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Night session
Pat Pataranutaporn (P.P.) JSTP #12
MISSION IMPOSSIBLE
Night session
Pat Pataranutaporn (P.P.) JSTP #12
MISSION IMPOSSIBLE
โครงงานของฉันและ
ประสบการณ์
Present งานต่าง
แดน
IMPOSSIBLEMISSION
IMPOSSIBLEMISSION
IMPOSSIBLE PROJECT
Family
JSTP
??
?
?
?
? ?
?
?
?
?
? ?
?
?
?
?
?
?
?
?
?
?
? ?
“ Everyone has a question.
What's yours? “
“ Everyone has a question.
What's yours? “
“ Never be afraid to asked “
ZOMBI
E
Dr.Atichart Ketsuwan
and his reseach that
use mathematics to
study the history of
ancient temple
วัดอุ
โมง,เชียงให
ม่
NatureThe hidden treasure
Biomimicry
“ Never be afraid to asked “
IMPOSSIBLE
IMPOSSIBLE
IS NOTHING
IMPOSSIBLE
IMPOSSIBLE
IMPOSSIBLE
Right
solution
POLYSTYRENE
Styromelt™,http://www.polystyrenerecycle.com/
100,000 Tons
Styromelt™,http://www.polystyrenerecycle.com/
of Polystyrene was produce in UK.
“Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal...
“Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal...
“Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal...
“Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal...
Information & Communication Technology Center, 
Ministry of Energy, THAILAND
Natural degradation
>500 years
Information & Communication Technology Center, 
Ministry of Energy, THAILAND
Natural degradation
>500 years
My inspiration
My inspiration
My inspiration
My inspiration
My inspiration
My inspiration
สิ่งเล็กๆที่เรียกว่า
แบคทีเรีย
สิ่งเล็กๆที่เรียกว่า
แบคทีเรีย
??
?
?
?
? ?
?
?
?
?
? ?
?
?
?
?
?
?
?
?
?
?
? ?
WHYPS foam
spend long time in
degradation ?
chemical
formula is (C8H8)n
Polymer of styrene
Hydrogen
Carbon
organic
compound
s
organic
compound
s
organic
compound
s
Naturally
POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally
POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally
POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally
POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally
HOW we isolate the
bacteria that able to
degrade foam ?
If bacteria can
degrade foam,
how it utilize ?
chemical
formula is (C8H8)n
Polymer of styrene
Hydrogen
Carbon
Bacteria nutritional requirements
‣ Energy source
‣ Carbon source
‣ Nitrogen source
‣ Minerals
‣ Water
‣ Growth factors
HOW we isolate the
bacteria that able to
degrade foam ?
POLYSTYRENE
Isolation of polystyrene foam biodegradable
bacteria from petrol contaminated soil
Researcher : Pat Pataranutaporn Advisor...
egraded flasks, 19 isolates were bacilli
02
of plastic produced from styrene. It is a
material with exceptional insulation...
egraded flasks, 19 isolates were bacilli
02
of plastic produced from styrene. It is a
material with exceptional insulation...
The results showed that bacteria from petrol contaminated soil could
grow in mineral medium that polystyrene foam was the ...
Howto make sure that
bacteria that was isolate can
survive in our condition ?
Molecular biology technic
Molecular biology technic
time(week)
Molecular biology technic
time(week)
Microbe diversity
Molecular biology technic
time(week)
Microbe diversity
Dominant species
ControlTreatment
100x 200x 500x
Polystyrene in Medium with Soil sample
regular polystyrene foam that didn’t use in experiment.
Magnification = 300X
Control Experimental group
WHYPS foam spend long time in degradation ?
HOW TO FIND THE MICROBE THAT ABLE TO DEGRADE PS FOAM ?
HOWwe isolate the bacte...
??
?
?
?
? ?
?
?
?
?
? ?
?
?
?
?
?
?
?
?
?
?
? ?
Question
Question
No great discovery was ever
made without a bold guess
 Isaac Newton
Start
Goal
Start
Goal
Why
Why
Why
Start
Goal
Why
Why
Why
Why
Why
Why
Start
Goal
Why
Why
Why
Why
Why
Why
osamu shimomura
Wins Nobel Prize in Chemistry 2008
for Discovery Of Green Fluorescent Protein
??
?
?
?
? ?
?
?
?
?
? ?
?
?
?
?
?
?
?
?
?
?
? ?
You have a chance
to answer your
own question .
“ Never be afraid to asked “
POSSIBLEIM
AKE POSSIBLEI M
THANK YOU
Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol(
(Current(advisor)(
Miss(Apinya(Boonkhum( Mrs.RaCa...
Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol(
(Current(advisor)(
Miss(Apinya(Boonkhum( Mrs.RaCa...
