University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania Faculty of Food Science and Technology Department of Food Science, MSc Gastronomy, Nutrition and Dietetics - in the needle of Transylvania fairyland thx to Laura

1. University of Agricultural Sciences and
Veterinary Medicine Cluj-Napoca
San Cristobal de la Laguna, June 27th 2014

2. Location
4833 km from
Universidad
La Laguna

3. Doru PAMFIL
visionary
Rector

4. Domain: Life Sciences
Over 600 staff, 5500 students
Scientific and professional education
Budget:
€ 2.126.820 (gouvernmental resources) +
own resources (up to over € 18.000.000)
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

5. University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Higher Education Institution and Research centre
Two pillars:
–Education: BSc 4y, MSc 2y, PhD 3y
–Research: Institute of Life Sciences
11 research centers, 25 research laboratories

6. Faculties:
–Faculty of Agriculture
–Faculty of Horticulture
–Faculty of Animal Science and Biotechnologies
–Faculty of Veterinary Medicine
–Faculty of Food Science and Technology
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

7. Bachelor Programs (BSc) - 17
–Romanian students + international students
(incoming)
Master Program (MSc) - 16
–Romanian, English, French
PhD Program (collaborations: bilateral diploma
award)
–Romanian, English, French
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

8. Atractive university
• Students from more than 14 countries
• Strongest international orientation
• Dynamic, open to changes and to explore new
education blending methods
• Biggest University for Life Sciences in Romania
• Top publication index in our field in Romania
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

9. Faculty of Food Science and Technology
FOOD
TECHNOLOGY
FOOD
ENGINEERING
SAFETY AND
QUALITY
CONTROL
FOOD
SCIENCE
BIOCHEMISTRY
FOOD
CHEMISTRY
NUTRITION
FOOD
TOXICOLOGY

10. Faculty of Food Science and Technology
VISION
Be the recognized leader in providing integrated
life science solutions for local and international
customers.
MISSION
to produce and disseminate scientific
knowledge
to develop innovations and know-how for the
benefit of society

11. Faculty of Food Science and Technology
CAPABILITIES:
Laboratory of Food Quality and Safety Testing
Laboratory of Food Quality Control and
Adulteration
Laboratory of Molecular Nutrition and
Proteomics

12. Research laboratories
Equipments
GC-FID, LC-MS, HPLC, FT-FIR & UV-VIS Spectrometry
cDNA gene expression,
protein expression profile associated with bioactive compounds

13. Faculty of Food Science and Technology
3 BSc Programs
• Agro-Products Processing Technology
• Food Control and Expertise
• Food Engineering
4 MSc Programs
• Food Quality Management
• Processing Systems and Food Quality Control
• Food Safety and Consumer Protection
• GASTRONOMY, NUTRITION AND DIETETICS (in collaboration
with University of Medicine and Pharmacy of Cluj-Napoca)

14. Disciplines Expertise and Competences Capabilities
Food Science food analysis and
characterisation; food
ingredient interaction;
Analysis of the chemical and
physical properties of
Food, metabolomics
Food Law food regulation International research
expertise
Food Production sustainable food production Novel feeding strategies
Food Consumer consumer attitudes, beliefs and
perceptions of food and
health
Novel quantitative
methodologies
Food Nutrition cellular and molecular nutrition,
nutrigenomics, nutriproteomics
Genomic, transcriptomic,
proteomic platforms
Biosystems
Engineering
food chain integrity, food process
engineering
Traditional processing
equipment for the meat ,
dairy, beer, bread and bakery
industries
Food Safety identification and detection
protocols for a range of food
contaminants and food-borne
Analytical methods,
microbiology methods

