miRNA Breast Cancer Prognosis -- Ingenuity SystemsNatalie Ng
1) The document describes research to develop microRNA prognostic signatures that can predict breast cancer metastasis and determine which patients would benefit from chemotherapy.
2) Through computational modeling of miRNA and mRNA expression data, the researcher identified 5-miRNA signatures for ER-positive and ER-negative breast cancer.
3) In vitro experiments on breast cancer cell lines validated that the expressions of miRNAs in the signatures correlated with metastatic characteristics like migration, invasion and proliferation as predicted by the computational models.
The study of genetic alterations of the signal transducing molecules and their role in the development and progression of Colorectal Cancer in Kashmir Valley
This document discusses how genomics is changing cancer care. It explains that while most cancers are sporadic, genomic testing can be used to identify hereditary cancer risk factors based on family history, pathology, and personal history. It also outlines how genomic analysis of tumor cells can provide information for prognosis, targeted therapy, and screening. The future of cancer care will likely involve more widespread, cheaper, and faster genomic testing to enable personalized treatment and pharmacogenomic testing.
This document provides an overview of molecular genetics and cancer biology concepts. It begins with an introduction to basic molecular genetics topics like DNA, genes, chromosomes, and gene expression. It then discusses how normal cells can become cancerous through genetic mutations that disrupt processes like tumor suppressor genes, oncogenes, the cell cycle, and DNA repair. The document emphasizes how understanding molecular genetics advances has helped improve diagnosis and treatment of cancers like prostate and bladder cancer, but also highlights ongoing challenges. It provides context on cancer rates and the need for continued research.
This document summarizes key information about X-linked diseases and mitochondrial DNA diseases. It discusses the X chromosome and genes located on it. It provides details on three specific X-linked diseases: hemophilia A, Duchenne muscular dystrophy, and fragile X syndrome. It describes the inheritance patterns, genetic causes, clinical features, and DNA testing approaches for each. It also provides a brief overview of the structure and function of mitochondrial DNA and its role in oxidative phosphorylation.
This document discusses cancer genetics and genomics. It covers several topics: the molecular bases of cancer; technologies to detect cancer alterations across the genome; predictive cancer genomics; and next-generation sequencing technologies in cancer research. Some key points include: somatic mutations occur in non-germline tissues and are non-heritable, while germline mutations are present in eggs or sperm and are heritable; cancer has several hallmarks including sustaining proliferative signaling, evading growth suppressors, resisting cell death, and more; high-throughput technologies can identify genomic and epigenomic aberrations in cancer; and next-generation sequencing is revolutionizing cancer research by allowing sequencing of entire tumor genomes.
This document summarizes research on Cadm1, a gene that suppresses metastasis. Genetic analysis identified a locus on mouse chromosome 9 associated with metastasis. Cadm1 was validated as a metastasis-suppressing gene within this locus. Ectopic expression of Cadm1 reduced pulmonary metastasis in vivo, while knockdown increased metastasis. Cadm1 expression was associated with better survival in human breast cancer datasets. The mechanism of Cadm1's effect involves interaction with the cell-mediated immune system through proteins like CRTAM that activate CD8+ T cells and NK cells, reducing metastasis in an immune-dependent manner.
miRNA Breast Cancer Prognosis -- Ingenuity SystemsNatalie Ng
1) The document describes research to develop microRNA prognostic signatures that can predict breast cancer metastasis and determine which patients would benefit from chemotherapy.
2) Through computational modeling of miRNA and mRNA expression data, the researcher identified 5-miRNA signatures for ER-positive and ER-negative breast cancer.
3) In vitro experiments on breast cancer cell lines validated that the expressions of miRNAs in the signatures correlated with metastatic characteristics like migration, invasion and proliferation as predicted by the computational models.
The study of genetic alterations of the signal transducing molecules and their role in the development and progression of Colorectal Cancer in Kashmir Valley
This document discusses how genomics is changing cancer care. It explains that while most cancers are sporadic, genomic testing can be used to identify hereditary cancer risk factors based on family history, pathology, and personal history. It also outlines how genomic analysis of tumor cells can provide information for prognosis, targeted therapy, and screening. The future of cancer care will likely involve more widespread, cheaper, and faster genomic testing to enable personalized treatment and pharmacogenomic testing.
This document provides an overview of molecular genetics and cancer biology concepts. It begins with an introduction to basic molecular genetics topics like DNA, genes, chromosomes, and gene expression. It then discusses how normal cells can become cancerous through genetic mutations that disrupt processes like tumor suppressor genes, oncogenes, the cell cycle, and DNA repair. The document emphasizes how understanding molecular genetics advances has helped improve diagnosis and treatment of cancers like prostate and bladder cancer, but also highlights ongoing challenges. It provides context on cancer rates and the need for continued research.
This document summarizes key information about X-linked diseases and mitochondrial DNA diseases. It discusses the X chromosome and genes located on it. It provides details on three specific X-linked diseases: hemophilia A, Duchenne muscular dystrophy, and fragile X syndrome. It describes the inheritance patterns, genetic causes, clinical features, and DNA testing approaches for each. It also provides a brief overview of the structure and function of mitochondrial DNA and its role in oxidative phosphorylation.
This document discusses cancer genetics and genomics. It covers several topics: the molecular bases of cancer; technologies to detect cancer alterations across the genome; predictive cancer genomics; and next-generation sequencing technologies in cancer research. Some key points include: somatic mutations occur in non-germline tissues and are non-heritable, while germline mutations are present in eggs or sperm and are heritable; cancer has several hallmarks including sustaining proliferative signaling, evading growth suppressors, resisting cell death, and more; high-throughput technologies can identify genomic and epigenomic aberrations in cancer; and next-generation sequencing is revolutionizing cancer research by allowing sequencing of entire tumor genomes.
