This document lists publications, abstracts, and presentations by Christy Brucken (née Steffen) involving research on growth factors found in pig uterine secretions. Some key findings include the identification of heparin-binding EGF-like growth factor, connective tissue growth factor, and pleiotrophin in uterine fluids. Many publications and presentations from 1992-1998 involved collaboration between Steffen and researchers including Brigstock, Kim, and others on characterizing these growth factors.
64 andrew fire - 6506559 - genetic inhibition by double-stranded rnaMello_Patent_Registry
Andrew Fire, Stephen Kostas, Mary Montgomery, Lisa Timmons, SiQun Xu, Hiroaki Tabara, Samuel E. Driver, Craig C. Mello - Genetic Inhibition by Double-Stranded RNA
64 andrew fire - 6506559 - genetic inhibition by double-stranded rnaMello_Patent_Registry
Andrew Fire, Stephen Kostas, Mary Montgomery, Lisa Timmons, SiQun Xu, Hiroaki Tabara, Samuel E. Driver, Craig C. Mello - Genetic Inhibition by Double-Stranded RNA
A 2015 Congressional amendment precludes Mitochondrial Replacement Therapy (MRT), a life-saving IVF-based procedure that could prevent a plethora of mitochondrial DNA diseases in the U.S. Individuals do not, at present, have access to this technology to prevent the devastating consequences of mitochondrial DNA disease. At the same time, MRT continues to move forward in other countries, such as the UK.
Has the time come to revisit the federal prohibition of this preventive therapy and research?
Expert panelists discussed the future of MRT policy in the U.S., reviewing the latest technological developments, the regulatory barriers, and the ethical challenges affecting the clinical application of MRT.
This event was free and open to the public.
The Nature and The Functional Complexity of Retrograde Signals. Retrograde signaling refers to the fact that chloroplasts and mitochondria utilize specific signaling molecules to convey information on their developmental and physiological states to the nucleus and modulate the expression of nuclear genes accordingly.
October 8, 2015
From the event "Synthetic Biology: Science, Policy, and Ethics."
Sponsored by the Petrie-Flom Center for Health Law Policy, Biotechnology, and Bioethics at Harvard Law School.
For more information, visit our website at http://petrieflom.law.harvard.edu/events/details/synthetic-biology.
Is microbial ecology driven by roaming genes?beiko
Microbial ecology often makes assumptions about the relationship between phylogeny and function, but these assumptions can be invalidated by lateral gene transfer. We need to take a broader view of relationships between genes and genomes in order to make better sense out of microbes.
A 2015 Congressional amendment precludes Mitochondrial Replacement Therapy (MRT), a life-saving IVF-based procedure that could prevent a plethora of mitochondrial DNA diseases in the U.S. Individuals do not, at present, have access to this technology to prevent the devastating consequences of mitochondrial DNA disease. At the same time, MRT continues to move forward in other countries, such as the UK.
Has the time come to revisit the federal prohibition of this preventive therapy and research?
Expert panelists discussed the future of MRT policy in the U.S., reviewing the latest technological developments, the regulatory barriers, and the ethical challenges affecting the clinical application of MRT.
This event was free and open to the public.
The genetic architecture of recombination rate variation in a natural populat...Susan Johnston
Genome-wide association study vs. regional heritability analysis to detect genetic variants underlying individual recombination rate variation in a wild population of Soay sheep.
A 2015 Congressional amendment precludes Mitochondrial Replacement Therapy (MRT), a life-saving IVF-based procedure that could prevent a plethora of mitochondrial DNA diseases in the U.S. Individuals do not, at present, have access to this technology to prevent the devastating consequences of mitochondrial DNA disease. At the same time, MRT continues to move forward in other countries, such as the UK.
Has the time come to revisit the federal prohibition of this preventive therapy and research?
Expert panelists discussed the future of MRT policy in the U.S., reviewing the latest technological developments, the regulatory barriers, and the ethical challenges affecting the clinical application of MRT.
This event was free and open to the public.
