Mission impossible II

1. The mission ImpossibleII JSTP 12

2. The mission ImpossibleII JSTP 12

3. The mission Impossible II The toy story

4. Impossible The mission

5. Impossible Project

8. โครง

9. โครง

10. โครงโครงงาน

11. โครงโครงงาน

12. โครงโครงงาน Project

13. PROJECT = TOY GAME FUN

14. PROJECT = TOY GAME FUN

15. แบบฟอร์ม โครงงาน ที่มาและความสำคัญ วัตถุประสงค์ประโยชน์ที่คาดว่าจะได้รับตัวแปรที่เกี่ยวข้อง

16. คู่มือการเล่น เล่นอย่างไรให้สนุก

17. คู่มือการเล่น เล่นอย่างไรให้สนุก อ่านจบแล้ว จะสนุกไหม

20. Every years someone has a great speech....

28. @#!$#%wee-tah-kah loo-loo

29. คันหูจุงบ่องตง

31. ต่อยอด

32. ต่อยอด สร้างยาลดอาการ คันหูของFurby

34. Fin~

37. Innovation

40. What did we play ?

41. Detective What did we play ?

42. Detective Created What did we play ?

43. Detective Created Divas What did we play ?

44. Detective Created Divas Action What did we play ?

45. Detective Created Divas Business Action What did we play ?

46. Detective Created Divas Impersonate Business Action What did we play ?

47. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา

48. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์

49. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว

50. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว เล่นเกม แปลงร่าง

51. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว เกมเศรษฐี เล่นขายของ เล่นเกม แปลงร่าง

52. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว บทบาทสมมุติ หมอรักษาคนไข้ เกมเศรษฐี เล่นขายของ เล่นเกม แปลงร่าง

53. P.P. TOYs

54. P.P. TOYs

55. P.P. TOYs

56. P.P. TOYs

57. P.P. TOYs

58. P.P. TOYs

60. Bacteria

61. Bacteria Styrofoam

62. Biodegradation Bacteria Styrofoam

63. My project in academic style.

65. Styrofoam

66. Styrofoam Styrene monomer

68. Polystyrene Chemical formula is (C8H8)n Monomer styrene Thermoplastic Blowing agents

69. Polystyrene Chemical formula is (C8H8)n Monomer styrene Thermoplastic Blowing agents 500 years++ for degradation http://en.wikipedia.org/wiki/Polystyrene

71. Recycling & Incineration

72. Recycling & Incineration 1% turn back to polystyrene most of the polystyrene is converted into carbon dioxide, water vapor, and heat. "Ease of Disposal". Retrieved 2009-06-25

73. Photo by Napat Jaitui JSTP # 15

74. Styrene Monomer Carbon Monoxide Photo by Napat Jaitui JSTP # 15

75. Styrene Monomer Carbon Monoxide Photo by Napat Jaitui JSTP # 15 CARCINOGEN

77. c c c c c c c c

78. c c c c c c c c Bacteria nutrition ‣ Energy source ‣ Carbon source ‣ Nitrogen source ‣ Minerals ‣ Water ‣ Growth factors

79. Howcan I find the bacteria that able to degrade a styrofoam?

81. SOIL

82. SOIL

83. SOIL Landfill Landfill foam

89. The ability

90. The key to isolate the effective microbe is the degradability.

91. MSM Broth Bacteria selective broth

93. MSM broth ‣K2HPO4 ‣KH2PO4 ‣(NH4)2SO4 ‣MgSO4 ‣ FeSO4.2HO2 ‣MnCl2.4H2O ‣CoCl2.6H2O ‣CuCl2.2H2O ‣NiCl2.6H2O ‣Na2MoO4.2H2O ‣ZnSO4.7H2O ‣H3BO3 Sterile Styrofoam MSM Broth Bacteria selective broth

96. Shake in shaker for 1 month then inoculate to new fresh broth for sub culture.

97. Every week the solution in each flask was taken to the eppendorf then stored at 2C๐ for stop bacteria growth that use for monitor the changing of bacteria population.