This document summarizes research on Cadm1, a gene that suppresses metastasis. Genetic analysis identified a locus on mouse chromosome 9 associated with metastasis. Cadm1 was validated as a metastasis-suppressing gene within this locus. Ectopic expression of Cadm1 reduced pulmonary metastasis in vivo, while knockdown increased metastasis. Cadm1 expression was associated with better survival in human breast cancer datasets. The mechanism of Cadm1's effect involves interaction with the cell-mediated immune system through proteins like CRTAM that activate CD8+ T cells and NK cells, reducing metastasis in an immune-dependent manner.
This document discusses cervical cancer, its causes, symptoms, diagnosis, and treatment. Some key points:
- Cervical cancer is the fourth most common cancer in women worldwide and is caused by human papillomavirus (HPV) infection in over 90% of cases.
- Other risk factors include multiple sexual partners, young age of first intercourse, smoking, and a weakened immune system.
- Symptoms can include abnormal bleeding or discharge. Diagnosis involves exams, Pap tests, HPV tests, biopsies and assessing the cancer stage.
- Cervical cancer is typically treated through surgery, radiation therapy, chemotherapy, or a combination depending on the cancer stage and grade. Vaccines can
This document summarizes a study exploring the effects of baicalein (BAI) on bladder cancer cells. The study found that BAI inhibits proliferation and promotes apoptosis in bladder cancer cells. It also inhibits bladder cancer cell migration by down-regulating microRNA (miR)-106 expression. Specifically, BAI affects bladder cancer cells by inhibiting the JNK and MEK/ERK pathways through reducing miR-106 levels. P21 was also identified as a target of miR-106. The study utilized techniques like transfection, PCR, western blot analysis, and cell migration assays to analyze these regulatory mechanisms and effects of BAI on bladder cancer cells.
This document provides an overview of oncogenes and tumor suppressor genes and their role in cancer initiation and progression. It discusses how mutations in proto-oncogenes can activate them into oncogenes, causing increased or altered protein production and stimulating cell proliferation. Tumor suppressor genes normally inhibit cell growth but mutations can repress them, deregulating the cell cycle. The interactions between oncogene activation and tumor suppressor gene inactivation are required for full malignant transformation. Understanding these genetic factors involved in carcinogenesis can provide insights into cancer prevention, diagnosis, and treatment.
Cancer biology senescence & imortalisationGaurav Kumar
This document discusses tumor suppressor mechanisms and senescence. It notes that 1% of neonatal cord blood contains malignant clones and 1/3 of adults possess an IgH-BCL2 translocation responsible for follicular leukemia. Senescence is a process of permanent growth arrest in response to telomere erosion, DNA damage, or oncogene activation. Chromosome 9p21 contains the INK4a/ARF/INK4b locus that encodes the p16, p15, and ARF proteins involved in senescence. Telomeres form protective T-loops at chromosomal ends to prevent DNA damage. NUTLINS and DNA methyltransferase inhibitors can reactivate p53 and p15/
This document discusses molecular profiling of breast cancer. It begins by introducing breast cancer as the most common cancer in women. It then discusses traditional classifications based on histological and clinical features. However, up to half of hormone receptor positive cancers do not respond to treatment, showing clinical classifications are insufficient. Molecular profiling uses high-throughput techniques to better understand breast cancer biology and refine classifications. Gene expression profiling has identified major molecular subtypes, like luminal A/B, HER2-positive, and basal-like. Multigene assays provide prognostic and predictive information beyond traditional clinics-pathological factors. Several common assays are discussed, including Oncotype DX, Mammaprint, and PAM50. Next generation sequencing is also discussed for
1. Immuno histochemistry is used to detect antigens of interest in tissue sections using antibodies and helps in subtyping cancers of unknown primary, determining prognosis, and guiding personalized treatment.
2. Key markers used in gynecologic cancers include cytokeratins and vimentin to identify epithelial and mesenchymal differentiation, ER and PR to subtype endometrial cancers, and P53 and P16 patterns to distinguish endometrial cancer subtypes.
3. Negative expression of markers like PTEN can also be informative in distinguishing lesions and predicting progression in cancers like endometrial cancer.
Carcinogenesis
Theories of carcinogenesis
Hallmarks of cancer
Important Oncogenes
RB & p53 genes
Metastasis
Aetiology and Pathogenesis of cancer
Tests for carcinogenicity
How to repair damaged DNA?
Basic DNA repair mechanism
Repair of double stranded break
This document discusses chemical carcinogenesis and the initiation and promotion stages. Initiation results from exposure to a carcinogenic agent and causes permanent DNA damage (mutations). Promotion alone cannot cause tumors, but can induce tumors in initiated cells by causing proliferation and clonal expansion of initiated cells. The document also discusses various carcinogens like radiation, viruses, and bacteria that can cause cancer through direct DNA damage or indirect mechanisms like chronic inflammation. It also summarizes tumor immunity, how tumors evade the immune system, and some clinical aspects of cancer like paraneoplastic syndromes and cachexia.
