: Clinical Laboratory
semester II
CMT 04211
BY JACOB NOAH LAB SCIENTIST, BSC IN HEALTH LAB SCIENCE, ACADEMIC TUTOR II
,RESEARCH OFFICER
TAIFA INSTITUTE OF HEALTH AND ALLIED SCIENCES
4/9/2024
1
LEARNING OBJECTIVES
 At the end of this session each student is expected to be able
to;-
 Define IPC, health care acquired infections and Standard precaution
 Outline objectives of IPC
 Outline four major types of health care acquired infections
 Explain risk factors for health care acquired infections
 Explain components of standard precautions in IPC (consider every person, hand
hygiene, personal protective equipment, handle sharps, safe dispose of wastes
and process instruments)
4/9/2024
2
INTRODUCTION
 In healthcare settings, there is a need for a practical,
evidence-based approach for preventing patients and health
workers from being harmed by avoidable infections
 IPC is the aspect of health care which aims to ensure that
patients do not contract infections as a result of attending
health care facility for assessment, examination or treatment.
4/9/2024
3
DEFINITIONS
 HAIs, also termed nosocomial infections, are infections
occurring in a patient during the process of care in a
hospital or other health-care facility, which was not
present or incubating at the time of admission. HCWs
are also at risk for developing HAIs.
 IPC programs are implemented to prevent HAIs from
occurring and spreading within health facilities.
4/9/2024
4
 Standard precaution, are a simple set of effective
practice guidelines (creating a physical, mechanical
or chemical barrier) to protect health service
provider and clients/patients from infection with a
range of pathogens including blood borne.
4/9/2024
5
 Infection is the successful entrance into the body by
pathogenic microorganisms and the reaction of the body to
the presence of these microorganisms.
 OR Infection is the entry and development of many parasites
or multiplication of an infectious agent in the body of
humans or other animals.
 Prevention is behaviors designed to avoid disease
4/9/2024
6
 Standard precaution, are a simple set of
effective practice guidelines (creating a
physical, mechanical or chemical barrier) to
protect health service provider and
clients/patients from infection with a range of
of pathogens including blood borne
4/9/2024
7
Objectives of Infection Prevention and Control
(IPC) in Health care Facility
 To protect patients/ clients from nosocomial
infections
 To protect health care workers from occupational
infections
 To protect communities from infectious diseases
 To prevent the environment from pollution
4/9/2024
8
Components of Standard Precautions in IPC
 Consider every person (patient or health workers) as potentially infectious and
susceptible to infection
 Consider dead bodies as also potentially infectious
 All items from all clients must be handled as if they are contaminated and all
clients treated as if they may be infected
 Use appropriate hand hygiene e.g. hand washing, Hand hygiene- this includes
hand washing and hand antisepsis, antiseptic and hand scrub.
 Wear personal protective equipment (PPE) including gloves, masks, goggles, caps,
gowns, boots and aprons.
4/9/2024
9
 Handle sharps, patient resuscitation and patient care equipment,
linen appropriately.
 Manage patient placement and patient environmental cleaning
appropriately
 Safe disposal of infectious waste materials to protect those who
handle them and prevent injury or spread to the community.
 Process instruments by decontamination, cleaning and then either
sterilize or HLD using recommended procedures
4/9/2024
10
CLASSIFICATIONS OF HAIs
 Endogenous infection. Many microorganisms that
cause HAIs come from the patient’s own body (the
term normal flora/endogenous flora can be used to
describe this).
 Eg; bacteria normally present in the colon can gain
entry to the urinary tract and cause urinary tract
infections.
4/9/2024
11
 Endogenous infection. Many microorganisms that
cause HAIs come from the patient’s own body (the
term normal flora/endogenous flora can be used to
describe this).
 Eg; bacteria normally present in the colon can gain
entry to the urinary tract and cause urinary tract
infections.
4/9/2024
12
CLASSIFICATIONS OF HAIs Cont…
 Exogenous infection. Result from the transfer of
microorganisms to the patient or HCW from an external
reservoir.
