bacterial communities in contaminated soils.
Microbial community analysis in anaerobic palm oil mill effluent (pome) waste...eSAT Journals
Microorganisms play a key role in wastewater bio-treatment processes and understanding the microbial community structure is of great importance to improve treatment performance. Denaturing gradient gel electrophoresis (DGGE) was used to monitor succession of the microbial community and thus predominant bands were sequenced to reveal the microbial community composition inside palm oil mill effluent (POME) wastewater.DNA bands from DGGE gels were excised with a sterile blade and placed in 1.5 ml eppendorf tube containing 50 μl deionized water (ddH2O). Tubes were incubated overnight at 4C to elute the DNA. Eluted DNA was purified using QIAquick gel extraction kits (QIAGEN, Inc., Valencia, CA) and was frozen and thawed three times.Microbial DNA successfully excised and purified from DGGE was amplified using polymerase chain reaction (PCR).Five micro liters of the supernatant were used as a template to re-amplify the DNA using 16s rDNA primers,341f (with no GC-clamp) (5'- cct-acg-gga-ggc-agc-ag-3') and reverse(r) primers 907r (5'-ccc-cgt-caa-ttc-att-tga-gtt-t-3'). Amplification was repeated referring to the steps in ‘PCR amplification of 16s rDNA’. PCR products from agarose gels were cut and purified using QIAquick Gel Extraction Kit (QIAGEN, Inc., Valencia, CA), which were similar to the purification steps after recovery of DNA from DGGE, and sequenced in both directions with the same primers (with no GC-clamp) as used in PCR. Moreover, start-up is an important step in establishing proper community structure in all kinds of biological treatment processes. In anaerobic POME wastewater, 6 sequences of Firmicutes, 5 sequence of Proteobacterium and 2 sequences of Bacteroidetes were found through denaturing gradient gel electrophoresis (DGGE) results. Sequence closely related to Rummeliibacillus suwonensis strain G20 was detected grows at band BE10, BE11, BE12, BE15, BE16, BE17 and BE18. Meanwhile BE25 and BE26 were found at band after treatment process done.Rummeliibacillus suwonensis is an aerobic, Gram-positive, rod shaped, round-spore-forming bacteria which were isolated from aerobic condition. Keywords: Palm oil mill effluent (POME), anaerobic POME, Denaturing gradient gel electrophoresis (DGGE), and microbial community.
This document discusses an experiment to isolate bacteria from soil and foam samples that are able to degrade polystyrene foam. The key steps involved growing bacteria from the samples in a nutrient broth containing polystyrene foam. Bacterial DNA was extracted weekly and analyzed using PCR and gel electrophoresis to identify dominant bacterial species over time. Scanning electron microscopy images show signs of degradation on polystyrene exposed to bacteria from soil and foam samples, but not the control. This suggests bacteria isolated from these environmental samples have the ability to break down polystyrene foam.
Shell microstructures of Cambrian molluscs replicated by phosphateأسعد لحمر
This article discusses shell microstructures of Cambrian molluscs that have been replicated by phosphate deposits. The original aragonitic shells are usually destroyed over time, but phosphate coats and fills can preserve the microstructure at high magnifications. Many common molluscan microstructures like prismatic aragonite, nacre, and foliated calcite are found to have evolved by the Middle Cambrian in representatives of the class Monoplacophora. The discovery of these replicated microstructures provides new insights into the early evolution of molluscan shells.
A 70-year-old man was hospitalized with bacteremia and acute cholecystitis. Blood and gallbladder samples grew gram-positive rods that were identified as Lactobacillus salivarius using 16S rRNA gene sequencing, but various phenotypic methods gave inconsistent or incorrect results. The study evaluated using 16S rRNA gene sequencing for identifying gram-positive rods in the clinical laboratory, finding it more accurate and able to identify organisms that phenotypic methods could not.
Aquaculture microbiology and biotechnology vol (1)أسعد لحمر
This chapter discusses transgenic fish and the applications of genetic engineering in aquaculture. Specifically, it describes how genetic engineering techniques like chromosome manipulation and hormone treatment are currently used to produce sterile and monosex fish lines. It also outlines how researchers are using transgenic methods to develop fish with desirable traits like increased growth rates, improved feed conversion efficiency, disease resistance, and tolerance to stressful environmental conditions. However, the release of transgenic fish into the environment has raised ecological and human health concerns from some groups. The chapter examines both the potential benefits of transgenic fish for aquaculture as well as some of the criticisms against this technology.