Microsatellite instability testing is an important part in diagnostics in Metastatic cancer settings after the FDA has given approval for tissue agnostic indications in almost all solid cancers. MSI by PCR and MMR status by IHC is also helpful for evaluation of genetic risk in Colon and Endometrial cancers
Identification of cancer drivers across tumor typesNuria Lopez-Bigas
Thousands of tumor genomes/exomes are being sequenced as part of the International Cancer Genome Consortium (ICGC), The Cancer Genome Atlas (TCGA) and other initiatives. This opens the possibility to have, for the first time, a comprehensive picture of mutations, genes and pathways involved in the cancer phenotype across tumor types. We have developed computational methods able to identify signals of positive selection in the pattern of tumor somatic mutations, which point to genes and pathways directly involved in the development of the tumors. We have applied these approaches to 3025 tumors from 12 different cancer types of the TCGA Pan-Cancer project, identifying 291 high-confidence cancer driver genes acting on those tumors (Tamborero et al 2013). We have also developed IntOGen-mutations (http://www.intogen.org/mutations), a novel web platform for cancer genomes interpretations, which analyses not only TCGA pan-cancer data but all mutation data from ICGC and other initiatives. The resource allows users to identify driver mutations, genes and pathways acting on more than 6000 tumors originated in 17 different cancer sites and to analyze newly sequence tumor genomes. Among the novel cancer drivers identified there are chromatin regulatory factors and splicing factors, which are emerging as important genes in cancer development and are regarded as interesting candidates for novel targets for cancer treatment. In my talk I will summarize all these recent findings.
More info: http://bg.upf.edu/blog/2013/10/my-slides-on-identification-of-cancer-drivers-across-tumor-types/
Differences in microRNA expression during tumor development in the transition...Enrique Moreno Gonzalez
The prostate is divided into three glandular zones, the peripheral zone (PZ), the transition zone (TZ), and the central zone. Most prostate tumors arise in the peripheral zone (70-75%) and in the transition zone (20-25%) while only 10% arise in the central zone. The aim of this study was to investigate if differences in miRNA expression could be a possible explanation for the difference in propensity of tumors in the zones of the prostate.
Breast cancer is caused by heterogeneous tumor cells whose behavior depends on biological features. Molecular subtyping through gene expression profiling can classify tumor types, recognize hereditary implications, identify appropriate therapies, determine prognosis, and avoid unnecessary treatment. The major subtypes are luminal A/B, HER2-enriched, and basal-like, which differ in gene expression, sensitivity to therapies, and clinical outcomes. Understanding the molecular biology of breast cancer is crucial for precision medicine approaches to management.
Acute myeloid leukemia (AML) is a hematopoietic malignancy with a dismal outcome in the majority of cases. A detailed understanding of the genetic alterations and gene expression changes that contribute to its pathogenesis is important to improve prognostication, disease monitoring, and therapy. In this context, leukemia-associated misexpression of microRNAs (miRNAs) has been studied, but no coherent picture has emerged yet, thus warranting further investigations.
The document discusses the molecular pathology of breast carcinoma. It describes the cellular types in the mammary gland, identification of mammary stem cells, and the epithelial cell hierarchy model. It also covers molecular classification of breast cancer, luminal and basal-like phenotypes, mechanisms of E-cadherin inactivation in lobular carcinoma, breast cancer in hereditary diffuse gastric cancer patients, and morphological and immunohistochemical features of BRCA1 and BRCA2 breast carcinomas.
A germline mutation in the brca1 3'utr predicts stage iv breast cancerDavid W. Salzman
1) A variant in the 3' untranslated region (3'UTR) of the BRCA1 gene was found to predict increased risk of stage IV breast cancer.
2) In vitro luciferase assays showed the variant reduced BRCA1 expression and altered response to stimuli compared to the normal 3'UTR.
3) Analysis of breast tumor tissue found reduced BRCA1 expression in patients with the variant, and patients with the variant had a 4-fold increased risk of stage IV disease.
Mohammad Rahman is a senior research associate at Ohio State University studying cancer metastasis. He has a PhD in cancer biology from Shimane Medical University in Japan and has held several postdoctoral research positions. His research focuses on how exosomes secreted by cancer cells drive metastasis by inducing epithelial to mesenchymal transition in other cells. He recently discovered that lung cancer cell exosomes can induce EMT in recipient cells, which may be important for establishing the tumor microenvironment and cancer spread. The goal of his current research proposal is to investigate the mechanisms by which cancer-derived exosomal contents drive metastasis.
Mohammad Rahman is a senior research associate at Ohio State University studying cancer metastasis. He has a PhD in cancer biology from Shimane Medical University in Japan and has held several postdoctoral research positions. His research focuses on how exosomes secreted by cancer cells drive metastasis by inducing epithelial to mesenchymal transition in other cells. He recently discovered that lung cancer cell exosomes can induce EMT in recipient cells, which may be important for establishing the tumor microenvironment and cancer spread. The goal of his current research proposal is to investigate the mechanisms by which cancer-derived exosomal contents drive metastasis.
This document discusses gene therapy as a promising treatment for breast cancer. It begins by providing background on breast cancer prevalence and current treatment options. It then describes how gene therapy could target mutated genes, cancer cell markers, block metastasis, and induce apoptosis. The document focuses on gene therapy approaches for breast cancer, including using antisense technology to inhibit pathogenic genes, RNA interference to downregulate genes like c-myc, and suicide genes to make cancer cells more sensitive to chemotherapy.
This document provides an overview of ovarian cancer, including risk factors, pathology, diagnosis, screening, staging, and management. Some key points include:
- Ovarian cancer accounts for 3-4% of cancers in women and is the fourth leading cause of cancer death.
- Risk factors include family history, ethnicity, reproductive history, and use of hormones.
- Diagnosis involves physical exam, tumor markers like CA-125, ultrasound, CT or MRI to determine if a mass is benign or malignant.
- Staging follows the FIGO system from I to IV depending on extent of spread. Surgery and chemotherapy are the primary treatments.
