Thank you 1. What is responsible for anterior:posterior axis formation in the chicken? How is dorsal ventral patterning accomplished? 2. What is the role of the Posterior Marginal zone in early chicken development? 3. What is the primitive streak and why is it important in chicken development? 4. What is the function of Henson\'s Node? To what is it analogous in amphibian development? Solution 1.The primitive streak defines the axes of the embryo. It extends from posterior to anterior; migrating cells enter through its dorsal side and move to its ventral side; and it separates the left portion of the embryo from the right. Those elements close to the streak will be the medial (central) structures, while those farther from it will be the distal (lateral) structures 2.Primitive streak is induced by the posterior marginal zone (PMZ) of Koller\'s sickle, which can also induce Hensen\'s node. If cell movement in the PMZ is blocked, primitive streak does not form.Thus, the PMZ acts as an organizer.Cells in marginal zones of the embryo, like the PMZ, are key to development and cell fate determination in chick embryos. 3.The primitive streak is a structure that forms in the blastula during the early stages of avian, reptilian and mammalian embryonic development. It forms on the dorsal (back) face of the developing embryo, toward the caudal or posterior end.The presence of the primitive streak will establish bilateral symmetry, determine the site of gastrulation and initiate germ layer formation. To form the streak, reptiles, birds and mammals arrange mesenchymal cells along the prospective midline, establishing the second embryonic axis, as well as the place where cells will ingress and migrate during the process of gastrulation and germ layer formation.The primitive streak extends through this midline and creates the left–right and cranial–caudal body axes,and marks the beginning of gastrulation.This process involves the ingression of mesoderm progenitors and their migration to their ultimate position,where they will differentiate into the mesoderm germ layer that, together with endoderm and ectoderm germ layers, will give rise to all the tissues of the adult organism. 4. The primitive knot (or primitive node) is the organizer for gastrulation in the vertebrate embryo. Diversity In birds, it is known as \"Hensen\'s node\", and is named after its discoverer Victor Hensen. In amphibians, it is known as \"Spemann\'s organizer\", and is named after Hans Spemann In chick development, the primitive knot starts as a regional knot of cells that forms on the blastodisc immediately anterior to where the outer layer of cells will begin to migrate inwards - an area known as the primitive streak, which is involved with Koller\'s sickle. Posterior to the node is the primitive pit, where the cells of the epiblast (the upper layer of embryonic cells) initially begin to invaginate. This invagination expands posteriorly into the primitive groove as the cells layers c.