 Eg; microorganisms can be transferred through direct contact
with contaminated hands of HCWs and other patients (cross-
contamination), contaminated instruments and needles, or
the environment (exogenous flora).
4/9/2024
13
 Practicing hand hygiene at the right moment and other
elements of standard precautions can greatly reduce the
frequency of cross contamination between patients and
HCWs and thus reduce the incidence of infection. As
with endogenous infection, measures to protect a
persons natural resistance to infection can also help to
reduce the likelihood of infection if cross transmission
does occur.
4/9/2024
14
COMMON HAIs
 Urinary Tract Infection (UTI)
 Urinary tract infection is one of the most common HAIs.
Preventing UTI is a major factor in decreasing the overall
incidence of HAIs in HCFs. Healthcare-associated UTIs
are frequently related to urinary catheterization. Many
patients with a urinary catheter develop bacteriuria
(bacteria in the urine) because the catheter creates a
pathway for bacteria to enter the bladder.
4/9/2024
15
 However, it is important to make the distinction between
bacteriuria and an actual urinary tract infection. Patients
should not be considered to have a catheter related urinary
tract infection and should not receive antimicrobial treatment
solely because the urine is discoloured, has an odour, or
because the laboratory has cultured bacteria from the urine.
Unless the patient has clinical features of infection (e.g. fever,
rigors, other systemic features) they should not be
considered to have catheter related UTI.
4/9/2024
16
Factors that lead to UTI
 Factors that can lead to bacteriuria and may lead to UTIs include:-
 Urinary catheterization which creates a pathway that allows for endogenous
transfer of microorganisms (e.g. bacteria from the patient’s GI tract can be
transmitted to the urinary tract)
 Passage of organisms from the urine bag to the bladder (retrograde contamination)
can occur in patients with indwelling catheters
 Some microorganisms that can grow on the outside or inside of the catheter’s
tubing and in the urine itself
 Handling of the urinary catheter and urine bag by HCWs
4/9/2024
17
Reducing Hospital-Acquired Urinary Tract
Infections:
 Introducing an indwelling urinary catheter should be
done only when necessary and no other options are
effective. It is particularly important to limit the
duration of catheterization as much as possible
 Following appropriate procedures for inserting and
removing urinary catheters will also reduce the risk
of UTI
4/9/2024
18
B: Infections (SSI
 Surgical site infections is often the result of
contamination during the surgical procedure or
contamination of the surgical wound after the
procedure. SSIs are very common HAIs and often
require additional surgical procedures to treat the
infection.
4/9/2024
19
The following factors predispose a patient to
development of a SSI:
 Obesity
 Infection at another body site at the time of surgery
 Immunosuppression
 Malnutrition and anaemia
 Old age and chronic diseases such as diabetes and
malignancy
4/9/2024
20
Reducing SSIs in patients:
 Avoid prolonged preoperative hospitalization and recommend ambulatory surgery as often as
possible
 Avoid preoperative hair removal. If hair must be removed, clip it with scissors or electric
clippers just before the surgery. Do not shave using a razor blade (shaving has been attributed
to microscopic cuts in the skin that later serve as foci for bacterial multiplication)
 In the surgical room prepare a wide area around the proposed incision site with antiseptic
solution (2% alcohol chlorhexidine is generally appropriate)
 Practice good surgical techniques that minimize tissue trauma, control bleeding, eliminate
dead space, use minimal sutures, and maintain adequate blood supply and oxygenation
 Keep the duration of surgical procedures as short as possible. The rate of infection doubles
with each hour of surgery
4/9/2024
21
C. Health Care-Associated Pneumonia
 Healthcare associated pneumonia (HAP) is a
common HAI with a significant risk of a fatal
outcome. Most of these infections occur by
aspiration of bacteria growing in the back of the
throat or in the stomach. Pneumonia associated with
mechanical ventilation may be referred to as
ventilator associated pneumonia (VAP).
