This document discusses the importance of quality control for blastocyst transfer in IVF clinics. It emphasizes maintaining equipment like CO2 incubators in proper working condition by regularly checking the CO2 concentration, pH, and temperature using tools like a CO2 analyzer, pH meter, and digital thermometer. Strict quality control is critical for fertilization, embryo growth, and blastocyst conversion rates.
The Embryology laboratory should been designed to provide an environment that is as close to optimum as possible for the growth of human embryos and to provide the best resulting pregnancy rates for patients undergoing IVF cycles.
Here, we discuss what is the components of IVF laboratory.
There are many IVF centers over the globe, but itis time to make criteria, standards and parameters for establishing an IVF center. This talk may help in this
Setting up ART ,IVF laboratory standards -Artificial Reproductive Technique b...Healthcare consultant
The primary function of an ART laboratory is to provide an optimal environment for gametes and embryos. To set up an ART laboratory, that is efficient and safe, the three key areas to focus on would be the place or location of the laboratory, the embryologists, and the protocols or procedures.
The following chapter will focus on the physical layout of the lab, the basic equipment required and consumables used in the ART lab. The responsibilities of the key people in the laboratory, the embryologists and the importance of the procedures and protocols will be elucidated.
The Embryology laboratory should been designed to provide an environment that is as close to optimum as possible for the growth of human embryos and to provide the best resulting pregnancy rates for patients undergoing IVF cycles.
Here, we discuss what is the components of IVF laboratory.
There are many IVF centers over the globe, but itis time to make criteria, standards and parameters for establishing an IVF center. This talk may help in this
Setting up ART ,IVF laboratory standards -Artificial Reproductive Technique b...Healthcare consultant
The primary function of an ART laboratory is to provide an optimal environment for gametes and embryos. To set up an ART laboratory, that is efficient and safe, the three key areas to focus on would be the place or location of the laboratory, the embryologists, and the protocols or procedures.
The following chapter will focus on the physical layout of the lab, the basic equipment required and consumables used in the ART lab. The responsibilities of the key people in the laboratory, the embryologists and the importance of the procedures and protocols will be elucidated.
Air quality: is it that important? And if so, how to measure and control it?Sandro Esteves
Quality and Risk Management in the IVF Laboratory; Redlara Brasil, Belo Horizonte, 14-15 September 2016
Content:
1.Air quality: is it that important?
2. How to control?
3. How to measure?
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
In-vitro fertilization (IVF) is a process by which oocytes are fertilized by sperm outside the women’s womb, in vitro. It still represents one of the most exciting modern scientific developments and continues to have a tremendous impact on
people's lives.
Here, we will discuss all about the embryo development inside the dish.
Also we discuss which embryo to choose for transferring into female's uterus.
ICSI as it is presently performed is far from an ideal solution because the selection of sperm is based on the judgement of an embryologist, who is looking for the most normal appearing sperm available.
Shivani Scientific Industries is dedicated to serve all infertility and fertility clinics with latest ART technology and equipment’s. We provide complete assistance in setting up IVF Lab and equipment’s. The team members of Shivani are always there to help you and give you training in the field of ART technology and in the use of ART equipment’s. You are Welcome to the world of Shivani Scientific where end to end solutions are provided for enabling life through technology at http://www.shivaniivf.com or at http://www.shivaniivf.com/brandwise/brandwise.php?val=36#rgt. You can contact us on 91 22 2896 1768 to inquire about any IVF laboratory equipments and consumables.
Call us to know all about Co2 incubators- co2 meter - co2 incubator price - co2 cell culture - Co2 incubators - co2 incubator principle - use of co2 incubator - incubator co2 - Co2 incubator uses - binder co2 incubator - air jacketed co2 incubator.
Air quality: is it that important? And if so, how to measure and control it?Sandro Esteves
Quality and Risk Management in the IVF Laboratory; Redlara Brasil, Belo Horizonte, 14-15 September 2016
Content:
1.Air quality: is it that important?
2. How to control?
3. How to measure?
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
In-vitro fertilization (IVF) is a process by which oocytes are fertilized by sperm outside the women’s womb, in vitro. It still represents one of the most exciting modern scientific developments and continues to have a tremendous impact on
people's lives.
Here, we will discuss all about the embryo development inside the dish.
Also we discuss which embryo to choose for transferring into female's uterus.
ICSI as it is presently performed is far from an ideal solution because the selection of sperm is based on the judgement of an embryologist, who is looking for the most normal appearing sperm available.
Shivani Scientific Industries is dedicated to serve all infertility and fertility clinics with latest ART technology and equipment’s. We provide complete assistance in setting up IVF Lab and equipment’s. The team members of Shivani are always there to help you and give you training in the field of ART technology and in the use of ART equipment’s. You are Welcome to the world of Shivani Scientific where end to end solutions are provided for enabling life through technology at http://www.shivaniivf.com or at http://www.shivaniivf.com/brandwise/brandwise.php?val=36#rgt. You can contact us on 91 22 2896 1768 to inquire about any IVF laboratory equipments and consumables.
Call us to know all about Co2 incubators- co2 meter - co2 incubator price - co2 cell culture - Co2 incubators - co2 incubator principle - use of co2 incubator - incubator co2 - Co2 incubator uses - binder co2 incubator - air jacketed co2 incubator.
