This document discusses evaluating TMEM33 as a prognostic biomarker for cervical cancer and its correlation with immune infiltration. The author used four cervical cancer cell lines and one normal epithelial cell line, transfecting them with siRNAs. Western blot analysis and quantitative RT-PCR were used to detect changes in TMEM33 expression levels and proliferation rates with decreased TMEM33. The results suggest TMEM33 may be involved in cervical cancer progression and metastasis, and that the tumor microenvironment can stimulate tumor development by causing immune suppression and escape. The author concludes that evaluating diseases with multiple techniques can help determine appropriate treatments and develop more understanding of cancer behavior, and that TMEM33 shows potential as an early biomarker for cervical cancer.

1. Valentina Zapata Díaz
Third Semester
Molecular Biology
UPB

2. Cervical cancer
Introduction
cervical cancer is a disease that’s the third
principal cause of death in females.
It stars when the cervix cells go through
changes known as dysplasia, if those
abnormal cells aren´t destroyed they may
became cancer cells.
Cervical Cancer can progress from an early
stage to a more advanced stage if is not
treated quickly

3. Immune Infiltration
Immune infiltration is linked to
disease progression and chronicity.
Also immune cells infiltration
participate in the resolution of
inflammation, imposing a highly
dynamic view of the inherent process.

4. General Objective
Evaluate TMEM33 as a prognostic biomarker of cervical cancer and
its correlation with immnune infiltration

5. Methods
Cell lines and cell cultures Cell Transfection
To study cervical cancer there
were four different cell lines used
for cervical cancer and one cell
line for human normal epithelial
cell
HaCat
HeLa
SiHa
C-33A
Caski
SiRNAs were transferd into cells by using
lipofectamine
CC cell lines
Human normal
epithelial cell line

6. Methods
Western Blot Analysis
Quantitative RT- PCR
It’s s a technique used to detect
a specific protein using an
electrophoresis
It’s a process that can detect and
measure some patogens during
each cycle of the PCR process

7. Results
Expression of TMEM 33 in cervical
cancer cell lines
Changes of TMEM33
expressing levels

8. Results
Cell proliferation decreases
with TMEM 33
Immunofluorescence for cervical
cancer cell lines

9. Discussion
H.Chen, X. Zhao, Y.Li
TMEM33 may be involved in
the progression of
metastasis
K.G.K Deepak, R. Vempati,
G.P Nagaraju
X. Tian, H. Shen, Z. Li, T
Wang, S. Wang
Microenviroment can
stimulate the developments
of various tumors
Immunosupressive cells can
cause an immune escape in
TME that can promote
metastasis
AUTHORS STATEMENT STATUS

10. Conclusions
Evaluate a disease with different molecular biology techniques
could help to find the appropiate treatment acording to the
patient also can help to develope more information about cervical
cancer behavior
TMEM33 is an excellent biomarker that could predict the early
spawn of cervical cancer