This document summarizes information about human cell formation and the PAX6 gene. It discusses how genes, DNA, transcription factors, and mRNA are involved in transcription and translation. It provides details on the specific location of the PAX6 gene on chromosome 11 between base pairs 31,784,792 to 31,817,961. It also lists references used for the information presented.
The document discusses building the right team to develop new products and ideas without knowing the next big thing. It recommends assembling a diverse dream team of experienced and expert leaders in UX, UI, iOS, Android, front-end, back-end development, marketing, and business development who are over 35 years old. The document estimates the funding needed for such a team at $5 million annually for 3 years.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive functioning. Exercise boosts blood flow and levels of neurotransmitters and endorphins which elevate and stabilize mood.
Nano-biotechnology is a promising field of interdisciplinary research. It is a novel scientific approach that involves the use of materials and equipment capable of manipulating physical as well as chemical properties of a substance at molecular levels. Nanoparticles present an extremely gorgeous platform for a diverse range of biological applications. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. Nanotechnology is ubiquitous and pervasive. It is an emerging field in all areas of science, engineering and technology. The marriage between nanotechnology and biotechnology yields a new class of nanostructured hybrid patterns that will be very efficient and useful as environmental adsorbents, absorbent materials and adhesion materials in environment.
This are can endeavour to provide and fundamentally streamline the technologies currently used in environmental detection, sensing and remediation. Rapid testing technologies and biosensors related to the control of pests and cross contamination of agriculture and food products will lead to applications of nanotechnology in the near future. These include agricultural productivity enhancement involving nanoporous zeolites for slow release and efficient dosage of water and fertilizer, nanocapsules for herbicide delivery and vector and pest management and nanosensors for pest detection. Most investment is made primarily in developed countries; research advancements provide glimpses of potential applications in agriculture. This overview is concentrated on modern strategies used for the management of water, pesticides, limitations in the use of chemical pesticides and potential of nano-materials in sustainable agriculture management as modern approaches of nano-biotechnology.
The outlook of nano-bioscience in agriculture is vague owing to a lot of grounds, for example, the unconstructive response from people towards genetically modified (GM) crops, need of a lot of required cleverness in government agricultural research and technology units for nano type of explorations and poorly-equipped new instruments and new-fangled technologies. Welcome to Nano World.
1. Synthetic biology enables the extreme genetic engineering of lifeforms through techniques like designing DNA, splicing genes, and synthesizing genomes.
2. There are concerns about potential misuse for biowarfare and rapid digital biopiracy that can bypass traditional benefit-sharing systems.
3. As synthetic biology advances, it may allow for the mass construction of new lifeforms and genomes at an unprecedented scale and speed, with uncertain and potentially dangerous implications.
The document discusses the challenges of monitoring ecological changes in a rapidly warming world with increasing population pressures. It argues that we can no longer rely on low-resolution ecological models and need to track environmental data at high frequencies and spatial resolutions over long periods of time. The TERN/ACEAS network is well-positioned to establish continent-wide monitoring and facilitate collaborative use of large ecological datasets. Advances in remote sensing, sensor networks, and phenomics now allow tracking environmental variables and organism phenotypes in high detail. This level of data collection will be essential to understand and minimize impacts of climate change on ecosystems and food production over the coming century.
Sensors and Internet of Things: the role of nanostructured semiconductorsSalvo Mirabella
Future trends in materials science and nanotechnology for innovative sensing devices required within IoT.
Some visions on how IoT will impact our daily life and some materials research on novel nanostructures based enabling technologies
This document discusses research on using a hydrogen-based membrane biofilm reactor (MBfR) to bioreduce uranium from contaminated groundwater. Key points:
- Researchers used an MBfR system fed with 80% hydrogen and 20% carbon dioxide to stimulate bacteria that can bioreduce uranium from 60 μg/L to below the EPA limit of 30 μg/L.
