how exactly DNA replication take place. this power point presentation will explain you exactly what you want.

1. Prof. vkmBIO DEPARTMENT OF BIOTECHNOLOGY ISMAIL YUSUF COLLEGE BIOTECHNOLOGY

3. Join By PHOSPHODIESTER Bond

4. Running InANTI-PARALLELDirection

5. TWO Chain Joined By HYDROGEN BOND

6. LADDERLike Structure Form

7. Ladder Coil To Form DOUBLE HELIX

8. Each Turn Is CalledSPIRAL/ GYRE/PITCH.

9. Diameter Of DNA Double Helix Is 20 A0.

10. Each Turn Has 10 PAIRS Nucleotidesbiologyvm@gmail.com

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12. COMPONENT OF NUCLEOTIDE biologyvm@gmail.com

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16. DNA Replication Take Place By Semi-conservative Mechanism.

17. One Strand Is New While Another Stand Is Old.

18. 50%OLD and 50% NEW.biologyvm@gmail.com

19. 1. ACTIVATION OF NUCLEOTIDES TRIPHOSPHATES dATP dGTP dTTP dCTP MONOPHOSPHATES dAMP dGMP dTMP dCMP biologyvm@gmail.com

20. 2. RECOGNISATION OF ORIGIN Specific Sites Where DNA Replication Begins. DNA Molecule Is Broken By Activity Of Endonuclease Enzyme At The Origin By Formation Of An Incision (Nick) Prokaryotes Has Single While Eukaryotes Has Multiple Origin. biologyvm@gmail.com

21. 3. UNWINDING OF DNA STRANDS Separation Of Strands Is Done By DNA Unwinding Protein i.e. Helicase Enzyme (Rep Protein) Now DNA Molecule Appears As Inverted ‘Y’-shaped Structure Called Replication Fork. The Separated Strands Are Stabilized By SSBP (Single Strand Binding Protein) Or Helix Destabilizing Protein. biologyvm@gmail.com

23. Require 3’OH Active Group Of The PRIMER (PRIMASE)

24. New Nucleotides Are Added According To COMPLEMENTARY Rule.biologyvm@gmail.com

25. 4. SYNTHESIS OF NEW STRANDS biologyvm@gmail.com

26. 5.LEADING AND LAGGING STRANDS biologyvm@gmail.com

27. 5.LEADING AND LAGGING STRANDS biologyvm@gmail.com

28. DNA REPLICATION ANIMATION biologyvm@gmail.com

29. DNAREPLICATION biologyvm@gmail.com

30. DNAREPLICATION biologyvm@gmail.com

31. DNAREPLICATION biologyvm@gmail.com

32. DNAREPLICATION biologyvm@gmail.com