4/9/2024
22
 The range of microorganisms associated with
HAP/VAP is much wider than is the case for
community acquired pneumonia (CAP) and many of
these microorganisms are much more likely to be
resistant to antimicrobials. Therefore HAP/VAP may
be much harder to treat effectively with
antimicrobial agents than CAP.
4/9/2024
23
General activities employed in prevention and
control of diseases
 Standard precautions include:
 Hand Hygiene
 Personal Protective Equipment (PPE) appropriate for the level
of care being given or the potential infection risk associated
with an activity, even when there is no known risk of infection
 Respiratory hygiene and cough etiquette
 Injection and phlebotomy safety and sharps injury prevention
4/9/2024
24
General activities employed in prevention and
control of diseases
 Safe decontamination and sterilization of
medical equipment
 safe handling of Linen and laundry
 Environmental decontamination
 Healthcare waste management
4/9/2024
25
Risk Factors in Nosocomial Infection
 The longer the client is in a health care facility, the greater is
her/his risk of infection.
 Exposure to the facility environment changes the client own
normal body flora
 Risk factors that contribute to the development of
nosocomial infections can be grouped into three categories:
environment, therapeutic regimen, and resistance of the
client
4/9/2024
26
Environment
 Hospital, outpatient clinics, extended care facilities, the home, and schools
are reservoir of organisms that pose threat to the increasing number of
clients who have decreased resistance.
 The source of these organisms includes the air, other clients, families, and
visitors, contaminated equipment, food and personnel.
 Pneumonia and influenza can spread rapidly among clients and other
people in all types of facilities.
 Equipment that is not thoroughly cleaned, disinfected or sterilized can
spread many pathogens.
4/9/2024
27
Therapeutic regimen
 Multiple factors involved in therapies used to cure clients also can
contribute to the risk of infection.
 Drugs such as steroids, immunosuppressive agents, and cancer therapy,
as well as prolonged use of antibiotics predispose clients to infection.
 Equipment such as IV catheter, urinary catheters and feeding tubes that
invade orifices provides routes for bacterial invasion
 Inadequate dressing techniques for wound can provide media for
bacterial growth.
 Identifying treatments that pose risk and discontinuing their use as soon
as possible decrease the chance of nosocomial infection
4/9/2024
28
Client resistance
 Changes in the physical or psychological status of a
client can affect his/her resistance to infection.
 Any break in the integrity of the skin or mucous
membrane increase the chance of infection. Stress,
fatigue, poor nutrition and chronic illness also can
decrease the client ability to ward off infection.
 Adequate hygiene is important to decrease
microorganisms on the skin that could contribute to
infection risk.
4/9/2024
29
Key Points
 Nosocomial infections means infections acquired in health
care delivery settings
 Risk factors that contribute to the development of infections
must be considered when making attempts to decrease the
clients risk
 The incidence of infections associated with health care
delivery (nosocomial infections) can be decreased with good
infection practices
4/9/2024
30
Session Evaluation
Any questions?????
4/9/2024
31
References
 Greenwood, D., Slack, R. C. B., & Peutherer, J. F. (2002). Medical microbiology: A guide to
microbial infections: Pathogenesis, immunity, laboratory diagnosis and control (6th ed.).
Edinburgh: Churchill Livingstone.
 MOHSW. (2004). National infection prevention and control guidelines for health care services
in Tanzania. Dar es Salaam: Ministry of Health and Social Welfare.
 MOHSW. (2018). National infection prevention and control guidelines for health care services
in Tanzania: A pocket guide for health care providers. Dar es Salaam: Ministry of Health and
Social Welfare.
 Nester, E. W., Anderson, D. G., Roberts Jr., C. E., Pearsall, N. N., Nester, M. T., & Hurley, D.
(2004). Microbiology: A human perspective (4th ed.). Boston:
 McGraw Hill. Rosdahl, C. B. (1999). Textbook of basic nursing (7th ed.). Philadelphia:
Lippincott.
 Tietjen, L., Bossemeyer, D., & McIntosh, N. (2003). Infection prevention guidelines for
healthcare facilities with limited resources. Maryland: JHPIEGO Corporation.