"During milk reception, tankers or cans of milk may be used to transport it to the dairy or milk plant. To ensure a steady supply of milk to the pasteurizing equipment, the milk in these containers needs to be graded, emptied, measured by weight or volume, sampled, and bulked.
Pulsed electric fields PEF is a non-thermal method of food preservation. It not only inactivates bacteria at low temperatures but also affects minimally the nutritional and sensory properties of the food product.
Exploring the Latest Innovations and Breakthroughs in the World of F&B industry, continue reading these topics along with Industry Buzz, weekly highlights, and fun facts in this week's edition of Tech-knowledge."
"The process of evaluating the quality and safety of milk or dairy products by analyzing a sample of the product on a standardized testing platform is referred to as 'Platform test'.
Spray balls are commonly used in Clean-in-Place (CIP) systems in the food and beverage industry to clean and sanitize tanks, pipelines, and other processing equipment.
Learn in details on the topics above in this weeks edition of Tech-knowledge along with F&B industry buzz for the week, weekly highlights and many fun facts of the domain."
NRF 2024 Presentation
Sunday, January 14 2024
Darin Detwiler, Detwiler Consulting Group, LLC
Dean Hornsby, BluLine Solutions, LLC
Rick Schlenker, Logile, Inc.
Christopher Schlichting, Coborn’s Inc.
Ringbio Microbial Count Plate for microbiology fast enumeration in food and beverage samples. These products are ISO and AOAC methods compliant. They have the advantage of easy operation, low cost and simple material requirement.
Calibration and validation ,difference , ph meter and weigh balance calibrati...Divy Patel
Calibration and validation , ph meter and weigh balance calibration SOPs procedure with difference and frequency of doing this hope this will be helpful for you... also comment if needed
Here's a collection of some of my LinkedIn Posts on the Coronavirus Pandemic.
I don't claim to be an expert - but do try to think logically as a doctor, and use my common sense to reason !
How MICE ( Medical Innovation, Creativity , Innovation and Entrepreneurship) labs is helping medical students to think out of the box at JJ Hospital by allowing them to tinker
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
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TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
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Quality control in the IVF Lab
1. BLASTOCYST TRANSFER AND QUALITY CONTROL
Dr. Sai, Chief Embryologist , Malpani Infertility Clinic Pvt. Ltd.
A good IVF Clinic routinely does Blastocyst Transfers for all their patients. In order to ensure we have
enough blastocysts, it’s very important to ensure Good Quality Control .
We need to religiously maintain our equipment in proper working conditions, which is why we check the
CO2 Concentration, pH and Temperature of our CO2 incubators regularly,
We maintain Log books, because an incubator which functions properly is critical as far as fertilization ,
Embryo growth and Blastocyst conversion is concerned. This is why it is very important to check that
that all equipments work properly, as even the slightest of variation can affect the Blastocyst conversion
rate.
Good IVF Clinics have Skilled Embryologists , who use a CO2 analyzer, ph meter and a digital
Thermometer in their Lab, which helps them to keep their Incubators working properly.
Co2 Analyzer
Picture of CO2 incubator
Picture of CO2 Analyzer
2. The Culture Media, which we use in our IVF Lab, needs 6% Co2 concentration to achieve optimum ph of
Media which is 7.2 to 7.4. We make sure the our Incubators work well at 6% co2 by checking the Co2
concentration using the Co2 Analyzer.
Frequency of Co2 Checking
Co2 Concentration can be checked once in a week , and mandatorily after cleaning, decontaminating
and restarting the Incubator.
We also make sure that the we change the Co2 Cylinders once they are half empty, as there are very
high chances of the Co2 cylinder having Impurities at the bottom half of the Cylinder, which can have an
effect on the embryo growth and Embryo Quality.
So it is important that we change the Co2 cylinder as soon as the Pressure gauge drops below half
Thermometer
Digital Thermometer
3. We use a digital Thermometer to Check the temperature of all our equipments, including the incubator
and the surface of all our heated stages. Embryos need to be pampered to keep them happy !
4. We also keep a mercury Thermometer inside the Co2 Incubator which allows us to easily check the
Temperature of the Incubator , without opening the glass door and avoiding the Co2 drop
Digital pH Meter
5. We use a digital ph meter to check the pH of the Culture media. It needs 3 point Calibration before
use.We use 3 buffers to calibrate it , and make sure that it works well before we check the pH of culture
media. The 26.0C refers to room temperature on this screen.
Frequency of ph Checking
We should Check the pH Mandatorily for every new batch of Culture media, and after cleaning and
restarting the Co2 incubator .
IVF labs without their own CO2 Analyzer, pH meter and digital Thermometers, would never be confident
about their Blastocyst Conversion rate. This is because incubators are electronic equipment , and their
CO2 concentration and temperature are bound to vary over time.
This is why it is important to check the CO2 , pH and Temperature of all equipment to get optimum
fertilization and blastocyst conversion rate.
The best bio-assay is to monitor the blastocyst conversion rate, and at Malpani Infertility Clinic we
expect this to be around 30 -50% .