- The system included a circulator, sensors, and medium containing uranium that was monitored over time. Uranium concentrations decreased over time and after changing the medium.
- By harnessing bacteria in an MBfR system, this approach aims to cost-effectively remediate uranium-contaminated
This presentation explores the connection between Sciences and Arts through Pat's interdisciplinary research projects (Freshmen @ASU2015) at Arizona State University and his past years in Thailand.
Nanotechnology has the potential to drastically change many industries such as electronics, medicine, and the environment. It may allow for the construction of smaller computer circuits that run faster, biological nanosensors for diagnostics, and nanomaterials that increase fuel cell efficiency. Some believe that molecular manufacturing could realize the concept of replicators seen in Star Trek, allowing any physical object to be produced on demand through the precise arrangement of individual atoms by nanomachines. While still in development, 3D printing provides a similar capability today through the layer-by-layer extrusion of materials like plastic, wood, and even some foods to create physical objects based on digital designs.
Synthetic biology aims to manufacture biology digitally through techniques like DNA synthesis and genetic engineering. This allows designing and constructing new biological parts, devices and systems for useful purposes. Some key points discussed are:
1) Synthetic biology is advancing manufacturing through precision biology and digital farming using techniques like genome reading, DNA synthesis and robotic genome construction.
2) It is enabling new industrial routes for compounds like through biosynthetic production, and disrupting existing industries like agriculture, chemicals and pharma.
3) Long term implications discussed include the potential for synthetic biology techniques to replace natural production systems and enable more intensive and extractive farming systems globally.
What does it mean to be a bioinformatician?Dmytro Fishman
This document describes Dmytro Fishman's interests which include functional genomics, medical imaging, medical signals, autoimmunity, and teaching. It provides examples of his work applying machine learning and deep learning techniques to problems in these areas, such as using convolutional neural networks for disease prediction from medical images and signal processing of EEG and heart data. Collaborators on some of these projects are also mentioned.
33D Printing Organ on a Chip, Jan Eite Bullema, TNO Industrial Science
The goal of this so-called deep dive exploration is to identify business potential of biomimetic microfluidic systems (organ-on-a-chip).
One of the most attractive applications of organ-on-a-chip at the moment appears to be mimicking human’s physiological responses for medicine development.
Efficacy of medicine is a big challenge for the pharmaceutical industry. Depending on the illness specific drugs can have an efficacy of less than 30 %.
Drug efficacy is one of the topics addressed by the Netherlands by an "Over de grenzen" KNAW program.
In the presentation I will focus on recent -3D Printing developments- in the field of organ / organ-on-a-chip printing. Just to give an impression of the awesome, fantastic, amazing, wow - no - WOW!!- developments. Since a few years organs are printed in the lab, and I will start with some examples of printed organs bones, kidneys, blood vesels, livers, ears, that can be made at the moment. Then I will dive deeper into organ-on-a-chip, a true micro sysmtems topic - my area of expertise here- , and explain a little on what organs-on-chip are. Subsequent I will go into various technologies for 3D printing of cell and bio materials. And I will finish with some ideas on organ printing that are trully amazing, most impressive are Craig Venter's .
Datamining Soundscaoes for Orthoptera songs: Big Data or Data Deluge?
Talk by Klaus Riede at 12th International Congress of Orthopterology http://www.ico2016.com.br/scientific-program/
on 3rd Nov 2016, within
Symposium: Acoustic and vibrational communication in Orthoptera
Organizer: Fernando Montealegre Zapata
This document provides an overview of the speaker's experiences with DIY biology and biohacking. It discusses the development of generic laboratory infrastructure to support citizen science, knowledge sharing and open source hardware. It describes various workshops and labs focused on transdisciplinary approaches at the intersection of biology, art, and design. Examples include DIY microscopy, synthetic biology competitions, environmental monitoring, and body hacking. The document emphasizes that biohacking has always existed as people find novel ways to apply science in their daily lives.
