- The document discusses a whole blood qualitative vitamin D test that can detect vitamin D deficiency from a single drop of blood in just 10 minutes. - More than 50% of the urban population is estimated to be vitamin D deficient but current testing methods require large investments, long turnaround times, and are not practical for point-of-care use. - The described vitamin D test provides semi-quantitative results in a simple, one-step process using a small blood sample at room temperature for fast treatment decisions at a low cost per test.

1. The World’s only
WHOLE BLOOD
QUALITATIVE
Vitamin D test

2. Sunlight for
30 minutes
everyday
Adequate to avoid
Vitamin D
deficiency

3. 3

4. Estimated
>50% of Urban population
Vit. D deficient

5. Key benefits

6. Just a drop of blood

7. A drop of
blood is
sufficient to…

8. Hunt down
Vitamin D
Deficiency
In minutes!

9. Simple 1 step assay
One Drop Blood
Seven Drops Buffer
Results in 10 minutes

10. Simple Interpretation

14. Market Overview
> 50 % of urban population – Vit D deficient.
Only large lab chains perform Vit. D test.
Apart from lab chains, very few stand lone labs perform Vit. D test.
High investment required / large rental commitments required to install equipment to perform test.
Awareness of Vit. D deficiency – but no action taken
Typical ToT = 24-72 hours depending on the location.
People don’t realize their deficiency unless they fall sick.

15. • Hospitals
•Orthopedicians
•Pediatrician
•Endocrinologist/
Diabetologist
•Gynecologist
•Oncologist
• Doctors
• Dr.’s assistant (with
little clinical lab
experience)
•Semi quantitative results
•Fast treatment decision
• Ease of use & storage
• Low cost per test
• 1 steps procedure
• 10 mins to results
• 50 uL whole blood
• Room temp. storage
Target
Customer
Users
Key
Requirements
QDx Vit.D
fitment
Target Market

16. Category Advantages Disadvantages
ELISA •Fully quantitative •Work in aqueous envmt. & Vit. D is poorly
soluble in water.
•Poor correlation in values – between & within
assays
•Cannot distinguish bet. Vit D2 and D3
•Refrigeration is required
•No individual packaging
•Slow method
Lateral
flow
•Good correlation since it can properly
distinguish between deficient & sufficient
samples
•Lowest cost
•Easy room temp. storage
•Individual packaging
•Fastest method
•Semi-quantitative
•Cannot distinguish bet. Vit D2 and D3
LC-
MS/MS
Fully quantitative
Can distinguish bet. Vit D2 and D3
Very precise
Small sample volume is sufficient
Faster than HPLC
Costly – investment & cost per test
Requires training
Labor intensive
Indirect Competition
Categories
QDx Vit. D – The only player in this segment
Patented & In-house manufacturing
True POC testing

17. Value proposition
Problem:
• Hidden deficiency – Not recognized
Solution:
• Most effective screening – Instant diagnosis – 10 minutes
• Rapid results in 10 minutes
Problem
• High investment & costly test
Solution:
• Cost of test = 1/3rd of Lab test
• Economic to patient
Effect:
• Proactive treatment – Prevent the complications
• High chances of patient retention
• Virtually painless test