Stable for 9 months
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This document discusses the stability of recombinant HLA Class II antigens expressed from transformed cell lines over a period of nine months of continuous culture. The expression levels remained stable for cell lines expressing DRB1*0101 and DRB1*1001 antigens grown both with and without antibiotics in the culture medium. The percentage of positive cells expressing the antigens remained high, around 80-100%, at three, six, and nine months.
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La biotecnología es la disciplina que integra las ciencias de la vida y las ciencias de la ingeniería para obtener productos mediante sistemas biológicos como microorganismos, células, plantas y animales. Los primeros organismos utilizados fueron microorganismos como bacterias y hongos, aunque posteriormente se emplearon también plantas y animales. La biotecnología moderna utiliza organismos modificados genéticamente para obtener mayores beneficios.
"What is Different This Time Around" at SaaStr Annual 2016
Mark Suster shares his thoughts on the change in funding climate in 2016, what is the same this time around, and what most certainly isn't at SaaStr Annual 2016 held in San Francisco Feb 9-11th. www.saastrannual.com
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"What is Different This Time Around" at SaaStr Annual 2016
"What is Different This Time Around" at SaaStr Annual 2016
Stable for 9 months
- 1. 1 All Content © 2014 Immucor, Inc. Expression of Recombinant HLA Class II Antigens from transformed cell lines remains stable for at least nine months of continuous culture 0 20 40 60 80 100 120 DRB1*0101-antibiotic medium DRB1*0101-no antibiotic medium DRB1*1001-antibiotic medium DRB1*1001-no antibiotic medium BC10PositiveCells (%) 3 month 6 month 9 month
Editor's Notes
- Here is shown that the expression of recombinant HLA Class II antigens from transformed cell lines remains stable for at least nine months of continuous culture, the conclusions were confirmed by both Luminex based assay and flow-cytometry.