The Nature and The Functional Complexity of Retrograde Signals. Retrograde signaling refers to the fact that chloroplasts and mitochondria utilize specific signaling molecules to convey information on their developmental and physiological states to the nucleus and modulate the expression of nuclear genes accordingly.
October 8, 2015
From the event "Synthetic Biology: Science, Policy, and Ethics."
Sponsored by the Petrie-Flom Center for Health Law Policy, Biotechnology, and Bioethics at Harvard Law School.
For more information, visit our website at http://petrieflom.law.harvard.edu/events/details/synthetic-biology.
Is microbial ecology driven by roaming genes?beiko
Microbial ecology often makes assumptions about the relationship between phylogeny and function, but these assumptions can be invalidated by lateral gene transfer. We need to take a broader view of relationships between genes and genomes in order to make better sense out of microbes.
A 2015 Congressional amendment precludes Mitochondrial Replacement Therapy (MRT), a life-saving IVF-based procedure that could prevent a plethora of mitochondrial DNA diseases in the U.S. Individuals do not, at present, have access to this technology to prevent the devastating consequences of mitochondrial DNA disease. At the same time, MRT continues to move forward in other countries, such as the UK.
Has the time come to revisit the federal prohibition of this preventive therapy and research?
Expert panelists discussed the future of MRT policy in the U.S., reviewing the latest technological developments, the regulatory barriers, and the ethical challenges affecting the clinical application of MRT.
This event was free and open to the public.
The genetic architecture of recombination rate variation in a natural populat...Susan Johnston
Genome-wide association study vs. regional heritability analysis to detect genetic variants underlying individual recombination rate variation in a wild population of Soay sheep.
Caenorhabditis elegans is a tiny, free-living nematode found worldwide. Newly hatched larvae are 0.25 millimetres long and adults are 1 millimetre long. Their small size means that the animals are usually observed with either dissecting microscopes, which generally allow up to 100X magnification, or compound microscopes, which allow up to 1000X magnification. Because C. elegans is transparent, individual cells and subcellular details are easily visualized using Nomarski (differential interference contrast, DIC) optics.
C. elegans has a rapid life cycle and exists primarily as a self-fertilizing hermaphrodite, although males arise at a frequency of <0.2%. These features have helped to make C. elegans a powerful model of choice for eukaryotic genetic studies. In addition, because the animal has an invariant number of somatic cells, researchers have been able to track the fate of every cell between fertilization and adulthood in live animals and to generate a complete cell lineage. Researchers have also reconstructed the shape of all C. elegans cells from electron micrographs, including each of the 302 neurons of the adult hermaphrodite. Moreover, because of the invariant wild-type cell lineage and neuroanatomy of C. elegans, mutations that give rise to developmental and behavioural defects are readily identified in genetic screens. Finally, because C. elegans was the first multicellular organism with a complete genome sequence, forward and reverse genetics have led to the molecular identification of many key genes in developmental and cell biological processes.
The experimental strengths and the similarities between the cellular and molecular processes present in C. elegans and other animals across evolutionary time (metabolism, organelle structure and function, gene regulation, protein biology, etc.) have made C. elegans an excellent organism with which to study general metazoan biology. At least 38% of the C. elegans protein-coding genes have predicted orthologs in the human genome, 60-80% of human genes have an ortholog in the C. elegans genome, and 40% of genes known to be associated with human diseases have clear orthologs in the C. elegans genome. Thus, many discoveries in C. elegans have relevance to the study of human health and disease.
1. Publications: Christy Brucken, née Steffen
1. Besner, G.E., Whelton, D., Crissman-Combs, M.A., Steffen, C.L., Kim, G.Y. and
Brigstock, D.R. (1992) Interaction of heparin-binding epidermal growth factor
with the epidermal growth factor receptor: Modulation by heparin, heparinase, or
synthetic heparin-binding HB-EGF peptides.
