SlideShare a Scribd company logo
Seminario biología molecular.
Juliana Sofia Benavides Guerrero.
Universidad Pontificia Bolivariana.
III semestre.
It is a biological process in which
epithelial cells acquire
characteristics of mesenchymal
cells
Facilitates the ability of cells to
move and spread from the
original tumor site to other
tissues in the body, known as
metastasis.
EMT (EPITHELIAL TO
MESENCHYMAL TRANSITION)
Tumor
microenvironment
Metastasis
Uncontrolled cell
growth
Genetics
Hormones
Growth factors
1
2
3
4
5
6
BREAST CANCER
It is a disease caused by changes in breast cells that
cause uncontrolled cell growth. Some of the main
biological causes of breast cancer include:
This study will focus on 4T1 cells, a
murine mammary
adenocarcinoma cell line, as the
primary research subject. We aim
to investigate the inhibitory
effects and mechanisms of
propranolol on epithelial–
mesenchymal transition (EMT) in
breast cancer cells, aiming to
elucidate this phenomenon at the
miRNA level.
GENERAL OBJECTIVE
1.Sequencing
Second-generation sequencing (SGS)
2.RT‐PCR
It is used to extract, reverse transcribe
and analyze the expression of mRNA and
miRNA. Specific kits were used for
reverse transcription and qPCR
They also used internal controls and
primer sequences for amplification.
METHODS
miRNA and mRNA samples extracted
the sequencing data were processed and
analyzed.
Then, miRNA and mRNA expressions were
compared between the control and
propranolol-treated groups.5
4.Western blots
Total protein was extracted from tumor
cells, protein concentration was analyzed,
and electrophoresis was performed to
separate proteins.
4.Immunohistochemistry
presence of specific antibodies, such as
E-cadherin and vimentin in tumor
tissues. provides information on the
expression and distribution of these
proteins at the cellular level in the
context of the tumor.
METHODS
RESULTS
FIGURE 1 FIGURE 2
RESULTS
FIGURE 3 FIGURE 4
Bowles
(2000)
Singh y
Settleman
(2010)
Wolter (2014)
DISCUSSION
“Sox6 is a member of the
SoxD subfamily and is
involved in organ
development and various
physiological proc”.
Agree
“ When tumor cells undergo
EMT, there is a significant
alteration in intercellular
adhesion, invasion, and
migration abilities, establishing
the fundamental conditions
for distant metas- tasis of
tumor”.
“In recent years there has
been notable progress in
propranolol research in the
field of cancer, with studies
demonstrating its antitumor
effects in neuroblastoma.”.
Agree
Agree
Author What did they say? Agree or
disagree
CONCLUSIONS
2. This study highlights the
importance of combining different
experimental techniques, such as
next-generation sequencing and
molecular validation, to understand
the molecular mechanisms involved
in cancer progression and develop
more effective and specific
treatments.
1. Research in molecular biology
and medicine, such as that carried
out in this study, is essential to
identify new therapeutic
strategies against breast cancer,
such as the use of propranolol to
inhibit epithelial-mesenchymal
transition (EMT) and reduce the
Lung metastasis.
4T1 CELLS AND MURINE MAMMARY
ADENOCARCINOMA
PROPRANOLOL AND ITS
INHIBITORY EFFECT
This was the cell line
that was used in this
study.
Was the type of
cancer investigated
in the study
EPITHELIAL-MESENCHYMAL
TRANSITION (EMT):
MIARN (MICROARN) Y
SOX6
METHODS USED
Its inhibitory effect
on epithelial-
mesenchymal
transition (EMT) is
studied.
Transformation of
epithelial cells into
mesenchymal cells,
allowing cancer
progression.
Identification of
miRNAs involved in
EMT, such as miR-
499-5p.
Identification of
Sox6 as a functional
miR-499-5p gene.
Sequencing
RT‐PCR
Western blots
Immunohistochemistry
This study will focus on 4T1 cells, a murine mammary adenocarcinoma cell line.
The goal is to investigate the inhibitory effects and mechanisms of propranolol on
epithelial-mesenchymal transition (EMT) in breast cancer cells, with the goal of
elucidating . this phenomenon at the miRNA level.
Results and conclusions
1.Inhibition of EMT by propranolol.
2. Identification of miR-499-5p
and Sox6 as key factors.
3. Therapeutic potential of
propranolol in the treatment of
breast cancer.
Propranolol inhibits EMT and metastasis in breast
cancer through miR‐499‐5p‐mediated Sox6

More Related Content

Similar to Seminario biologia molecular-Universidad Pontificia Bolivariana.