4/9/2024
32

SESSION _1_INFECTION AND PREVENTION.pptx

  • 1.
    : Clinical Laboratory semesterII CMT 04211 BY JACOB NOAH LAB SCIENTIST, BSC IN HEALTH LAB SCIENCE, ACADEMIC TUTOR II ,RESEARCH OFFICER TAIFA INSTITUTE OF HEALTH AND ALLIED SCIENCES 4/9/2024 1
  • 2.
    LEARNING OBJECTIVES  Atthe end of this session each student is expected to be able to;-  Define IPC, health care acquired infections and Standard precaution  Outline objectives of IPC  Outline four major types of health care acquired infections  Explain risk factors for health care acquired infections  Explain components of standard precautions in IPC (consider every person, hand hygiene, personal protective equipment, handle sharps, safe dispose of wastes and process instruments) 4/9/2024 2
  • 3.
    INTRODUCTION  In healthcaresettings, there is a need for a practical, evidence-based approach for preventing patients and health workers from being harmed by avoidable infections  IPC is the aspect of health care which aims to ensure that patients do not contract infections as a result of attending health care facility for assessment, examination or treatment. 4/9/2024 3
  • 4.
    DEFINITIONS  HAIs, alsotermed nosocomial infections, are infections occurring in a patient during the process of care in a hospital or other health-care facility, which was not present or incubating at the time of admission. HCWs are also at risk for developing HAIs.  IPC programs are implemented to prevent HAIs from occurring and spreading within health facilities. 4/9/2024 4
  • 5.
     Standard precaution,are a simple set of effective practice guidelines (creating a physical, mechanical or chemical barrier) to protect health service provider and clients/patients from infection with a range of pathogens including blood borne. 4/9/2024 5
  • 6.
     Infection isthe successful entrance into the body by pathogenic microorganisms and the reaction of the body to the presence of these microorganisms.  OR Infection is the entry and development of many parasites or multiplication of an infectious agent in the body of humans or other animals.  Prevention is behaviors designed to avoid disease 4/9/2024 6
  • 7.
     Standard precaution,are a simple set of effective practice guidelines (creating a physical, mechanical or chemical barrier) to protect health service provider and clients/patients from infection with a range of of pathogens including blood borne 4/9/2024 7
  • 8.
    Objectives of InfectionPrevention and Control (IPC) in Health care Facility  To protect patients/ clients from nosocomial infections  To protect health care workers from occupational infections  To protect communities from infectious diseases  To prevent the environment from pollution 4/9/2024 8
  • 9.
    Components of StandardPrecautions in IPC  Consider every person (patient or health workers) as potentially infectious and susceptible to infection  Consider dead bodies as also potentially infectious  All items from all clients must be handled as if they are contaminated and all clients treated as if they may be infected  Use appropriate hand hygiene e.g. hand washing, Hand hygiene- this includes hand washing and hand antisepsis, antiseptic and hand scrub.  Wear personal protective equipment (PPE) including gloves, masks, goggles, caps, gowns, boots and aprons. 4/9/2024 9
  • 10.
     Handle sharps,patient resuscitation and patient care equipment, linen appropriately.  Manage patient placement and patient environmental cleaning appropriately  Safe disposal of infectious waste materials to protect those who handle them and prevent injury or spread to the community.  Process instruments by decontamination, cleaning and then either sterilize or HLD using recommended procedures 4/9/2024 10
  • 11.
    CLASSIFICATIONS OF HAIs Endogenous infection. Many microorganisms that cause HAIs come from the patient’s own body (the term normal flora/endogenous flora can be used to describe this).  Eg; bacteria normally present in the colon can gain entry to the urinary tract and cause urinary tract infections. 4/9/2024 11
  • 12.
     Endogenous infection.Many microorganisms that cause HAIs come from the patient’s own body (the term normal flora/endogenous flora can be used to describe this).  Eg; bacteria normally present in the colon can gain entry to the urinary tract and cause urinary tract infections. 4/9/2024 12
  • 13.