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
Mission impossible
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Mission impossible

  1. 1. Night session Pat Pataranutaporn (P.P.) JSTP #12 MISSION IMPOSSIBLE
  2. 2. Night session Pat Pataranutaporn (P.P.) JSTP #12 MISSION IMPOSSIBLE
  3. 3. โครงงานของฉันและ ประสบการณ์ Present งานต่าง แดน
  4. 4. IMPOSSIBLEMISSION
  5. 5. IMPOSSIBLEMISSION
  6. 6. IMPOSSIBLE PROJECT
  7. 7. Family JSTP
  8. 8. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
  9. 9. “ Everyone has a question. What's yours? “
  10. 10. “ Everyone has a question. What's yours? “ “ Never be afraid to asked “
  11. 11. ZOMBI E
  12. 12. Dr.Atichart Ketsuwan and his reseach that use mathematics to study the history of ancient temple วัดอุ โมง,เชียงให ม่
  13. 13. NatureThe hidden treasure
  14. 14. Biomimicry
  15. 15. “ Never be afraid to asked “
  16. 16. IMPOSSIBLE
  17. 17. IMPOSSIBLE IS NOTHING
  18. 18. IMPOSSIBLE
  19. 19. IMPOSSIBLE
  20. 20. IMPOSSIBLE Right solution
  21. 21. POLYSTYRENE
  22. 22. Styromelt™,http://www.polystyrenerecycle.com/
  23. 23. 100,000 Tons Styromelt™,http://www.polystyrenerecycle.com/ of Polystyrene was produce in UK.
  24. 24. “Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California. "Ease of Disposal". Retrieved 2009-06-25
  25. 25. “Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California. "Ease of Disposal". Retrieved 2009-06-25 CARCINOGEN Styrene Monomer Carbon Monoxide
  26. 26. “Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California. "Ease of Disposal". Retrieved 2009-06-25 CARCINOGEN Styrene Monomer Carbon Monoxide
  27. 27. “Polystyrene Fact Sheet,” Foundation for Advancements in Science and Education, Los Angeles, California. "Ease of Disposal". Retrieved 2009-06-25 CARCINOGEN Styrene Monomer Carbon Monoxide 1%RECY CLE
  28. 28. Information & Communication Technology Center,  Ministry of Energy, THAILAND Natural degradation >500 years
  29. 29. Information & Communication Technology Center,  Ministry of Energy, THAILAND Natural degradation >500 years
  30. 30. My inspiration
  31. 31. My inspiration
  32. 32. My inspiration
  33. 33. My inspiration
  34. 34. My inspiration
  35. 35. My inspiration
  36. 36. สิ่งเล็กๆที่เรียกว่า แบคทีเรีย
  37. 37. สิ่งเล็กๆที่เรียกว่า แบคทีเรีย
  38. 38. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
  39. 39. WHYPS foam spend long time in degradation ?
  40. 40. chemical formula is (C8H8)n Polymer of styrene Hydrogen Carbon
  41. 41. organic compound s organic compound s organic compound s Naturally
  42. 42. POLYSTYRENE organic compound s organic compound s organic compound s Naturally
  43. 43. POLYSTYRENE organic compound s organic compound s organic compound s Naturally
  44. 44. POLYSTYRENE organic compound s organic compound s organic compound s Naturally
  45. 45. POLYSTYRENE organic compound s organic compound s organic compound s Naturally
  46. 46. HOW we isolate the bacteria that able to degrade foam ?
  47. 47. If bacteria can degrade foam, how it utilize ?
  48. 48. chemical formula is (C8H8)n Polymer of styrene Hydrogen Carbon
  49. 49. Bacteria nutritional requirements ‣ Energy source ‣ Carbon source ‣ Nitrogen source ‣ Minerals ‣ Water ‣ Growth factors
  50. 50. HOW we isolate the bacteria that able to degrade foam ?