2015 City of Hope Summer Research
2015 City of Hope Summer Research 2015 City of Hope Summer Research
2015 City of Hope Summer Research
Joy Cai
 
Biomol
BiomolBiomol
DNA TRANSCRIPTION
DNA TRANSCRIPTION DNA TRANSCRIPTION
DNA TRANSCRIPTION
saratoro20
 
ASCB 2014 poster
ASCB 2014  posterASCB 2014  poster
ASCB 2014 poster
Maxwell Schwam
 
Bohomolets Oncology Lecture year 5
Bohomolets Oncology Lecture year 5Bohomolets Oncology Lecture year 5
Bohomolets Oncology Lecture year 5
Dr. Rubz
 
In vitro and in vivo models of cancer.docx
In vitro and in vivo models of cancer.docxIn vitro and in vivo models of cancer.docx
In vitro and in vivo models of cancer.docx
write4
 
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...
ijtsrd
 
Molecular profiling of breast cancer
Molecular profiling of breast cancerMolecular profiling of breast cancer
Molecular profiling of breast cancer
Hriman Sharma Sarkar
 
Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...
Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...
Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...
karito04_15
 
3Mar15-Ramhold Departmental Seminar (edited)
3Mar15-Ramhold Departmental Seminar (edited)3Mar15-Ramhold Departmental Seminar (edited)
3Mar15-Ramhold Departmental Seminar (edited)
Christopher Ramhold
 
Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...
Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...
Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...
Enrique Moreno Gonzalez
 
Cancer, Etiology, Classification and Pathophysiology
Cancer, Etiology, Classification and PathophysiologyCancer, Etiology, Classification and Pathophysiology
Cancer, Etiology, Classification and Pathophysiology
Himanshu Sharma
 
Seminario Biología Molecular- Valentina Zapata.pdf
Seminario Biología Molecular- Valentina Zapata.pdfSeminario Biología Molecular- Valentina Zapata.pdf
Seminario Biología Molecular- Valentina Zapata.pdf
ValentinaZapata63
 
TUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptx
TUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptxTUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptx
TUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptx
RhythmKarir
 
Chemotherapy of cancer
Chemotherapy of cancerChemotherapy of cancer
Chemotherapy of cancer
balaji college of pharmacy
 
Journal.pmed.1000025
Journal.pmed.1000025Journal.pmed.1000025
Journal.pmed.1000025
Elsa von Licy
 
Suppress lung cancer progression via up regulation of linc rna-p21
Suppress lung cancer progression via up regulation of linc rna-p21Suppress lung cancer progression via up regulation of linc rna-p21
Suppress lung cancer progression via up regulation of linc rna-p21
Clinical Surgery Research Communications
 
Analysis of primary breast tumour stromal cells and their potential role in d...
Analysis of primary breast tumour stromal cells and their potential role in d...Analysis of primary breast tumour stromal cells and their potential role in d...
Analysis of primary breast tumour stromal cells and their potential role in d...
Marion Hartmann
 
Potential cancer cells and therapy to treat melanoma
Potential cancer cells and therapy to treat melanomaPotential cancer cells and therapy to treat melanoma
Potential cancer cells and therapy to treat melanoma
luisa2195
 
Recent developments in p53 and nano oncology
Recent developments in p53 and nano oncologyRecent developments in p53 and nano oncology
Recent developments in p53 and nano oncology
tazib rahaman
 

Similar to Seminario biologia molecular-Universidad Pontificia Bolivariana. (20)

2015 City of Hope Summer Research
2015 City of Hope Summer Research 2015 City of Hope Summer Research
2015 City of Hope Summer Research
 
Biomol
BiomolBiomol
Biomol
 
DNA TRANSCRIPTION
DNA TRANSCRIPTION DNA TRANSCRIPTION
DNA TRANSCRIPTION
 
ASCB 2014 poster
ASCB 2014  posterASCB 2014  poster
ASCB 2014 poster
 
Bohomolets Oncology Lecture year 5
Bohomolets Oncology Lecture year 5Bohomolets Oncology Lecture year 5
Bohomolets Oncology Lecture year 5
 
In vitro and in vivo models of cancer.docx
In vitro and in vivo models of cancer.docxIn vitro and in vivo models of cancer.docx
In vitro and in vivo models of cancer.docx
 
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...
 