    CLASSIFICATIONS OF HAIsCont…  Exogenous infection. Result from the transfer of microorganisms to the patient or HCW from an external reservoir.  Eg; microorganisms can be transferred through direct contact with contaminated hands of HCWs and other patients (cross- contamination), contaminated instruments and needles, or the environment (exogenous flora). 4/9/2024 13
  • 14.
     Practicing handhygiene at the right moment and other elements of standard precautions can greatly reduce the frequency of cross contamination between patients and HCWs and thus reduce the incidence of infection. As with endogenous infection, measures to protect a persons natural resistance to infection can also help to reduce the likelihood of infection if cross transmission does occur. 4/9/2024 14
  • 15.
    COMMON HAIs  UrinaryTract Infection (UTI)  Urinary tract infection is one of the most common HAIs. Preventing UTI is a major factor in decreasing the overall incidence of HAIs in HCFs. Healthcare-associated UTIs are frequently related to urinary catheterization. Many patients with a urinary catheter develop bacteriuria (bacteria in the urine) because the catheter creates a pathway for bacteria to enter the bladder. 4/9/2024 15
  • 16.
     However, itis important to make the distinction between bacteriuria and an actual urinary tract infection. Patients should not be considered to have a catheter related urinary tract infection and should not receive antimicrobial treatment solely because the urine is discoloured, has an odour, or because the laboratory has cultured bacteria from the urine. Unless the patient has clinical features of infection (e.g. fever, rigors, other systemic features) they should not be considered to have catheter related UTI. 4/9/2024 16
  • 17.
    Factors that leadto UTI  Factors that can lead to bacteriuria and may lead to UTIs include:-  Urinary catheterization which creates a pathway that allows for endogenous transfer of microorganisms (e.g. bacteria from the patient’s GI tract can be transmitted to the urinary tract)  Passage of organisms from the urine bag to the bladder (retrograde contamination) can occur in patients with indwelling catheters  Some microorganisms that can grow on the outside or inside of the catheter’s tubing and in the urine itself  Handling of the urinary catheter and urine bag by HCWs 4/9/2024 17
  • 18.
    Reducing Hospital-Acquired UrinaryTract Infections:  Introducing an indwelling urinary catheter should be done only when necessary and no other options are effective. It is particularly important to limit the duration of catheterization as much as possible  Following appropriate procedures for inserting and removing urinary catheters will also reduce the risk of UTI 4/9/2024 18
  • 19.
    B: Infections (SSI Surgical site infections is often the result of contamination during the surgical procedure or contamination of the surgical wound after the procedure. SSIs are very common HAIs and often require additional surgical procedures to treat the infection. 4/9/2024 19
  • 20.
    The following factorspredispose a patient to development of a SSI:  Obesity  Infection at another body site at the time of surgery  Immunosuppression  Malnutrition and anaemia  Old age and chronic diseases such as diabetes and malignancy 4/9/2024 20
  • 21.
    Reducing SSIs inpatients:  Avoid prolonged preoperative hospitalization and recommend ambulatory surgery as often as possible  Avoid preoperative hair removal. If hair must be removed, clip it with scissors or electric clippers just before the surgery. Do not shave using a razor blade (shaving has been attributed to microscopic cuts in the skin that later serve as foci for bacterial multiplication)  In the surgical room prepare a wide area around the proposed incision site with antiseptic solution (2% alcohol chlorhexidine is generally appropriate)  Practice good surgical techniques that minimize tissue trauma, control bleeding, eliminate dead space, use minimal sutures, and maintain adequate blood supply and oxygenation  Keep the duration of surgical procedures as short as possible. The rate of infection doubles with each hour of surgery 4/9/2024 21
  • 22.
    C. Health Care-AssociatedPneumonia  Healthcare associated pneumonia (HAP) is a common HAI with a significant risk of a fatal outcome. Most of these infections occur by aspiration of bacteria growing in the back of the throat or in the stomach. Pneumonia associated with mechanical ventilation may be referred to as ventilator associated pneumonia (VAP). 4/9/2024 22
  • 23.
