تقدیم به شما خوبان

1. Raman spectroscopy for medical diagnosticsRaman spectroscopy for medical diagnostics
From in-vitro biofluid assays to in-vivo cancerFrom in-vitro biofluid assays to in-vivo cancer
detectiondetection
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2. Raman SpectroscopyRaman Spectroscopy
PrinciplePrinciple
InstrumentInstrument
ApplicationsApplications
First Raman SpectraFirst Raman Spectra
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19301930 – C.V. Raman wins Nobel Prize in Physics– C.V. Raman wins Nobel Prize in Physics
http://www.springerlink.com/content/u4d7aexmjm8pa1fv/fulltext.pdfhttp://www.springerlink.com/content/u4d7aexmjm8pa1fv/fulltext.pdf

3. Raman principleRaman principle
Infrared
(absorption)
Raman
(scattering)
v” = 0
v” = 1
virtual
state
Excitation
Scattered
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www.andor.comwww.andor.com

4. 44

5. Raman SpectrometerRaman Spectrometer
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6. 66

7. 77

8. 88
Diagram of the SERS-based sandwich immunoassay. Antibody-conjugated SERSDiagram of the SERS-based sandwich immunoassay. Antibody-conjugated SERS
tags serve as labels for the biological analyte and are captured bytags serve as labels for the biological analyte and are captured by
superparamagnetic beads which are also functionalized with antibodies specificsuperparamagnetic beads which are also functionalized with antibodies specific
to the analyte. A Raman laser strikes the SERS tags, generating a uniqueto the analyte. A Raman laser strikes the SERS tags, generating a unique
spectrum that easily identifies analytes.spectrum that easily identifies analytes.
Identifying biological agents with surface-enhanced Raman scattering. 18 April 2011, SPIEIdentifying biological agents with surface-enhanced Raman scattering. 18 April 2011, SPIE
Newsroom.DOLNewsroom.DOL

9. In vitro Raman spectroscopyIn vitro Raman spectroscopy
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10. In vivo Raman spectroscopyIn vivo Raman spectroscopy
1010

11. 1111
The handheld contact fiber optic probe for Raman spectroscopy.The handheld contact fiber optic probe for Raman spectroscopy.
Intraoperative brain cancer detection with Raman spectroscopy in humans. ScienceIntraoperative brain cancer detection with Raman spectroscopy in humans. Science
Translational Medicine 11 Feb 2015Translational Medicine 11 Feb 2015

12. Real-time Raman systemReal-time Raman system
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Schematic configuration of the real-time Raman system for skin cancerSchematic configuration of the real-time Raman system for skin cancer
diagnosis.diagnosis.
Real-time Raman Spectroscopy for In Vivo Skin Cancer Diagnosis.Real-time Raman Spectroscopy for In Vivo Skin Cancer Diagnosis. 2012 May 15;72(10):2491-5002012 May 15;72(10):2491-500