Molecular profiling of breast cancer
Molecular profiling of breast cancerMolecular profiling of breast cancer
Molecular profiling of breast cancer
 
Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...
Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...
Level of Tumor Protein Indicates Chances Cancer Will Spread and Structural De...
 
3Mar15-Ramhold Departmental Seminar (edited)
3Mar15-Ramhold Departmental Seminar (edited)3Mar15-Ramhold Departmental Seminar (edited)
3Mar15-Ramhold Departmental Seminar (edited)
 
Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...
Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...
Odontogenic ameloblast-associated protein (ODAM) inhibits growth and migratio...
 
Cancer, Etiology, Classification and Pathophysiology
Cancer, Etiology, Classification and PathophysiologyCancer, Etiology, Classification and Pathophysiology
Cancer, Etiology, Classification and Pathophysiology
 
Seminario Biología Molecular- Valentina Zapata.pdf
Seminario Biología Molecular- Valentina Zapata.pdfSeminario Biología Molecular- Valentina Zapata.pdf
Seminario Biología Molecular- Valentina Zapata.pdf
 
TUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptx
TUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptxTUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptx
TUMOR MICROENVIORNMENT IN HEAD AND NECK CANCER.pptx
 
Chemotherapy of cancer
Chemotherapy of cancerChemotherapy of cancer
Chemotherapy of cancer
 
Journal.pmed.1000025
Journal.pmed.1000025Journal.pmed.1000025
Journal.pmed.1000025
 
Suppress lung cancer progression via up regulation of linc rna-p21
Suppress lung cancer progression via up regulation of linc rna-p21Suppress lung cancer progression via up regulation of linc rna-p21
Suppress lung cancer progression via up regulation of linc rna-p21
 
Analysis of primary breast tumour stromal cells and their potential role in d...
Analysis of primary breast tumour stromal cells and their potential role in d...Analysis of primary breast tumour stromal cells and their potential role in d...
Analysis of primary breast tumour stromal cells and their potential role in d...
 
Potential cancer cells and therapy to treat melanoma
Potential cancer cells and therapy to treat melanomaPotential cancer cells and therapy to treat melanoma
Potential cancer cells and therapy to treat melanoma
 
Recent developments in p53 and nano oncology
Recent developments in p53 and nano oncologyRecent developments in p53 and nano oncology
Recent developments in p53 and nano oncology
 

Recently uploaded

KENT'S REPERTORY by dr niranjan mohanty.pptx
KENT'S REPERTORY by dr niranjan mohanty.pptxKENT'S REPERTORY by dr niranjan mohanty.pptx
KENT'S REPERTORY by dr niranjan mohanty.pptx
SravsPandu1
 
Recent advances on Cervical cancer .pptx
Recent advances on Cervical cancer .pptxRecent advances on Cervical cancer .pptx
Recent advances on Cervical cancer .pptx
DrGirishJHoogar
 
Hemodialysis: Chapter 5, Dialyzers Overview - Dr.Gawad
Hemodialysis: Chapter 5, Dialyzers Overview - Dr.GawadHemodialysis: Chapter 5, Dialyzers Overview - Dr.Gawad
Hemodialysis: Chapter 5, Dialyzers Overview - Dr.Gawad
NephroTube - Dr.Gawad
 
Debunking Nutrition Myths: Separating Fact from Fiction"
Debunking Nutrition Myths: Separating Fact from Fiction"Debunking Nutrition Myths: Separating Fact from Fiction"
Debunking Nutrition Myths: Separating Fact from Fiction"
AlexandraDiaz101
 
Giloy in Ayurveda - Classical Categorization and Synonyms
Giloy in Ayurveda - Classical Categorization and SynonymsGiloy in Ayurveda - Classical Categorization and Synonyms
Giloy in Ayurveda - Classical Categorization and Synonyms
Planet Ayurveda
 
