LC-MS/MS IN DETAILS HOW THE ANLYTE MOLECULE FROM THE SOLUTION TO GASSES PAHSE IN THAT GENERATION OF ION ON CHARGE BASE AND WITH HELP OF ANALYSER HOW IT CAN BE DETECTED UP TO FEMTOGRAM LEVEL AND EVEN MORE.
LC-MS/MS IN DETAILS HOW THE ANLYTE MOLECULE FROM THE SOLUTION TO GASSES PAHSE IN THAT GENERATION OF ION ON CHARGE BASE AND WITH HELP OF ANALYSER HOW IT CAN BE DETECTED UP TO FEMTOGRAM LEVEL AND EVEN MORE.
A tandem mass spectrometry (TANDEM MS), also named as MS/MS, is a two-step technique used to analyze a sample either by using two or more mass spectrometers connected to each other or a single mass spectrometer by several analyzers arranged one after another.
UV-VISIBLE SPECTROSCOPY.
1. Introduction
2. Absorption Law (Beers & Lamberts Law)
3. Theory of Electronic Spectroscopy
1. Types of Electronic Transitions (K, R, B, E Bands)
2. The Chromophore Concept
3. Auxochrome
4. Absorption and Intensity Shifts
a. Bathochromic effect. (Red Shift)
b. Hypsochromic shift (Blue Shift)
c. Hyperchromic effect (Abs Increase)
d. Hypochromic effect (Abs Decrease)
5. Effect of Temperature and Solvent on the Fineness of Absorption Band.
6. Woodward-fieser Rules for Calculating Absorption Maximum in Dienes
7. Instrumentation.
A. Light Source
b. Collimating System
c. Monochromator
d. Sample Holder
e. Detector.
3.1 Introduction
3.2 Principle of infra-red spectroscopy
3.3 Theory—Molecular Vibrations
3.4 Vibrational Frequency
3.5 Number of Fundamental Vibrations
3.6 Selection Rules (Active and Forbidden
Vibrations)
3.7 Factors Influencing Vibrational Frequencies
3.8 Scanning of Infra-red Spectrum (Instrumentation)
3.9 Sampling Techniques
3.10 Finger Print Region
3.11 Spectral Features of Some classes of organic
Compounds
3.11 A1 Alkanes and Alkyl residues
3.11 A2 Alkenes
3.11 A3 Alkynes
3.11 A4 Cycloalkanes
3.11 A5 Aromatic Hydrocarbons
3.11 B Halogen Compounds
3.11 C Alcohols and Phenols
3.11 D Ethers
3.11 E Carbonyl compounds
3.11 E1 Aldehydes and Ketones
3.11 F Esters and Lactones
3.11 G Carboxylic Acids
3.11 H Acid Halides
3.11 I Acid Anhydrides
3.11 J Amides
3.11 K Lactams
3.11 L Amino Acids
The combined technique between MS and HPLC is commonly known as LC-MS. Combining the two analytical methods reduces experimental error and improves accuracy. The application of LC-MS is very useful in situations that involve a huge number of compounds, such as environmental effluents.
HPLC
Chromatography
Mobile Phase & Stationary Phase
CLASSIFICATION OF CHROMATOGRAPHY
Characteristics of HPLC
Purpose
Superiority of HPLC
TYPES OF HPLC TECHNIQYES
Principle
PHASING SYSTEM & (normal vs reversed phase)
INSTRUMENTATION
Flow diagram of HPLC instrument
Advantages of HPLC
Instrumentation of HPLC, principle by kk sahuKAUSHAL SAHU
INTRODUCTION
Instrumentation of HPLC
TYPES OF HPLC
PARAMETERS
APPLICATION
CONCLUSION
REFERENCE
High-performance liquid chromatography ( HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds.
HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules.
A tandem mass spectrometry (TANDEM MS), also named as MS/MS, is a two-step technique used to analyze a sample either by using two or more mass spectrometers connected to each other or a single mass spectrometer by several analyzers arranged one after another.
UV-VISIBLE SPECTROSCOPY.
1. Introduction
2. Absorption Law (Beers & Lamberts Law)
3. Theory of Electronic Spectroscopy
1. Types of Electronic Transitions (K, R, B, E Bands)
2. The Chromophore Concept
3. Auxochrome
4. Absorption and Intensity Shifts
a. Bathochromic effect. (Red Shift)
b. Hypsochromic shift (Blue Shift)
c. Hyperchromic effect (Abs Increase)
d. Hypochromic effect (Abs Decrease)
5. Effect of Temperature and Solvent on the Fineness of Absorption Band.
6. Woodward-fieser Rules for Calculating Absorption Maximum in Dienes
7. Instrumentation.
A. Light Source
b. Collimating System
c. Monochromator
d. Sample Holder
e. Detector.
3.1 Introduction
3.2 Principle of infra-red spectroscopy
3.3 Theory—Molecular Vibrations
3.4 Vibrational Frequency
3.5 Number of Fundamental Vibrations
3.6 Selection Rules (Active and Forbidden
Vibrations)
3.7 Factors Influencing Vibrational Frequencies
3.8 Scanning of Infra-red Spectrum (Instrumentation)
3.9 Sampling Techniques
3.10 Finger Print Region
3.11 Spectral Features of Some classes of organic
Compounds
3.11 A1 Alkanes and Alkyl residues
3.11 A2 Alkenes
3.11 A3 Alkynes
3.11 A4 Cycloalkanes
3.11 A5 Aromatic Hydrocarbons
3.11 B Halogen Compounds
3.11 C Alcohols and Phenols
3.11 D Ethers
3.11 E Carbonyl compounds
3.11 E1 Aldehydes and Ketones
3.11 F Esters and Lactones
3.11 G Carboxylic Acids
3.11 H Acid Halides
3.11 I Acid Anhydrides
3.11 J Amides
3.11 K Lactams
3.11 L Amino Acids
The combined technique between MS and HPLC is commonly known as LC-MS. Combining the two analytical methods reduces experimental error and improves accuracy. The application of LC-MS is very useful in situations that involve a huge number of compounds, such as environmental effluents.
HPLC
Chromatography
Mobile Phase & Stationary Phase
CLASSIFICATION OF CHROMATOGRAPHY
Characteristics of HPLC
Purpose
Superiority of HPLC
TYPES OF HPLC TECHNIQYES
Principle
PHASING SYSTEM & (normal vs reversed phase)
INSTRUMENTATION
Flow diagram of HPLC instrument
Advantages of HPLC
Instrumentation of HPLC, principle by kk sahuKAUSHAL SAHU
INTRODUCTION
Instrumentation of HPLC
TYPES OF HPLC
PARAMETERS
APPLICATION
CONCLUSION
REFERENCE
High-performance liquid chromatography ( HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds.
HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules.
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Objective:
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