Soil quality and open science presentation april2014Tom Olijhoek
This document discusses skills and knowledge related to soil science, microbiology, and sharing information openly. It covers:
- Key skills including biochemistry, geomicrobiology, and open science practices
- How soil microbes like bacteria and fungi decompose organic matter and make nutrients available to plants through the soil food web
- The roles of different soil microbes in processes like nitrogen fixation and decomposition
- The potential to discover new bacteria through astrobiology and apply findings to agriculture
- Promoting open sharing of agricultural knowledge through networks and grassroots initiatives using emerging technologies
This document provides instructions for extracting DNA from a banana in a kitchen. The protocol involves:
1. Creating a saline solution and cutting the banana.
2. Adding the banana pieces to a plastic bag with saline, dish soap, and contact lens solution.
3. Mash the banana mixture and filter it into a test tube.
4. Transfer some of the filtered solution to a microtube using a pipette.
5. Add ethanol to the test tube and microtube to precipitate out the banana's DNA.
Aquaculture microbiology and biotechnology vol (1)أسعد لحمر
This chapter discusses transgenic fish and the applications of genetic engineering in aquaculture. Specifically, it describes how genetic engineering techniques like chromosome manipulation and hormone treatment are currently used to produce sterile and monosex fish lines. It also outlines how researchers are using transgenic methods to develop fish with desirable traits like increased growth rates, improved feed conversion efficiency, disease resistance, and tolerance to stressful environmental conditions. However, the release of transgenic fish into the environment has raised ecological and human health concerns from some groups. The chapter examines both the potential benefits of transgenic fish for aquaculture as well as some of the criticisms against this technology.
This document summarizes information about human cell formation and the PAX6 gene. It discusses how genes, DNA, transcription factors, and mRNA are involved in transcription and translation. It provides details on the specific location of the PAX6 gene on chromosome 11 between base pairs 31,784,792 to 31,817,961. It also lists references used for the information presented.
The document discusses building the right team to develop new products and ideas without knowing the next big thing. It recommends assembling a diverse dream team of experienced and expert leaders in UX, UI, iOS, Android, front-end, back-end development, marketing, and business development who are over 35 years old. The document estimates the funding needed for such a team at $5 million annually for 3 years.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive functioning. Exercise boosts blood flow and levels of neurotransmitters and endorphins which elevate and stabilize mood.
Nano-biotechnology is a promising field of interdisciplinary research. It is a novel scientific approach that involves the use of materials and equipment capable of manipulating physical as well as chemical properties of a substance at molecular levels. Nanoparticles present an extremely gorgeous platform for a diverse range of biological applications. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. Nanotechnology is ubiquitous and pervasive. It is an emerging field in all areas of science, engineering and technology. The marriage between nanotechnology and biotechnology yields a new class of nanostructured hybrid patterns that will be very efficient and useful as environmental adsorbents, absorbent materials and adhesion materials in environment.
This are can endeavour to provide and fundamentally streamline the technologies currently used in environmental detection, sensing and remediation. Rapid testing technologies and biosensors related to the control of pests and cross contamination of agriculture and food products will lead to applications of nanotechnology in the near future. These include agricultural productivity enhancement involving nanoporous zeolites for slow release and efficient dosage of water and fertilizer, nanocapsules for herbicide delivery and vector and pest management and nanosensors for pest detection. Most investment is made primarily in developed countries; research advancements provide glimpses of potential applications in agriculture. This overview is concentrated on modern strategies used for the management of water, pesticides, limitations in the use of chemical pesticides and potential of nano-materials in sustainable agriculture management as modern approaches of nano-biotechnology.
The outlook of nano-bioscience in agriculture is vague owing to a lot of grounds, for example, the unconstructive response from people towards genetically modified (GM) crops, need of a lot of required cleverness in government agricultural research and technology units for nano type of explorations and poorly-equipped new instruments and new-fangled technologies. Welcome to Nano World.
1. Synthetic biology enables the extreme genetic engineering of lifeforms through techniques like designing DNA, splicing genes, and synthesizing genomes.
2. There are concerns about potential misuse for biowarfare and rapid digital biopiracy that can bypass traditional benefit-sharing systems.
3. As synthetic biology advances, it may allow for the mass construction of new lifeforms and genomes at an unprecedented scale and speed, with uncertain and potentially dangerous implications.