102. MSM broth ‣K2HPO4 ‣KH2PO4 ‣(NH4)2SO4 ‣MgSO4 ‣ FeSO4.2HO2 ‣MnCl2.4H2O ‣CoCl2.6H2O ‣CuCl2.2H2O ‣NiCl2.6H2O ‣Na2MoO4.2H2O ‣ZnSO4.7H2O ‣H3BO3 Sterile Styrofoam MSM Broth Bacteria selective broth

106. MSM Broth Bacteria selective broth Foam in landfill Soil in landfill

111. Week

112. Week

113. Week Dominant species

114. Week Dominant species Diversity

115. Every week the solution in each flask was taken to the eppendorf then stored at 2C๐ for stop bacteria growth that use for monitor the changing of bacteria population.

116. DNA Replication : PCR (TopTaq Master Mix Kit) 16S rRNA gene Amplification by using Primer VR (Medlin et al., 1998) & VFC (Muyzer et al., 1993)

119. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine

120. DGGE (Denature Gradient Gel Electrophoresis) - 30% Urea + Formamine 60% Urea + Formamine +

124. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine GC rich

125. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine GC low GC rich

126. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine GC low GC rich

127. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)

133. The microscopic structure of Polystyrene observed by SEM Pat Pataranutaporn1 , Apinya.J2 Assistant prof. Savaporn Supaphol3 , 1 PSU.Wittayanusorn school, 2 Scientific Equipment Center Prince of Songkla University 3 Kasetsart University

134. Control : Polystyrene in MSM broth without bacterial source. Regular polystyrene foam that didn’t use in experiment. 100x 200x 500x The comparison of Polystyrene microscopic structure Control

135. Regular polystyrene that didn’t use in experiment. Control

136. Polystyrene in Medium with bacteria from foam sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment. Polystyrene in Medium with bacteria from foam sample. Bacteria from foam

137. Polystyrene in Medium with bacteria from foam sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment. Bacteria from foam

138. Polystyrene in Medium with bacteria from soil sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment. Bacteria from soil

139. Polystyrene in Medium with bacteria from soil sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment.Polystyrene in Medium with bacteria from soil sample. Bacteria from soil

142. Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Foam sampleSoil sampleControl

143. Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Foam sampleSoil sampleControl

146. Molecular cloning DNA isolation & DNA Analyzing Pat Pataranutaporn1 , Sureeporn Nualkaew2 , Prof. Dr. Amornrat Phongdara2 1 PSU.Wittayanusorn school, 2 Prince of Songkla University

149. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis) Hello Who are you ?

153. Molecular cloning Despicable Me: Minion Mayhem

154. PCR Product

155. PCR Product

156. PCR Product Mix DNA for ligation pGEM- T easy Target DNA

157. PCR Product Mix DNA for ligation

158. PCR Product Mix DNA for ligation

159. Transform plasmid to the competent cell.

160. E.Coli as Transform plasmid to the competent cell.

164. Transform plasmid to the competent cell. The separate DNA

166. Electropherogram Code name : Control 4

167. Code name : Control 4 Basic Local Alignment Search Tool (BLAST) Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea Description 16S ribosomal RNA sequences (Bacteria and Archaea) Program BLASTN 2.2.27+ Molecule type nucleic acid Query Length 1055

169. Code name : Control 4 Phylogenetic tree

172. Electropherogram Code name : Control 7

178. Code name : Fe5

179. Bacterial plating Plate streaking & Plate spreading Pat Pataranutaporn1 , Panwong Kuntanawat2 1 PSU.Wittayanusorn school, 2 King Mongkut's University of Technology Thonburi

180. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr.

182. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr. Polystyrene-coacrylic acid (PSA) (particles diameter 500 nm) MSM Agar

183. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr. Polystyrene-coacrylic acid (PSA) (particles diameter 500 nm) MSM Agar Polysaccharide Cx(H2O)y

193. Research Achievements

194. Research Achievements

195. Research Achievements 13th NCSC, Jaipur India 2011 Youth summit 2012, Dubai UAE JSTP Scholarship STT 36 BYEE, Leverkuzen Germany BYEE Poster Prize from india