This document discusses cervical cancer, its causes, symptoms, diagnosis, and treatment. Some key points:
- Cervical cancer is the fourth most common cancer in women worldwide and is caused by human papillomavirus (HPV) infection in over 90% of cases.
- Other risk factors include multiple sexual partners, young age of first intercourse, smoking, and a weakened immune system.
- Symptoms can include abnormal bleeding or discharge. Diagnosis involves exams, Pap tests, HPV tests, biopsies and assessing the cancer stage.
- Cervical cancer is typically treated through surgery, radiation therapy, chemotherapy, or a combination depending on the cancer stage and grade. Vaccines can
This document summarizes a study exploring the effects of baicalein (BAI) on bladder cancer cells. The study found that BAI inhibits proliferation and promotes apoptosis in bladder cancer cells. It also inhibits bladder cancer cell migration by down-regulating microRNA (miR)-106 expression. Specifically, BAI affects bladder cancer cells by inhibiting the JNK and MEK/ERK pathways through reducing miR-106 levels. P21 was also identified as a target of miR-106. The study utilized techniques like transfection, PCR, western blot analysis, and cell migration assays to analyze these regulatory mechanisms and effects of BAI on bladder cancer cells.
This document provides an overview of oncogenes and tumor suppressor genes and their role in cancer initiation and progression. It discusses how mutations in proto-oncogenes can activate them into oncogenes, causing increased or altered protein production and stimulating cell proliferation. Tumor suppressor genes normally inhibit cell growth but mutations can repress them, deregulating the cell cycle. The interactions between oncogene activation and tumor suppressor gene inactivation are required for full malignant transformation. Understanding these genetic factors involved in carcinogenesis can provide insights into cancer prevention, diagnosis, and treatment.
Cancer biology senescence & imortalisationGaurav Kumar
This document discusses tumor suppressor mechanisms and senescence. It notes that 1% of neonatal cord blood contains malignant clones and 1/3 of adults possess an IgH-BCL2 translocation responsible for follicular leukemia. Senescence is a process of permanent growth arrest in response to telomere erosion, DNA damage, or oncogene activation. Chromosome 9p21 contains the INK4a/ARF/INK4b locus that encodes the p16, p15, and ARF proteins involved in senescence. Telomeres form protective T-loops at chromosomal ends to prevent DNA damage. NUTLINS and DNA methyltransferase inhibitors can reactivate p53 and p15/
This document discusses molecular profiling of breast cancer. It begins by introducing breast cancer as the most common cancer in women. It then discusses traditional classifications based on histological and clinical features. However, up to half of hormone receptor positive cancers do not respond to treatment, showing clinical classifications are insufficient. Molecular profiling uses high-throughput techniques to better understand breast cancer biology and refine classifications. Gene expression profiling has identified major molecular subtypes, like luminal A/B, HER2-positive, and basal-like. Multigene assays provide prognostic and predictive information beyond traditional clinics-pathological factors. Several common assays are discussed, including Oncotype DX, Mammaprint, and PAM50. Next generation sequencing is also discussed for
1. Immuno histochemistry is used to detect antigens of interest in tissue sections using antibodies and helps in subtyping cancers of unknown primary, determining prognosis, and guiding personalized treatment.
2. Key markers used in gynecologic cancers include cytokeratins and vimentin to identify epithelial and mesenchymal differentiation, ER and PR to subtype endometrial cancers, and P53 and P16 patterns to distinguish endometrial cancer subtypes.
3. Negative expression of markers like PTEN can also be informative in distinguishing lesions and predicting progression in cancers like endometrial cancer.
Carcinogenesis
Theories of carcinogenesis
Hallmarks of cancer
Important Oncogenes
RB & p53 genes
Metastasis
Aetiology and Pathogenesis of cancer
Tests for carcinogenicity
How to repair damaged DNA?
Basic DNA repair mechanism
Repair of double stranded break
This document discusses chemical carcinogenesis and the initiation and promotion stages. Initiation results from exposure to a carcinogenic agent and causes permanent DNA damage (mutations). Promotion alone cannot cause tumors, but can induce tumors in initiated cells by causing proliferation and clonal expansion of initiated cells. The document also discusses various carcinogens like radiation, viruses, and bacteria that can cause cancer through direct DNA damage or indirect mechanisms like chronic inflammation. It also summarizes tumor immunity, how tumors evade the immune system, and some clinical aspects of cancer like paraneoplastic syndromes and cachexia.
Microsatellite instability testing is an important part in diagnostics in Metastatic cancer settings after the FDA has given approval for tissue agnostic indications in almost all solid cancers. MSI by PCR and MMR status by IHC is also helpful for evaluation of genetic risk in Colon and Endometrial cancers
Identification of cancer drivers across tumor typesNuria Lopez-Bigas
Thousands of tumor genomes/exomes are being sequenced as part of the International Cancer Genome Consortium (ICGC), The Cancer Genome Atlas (TCGA) and other initiatives. This opens the possibility to have, for the first time, a comprehensive picture of mutations, genes and pathways involved in the cancer phenotype across tumor types. We have developed computational methods able to identify signals of positive selection in the pattern of tumor somatic mutations, which point to genes and pathways directly involved in the development of the tumors. We have applied these approaches to 3025 tumors from 12 different cancer types of the TCGA Pan-Cancer project, identifying 291 high-confidence cancer driver genes acting on those tumors (Tamborero et al 2013). We have also developed IntOGen-mutations (http://www.intogen.org/mutations), a novel web platform for cancer genomes interpretations, which analyses not only TCGA pan-cancer data but all mutation data from ICGC and other initiatives. The resource allows users to identify driver mutations, genes and pathways acting on more than 6000 tumors originated in 17 different cancer sites and to analyze newly sequence tumor genomes. Among the novel cancer drivers identified there are chromatin regulatory factors and splicing factors, which are emerging as important genes in cancer development and are regarded as interesting candidates for novel targets for cancer treatment. In my talk I will summarize all these recent findings.