Growth Factors 7 289-296
2. Brigstock, D.R., Kim G.Y., Steffen, C.L., and Ismail, N.Y. (1994) Control of
embryonic development by uterine growth factors and cytokines. Assisted
Reproductive Technology/Andrology 6 252-263
3. Kim, G.Y., Besner, G.E., Steffen, C.L., McCarthy, D.W., Downing, M.T.,
Luquette, M.H., Abad, M.S. and Brigstock, D.R. (1995) Purification of heparin-
binding EGF-like growth factor from pig uterine luminal flushings and its
production by endometrial tissues. Biol. Reprod.52 561
4. Brigstock, D.R., Kim, G.Y. and Steffen, C.L. (1996) Pig uterine fluid contains
the developmentally-regulated neurotrophic factor, pleiotrophin.
J. Endocrinol. 148 103-111
5. Brigstock, D.R., Kim, G.Y., Steffen, C.L., Liu A., Vegunta, R.K. and Ismail, N.H.
(1996) High molecular mass forms of epidermal growth factor in pig uterine
secretions. J. Reprod. Fertil.108(2) 313-320
6. Brigstock, D.R., Steffen, C.L., Kim, G.Y., Vegunta, R.K. and Harding, P.A.
(1997) Purification and characterization of novel heparin-binding growth factors
in uterine secretory fluids: Identification as heparin-regulated 10,000-Mr forms of
connective tissue growth factor. J. Biol. Chem 272(32) 20275-20282
7. Steffen, C.L., Ball-Mirth, D.K., Harding, P.A., Bhattacharyya, N., Pillai, S.
and Brigstock, D.R. (1998) Characterization of cell-associated and soluble forms
of connective tissue growth factor (CTGF) produced by fibroblast cells in vitro.
Growth Factors-London 15(3) 199-213
8. Ball, D.K., Surveyor, G.A., Diehl, J.R., Steffen, C.L., Uzumcu, M., Mirando,
M.A. and Brigstock, D.R. (1998) Characterization of 16- to 20-Kilodalton (kDa)
connective tissue growth factors (CTGFs) and demonstration of proteolytic
activity for 38-kDa CTGF in pig uterine luminal flushings. Biol. of Reprod. 59
828-835
Published Abstracts:
1. Brigstock, D.R., Steffen, C.L., Kim, G.Y. and Ismail, N.H. (1993) Pig uterine
secretions contain high molecular weight forms of immunoreactive epidermal
growth factor. Biol. Reprod. 48 (Suppl) A532
2. 2. Steffen, C.L., Kim, G.Y. and Brigstock, D.R. (1993) A novel heparin-binding
growth factor in pig uterine secretions. Mol. Biol. Cell.4 (Suppl) A108
3. Brigstock, D.R., Steffen, C.L., Abad, M.S., Besner, G.E. and Kim, G.Y. (1994)
Heparin-binding EGF-like growth factor is a constituent of pig uterine luminal
flushings. Biol. Reprod. 50 (Suppl 1) A332
4. Brigstock, D.R., Kim, G.Y., Vegunta, R.K. and Steffen, C.L. (1996) Discovery
and isolation of a new polypeptide growth factor in the female reproductive tract.
J. Reprod. Fertil. (Suppl) Abstract Series No. 17 A65
5. Brigstock, D.R., Kim, G.Y., Vegunta, R.K. and Steffen, C.L. (1996) Biological
characterization of a new heparin-binding growth factor in pig uterine luminal
flushings. Biol. Reprod. 5 (Suppl 1) A523
6. Brigstock, D.R. and Steffen, C.L. (1996) Characterization of low molecular mass
forms of connective tissue growth factor (CTGF) that are biologically active and
heparin-binding. Mol Biol Cell
Presentations:
1. Kim, G.Y., Steffen, C.L., Vegunta, R.K., Brigstock, D.R. (1992) A novel
heparin-binding growth factor in porcine uterine luminal fluid. Presented at the
13th
Children’s Hospital and Children’s Hospital Research Foundation Research
Forum, Columbus, OH June 5.
Read by Mr. Kim.