The document discusses the challenges of monitoring ecological changes in a rapidly warming world with increasing population pressures. It argues that we can no longer rely on low-resolution ecological models and need to track environmental data at high frequencies and spatial resolutions over long periods of time. The TERN/ACEAS network is well-positioned to establish continent-wide monitoring and facilitate collaborative use of large ecological datasets. Advances in remote sensing, sensor networks, and phenomics now allow tracking environmental variables and organism phenotypes in high detail. This level of data collection will be essential to understand and minimize impacts of climate change on ecosystems and food production over the coming century.
Sensors and Internet of Things: the role of nanostructured semiconductorsSalvo Mirabella
Future trends in materials science and nanotechnology for innovative sensing devices required within IoT.
Some visions on how IoT will impact our daily life and some materials research on novel nanostructures based enabling technologies
This document discusses research on using a hydrogen-based membrane biofilm reactor (MBfR) to bioreduce uranium from contaminated groundwater. Key points:
- Researchers used an MBfR system fed with 80% hydrogen and 20% carbon dioxide to stimulate bacteria that can bioreduce uranium from 60 μg/L to below the EPA limit of 30 μg/L.
- The system included a circulator, sensors, and medium containing uranium that was monitored over time. Uranium concentrations decreased over time and after changing the medium.
- By harnessing bacteria in an MBfR system, this approach aims to cost-effectively remediate uranium-contaminated
This presentation explores the connection between Sciences and Arts through Pat's interdisciplinary research projects (Freshmen @ASU2015) at Arizona State University and his past years in Thailand.
Nanotechnology has the potential to drastically change many industries such as electronics, medicine, and the environment. It may allow for the construction of smaller computer circuits that run faster, biological nanosensors for diagnostics, and nanomaterials that increase fuel cell efficiency. Some believe that molecular manufacturing could realize the concept of replicators seen in Star Trek, allowing any physical object to be produced on demand through the precise arrangement of individual atoms by nanomachines. While still in development, 3D printing provides a similar capability today through the layer-by-layer extrusion of materials like plastic, wood, and even some foods to create physical objects based on digital designs.
Synthetic biology aims to manufacture biology digitally through techniques like DNA synthesis and genetic engineering. This allows designing and constructing new biological parts, devices and systems for useful purposes. Some key points discussed are:
1) Synthetic biology is advancing manufacturing through precision biology and digital farming using techniques like genome reading, DNA synthesis and robotic genome construction.
2) It is enabling new industrial routes for compounds like through biosynthetic production, and disrupting existing industries like agriculture, chemicals and pharma.
3) Long term implications discussed include the potential for synthetic biology techniques to replace natural production systems and enable more intensive and extractive farming systems globally.
What does it mean to be a bioinformatician?Dmytro Fishman
This document describes Dmytro Fishman's interests which include functional genomics, medical imaging, medical signals, autoimmunity, and teaching. It provides examples of his work applying machine learning and deep learning techniques to problems in these areas, such as using convolutional neural networks for disease prediction from medical images and signal processing of EEG and heart data. Collaborators on some of these projects are also mentioned.
33D Printing Organ on a Chip, Jan Eite Bullema, TNO Industrial Science
The goal of this so-called deep dive exploration is to identify business potential of biomimetic microfluidic systems (organ-on-a-chip).
One of the most attractive applications of organ-on-a-chip at the moment appears to be mimicking human’s physiological responses for medicine development.
Efficacy of medicine is a big challenge for the pharmaceutical industry. Depending on the illness specific drugs can have an efficacy of less than 30 %.
Drug efficacy is one of the topics addressed by the Netherlands by an "Over de grenzen" KNAW program.
In the presentation I will focus on recent -3D Printing developments- in the field of organ / organ-on-a-chip printing. Just to give an impression of the awesome, fantastic, amazing, wow - no - WOW!!- developments. Since a few years organs are printed in the lab, and I will start with some examples of printed organs bones, kidneys, blood vesels, livers, ears, that can be made at the moment. Then I will dive deeper into organ-on-a-chip, a true micro sysmtems topic - my area of expertise here- , and explain a little on what organs-on-chip are. Subsequent I will go into various technologies for 3D printing of cell and bio materials. And I will finish with some ideas on organ printing that are trully amazing, most impressive are Craig Venter's .
Datamining Soundscaoes for Orthoptera songs: Big Data or Data Deluge?
