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Sigma D Additional Data
1. Sigma DTranscription Factor
Non-chaining cells are typically found as single or
double cells, are motile, and the σD transcription
factor is turned on
Chaining cells are typically found as long chains of
cells, are sessile (non-motile), and the σD
transcription factor is off
3. Sigma DTranscription Factor
The microscopy results show that 3610 and 3610
cured generally do not form chaining cells,
whereas 168 forms some chaining cells and some
non-chaining cells, and PY79 forms almost
exclusively chaining cells.
These results suggest that 3610, 3610 cured, and
possibly 168 have the σD transcription factor on,
whereas PY79 does not.
4. Sigma DTranscription Factor
Although research on the σD transcription factor
has not directly linked it to transcription of the yuk
operon, it may play a role in the transcription of
another secretion system in 3610 (and to a lesser
extent in 168) that is non-functional in PY79 and
may be accounting for the observed differences in
secretion.
Comparison of the regions regulated by σD in 168
and PY79 may be the first step in the analysis of
genetic differences between these two strains.
5. Sigma BTranscription Factor
In addition, the σB transcription factor may be
another transcription factor to consider in future
research because it has been implicated in the
stress response of Bacillus subtilis.As a homolog
to M. tuberculosis’ virulence factor, ESXA,YukE’s
secretion may be significantly affected by the
cell’s stress response.
Editor's Notes
Transcription factor might be contributing to production of YukE and/or something else causing YukE to get out of the cell
Maybe this is because it’s necessary to secrete YukE (or its equivalent in pathogenic bacteria) when the cells are on their own – i.e. necessary for survival, but not necessary when they form long chains of cells?
Having sigD on also indicates that swrA, the gene for swarming is transcribed (3610 swarms, PY79 does not)