More info: http://bg.upf.edu/blog/2013/10/my-slides-on-identification-of-cancer-drivers-across-tumor-types/
Differences in microRNA expression during tumor development in the transition...Enrique Moreno Gonzalez
The prostate is divided into three glandular zones, the peripheral zone (PZ), the transition zone (TZ), and the central zone. Most prostate tumors arise in the peripheral zone (70-75%) and in the transition zone (20-25%) while only 10% arise in the central zone. The aim of this study was to investigate if differences in miRNA expression could be a possible explanation for the difference in propensity of tumors in the zones of the prostate.
Breast cancer is caused by heterogeneous tumor cells whose behavior depends on biological features. Molecular subtyping through gene expression profiling can classify tumor types, recognize hereditary implications, identify appropriate therapies, determine prognosis, and avoid unnecessary treatment. The major subtypes are luminal A/B, HER2-enriched, and basal-like, which differ in gene expression, sensitivity to therapies, and clinical outcomes. Understanding the molecular biology of breast cancer is crucial for precision medicine approaches to management.
Acute myeloid leukemia (AML) is a hematopoietic malignancy with a dismal outcome in the majority of cases. A detailed understanding of the genetic alterations and gene expression changes that contribute to its pathogenesis is important to improve prognostication, disease monitoring, and therapy. In this context, leukemia-associated misexpression of microRNAs (miRNAs) has been studied, but no coherent picture has emerged yet, thus warranting further investigations.
The document discusses the molecular pathology of breast carcinoma. It describes the cellular types in the mammary gland, identification of mammary stem cells, and the epithelial cell hierarchy model. It also covers molecular classification of breast cancer, luminal and basal-like phenotypes, mechanisms of E-cadherin inactivation in lobular carcinoma, breast cancer in hereditary diffuse gastric cancer patients, and morphological and immunohistochemical features of BRCA1 and BRCA2 breast carcinomas.
A germline mutation in the brca1 3'utr predicts stage iv breast cancerDavid W. Salzman
1) A variant in the 3' untranslated region (3'UTR) of the BRCA1 gene was found to predict increased risk of stage IV breast cancer.
2) In vitro luciferase assays showed the variant reduced BRCA1 expression and altered response to stimuli compared to the normal 3'UTR.
3) Analysis of breast tumor tissue found reduced BRCA1 expression in patients with the variant, and patients with the variant had a 4-fold increased risk of stage IV disease.
Mohammad Rahman is a senior research associate at Ohio State University studying cancer metastasis. He has a PhD in cancer biology from Shimane Medical University in Japan and has held several postdoctoral research positions. His research focuses on how exosomes secreted by cancer cells drive metastasis by inducing epithelial to mesenchymal transition in other cells. He recently discovered that lung cancer cell exosomes can induce EMT in recipient cells, which may be important for establishing the tumor microenvironment and cancer spread. The goal of his current research proposal is to investigate the mechanisms by which cancer-derived exosomal contents drive metastasis.
Mohammad Rahman is a senior research associate at Ohio State University studying cancer metastasis. He has a PhD in cancer biology from Shimane Medical University in Japan and has held several postdoctoral research positions. His research focuses on how exosomes secreted by cancer cells drive metastasis by inducing epithelial to mesenchymal transition in other cells. He recently discovered that lung cancer cell exosomes can induce EMT in recipient cells, which may be important for establishing the tumor microenvironment and cancer spread. The goal of his current research proposal is to investigate the mechanisms by which cancer-derived exosomal contents drive metastasis.
This document discusses gene therapy as a promising treatment for breast cancer. It begins by providing background on breast cancer prevalence and current treatment options. It then describes how gene therapy could target mutated genes, cancer cell markers, block metastasis, and induce apoptosis. The document focuses on gene therapy approaches for breast cancer, including using antisense technology to inhibit pathogenic genes, RNA interference to downregulate genes like c-myc, and suicide genes to make cancer cells more sensitive to chemotherapy.
This document provides an overview of ovarian cancer, including risk factors, pathology, diagnosis, screening, staging, and management. Some key points include:
- Ovarian cancer accounts for 3-4% of cancers in women and is the fourth leading cause of cancer death.
- Risk factors include family history, ethnicity, reproductive history, and use of hormones.
- Diagnosis involves physical exam, tumor markers like CA-125, ultrasound, CT or MRI to determine if a mass is benign or malignant.
- Staging follows the FIGO system from I to IV depending on extent of spread. Surgery and chemotherapy are the primary treatments.
