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Manuela Álvarez Ramírez
Semestre III
Universidad Pontificia Bolivariana
Alveolar hypercoagulation:
Pathologic state of exaggerated coagulation in the absence of bleeding that happens within the alveoli of
the lung.
Can be associated with:
Respiratory distress syndrome (ARDS).
Pneumonia.
Pulmonary embolism.
Certain systemic inflammatory conditions.
Introduction
Introduction
RUNX1/ NF – kB:
Signaling pathway involving the transcriptor factor RUNX1, and the pathway NF - kB that plays a rol in the regulation of immune
responses.
This pathway is responsible for the immune response as it is a proinflammatory signaling pathway.
Regulates the hypercoagulation and the fibrinolytic inhibition.
General objective
Other studies demonstrated that 6- gingerol
regulated inflammation and metabolism by
inhibiting the NF-κB signaling pathway.
Methods
1.1.6 Immunohistochemisty:
A laboratory method that uses antibodies to check for certain antigens (markers)
in a sample of tissue.
After the antibodies bind to the antigen, the enzyme or dye is activated, and the
antigen can then be seen under a microscope.
Was employed to determine the collagen III expression in lung tissue
2.1.8 Enzyme – linked immunosorbent assay (ELISA):
Is an immunological assay commonly used to measure antiboides, antigens,
proteins and glycoproteins in biological samples
Was used to measure the leves of TF, PAI-1 and trombine.
Methods
2.1.9 Western blot:
Is a technique used to detect a specific protein in blood or tissue sample.
It involves electrophoresis to separate the protein and bring it to the surface of the membrane, this
is exposed to an antibody specific to the target protein.
The antibody is detected using the radioactive or chemica tag.
Was applied to determine TF, PAI-1, P65, p – P65, IKKß and p – IKKß in lung tissue.
2.1.10 Real time quantitative fluorescence PCR (qPCR):
Technique for rapidly producing millions to billions of copies of an specific segment of DNA.
It involves using short sythetic DNA fragments and multiple rounds of DNA synthesis to amplify
that segment.
Was used to analyzed mRNA expressions of TF, PAI – 1 and RUNX1 in lug tissue.
Results
Results
Discussion
Conclusions
01 02
Molecular biology has allowed to
improve diagnostic tools so we can
enable the detection of genetic
mutations, infectious agentes, etc.
This being crucial for developing
targeted therapies and interventions
It has also contributed to build a
more personalized medicine, thanks
to the molecular profiles and specific
disease characteristics, this
approach enhances in treatment
efficacy while minimizing adverse
effects.
Seminario Biología Molecular Manuela Álvarez Ramírez

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Seminario Biología Molecular Manuela Álvarez Ramírez

  • 1. Manuela Álvarez Ramírez Semestre III Universidad Pontificia Bolivariana
  • 2. Alveolar hypercoagulation: Pathologic state of exaggerated coagulation in the absence of bleeding that happens within the alveoli of the lung. Can be associated with: Respiratory distress syndrome (ARDS). Pneumonia. Pulmonary embolism. Certain systemic inflammatory conditions. Introduction
  • 3. Introduction RUNX1/ NF – kB: Signaling pathway involving the transcriptor factor RUNX1, and the pathway NF - kB that plays a rol in the regulation of immune responses. This pathway is responsible for the immune response as it is a proinflammatory signaling pathway. Regulates the hypercoagulation and the fibrinolytic inhibition.
  • 4. General objective Other studies demonstrated that 6- gingerol regulated inflammation and metabolism by inhibiting the NF-κB signaling pathway.
  • 5. Methods 1.1.6 Immunohistochemisty: A laboratory method that uses antibodies to check for certain antigens (markers) in a sample of tissue. After the antibodies bind to the antigen, the enzyme or dye is activated, and the antigen can then be seen under a microscope. Was employed to determine the collagen III expression in lung tissue 2.1.8 Enzyme – linked immunosorbent assay (ELISA): Is an immunological assay commonly used to measure antiboides, antigens, proteins and glycoproteins in biological samples Was used to measure the leves of TF, PAI-1 and trombine.
  • 6. Methods 2.1.9 Western blot: Is a technique used to detect a specific protein in blood or tissue sample. It involves electrophoresis to separate the protein and bring it to the surface of the membrane, this is exposed to an antibody specific to the target protein. The antibody is detected using the radioactive or chemica tag. Was applied to determine TF, PAI-1, P65, p – P65, IKKß and p – IKKß in lung tissue. 2.1.10 Real time quantitative fluorescence PCR (qPCR): Technique for rapidly producing millions to billions of copies of an specific segment of DNA. It involves using short sythetic DNA fragments and multiple rounds of DNA synthesis to amplify that segment. Was used to analyzed mRNA expressions of TF, PAI – 1 and RUNX1 in lug tissue.
  • 10. Conclusions 01 02 Molecular biology has allowed to improve diagnostic tools so we can enable the detection of genetic mutations, infectious agentes, etc. This being crucial for developing targeted therapies and interventions It has also contributed to build a more personalized medicine, thanks to the molecular profiles and specific disease characteristics, this approach enhances in treatment efficacy while minimizing adverse effects.