15. Pilot plants
Pilot plant for beer and wine
Assist. Prof. dr. eng. Elena Mudura

16. Pilot plants
Pilot plant for bakery products
Assist. Prof. dr. eng. Adriana Paucean

17. Pilot plants
Pilot plant for dairy products
Assist. Prof. dr. eng. Mirela Jimborean

18. Main research topic field
• In vitro, in vivo and in silico study
– Cancer (prostate cancer, breast cancer) –
• conducting studies on treatment/prevention of BAC (animal/vegetal) –
(AxonArray  transcriptomics, microRNA, protein array, antibody array)
 Design of innovative food with bioactive anticancer compounds
(colllaboration with prof H Kloker)
– Food proteins/peptides
• Milk proteins and peptide with antihypertensive effect – design of
innovative yogurt
• Protein and peptide expression, purification and characterisation –
specific milk bioactive peptides and proteins (collaboration with BOKU,
prof D Haltrich)
• Microbiology – developing new biomarkers as bacterial membrane
proteins for food quality and control
– Endemic plants (RT-PCR, proteomics) (prof. Pamfil)
• Human nutrition studies

19. BIOMARKER DISCOVERY AND VALIDATION

20. Motivation  Biomarker Discovery and Validation

21. Overview of types of ‘omic’ biomarkers in terms of the informational complexity of the
models.
Azuaje F et al. Brief Bioinform 2009;10:367-377
© The Author 2009. Published by Oxford University Press. For Permissions, please email:
journals.permissions@oxfordjournals.org

22. IOCN 200822
Microarray experiments  differential gene-expression

23. Simplified workflow of the literature mining system at GlaxoSmithKline.
Agarwal P , Searls D B Brief Bioinform 2008;9:479-492
© The Author 2008. Published by Oxford University Press. For Permissions, please email:
journals.permissions@oxfordjournals.org

24. Omics Matching
Service
(gene&transcripts)-
exp model PCa
Overlap (potential
candidates)
MetaData pre-
processing
Diff Gene-Express
Statistics
Filtering Service
Filtering criteria: statistical
meaningfulness
parametrized & addnotation
(id, FC and p-Value )
List of meaningful genes
diff express in PCa
GO
Biological function
Molecular function
Sub-cellular localization
Filterare (pe
baza datelor
publice)
in silico
Validation
Yes! Where has
this biomarker
been reported?
No! This
gene/transcripts
has not been
reported as
biomarker before
Reported or not (search in
public DBs)? –
YES/NO
Who reported this protein
as biomarker (search
in public DB
-List of genes
(GenBank)
-Link differentially
expressed genes to
specific functional
classes
List of exp. identified
genes Public available
data
(ArrayExpr ess;
GEO;CIBEX)
ANOVA, MatArray, t-test; IPA,
PCA, etc
Computational
pipeline of
candidate
potential BMs
in PCa
Suharoschi, Muntean, Iuga. 2012

34. Protein function prediction using SDS-PAGE and
bioinformatic tools

35. National projects – lifescience
PN-II-RU-TE-2011-3-0154 -
Nanobiotechnology tools for
molecular medicine
applications: Nano- and
microstructures synthesis,
characterisation and in vitro
studies - Cristina Coman, PhD
PN-II-RU-PD-2012-3-0055
Brewer's yeast as biosorbent
for precious and heavy metals
biosorption from waste waters
- Andreea Stanila, PhD

36. Food and Health
Cluj-Napoca inter-university platform
Babes Bolyai University
University of Medicine and
Pharmacy of Cluj-Napoca
Technical University of Cluj-Napoca

39. Colaborations

40. We are on facebook

41. USAMV INTERNATIONAL SYMPOSIUM

42. Contact
Prof. Sevastita MUSTE
DEAN – Faculty of Food Science and Technology
sevastita.muste@usamvcluj.ro
Dr. Ramona SUHAROSCHI
ramona.suharoschi@usamvcluj.ro
3-5 Calea Manastur Str., 400372 Cluj-Napoca, ROMANIA
Mobile:+40 728104320
Tel: 0264-596384
Fax: 0264-593792
www.ingineriealimentara.usamvcluj.ro

43. I hope to see you in Cluj-Napoca
and in our university 