Talk by Klaus Riede at 12th International Congress of Orthopterology http://www.ico2016.com.br/scientific-program/
on 3rd Nov 2016, within
Symposium: Acoustic and vibrational communication in Orthoptera
Organizer: Fernando Montealegre Zapata
This document provides an overview of the speaker's experiences with DIY biology and biohacking. It discusses the development of generic laboratory infrastructure to support citizen science, knowledge sharing and open source hardware. It describes various workshops and labs focused on transdisciplinary approaches at the intersection of biology, art, and design. Examples include DIY microscopy, synthetic biology competitions, environmental monitoring, and body hacking. The document emphasizes that biohacking has always existed as people find novel ways to apply science in their daily lives.
Soil quality and open science presentation april2014Tom Olijhoek
This document discusses skills and knowledge related to soil science, microbiology, and sharing information openly. It covers:
- Key skills including biochemistry, geomicrobiology, and open science practices
- How soil microbes like bacteria and fungi decompose organic matter and make nutrients available to plants through the soil food web
- The roles of different soil microbes in processes like nitrogen fixation and decomposition
- The potential to discover new bacteria through astrobiology and apply findings to agriculture
- Promoting open sharing of agricultural knowledge through networks and grassroots initiatives using emerging technologies
This document provides instructions for extracting DNA from a banana in a kitchen. The protocol involves:
1. Creating a saline solution and cutting the banana.
2. Adding the banana pieces to a plastic bag with saline, dish soap, and contact lens solution.
3. Mash the banana mixture and filter it into a test tube.
4. Transfer some of the filtered solution to a microtube using a pipette.
5. Add ethanol to the test tube and microtube to precipitate out the banana's DNA.
UGM 2022: Open Source Biological Art and DIY / DIWO Scientific InstrumentsMarc Dusseiller Dusjagr
Overview of transdisciplinary approach bridging art and science in the global hackteria network.
Concrete examples of building low-cost scientific laboratory equiment.
Various DIY electronics for data logging.
what is art?
thGAP - BAbyss in Moderno!! Transgenic Human Germline Alternatives ProjectMarc Dusseiller Dusjagr
thGAP - Transgenic Human Germline Alternatives Project, presents an evening of input lectures, discussions and a performative workshop on artistic interventions for future scenarios of human genetic and inheritable modifications.
To begin our lecturers, Marc Dusseiller aka "dusjagr" and Rodrigo Martin Iglesias, will give an overview of their transdisciplinary practices, including the history of hackteria, a global network for sharing knowledge to involve artists in hands-on and Do-It-With-Others (DIWO) working with the lifesciences, and reflections on future scenarios from the 8-bit computer games of the 80ies to current real-world endeavous of genetically modifiying the human species.
We will then follow up with discussions and hands-on experiments on working with embryos, ovums, gametes, genetic materials from code to slime, in a creative and playful workshop setup, where all paticipant can collaborate on artistic interventions into the germline of a post-human future.
This proposal outlines a novel genetic circuit that could be inserted into E. coli to detect safe and harmful concentrations of lead in liquid samples. The circuit would utilize existing lead-binding proteins and promoters, as well as common metabolic signals, fluorescent reporters, and terminator sequences. It is composed of three modules: a concentration detector, memory unit, and signal amplifying fluorescent reporter. While the actual circuit cannot be constructed yet, computer simulations show it could function as intended given the appropriate biological parts. The proposal provides detailed specifications and simulations of each module and the complete circuit.
Prof Derek Clements-Croome - Challenges and opportunities for intelligent bui...Derek Clements-Croome
Professor Derek Clements-Croome gave a presentation on "Challenges and Opportunities for Green Intelligent Buildings in the 21st Century" at Dundee University. The presentation covered several topics:
1) Drivers of change for buildings including population, sustainability, health, and innovation.
2) Technologies that will impact buildings like sensors, nanotechnology, IT and communications.
3) Environmental issues like climate change, carbon emissions, and renewable energy potentials.
4) Design strategies for green buildings around orientation, insulation, solar gain, and efficient cooling/heating systems.
