in this how we will be use harmones for creating male sterility in plant so we can use for hybrid seed production

1. “When Nothing is work, Patience will work”
WELCOME

2. ROLE OF HARMONES IN MALE
STERILITY FOR HYBRID SEED
PRODUCTION
Submitted by :
Rathod Balaji Ulhasita G.
Ph.D. Plant Molecular Biology &
Biotechnology
Dept. of Biotechnology,
J.A.U.
An Assignment presentation
on
Submitted to
Dr. G.K. Kataria
Research Scientist (Pl. Physio),
Cotton Research Station,
College of Agriculture,
J.A.U.
PL PHYSIOL 504 HARMONAL REGULATION OF PLANT
GROWTHAND DEVELOPMENT

3. Outline
1) Introduction
2) What is Male Sterility
3) Types of male sterility
4) Male sterility through hormonal
engineering
5) Conclusion

4. INTRODUCTION
What is Sterility?
 An inability of a living organism to effect sexual reproduction.
 What is Male Sterility?
 It is the failure of plants to produce functional anthers, pollen, or male gametes.
 Occurs mainly in bisexual plants.
 More prevalent than female sterility

5. Feature of Male Sterility
 Absence or malformation of male organs.
 Failure to develop normal microsporogenous tissue anther
 Abnormal microsporogenesis deformed or inviable pollen
 Abnormal pollen maturation
 Non dehiscent anthers but viable pollen, sporophytic control
 Barriers other than incompatibility preventing pollen

6. Why Male Sterility?
 Reduced the cost of hybrid seed production.
 Production of large scale of F1 seeds.
 It avoids enormous manual work of emasculation and
pollination.
 Speedup the hybridization programme.
 Commercial exploitation of hybrid vigour

7. Types of male sterility
• Cytoplasmic male sterility(cms) -governed by cytoplasmic genes
• Genetic male sterility(gms) -governed by nuclear genes
• Cytoplasmic-genetic male sterility(cgms)-governed by both nuclear and
cytoplasmic genes
• Transgenic male streility -induced by the technique of genetic
engneering
• Chemical induced male sterility -induced by the use chemical

8. Male sterility through hormonal
engineering

9. Auxin
Role in floral organ
develepment
Distruption of genes
associated
Auxin transport,
biosynthesis, signaling
Flower abnormalities
5 day at
30/25◦C
Male sterility
Tadashi Sakata et .al 20101
suppressing the transcript level of Yuc2 andYuc6 (encoding auxin
biosynthesis enzymes) which leads to a premature abortion of
microspore development

10. Auxin- Arabidopsis thaliana
7 day 31-35◦C
day/night
arf6/arf8 - shorten petal and stamen filaments, failure to release
pollen, and abnormal ovules

11. Filament
elognation
Pollen
development
Gibberellins
Della protein
Display sterility
phenotype
Abnormal microsporogenesis
and delay growth all floral
organs. Eg stamen ,with
shortened filament that cannot
pollinate pistill
Plackett et. al 2014

12. DELLA PROTEIN – SUPRESS JA SYNTHESIS
DELLA JASMONIC ACID
MYB21/MYB24/
MYB27
Late stamen filament growth in
arabidopsis

13. Jasmonic acid – Arabidopsis thaliana
Growth
Develepment
Fertility
Anther ,stamen and
pollen maturation
JA
Cheng et.al.2011

14. Opr
mutant
JA
Stamen with
abnormal
filament
elongation
Arabidopsis Maize
Opr7/opr8
JA
Feminised tassel devoid stamen
formation , extreme elongnation
of earshanks
JA
Met JA
JMT
MALE
STERILITY
oxo-phytodienoate
reductase genes,
B.napus

15. ABA- RICE
ABA
ABA regulates the expression of tapetum
cell wall bound invertase and
monosaccharide transport genes, leading
to a disturbed carbohydrate
accumulation in the anther
Abiotic stress responses
Male fertility during reproductive
stress
pollen
sterility
Wilson et.al. 2009

16. PYR/PYL –ABA
recepter
ABA
SIGNALING
MALE
STERILITY
Wilson et.al. 2009

17. Brassinosteroid- Arabidopsis T.
BRI1 essential for male fertility in plants
cpd dwf4
reduced filament elongation,
leading to a failure of pollen
delivery to the stigma
failure of
pollen tube
elongation
Yoshida et. al. 2002

18. Significance of Male Sterility in Plant Breeding
Male sterility a primary tool to avoid emasculation
in hybridization.
Hybrid production requires a female plant in which
no viable pollens are borne. Inefficient emasculation
may produce some self fertile progenies.
 Saves lot of time, money and labour.

19. CONCLUSION
 Harmonal engineered male sterility provides tremendous
opportunities to the breeders for enforcing pollination
control in hybrid seed production systems.
 Harmonal engineering will become simpler, faster and
more effective. They will be widely used to promote the
heterosis utilization of vegetables and other crops and play
more and more important roles in plant breeding.