Medical Quiz ( Online Quiz for API Meet 2024 ).pdf
Medical Quiz ( Online Quiz for API Meet 2024 ).pdfMedical Quiz ( Online Quiz for API Meet 2024 ).pdf
Medical Quiz ( Online Quiz for API Meet 2024 ).pdf
Jim Jacob Roy
 
Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)
Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)
Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)
MuskanShingari
 
All about shoulder Joint ..
All about shoulder Joint .. All about shoulder Joint ..
All about shoulder Joint ..
Aswan University Hospital
 
How to Control Your Asthma Tips by gokuldas hospital.
How to Control Your Asthma Tips by gokuldas hospital.How to Control Your Asthma Tips by gokuldas hospital.
How to Control Your Asthma Tips by gokuldas hospital.
Gokuldas Hospital
 
June 2024 Oncology Cartoons By Dr Kanhu Charan Patro
June 2024 Oncology Cartoons By Dr Kanhu Charan PatroJune 2024 Oncology Cartoons By Dr Kanhu Charan Patro
June 2024 Oncology Cartoons By Dr Kanhu Charan Patro
Kanhu Charan
 
Top Travel Vaccinations in Manchester
Top Travel Vaccinations in ManchesterTop Travel Vaccinations in Manchester
Top Travel Vaccinations in Manchester
NX Healthcare
 
RESPIRATORY DISEASES by bhavya kelavadiya
RESPIRATORY DISEASES by bhavya kelavadiyaRESPIRATORY DISEASES by bhavya kelavadiya
RESPIRATORY DISEASES by bhavya kelavadiya
Bhavyakelawadiya
 
Call Girls In Mumbai +91-7426014248 High Profile Call Girl Mumbai
Call Girls In Mumbai +91-7426014248 High Profile Call Girl MumbaiCall Girls In Mumbai +91-7426014248 High Profile Call Girl Mumbai
Call Girls In Mumbai +91-7426014248 High Profile Call Girl Mumbai
Mobile Problem
 
Cervical Disc Arthroplasty ORSI 2024.pptx
Cervical Disc Arthroplasty ORSI 2024.pptxCervical Disc Arthroplasty ORSI 2024.pptx
Cervical Disc Arthroplasty ORSI 2024.pptx
LEFLOT Jean-Louis
 
13. PROM premature rupture of membranes
13.  PROM premature rupture of membranes13.  PROM premature rupture of membranes
13. PROM premature rupture of membranes
TigistuMelak
 
Physical demands in sports - WCSPT Oslo 2024
Physical demands in sports - WCSPT Oslo 2024Physical demands in sports - WCSPT Oslo 2024
Physical demands in sports - WCSPT Oslo 2024
Torstein Dalen-Lorentsen
 
Skin Diseases That Happen During Summer.
 Skin Diseases That Happen During Summer. Skin Diseases That Happen During Summer.
Skin Diseases That Happen During Summer.
Gokuldas Hospital
 
biomechanics of running. Dr.dhwani.pptx
biomechanics of running.   Dr.dhwani.pptxbiomechanics of running.   Dr.dhwani.pptx
biomechanics of running. Dr.dhwani.pptx
Dr. Dhwani kawedia
 
Introduction to British pharmacopeia.pptx
Introduction to British pharmacopeia.pptxIntroduction to British pharmacopeia.pptx
Introduction to British pharmacopeia.pptx
taiba qazi
 
District Residency Programme (DRP) for PGs in India.pptx
District Residency Programme (DRP) for PGs in India.pptxDistrict Residency Programme (DRP) for PGs in India.pptx
District Residency Programme (DRP) for PGs in India.pptx
CommunityMedicine46
 

Recently uploaded (20)

KENT'S REPERTORY by dr niranjan mohanty.pptx
KENT'S REPERTORY by dr niranjan mohanty.pptxKENT'S REPERTORY by dr niranjan mohanty.pptx
KENT'S REPERTORY by dr niranjan mohanty.pptx
 
Recent advances on Cervical cancer .pptx
Recent advances on Cervical cancer .pptxRecent advances on Cervical cancer .pptx
Recent advances on Cervical cancer .pptx
 