Our team is developing a technique called biOrigami to reduce the mass, volume, and assembly time of materials for space missions. We are producing biologically-based substrate materials like polystyrene and poly-(3-hydroxybutyrate) plastics and cellulose, engineering folding mechanisms using these materials, and designing functional products. We are also creating a novel and efficient method of bacterial transformation using CRISPR/Cas9 to selectively insert genes. This project integrates manufacturing at both the micro and macro levels to create lightweight, compactly foldable materials for applications in space exploration.
"The Quest for A field Guide to the Microbes" talk by Jonathan Eisen February...Jonathan Eisen
The document discusses the author's quest to create a field guide to microbes. It describes the challenges in doing so given microbes' small size and high diversity. The author discusses using DNA sequencing and phylogenetic trees to identify microbes and determine their functions and relationships. Examples are given of using DNA to study human microbiomes, forensic analysis, and microbial communities. The need for a comprehensive field guide is argued to better understand the roles and identification of microbes.
Explore interdisciplinary approach on designing social robot (fro Biology to Performance art). An introduction lecture at the Social Robot Design workshop at Junior Science Talent Project (JSTP) camp.
Biodegradation of Polystyrene foam by the Microorganism from LandfillPat Pataranutaporn
This document summarizes a research project on biodegrading polystyrene foam. The project aimed to identify microbes from a landfill that can use polystyrene as a sole carbon source. Microbes were sampled from styrofoam and soil in the landfill. Community analysis identified several bacterial species growing on polystyrene, including Caulobacter segnis, Massilia aerilata, and Herbaspirillum seropedicae. Scanning electron microscopy showed signs of polystyrene degradation by microbes from styrofoam and soil. The research suggests certain landfill microbes are capable of biodegrading polystyrene.
This document appears to be a presentation about isolating bacteria from contaminated soil that can degrade polystyrene foam. It describes collecting soil samples, growing bacteria in a mineral medium with polystyrene foam as the sole carbon source, and isolating 24 gram-positive bacterial isolates, 19 of which were rod-shaped and 5 round-shaped. Traces of degradation were seen on the polystyrene foam in the experimental flasks compared to the controls. Further study is needed to confirm these bacteria can degrade polystyrene foam.
The document describes a 48-hour hackathon called HumanityX that brings together technology experts to develop innovations for improving mental health and suicide prevention. A team is working on a system that uses machine learning to identify suicidal messages on social media and connects those users to mental health professionals for help. The goal of HumanityX is to apply technology solutions to save lives and support humanity.
The document appears to be a collection of slides in Thai about various topics related to coding, innovation, and dreams. Some of the slides discuss coding and its importance, breaking down dreams into achievable steps, looking to examples for inspiration, and not needing to start projects from scratch. Other slides provide examples of innovative projects like a friendly robot, an artificial intelligence system to identify suicidal social media posts, and using plants to detoxify dangerous chemicals. The collection encourages dreaming big but making dreams achievable through hard work and learning from others.
Innovation + Aesthetics in Computational and Biological EraPat Pataranutaporn
This document discusses various projects at the intersection of biodesign, interactive art, and social innovation. It describes projects such as using bacteria to bioremediate uranium, an interactive game about uranium bioremediation, creating art with DNA, using plants to phytoremediate brownfields, an open DIY biology platform, developing a food product called JUBE to address malnutrition, 3D printing Thai food, and using technology to help with mental health crises. The document emphasizes innovation to help humanity.
Pat Pataranutporn is a faculty member at Arizona State University who works on multidisciplinary projects related to data science, fine arts, cultural preservation, architecture, futuristic technology, interactive media, creative bioinformatics, and biodesign. Their research interests include structural DNA nanotechnology, self-replication and dynamic molecular assembly, molecular design and biomimetics, infectious diseases and vaccinology, uranium bioremediation, and more. They have collaborated with various universities and organizations on these topics.
Pat presented several of their projects including The Bioremediation Game and computer vision and idea development projects. The Bioremediation Game teaches kids about detoxifying chemicals and was well-received. Pat also discussed their mentor Prof. Savaporn Supaphol and encouraged attendees that inspiration can come from many places and that perfect projects are not the most important thing. Breaking problems down and finding the right tools are important for success in creating projects.