Ovarian cancer accounts for 3-4% of cancers in women and is the fourth most common cause of cancer death in females in the US. Some key points about ovarian cancer include that it typically presents at an advanced stage, the strongest risk factor is family history, and the main types are epithelial tumors, germ cell tumors, and stromal tumors. Diagnosis involves tumor markers like CA-125, ultrasound, and CT or MRI imaging to determine the stage of disease.
a nice presentation about the Ovarian Cancer its include an introduction with brief notes about the epidemiology and risk factors then shift to pathology and pathogenesis and diagnosis with signs , symptoms and lab tests with imaging modules , screening , management
MANAGEMENT OF EARLY STAGE OVARIAN MALIGNANCY.pptAdeniyiAkiseku
This document discusses the management of early stage ovarian malignancy. It covers the epidemiology, risk factors, clinical presentation, staging, and treatment options for early stage ovarian cancer. Standard treatment involves total abdominal hysterectomy, bilateral salpingo-oophorectomy, and infracolic omentectomy. For young women who wish to preserve fertility, fertility-sparing surgery may be an option for stage 1A disease less than 35 years old with certain criteria met. Chemotherapy is also sometimes recommended post-surgery to improve survival outcomes.
asmi gyn.pptx about ovarian cancer gynaecologyAsmitajha12
Ovarian cancer accounts for 3-4% of cancers in women and is the fourth most common cause of cancer death. Family history and genetic factors significantly increase risk. Symptoms are often vague until late stages when the cancer has spread. Diagnosis involves imaging tests and cancer antigen (CA125) blood levels. Most cancers are diagnosed at late stages. Treatment involves surgery to remove the ovaries and other organs, followed by chemotherapy. Despite aggressive treatment, survival rates remain low due to late stage diagnosis. Screening high-risk women aims to detect cancers earlier when treatment is most effective.
Ovarian cancer accounts for 3-4% of cancers in women and is the fourth most common cause of cancer death in women in the US. There are several risk factors for ovarian cancer including family history, ethnicity, reproductive history, and use of hormones. Ovarian cancers are generally divided into epithelial, germ cell, and stromal cell tumors. Early symptoms are vague but may include pelvic pain or pressure, back pain, bloating, and digestive issues. As the cancer progresses, symptoms worsen and may include abdominal swelling, weight loss, and changes in bowel or urinary habits. Diagnosis involves physical exam, tumor marker tests, ultrasound or CT imaging, and surgical staging to determine if the cancer
- Ovarian cancer is the ninth most common cancer in women and the fifth leading cause of cancer death in women. Risk factors include age over 60, obesity, talcum powder use, fertility drugs, genetic predispositions like BRCA mutations.
- Surgical staging is essential for determining prognosis and appropriate treatment. For early stage disease adjuvant chemotherapy is recommended. Advanced stage disease is treated with cytoreductive surgery followed by platinum/taxane chemotherapy.
- Prognosis depends on stage and completeness of cytoreduction. Median survival is 39 months for optimal vs 17 months for suboptimal cytoreduction. Secondary surgery and chemotherapy may provide benefit for recurrence in some patients.
This document discusses ovarian cancer prevention. It notes that ovarian cancer risk increases with age and factors like family history and endometriosis. Screening is not recommended for average risk women as trials found it did not reduce mortality and caused harm. Two main types of ovarian cancer have different origins - type II tumors often originate in the fallopian tubes. Prevention strategies discussed include risk-reducing salpingo-oophorectomies, oral contraceptives, metformin, NSAIDs, vitamins, and physical activity, which may reduce inflammation and hormones linked to cancer.
Fertility Preservation In Cancer Pt Fin (2)guest7f0a3a
This document summarizes fertility preservation techniques for cancer patients, including embryo freezing, oocyte freezing, and ovarian tissue banking. It discusses methods such as slow freezing versus vitrification for oocyte cryopreservation. The document also reviews factors that influence chemotherapy-induced premature menopause and the potential protective effects of GnRH analogues. Surgical procedures for fertility preservation like ovarian transposition are also mentioned.
Fertility Preservation In Cancer Pt Fin (1)guest7f0a3a
The document discusses several methods for preserving fertility in cancer patients, including:
1) Embryo freezing and ovarian tissue banking which allow patients to preserve eggs or embryos before cancer treatment.
2) The use of gonadotropin-releasing hormone agonists during chemotherapy to protect ovarian follicles from damage in adolescent patients. Studies show this method led to continued normal menstrual cycles after treatment.
3) Cryopreservation of immature or mature eggs through slow cooling or vitrification techniques, though success rates for establishing pregnancies from frozen eggs remain low. Further research is still needed to improve egg freezing outcomes.
Fertility Preservation In Cancer Pt Finguest7f0a3a
The document discusses several methods for preserving fertility in cancer patients, including:
1) Embryo freezing and ovarian tissue banking which allow patients to preserve eggs or embryos before cancer treatment.
2) The use of gonadotropin-releasing hormone agonists (GnRHa) during chemotherapy to protect ovarian follicles, based on a preliminary study showing it effectively preserved fertility.
3) Cryopreservation of immature or mature eggs, though success rates for mature egg freezing are still low due to challenges with ice crystal formation during slow freezing methods. New vitrification techniques may improve survival rates.
Ovarian cancer is the fourth most common cause of cancer death in women. The majority (70%) of cases are diagnosed at an advanced stage. Epithelial cancer accounts for 90% of ovarian cancers and has four main histological subtypes: serous, mucinous, endometrioid, and clear cell. Screening is recommended for women at high risk for familial ovarian cancer, such as those with BRCA gene mutations. Staging involves surgical assessment and determining the extent of disease spread. Primary treatment is surgical staging and debulking followed by chemotherapy.
The document provides information about the International Gynecologic Cancer Society (IGCS):
- It was founded in 1986 and has over 1500 multidisciplinary members from over 80 countries.
- Its mission is to promote women's health related to gynecologic cancers through research, treatment standards, and education.
- It publishes the International Journal of Gynecological Cancer and holds biennial conferences rotating between regions of the world.
Breast cancer arises from the breast tissues, usually the ducts or lobules. Several risk factors are associated with breast cancer development, including genetic mutations, hormone exposure, and lifestyle factors. The disease is usually diagnosed through screening mammography followed by diagnostic tests and staging to determine prognosis and guide treatment. Treatment options depend on cancer stage and characteristics, and may involve surgery, radiation, chemotherapy, hormone therapy, or targeted therapy.