Hemodialysis: Chapter 5, Dialyzers Overview - Dr.Gawad
Hemodialysis: Chapter 5, Dialyzers Overview - Dr.GawadHemodialysis: Chapter 5, Dialyzers Overview - Dr.Gawad
Hemodialysis: Chapter 5, Dialyzers Overview - Dr.Gawad
 
Debunking Nutrition Myths: Separating Fact from Fiction"
Debunking Nutrition Myths: Separating Fact from Fiction"Debunking Nutrition Myths: Separating Fact from Fiction"
Debunking Nutrition Myths: Separating Fact from Fiction"
 
Giloy in Ayurveda - Classical Categorization and Synonyms
Giloy in Ayurveda - Classical Categorization and SynonymsGiloy in Ayurveda - Classical Categorization and Synonyms
Giloy in Ayurveda - Classical Categorization and Synonyms
 
Medical Quiz ( Online Quiz for API Meet 2024 ).pdf
Medical Quiz ( Online Quiz for API Meet 2024 ).pdfMedical Quiz ( Online Quiz for API Meet 2024 ).pdf
Medical Quiz ( Online Quiz for API Meet 2024 ).pdf
 
Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)
Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)
Computer in pharmaceutical research and development-Mpharm(Pharmaceutics)
 
All about shoulder Joint ..
All about shoulder Joint .. All about shoulder Joint ..
All about shoulder Joint ..
 
How to Control Your Asthma Tips by gokuldas hospital.
How to Control Your Asthma Tips by gokuldas hospital.How to Control Your Asthma Tips by gokuldas hospital.
How to Control Your Asthma Tips by gokuldas hospital.
 
June 2024 Oncology Cartoons By Dr Kanhu Charan Patro
June 2024 Oncology Cartoons By Dr Kanhu Charan PatroJune 2024 Oncology Cartoons By Dr Kanhu Charan Patro
June 2024 Oncology Cartoons By Dr Kanhu Charan Patro
 
Top Travel Vaccinations in Manchester
Top Travel Vaccinations in ManchesterTop Travel Vaccinations in Manchester
Top Travel Vaccinations in Manchester
 
RESPIRATORY DISEASES by bhavya kelavadiya
RESPIRATORY DISEASES by bhavya kelavadiyaRESPIRATORY DISEASES by bhavya kelavadiya
RESPIRATORY DISEASES by bhavya kelavadiya
 
Call Girls In Mumbai +91-7426014248 High Profile Call Girl Mumbai
Call Girls In Mumbai +91-7426014248 High Profile Call Girl MumbaiCall Girls In Mumbai +91-7426014248 High Profile Call Girl Mumbai
Call Girls In Mumbai +91-7426014248 High Profile Call Girl Mumbai
 
Cervical Disc Arthroplasty ORSI 2024.pptx
Cervical Disc Arthroplasty ORSI 2024.pptxCervical Disc Arthroplasty ORSI 2024.pptx
Cervical Disc Arthroplasty ORSI 2024.pptx
 
13. PROM premature rupture of membranes
13.  PROM premature rupture of membranes13.  PROM premature rupture of membranes
13. PROM premature rupture of membranes
 
Physical demands in sports - WCSPT Oslo 2024
Physical demands in sports - WCSPT Oslo 2024Physical demands in sports - WCSPT Oslo 2024
Physical demands in sports - WCSPT Oslo 2024
 
Skin Diseases That Happen During Summer.
 Skin Diseases That Happen During Summer. Skin Diseases That Happen During Summer.
Skin Diseases That Happen During Summer.
 
biomechanics of running. Dr.dhwani.pptx
biomechanics of running.   Dr.dhwani.pptxbiomechanics of running.   Dr.dhwani.pptx
biomechanics of running. Dr.dhwani.pptx
 
Introduction to British pharmacopeia.pptx
Introduction to British pharmacopeia.pptxIntroduction to British pharmacopeia.pptx
Introduction to British pharmacopeia.pptx
 
District Residency Programme (DRP) for PGs in India.pptx
District Residency Programme (DRP) for PGs in India.pptxDistrict Residency Programme (DRP) for PGs in India.pptx
District Residency Programme (DRP) for PGs in India.pptx
 

Seminario biologia molecular-Universidad Pontificia Bolivariana.