2. Brigstock, D.R., Steffen, C.L., Kim, G.Y. (1992) A 55,000-Mr mitogen in pig
uterine flushings. Presented at the Endocrinolgy of Embryo-Endometrial
Interactions satellite symposium of the 9th
International Congress of
Endocrinology. Bordeaux, France. September 6-10.
Presented by Dr. Brigstock.
3. Ismail, N.H., Steffen, C.L., Vegunta, R.K., Kim, G.Y., Besner, G.E., Cooney,
D.R., Brigstock, D.R. (1993) Epidermal growth factor in uterine fluids. 14th
Annual Pediatric Surgery Residents Conference. Montreal, Quebec, Canada.
October 1993. Read by Dr. Ismail.
4. Steffen, C.L., Kim, G.Y., Brigstock, D.R. (1993) A novel heparin-binding
growth factor in pig uterine secretions. 33rd
Annual Meeting of the American
Society for Cell Biology, New Orleans, LA December 11-15, 1993.
Presented by Dr. Brigstock.
5. Ismail, N.H., Steffen, C.L., Vegunta, R.K., Kim, G.Y., Besner, G.E., Cooney,
D.R., Brigstock, D.R. (1994) Epidermal growth factor in uterine fluids. 5th
3. Annual Presidential Symposium, Columbus Surgical Society, Columbus, OH
January 1994. Read by Dr. Ismail.
6. Kim, G.Y., Besner, G.E., Steffen, C.L., McCarthy, D.W., Brigstock, D.R. (1994)
Synthesis of heparin-binding EGF-like growth factor (HB-EGF) by endometrial
tissues and its isolation from uterine secretory fluids. 15th
Annual Children’s
Hospital and Children’s Hospital Research Foundation Research Forum.
Childrens’ Hospital, Columbus, OH June 2-3, 1994.
Read by Mr. Kim.
7. Brigstock, D.R., Steffen, C.L., Abad, M.S., Besner, G.E., Kim, G.Y. (1994)
Heparin-binding EGF-like growth factor is a constituent of pig uterine luminal
flushings. 26th
Annual Meeting of Society for the Study of Reproduction.
University of Michigan, Ann Arbor, MI, July 24-27, 1994. Read by Mr. Kim.
8. Steffen, C.L., Kim, G.Y., Brigstock, D.R. (1995) Uterine secretory fluids contain
the developmentally-regulated neurotrophic, pleiotrophin. 16th
Annual Children’s
Hospital and Children’s Hospital Research Foundation Research Forum.
Children’s Hospital, Columbus, OH, June 1-2, 1995.
Presented by Ms. Steffen.
9. Brigstock, D.R., Kim, G.Y., Vegunta, R.K. and Steffen, C.L. (1996)
Purification and characterization of a novel heparin-binding growth factor in
uterine secretory fluids: Identification as a heparin-regulated 10,000-Mr protein
that is structurally related to connective tissue growth factor. 17th
Annual
Children’s Hospital and Children’s Hospital Research Foundation Research
Forum. Children’s Hospital, Columbus, OH, June 6-7, 1996. Presented by
Dr. Brigstock.
10. Brigstock, D.R., Kim, G.Y., Vegunta, R.K. and Steffen, C.L. (1996)
Discovery and isolation of a new polypeptide growth factor in the female
reproductive tract. Walpole Session, Annual Meeting of the Society for the Study
of Fertility, Nottingham, UK, July 15-17, 1996. Read by Dr. Brigstock.
11. Brigstock, D.R., Kim, G.Y., Vegunta, R.K. and Steffen, C.L. (1996)
Biological characterization of a new heparin-binding growth factor in pig uterine
luminal flushings. 29th
Annual Meeting of the Society for the Study of
Reproduction, London, Ontario, Canada, July 27-30, 1996.
Read by Dr. Brigstock.
12. Brigstock, D.R. and Steffen, C.L. (1996) Characterization of low molecular mass
forms of connective tissue growth factor (CTGF) that are biologically active and
heparin binding. 36th
Annual Meeting of the American Society for Cell Biology
& 6th
International Congress on Cell Biology, San Francisco, CA, December 7-11.
Presented by Dr. Brigstock.