Pat presents Thailand and shares some of its secrets treasures. He introduces aspects of Thai culture like its biodiversity, agriculture, food, textiles, people and more. Pat expresses pride in his home country and invites the reader to learn about Thailand's exotic inspirations through its natural beauty, cultural heritage and warm people.
Pat Pataranutaporn is an undergraduate student at Arizona State University studying biological sciences, with a focus on environmental biotechnology and interactive media. He has a range of skills including software development, simulation design, molecular biology techniques, and biomimicry thinking. Pat is a research fellow, social media chair, and cofounder of startups working at the intersection of biology, technology, and social innovation.
This document summarizes a research study on the biodegradation of polystyrene foam by microorganisms found in a landfill in Thailand. Samples of microbes were taken from polystyrene foam and contaminated soil in the landfill. The microbes were cultured with polystyrene as the sole carbon source to identify those capable of degrading it. Analysis of the cultures over time found shifts in the dominant microbial species. Selected species were identified through molecular techniques as known aromatic compound degraders, including Herbaspirillum seropedicae and Ochrobactrum sp. Scanning electron microscopy was used to observe physical changes to polystyrene exposed to the cultures, indicating biodegradation
Immersive Learning That Works: Research Grounding and Paths ForwardLeonel Morgado
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
Authoring a personal GPT for your research and practice: How we created the Q...Leonel Morgado
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...Sérgio Sacani
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
2. www.HumanityX.tech www.BioX.tech
T h e F u t u r e o f i n n o v a t i o n i s
Pat Pataranutaporn
Future
Present
Past
Research Entrepreneurship Liberal Arts
Knowledges?
Music by Justin Timberlake
3. www.HumanityX.tech www.BioX.tech
T h e F u t u r e o f i n n o v a t i o n i s
Pat Pataranutaporn
Future
Present
Past
Research Entrepreneurship Liberal Arts
Knowledges?
Music by Justin Timberlake
51. Recycling &
Incineration
1%turn back to polystyrene
most of the polystyrene is converted
into carbon dioxide, water vapor, and heat.
"Ease of Disposal". Retrieved 2009-06-25
88. United Nations Scientific
Committee on the
Effects of Atomic
Radiation
URANIUM
Uranium is weakly radioactive, but
highly toxic once inhaled or ingested.
It can be found in low levels within all
rock, soil, and water.
Biodesign
Computational
media
Social innovation
89. United Nations Scientific
Committee on the
Effects of Atomic
Radiation
Depleted
Uranium
exposureleukemogenic
effect
genetic
effect
reproductive
effect
neurological
effect
Miller AC, McClain D.; McClain (Jan–Mar
2007). "A review of depleted uranium
biological effects: in vitro and in vivo studies".
Rev Environ Health
Biodesign
Computational
media
Social innovation
90. Bioreduction of Uranium using a Hydrogen-based
membrane biofilm reactor at Night of the open door
Biodesign
Computational
media
Social innovation
91. Bioreduction of Uranium using a Hydrogen-based
membrane biofilm reactor at Night of the open door
Biodesign
Computational
media
Social innovation
92. Bioreduction of Uranium using a Hydrogen-based
membrane biofilm reactor at Night of the open door
Biodesign
Computational
media
Social innovation
120. Biodesign
Computational
media
Social innovation
Pat Pataranutaporn at the South by Southwest Eco Conference.
Pataranutaporn and his team were invited to attend and give a
presentation on their biomimicry proposal as a part of winning
second place in the global competition.
132. Biodesign
Computational
media
Social innovation
Jordan Bates Ram Polur Pat PataranutapornBin HongLeeDr. Kacie McCollum
President & CEODirector of Operations Director of InnovationDirector of
Clinical Informatics
Director of Product
Development & Outreach
272. www.HumanityX.tech www.BioX.tech
T h e F u t u r e o f i n n o v a t i o n i s
Pat Pataranutaporn
Future
Present
Past
Research Entrepreneurship Liberal Arts
Knowledges?
Music by Justin Timberlake
273. www.HumanityX.tech www.BioX.tech
T h e F u t u r e o f i n n o v a t i o n i s
Pat Pataranutaporn
Future
Present
Past
Research Entrepreneurship Liberal Arts
Knowledges?
Music by Justin Timberlake