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11/06/11 Any SINE of Chromosome 16? PV92 PCR/Informatics    ( S hort  In terspersed Repetitive  E lement)
Think of your  entire genome as one very fat book of biological data. 11/06/11 Like this...
100 pages total: - 95 pages of  nonsense - 5 pages of useful information
Only 5 pages of your genetic book contain useful information!
11/06/11 Today we are going to look in a  non-coding  region of your chromosome #16 But remember: You got one chromosome #16 from Mom And one chromosome #16 from Dad Mom thinks hers is better.
Alu  repeats: our targets ,[object Object],[object Object],[object Object],[object Object],11/06/11
Evolutionary Significance of PV92  Alu  Inserts ,[object Object],[object Object],[object Object],[object Object],11/06/11
11/06/11 PCR Results ,[object Object],[object Object],[object Object],No insertion: 641 bp 300 bp  Alu  insert 641 bp  Alu insertion: 941 bp  5 ’ 3 ’ Alu Amplified Region
11/06/11 Actual  Alu  PCR Results 941 bp 641 bp - +/- + + - +/-
[object Object],[object Object],11/06/11 To estimate frequency of  Alu  within a population:
11/06/11 InstaGene Extraction Cell membrane Nuclear membrane Heat disrupts membranes InstaGene matrix binds  released cellular Mg ++ Genomic DNA Mg ++ Mg ++ Mg ++ Mg ++ Mg ++ Mg ++

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PV92 PCR Informatics

  • 1. 11/06/11 Any SINE of Chromosome 16? PV92 PCR/Informatics ( S hort In terspersed Repetitive E lement)
  • 2. Think of your entire genome as one very fat book of biological data. 11/06/11 Like this...
  • 3. 100 pages total: - 95 pages of nonsense - 5 pages of useful information
  • 4. Only 5 pages of your genetic book contain useful information!
  • 5. 11/06/11 Today we are going to look in a non-coding region of your chromosome #16 But remember: You got one chromosome #16 from Mom And one chromosome #16 from Dad Mom thinks hers is better.
  • 6.
  • 7.
  • 8.
  • 9. 11/06/11 Actual Alu PCR Results 941 bp 641 bp - +/- + + - +/-
  • 10.
  • 11. 11/06/11 InstaGene Extraction Cell membrane Nuclear membrane Heat disrupts membranes InstaGene matrix binds released cellular Mg ++ Genomic DNA Mg ++ Mg ++ Mg ++ Mg ++ Mg ++ Mg ++