Bioltinting

1. 3D BIO-PRINTING
TECHNOLOGY
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2. 3D BIO-PRINTING is the three-dimensional printing of
biological tissue and organs through the layering of living
cells.
1. ORGAN TRANSPLANTATION
It refers to transplantation of organs due to organ failure or
injury.
2. TISSUE ENGINEERING
It is the study of the growth of new connective tissue, or
organs, from cells.
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3. • Organ failure is a worldwide problem, and its only
treatment is organ transplantation or tissue
replacement
ORGAN TRANSPLANTATION
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4. • Focuses on the association of living cells with
signaling molecules and supports, known as scaffolds.
Importance of scaffolds
1. Substrate attachment is required for cell growth and
proliferation.
TISSUE ENGINEERING
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5. 2. Tissue construct must have organ specific shape, the shape of the
construct will influence the cell behavior;
3. The scaffold serves not only as an attachment substrate, but also
as a source of inductive signals for cell differentiation, migration,
proliferation and orientation;
4. The mechanical properties provided initially by the scaffold will
be maintained.
Contd…
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6. Applications of 3D printing
(Our Focus: Bioprinting)
Organs Medical Applications
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7. • BIO PRINTING is an automated computer aided
layer-by-layer deposition of biological materials for
manufacturing of functional human organs/constructs.
BIO-PRINTING
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8. BEST QUALITIES OF THIS TECHNIQUES:
RAPID
PROTOTYPING
HIGH
RESOLUTION
HIGH PRECISION
COMPUTER
CONTROL
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9. 6
What 3D Bioprinting Is and How It Works - Bing video

10. Inkjet-BASED
Cheapest technology
 In this method, the bio-ink is stored in a cartridge. These
chambers are very small and have a controlled actuator
(piezoelectric or heating element) that projects the bio-ink onto
the substrate.
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11. EXTRUSION-BASED
 Reduced amounts of shear stress.
The bio-ink rests at the cylindrical deposit waiting for the
pneumatic or mechanical pressure, as pulse or continued, from
a piston which propels the biomaterial through a nozzle onto
the substrate.
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12. LASER-BASED
 Uses laser assisted technology to project the ink droplets onto
the substrate.
 Laser pulses trigger when hits the laser absorbing layer, the area
where the laser hit it evaporates, and the generated high gas
pressure propels the biomaterial onto the substrate.
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13. Stereolithography
 Stereolithography (SLA or SL; also known as vat photopolymerization,
or resin printing) is a form of 3D printing technology used for creating
models, prototypes, patterns, and production parts in a layer-by-layer
fashion using photochemical processes by which light causes chemical
monomers and oligomers to cross-link together to form polymers.
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14. https://www.onlinelibrary.wiley.com/doi/10.1002/smll.201805510

15. ADVANTAGES
 Replace human tissue
by full body transplant.
 Allows scientists to
eliminate the wait list of
organ transplants ·
 Higher survival rate of
printed cells.
 Offers high precise
resolution
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16. DISADVANTAGES
 Organ is not sure about
whether they can fit into a
human body .
 Printing capabilities of
complicated tissues.
 Can be very costly depending
on the technique.
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17. CONCLUSION
With the continuous growth of the world’s population, and
increase of human life expectancy, more cases of organ failure
and tissue damage appear.
Most common bio-printing methods were described and
discussed with their characteristics and limitations.
In terms of future perspectives for this work, more bio print
testing would be needed to be done to optimize the bio ink,
substrate and the process parameters.
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