  51. 51. POLYSTYRENE
  52. 52. Isolation of polystyrene foam biodegradable bacteria from petrol contaminated soil Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang Abstract 01 The purpose of this study was to isolate polystyrene foam biodegradable bacteria from petrol contaminated soil. Mineral salt medium with polystyrene foam as the only carbon source was used in this experiment. After 4 months of incubation, surface of polystyrene foam was observed compared with the control group. The result showed that there were some traces of degradation on the surface of polystyrene foam when compared to the control group which the same source of soil was used as inoculum but had been sterilized before inoculation. Twenty-four isolates of Gram-positive bacteria were isolated from polystyrene foam degraded flasks, 19 isolates were bacilli and 5 were cocci shape. Introduction 02 Polystyrene foam is a type of plastic produced from styrene. It is a lightweight, moisture-resistant material with exceptional insulation properties. Products made from foamed polystyrene are nearly ubiquitous, for example packing materials, insulation, and foam drink cups. Polystyrene takes hundreds of years to decompose and is often abundant as a form of pollution in the outdoor environment. Methodology 03 Methodology. Soil samples were collected from sources that were contaminated by petrol. One gram of contaminated soil sample was inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4, 0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg; NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask. Small pieces of 0.1 g of polystyrene foam were added as carbon source. Prior to use, polystyrene foam was soaked in 70% alcohol and washed two times with distilled water and dried in desiccator for two days. In the control group, 1 gram of sterilized petrol contaminated soil sample was used. All experiment was carried out in triplicate. All flasks were incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of incubation 1 ml of cell suspension was inoculated into a new flask and incubated in the same condition for 2 months. Surface of polystyrene foam was observed under stereo zoom microscope and taken photograph. Culture broth was diluted and spread onto glucose yeast extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria were picked, purified and morphological studied by Gram-staining and observed under microscope. Result and discussion 04 The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape. Furthermore, some traces of degradation were found on the surface of polystyrene foam from the experiment flasks rather than the control group. These results indicated that there are polystyrene foam degradable bacteria from petrol contaminated soil and It might use polystyrene foam as carbon and energy sources. However, further investigation need to be done for confirmation the results of these experiments and that is our future study. Acknowledgement: This project was supported by JSTP. National Science and Technology Development Agency. Experimental group Control group Reference 05 Daniel Burd (2008) Plastic not fantastic. Libraries (online). Available : http://www.science.uwaterloo.ca Rod shape Positive gram Round shape Positive gram 79.1% (19 isolates ) 20.9% (5 isolates ) Types of bacteria that isolated from the experimental group The surface of polystyrene foam
  53. 53. egraded flasks, 19 isolates were bacilli 02 of plastic produced from styrene. It is a material with exceptional insulation rom foamed polystyrene are nearly g materials, insulation, and foam drink ds of years to decompose and is often n the outdoor environment. gy 03 s were collected from sources that were gram of contaminated soil sample was ral salt medium (K2HPO4, 2.0g; KH2PO4, 05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; H2O, 0.012mg; CuCl2.2H2O, 0.001mg; 2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, distilled water, pH 7.3) in 250 ml flask. ene foam were added as carbon source. was soaked in 70% alcohol and washed and dried in desiccator for two days. In erilized petrol contaminated soil sample carried out in triplicate. All flasks were , 30oC for 2 months. After 2 months of on was inoculated into a new flask and be done for confirmation the results of these experiments and that is our future study. Experimental group Control group Rod shape Positive gram Round shape Positive gram 79.1% (19 isolates ) 20.9% (5 isolates ) Types of bacteria that isolated from the experimental group The surface of polystyrene foam
  54. 54. egraded flasks, 19 isolates were bacilli 02 of plastic produced from styrene. It is a material with exceptional insulation rom foamed polystyrene are nearly g materials, insulation, and foam drink ds of years to decompose and is often n the outdoor environment. gy 03 s were collected from sources that were gram of contaminated soil sample was ral salt medium (K2HPO4, 2.0g; KH2PO4, 05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg; H2O, 0.012mg; CuCl2.2H2O, 0.001mg; 2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg, distilled water, pH 7.3) in 250 ml flask. ene foam were added as carbon source. was soaked in 70% alcohol and washed and dried in desiccator for two days. In erilized petrol contaminated soil sample carried out in triplicate. All flasks were , 30oC for 2 months. After 2 months of on was inoculated into a new flask and be done for confirmation the results of these experiments and that is our future study. Experimental group Control group Rod shape Positive gram Round shape Positive gram 79.1% (19 isolates ) 20.9% (5 isolates ) Types of bacteria that isolated from the experimental group The surface of polystyrene foam Experimental group control group
  55. 55. The results showed that bacteria from petrol contaminated soil could grow in mineral medium that polystyrene foam was the only carbon source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates were rod and 5 isolates were round shape.
  56. 56. Howto make sure that bacteria that was isolate can survive in our condition ?
  57. 57. Molecular biology technic
  58. 58. Molecular biology technic time(week)
  59. 59. Molecular biology technic time(week) Microbe diversity
  60. 60. Molecular biology technic time(week) Microbe diversity Dominant species
  61. 61. ControlTreatment
  62. 62. 100x 200x 500x Polystyrene in Medium with Soil sample regular polystyrene foam that didn’t use in experiment.
  63. 63. Magnification = 300X Control Experimental group
  64. 64. WHYPS foam spend long time in degradation ? HOW TO FIND THE MICROBE THAT ABLE TO DEGRADE PS FOAM ? HOWwe isolate the bacteria that able to degrade foam ? If bacteria can degrade foam, how it utilize ?
  65. 65. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
  66. 66. Question
  67. 67. Question
  68. 68. No great discovery was ever made without a bold guess  Isaac Newton
  69. 69. Start Goal
  70. 70. Start Goal Why Why Why
  71. 71. Start Goal Why Why Why Why Why Why
  72. 72. Start Goal Why Why Why Why Why Why
  73. 73. osamu shimomura Wins Nobel Prize in Chemistry 2008 for Discovery Of Green Fluorescent Protein
  74. 74. ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
  75. 75. You have a chance to answer your own question .
  76. 76. “ Never be afraid to asked “
  77. 77. POSSIBLEIM
  78. 78. AKE POSSIBLEI M
  79. 79. THANK YOU
  80. 80. Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol( (Current(advisor)( Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang(
  81. 81. Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol( (Current(advisor)( Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang( Advisor

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