This document summarizes information about breast cancer, including:
1. It discusses the classification, epidemiology, symptoms, risk factors, anatomy, pathology, stages, diagnosis, treatment and screening of breast cancer.
2. Key points include that breast cancer is the uncontrolled growth of abnormal cells in the breast, and is the second leading cause of cancer deaths in women. Risk factors include age, family history, obesity, lifestyle factors and genetics.
3. Diagnosis involves physical exams, mammograms, biopsies and tests for biomarkers. Treatment includes surgery, chemotherapy, radiation therapy and hormone therapy. Early detection through screening and awareness of risk factors can improve outcomes.
This document summarizes information about breast cancer, including:
1. It discusses the classification, epidemiology, symptoms, risk factors, anatomy, pathology, stages, diagnosis, treatment and screening of breast cancer.
2. Key points include that breast cancer is the uncontrolled growth of abnormal cells in the breast, and is the second leading cause of cancer deaths in women. Risk factors include age, family history, obesity, lifestyle factors and genetics.
3. Diagnosis involves physical exams, mammograms, biopsies and tests for hormone receptors. Treatment includes surgery, chemotherapy, radiation and hormone therapy. Early detection through screening and awareness of risk factors can improve outcomes.
- Ovarian cancer is the 4th leading cause of cancer death in women in the US, with a 5-year survival rate of only 35% for advanced cases. Most cases are diagnosed at an advanced stage due to non-specific early symptoms.
- There is no consensus on screening guidelines due to a lack of evidence that screening reduces mortality. Current screening methods like ultrasound and CA-125 lack sensitivity and specificity.
- Several large trials are underway to evaluate new screening strategies using ultrasound, tumor markers, and genetic testing to enable earlier detection when treatment is most effective. Improved screening methods are needed to reduce ovarian cancer mortality rates.
Breast carcinoma overview and recent advances in managementMurthy Murthy(Jnv)
Breast cancer is the second most common cancer affecting females worldwide. Risk factors include age, family history, genetic factors, reproductive history, and lifestyle factors. The document discusses the incidence, risk factors, etiology, clinical features, diagnostic workup, management, and recent advances in breast cancer. Biomarkers, imaging modalities, surgical approaches, chemotherapy, and targeted therapies are evolving to improve diagnosis and treatment outcomes in breast cancer.
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
Histololgy of Female Reproductive System.pptxAyeshaZaid1
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
Cell Therapy Expansion and Challenges in Autoimmune DiseaseHealth Advances
There is increasing confidence that cell therapies will soon play a role in the treatment of autoimmune disorders, but the extent of this impact remains to be seen. Early readouts on autologous CAR-Ts in lupus are encouraging, but manufacturing and cost limitations are likely to restrict access to highly refractory patients. Allogeneic CAR-Ts have the potential to broaden access to earlier lines of treatment due to their inherent cost benefits, however they will need to demonstrate comparable or improved efficacy to established modalities.
In addition to infrastructure and capacity constraints, CAR-Ts face a very different risk-benefit dynamic in autoimmune compared to oncology, highlighting the need for tolerable therapies with low adverse event risk. CAR-NK and Treg-based therapies are also being developed in certain autoimmune disorders and may demonstrate favorable safety profiles. Several novel non-cell therapies such as bispecific antibodies, nanobodies, and RNAi drugs, may also offer future alternative competitive solutions with variable value propositions.
Widespread adoption of cell therapies will not only require strong efficacy and safety data, but also adapted pricing and access strategies. At oncology-based price points, CAR-Ts are unlikely to achieve broad market access in autoimmune disorders, with eligible patient populations that are potentially orders of magnitude greater than the number of currently addressable cancer patients. Developers have made strides towards reducing cell therapy COGS while improving manufacturing efficiency, but payors will inevitably restrict access until more sustainable pricing is achieved.
Despite these headwinds, industry leaders and investors remain confident that cell therapies are poised to address significant unmet need in patients suffering from autoimmune disorders. However, the extent of this impact on the treatment landscape remains to be seen, as the industry rapidly approaches an inflection point.
TEST BANK For Community Health Nursing A Canadian Perspective, 5th Edition by...Donc Test
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8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
2. Outline
Introduction
Risk factors
Molecular Origin
The Molecular Of Mechanism
and Molecular Pathways
Guidelines
3. Introduction
Mohsen Koolivand MSc
Ovarian
Cancer
3–4% of cancer in women
estimated 22,000 new cases
Second most common gynecologic
malignancy
a disease of the postmenopausal
Early stage (I/II) detection has a survival
rate of over 90%
Symptoms are complex and often
misdiagnosed as other diseases
cellular and molecular characteristics,
identifying appropriate
block lymphatic vessels
4. Types Of Ovarian Tumors
The ovaries contain 3 main kinds of cells:
1. Epithelial
2. Germ cells
3. Stromal cells
Subsequently there are 3 main types of ovarian tumours:
1. Epithelial tumours
2. Germ cell tumours
3. Stromal tumours
5. Pelvic or abdominal pain
Symptoms
BloatingUrgent need to urinate
Frequent urination
abnormal menstrual cyclesSense of pelvic heaviness Back pain
Vaginal bleeding
7. Family History
The strongest risk factor
A women with a single first-degree relative with ov.Ca has a relative
risk (RR) of approximately 3.6 for developing ov.ca compared with
general population
The gene can be inherited through either the maternal or paternal
line, but has variable penetrance
9. Ethnicity
Higher in white women
Higher in north America and northern Europe
BRCA1 and BRCA2 genes are more common among white women
of Ashkenazi descent
Incidence of ov.ca is higher in countries with higher in countries with
higher per capita consumption of animal fat
10. Reproduction factors
Nulliparous
First childbirth after age 35 years
Involuntary infertility
Late menopause and early menarche
Pts. With prolonged period or uninterrupted ovulation
11. Others
Exogenous hormones :- HRT
Dietary factors , Diets high in saturated animal fats seem to confer
an increased risk by unknown mechanisms …
# Japanese women who move to the United States have an
increased ovarian cancer risk.