196. Publication & media

198. JSTPMedia www.jstpmedia.org

199. Biodegradation of Polystyrene Foam by the Microorganisms from Landfill

200. P.P. TOYs

201. P.P. TOYs

203. หนังตะลุง

204. หนังตะลุง WEB app

205. หนังตะลุง WEB app Culture

209. Developed in SWF format http://jstpmedia.org/images/complete.swf

211. A S ◀ ◀ ◀ ◀ move to the next character Head(Speak) Left hand right hand Body ﬂip

212. Demo

213. JSTP 15/3

222. P.P. TOYs

223. P.P. TOYs

225. Space

226. Space Gravity

227. Space Enzyme Gravity

228. JAXA

229. v

230. v

231. The Evaluation of Amylase Activity under Microgravity By JSTP 12 Pat Pataranutaporn Pondet Ananchai Arnan Schuett

232. ChemistryBiotechnology Electronic Research Team JSTP 12

233. ChemistryBiotechnology Electronic Research Team JSTP 12 The youngest team

234. Possibility of Enzyme

235. Possibility of Enzyme Collision Theory

236. Possibility of Enzyme Collision Theory ‣Enzyme Concentration ‣Substrate Concentration ‣Inhibitors ‣Temperature ‣pH

238. Gravity

239. GravityFree motion of molecule

243. Salivary stress markers and psychological stress in simulated microgravity: 21 days in 6° head-down tilt. Rai B, Kaur J. Catholic University Leuven, Belgium. Spaceflight occurs in an environment of temperature extremes, microgravity, solar and galactic cosmic radiation, lack of atmospheric pressure, and high-speed micrometeorites. Exposure to microgravity and the space environment during space missions of short and long duration has important medical and health implications for astronauts. Psychological well-being is of increasing importance in planned spaceflights and interplanetary missions of long duration. The 6° head-down tilt (HDT) is an established method of mimicking low gravity on earth. The aim of the present study was to determine the effects of 21 days of HDT on psychological stress in 12 healthy male volunteers. Psychological state was assessed by the current stress test, and chromogranin-A (CgA), cortisol, alpha-amylase, and beta-endorphin were measured in saliva. After one week of HDT, all volunteers developed psychological stress, and secretion of CgA, cortisol, alpha-amylase, and beta-endorphin were all significantly higher. Thus, 6° HDT appears to be a valid model to induce psychological stress changes in the immune system, changes that might also be encountered by astronauts and cosmonauts during both a short stay in space, such as that required while orbiting a space station, and in longer spaceflights. Adaptation mechanism

244. Difference enzyme activity rate affected by gravity

248. Enzyme containing

249. Enzyme containing Pumping system

250. Enzyme containing Pumping system Starch measuring

251. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring

252. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring

253. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2

254. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 24 2

255. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 246 2 24

256. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 24 46

257. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 2 04 46

260. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 2 04 46 low starch remain High starch remain

261. 026 48

262. 026 48

263. starch remain (%v/w) 026 48

264. starch remain (%v/w) Time (s) 026 48

268. starch remain (%v/w) Time (s) 026 48 Y

269. starch remain (%v/w) Time (s) 026 48 Y X

270. starch remain (%v/w) Time (s) 026 48 Y X Rate =

272. Working period

273. Working period 2 moths for discussing

274. Working period 2 moths for discussing 3 hours for proposal

275. Working period 2 moths for discussing 3 hours for proposal 1 night for presentation

276. Working period 2 moths for discussing 3 hours for proposal 1 night for presentation 5 minutes for presentation rehearsing

278. JSTP Team JSTP Team

280. ไม่มี limit ชีวิตเกินร้อย

281. PP’s TOYP.P. TOYs

282. PP’s TOYP.P. TOYs

283. What you are seeing now.

284. Presentation DESCIPost modern interface

285. Available at JSTPMedia soon !

289. !

290. You have a chance to answer your own question .

291. ของเล่นที่สนุก เราต้องเลือกเอง

292. “ Never be afraid to asked “

293. POSSIBLEIM

294. AKE POSSIBLEI M

295. Right now...

296. It’s your turn

297. It’s your turn

298. THANK YOU

Mission impossible II

Recommended

Recommended

More Related Content

Similar to Mission impossible II

Similar to Mission impossible II (12)

More from Pat Pataranutaporn

More from Pat Pataranutaporn (20)

Recently uploaded

Recently uploaded (20)

Mission impossible II