     The rangeof microorganisms associated with HAP/VAP is much wider than is the case for community acquired pneumonia (CAP) and many of these microorganisms are much more likely to be resistant to antimicrobials. Therefore HAP/VAP may be much harder to treat effectively with antimicrobial agents than CAP. 4/9/2024 23
  • 24.
    General activities employedin prevention and control of diseases  Standard precautions include:  Hand Hygiene  Personal Protective Equipment (PPE) appropriate for the level of care being given or the potential infection risk associated with an activity, even when there is no known risk of infection  Respiratory hygiene and cough etiquette  Injection and phlebotomy safety and sharps injury prevention 4/9/2024 24
  • 25.
    General activities employedin prevention and control of diseases  Safe decontamination and sterilization of medical equipment  safe handling of Linen and laundry  Environmental decontamination  Healthcare waste management 4/9/2024 25
  • 26.
    Risk Factors inNosocomial Infection  The longer the client is in a health care facility, the greater is her/his risk of infection.  Exposure to the facility environment changes the client own normal body flora  Risk factors that contribute to the development of nosocomial infections can be grouped into three categories: environment, therapeutic regimen, and resistance of the client 4/9/2024 26
  • 27.
    Environment  Hospital, outpatientclinics, extended care facilities, the home, and schools are reservoir of organisms that pose threat to the increasing number of clients who have decreased resistance.  The source of these organisms includes the air, other clients, families, and visitors, contaminated equipment, food and personnel.  Pneumonia and influenza can spread rapidly among clients and other people in all types of facilities.  Equipment that is not thoroughly cleaned, disinfected or sterilized can spread many pathogens. 4/9/2024 27
  • 28.
    Therapeutic regimen  Multiplefactors involved in therapies used to cure clients also can contribute to the risk of infection.  Drugs such as steroids, immunosuppressive agents, and cancer therapy, as well as prolonged use of antibiotics predispose clients to infection.  Equipment such as IV catheter, urinary catheters and feeding tubes that invade orifices provides routes for bacterial invasion  Inadequate dressing techniques for wound can provide media for bacterial growth.  Identifying treatments that pose risk and discontinuing their use as soon as possible decrease the chance of nosocomial infection 4/9/2024 28
  • 29.
    Client resistance  Changesin the physical or psychological status of a client can affect his/her resistance to infection.  Any break in the integrity of the skin or mucous membrane increase the chance of infection. Stress, fatigue, poor nutrition and chronic illness also can decrease the client ability to ward off infection.  Adequate hygiene is important to decrease microorganisms on the skin that could contribute to infection risk. 4/9/2024 29
  • 30.
    Key Points  Nosocomialinfections means infections acquired in health care delivery settings  Risk factors that contribute to the development of infections must be considered when making attempts to decrease the clients risk  The incidence of infections associated with health care delivery (nosocomial infections) can be decreased with good infection practices 4/9/2024 30
  • 31.
  • 32.
    References  Greenwood, D.,Slack, R. C. B., & Peutherer, J. F. (2002). Medical microbiology: A guide to microbial infections: Pathogenesis, immunity, laboratory diagnosis and control (6th ed.). Edinburgh: Churchill Livingstone.  MOHSW. (2004). National infection prevention and control guidelines for health care services in Tanzania. Dar es Salaam: Ministry of Health and Social Welfare.  MOHSW. (2018). National infection prevention and control guidelines for health care services in Tanzania: A pocket guide for health care providers. Dar es Salaam: Ministry of Health and Social Welfare.  Nester, E. W., Anderson, D. G., Roberts Jr., C. E., Pearsall, N. N., Nester, M. T., & Hurley, D. (2004). Microbiology: A human perspective (4th ed.). Boston:  McGraw Hill. Rosdahl, C. B. (1999). Textbook of basic nursing (7th ed.). Philadelphia: Lippincott.  Tietjen, L., Bossemeyer, D., & McIntosh, N. (2003). Infection prevention guidelines for healthcare facilities with limited resources. Maryland: JHPIEGO Corporation. 4/9/2024 32