<<<<<<<<<<<<<<<<
12. Protective Factors
Multiparity: First pregnancy before age 30
Oral contraceptives: 5 years of use cuts risk nearly in half
Tubal ligation
Hysterectomy
Bilateral oopherectomy
Lactation
Epidemiologic and laboratory evidence suggest a potential role for
retinoids , vitamin D, NSAIDs as preventive agents for ovarian
cancer
13. According to International Federation of Gynecology and Obstetrics
(FIGO) Staging of Ovarian Neoplasms :-
Stage I. Growth limited to the ovaries
Ia —one ovary involved
Ib —both ovaries involved
Ic —Ia or Ib and ovarian surface tumor, ruptured capsule,
malignant ascites, or peritoneal cytology positive for malignant cells
Staging …
15. Stage II. Extension of the neoplasm from the ovary to the
pelvis
IIa—extension to the uterus or fallopian tube
IIb—extension to other pelvic tissues
IIc—IIa or b and ovarian surface tumor, ruptured capsule, malignant
ascites, or peritoneal cytology positive for malignant cells
Staging …
17. Stage III. Disease extension to the abdominal cavity
IIIa—abdominal peritoneal surfaces with microscopic metastases
IIIb—tumor metastases < 2 cm in size
IIIc—tumor metastases > 2 cm in size or metastatic disease in the
pelvic, paraaortic, or inguinal lymph nodes
Staging …
19. Stage IV. Distant metastatic disease
Malignant pleural effusion
Pulmonary parenchymal metastases
Liver or splenic parenchymal metastases (not surface implants)
Metastases to the supraclavicular lymph nodes or skin
Staging …
20. molecular origins of cancer, such as ovarian cancer is influenced
by a complex signaling pathway.
Ovarian cancer is also heterogeneous — multiple genetic and
epigenetic changes are evident in patients with ovarian cancer;
however, how such changes are selected for during tumorigenesis
is not yet clear.
Molecular Origin
21.
22.
23.
24. Molecular Pathways
Ovarian cancer is also heterogeneous
— multiple genetic and epigenetic
changes are evident in patients with
ovarian cancer; however, how such
changes are selected for during
tumorigenesis is not yet clear.
Several genetic alterations cause of
high genetic instability in ovarian cancer:
26. Sparc
SPARC Is a Key Regulator of Proliferation, Apoptosis and In
vasion in Human Ovarian Cancer
Secreted protein acidic and rich in cysteine (SPARC), a calcium-
binding matricellular glycoprotein, is implicated
in the progression of many cancers. investigations show
expression and function of SPARC in ovarian cancer.
27. PTEN
PTEN activity can also be lost through other
mechanisms such as epigenetic changes or post-
translational modifications or mutation .
28. BRCA1 and BRCA2
BRCA1 and BRCA2 suggests that they are involved in two
fundamental cellular processes: DNA damage repair and
transcriptional regulation.
33. KRAS
RAS proteins are central mediators downstream of growth factor
receptor signaling and therefore are critical for cell proliferation,
survival, and differentiation.
KRAS mutations are found in approximately 40% of patients with Type
I EOC tumors. In the majority of cases, these mutations are missense
mutations which introduce an amino acid substitution at position 12,
13, or 61. The result of these mutations is constitutive activation of
KRAS signaling pathways.
34.
35. PI3K
Activation of the PI3K pathway regulates:
Cell growth
Cell proliferation
Cell survival
The PI3K/Akt/mTOR pathway is frequently deregulated in OC
The expression levels of both PIK3CA and phosphorylated Akt (pAkt) found to be associated
with decreased survival, and activation of the pathway, as measured by Akt or mTOR
phosphorylation levels
Editor's Notes
The ovaries produce eggs (called ova).
They are also the main source of a woman’s female hormones, estrogen and
progesterone.
Epithelial ovarian tumors
Epithelial ovarian tumors are further divided into 3 sub-groups: benign, low malignant
potential, and malignant.
Benign epithelial tumors
These tumors are not cancer. They don’t spread and usually do not lead to serious illness.
Tumors of low malignant potential (LMP tumors)
These tumors do not clearly appear to be cancer when looked at under the microscope.
They are also known as borderline epithelial ovarian cancer. They tend to affect women
at a younger age than other ovarian cancers. They grow and spread slowly and are less
life-threatening than most ovarian cancers.
Malignant epithelial ovarian tumors
These are the most common ovarian cancer. When someone says they have ovarian
cancer, they usually mean this kind. These cancers can also be divided into different
types based on certain features.
The information in this document is about the most common kind of ovarian cancer,
invasive epithelial ovarian carcinoma. Be sure to ask your doctor what type of ovarian
cancer you have. If you need information about low malignant potential tumors, ovarian
stromal tumors, or ovarian germ cell tumors, please see our detailed document, Ovarian
Cancer.
If the symptoms suggest ovarian cancer, three tests should be performed:
a complete pelvic exam, including a rectum and vaginal exam;
a vaginal ultrasound; and
a CA-125 blood test