  • 1. Seminario biología molecular. Juliana Sofia Benavides Guerrero. Universidad Pontificia Bolivariana. III semestre.
  • 2. It is a biological process in which epithelial cells acquire characteristics of mesenchymal cells Facilitates the ability of cells to move and spread from the original tumor site to other tissues in the body, known as metastasis. EMT (EPITHELIAL TO MESENCHYMAL TRANSITION)
  • 3. Tumor microenvironment Metastasis Uncontrolled cell growth Genetics Hormones Growth factors 1 2 3 4 5 6 BREAST CANCER It is a disease caused by changes in breast cells that cause uncontrolled cell growth. Some of the main biological causes of breast cancer include:
  • 4. This study will focus on 4T1 cells, a murine mammary adenocarcinoma cell line, as the primary research subject. We aim to investigate the inhibitory effects and mechanisms of propranolol on epithelial– mesenchymal transition (EMT) in breast cancer cells, aiming to elucidate this phenomenon at the miRNA level. GENERAL OBJECTIVE
  • 5. 1.Sequencing Second-generation sequencing (SGS) 2.RT‐PCR It is used to extract, reverse transcribe and analyze the expression of mRNA and miRNA. Specific kits were used for reverse transcription and qPCR They also used internal controls and primer sequences for amplification. METHODS miRNA and mRNA samples extracted the sequencing data were processed and analyzed. Then, miRNA and mRNA expressions were compared between the control and propranolol-treated groups.5
  • 6. 4.Western blots Total protein was extracted from tumor cells, protein concentration was analyzed, and electrophoresis was performed to separate proteins. 4.Immunohistochemistry presence of specific antibodies, such as E-cadherin and vimentin in tumor tissues. provides information on the expression and distribution of these proteins at the cellular level in the context of the tumor. METHODS
  • 9. Bowles (2000) Singh y Settleman (2010) Wolter (2014) DISCUSSION “Sox6 is a member of the SoxD subfamily and is involved in organ development and various physiological proc”. Agree “ When tumor cells undergo EMT, there is a significant alteration in intercellular adhesion, invasion, and migration abilities, establishing the fundamental conditions for distant metas- tasis of tumor”. “In recent years there has been notable progress in propranolol research in the field of cancer, with studies demonstrating its antitumor effects in neuroblastoma.”. Agree Agree Author What did they say? Agree or disagree
  • 10. CONCLUSIONS 2. This study highlights the importance of combining different experimental techniques, such as next-generation sequencing and molecular validation, to understand the molecular mechanisms involved in cancer progression and develop more effective and specific treatments. 1. Research in molecular biology and medicine, such as that carried out in this study, is essential to identify new therapeutic strategies against breast cancer, such as the use of propranolol to inhibit epithelial-mesenchymal transition (EMT) and reduce the Lung metastasis.
  • 11. 4T1 CELLS AND MURINE MAMMARY ADENOCARCINOMA PROPRANOLOL AND ITS INHIBITORY EFFECT This was the cell line that was used in this study. Was the type of cancer investigated in the study EPITHELIAL-MESENCHYMAL TRANSITION (EMT): MIARN (MICROARN) Y SOX6 METHODS USED Its inhibitory effect on epithelial- mesenchymal transition (EMT) is studied. Transformation of epithelial cells into mesenchymal cells, allowing cancer progression. Identification of miRNAs involved in EMT, such as miR- 499-5p. Identification of Sox6 as a functional miR-499-5p gene. Sequencing RT‐PCR Western blots Immunohistochemistry This study will focus on 4T1 cells, a murine mammary adenocarcinoma cell line. The goal is to investigate the inhibitory effects and mechanisms of propranolol on epithelial-mesenchymal transition (EMT) in breast cancer cells, with the goal of elucidating . this phenomenon at the miRNA level. Results and conclusions 1.Inhibition of EMT by propranolol. 2. Identification of miR-499-5p and Sox6 as key factors. 3. Therapeutic potential of propranolol in the treatment of breast cancer. Propranolol inhibits EMT and metastasis in breast cancer through miR‐499‐5p‐mediated Sox6