This document discusses different methods for protein analysis, with a focus on proximate analysis. It provides details on the Kjeldahl and Dumas methods for determining protein content through nitrogen analysis. The Kjeldahl method involves digestion, distillation, and titration steps to convert organic nitrogen into ammonium ions that can be measured. The Dumas method combusts samples to release nitrogen gas that is collected and measured. Both provide the percentage of nitrogen which can then be used to calculate the protein content. Automated instruments now exist using thermal conductivity detectors to simplify and expedite the Dumas method.
These slide notes describe protein analysis methods. In this part of the presentation, both official and non-official methods of analyzing proteins in food samples are combined. However, Kidjeldah Method is also given. Moreover, the principles of each and every method were highly explained and illustrated.
These slide notes describe protein analysis methods. In this part of the presentation, both official and non-official methods of analyzing proteins in food samples are combined. However, Kidjeldah Method is also given. Moreover, the principles of each and every method were highly explained and illustrated.
Carbohydrates || Food Analysis || Pharmaceutical Analysis Department || M.Pha...saimuniswetha1
Hello everyone,
Today's topic Carbohydrates in Food Analysis subject in M.pharmacy(Pharmaceutical Analysis Department) ..Don't forget to see.. please watch it... If you need explanation about Carbohydrates please click below link : https://youtu.be/aI5UnNYgufY
Introduction and principle of glc, hplc
columns of hplc
columns of glc
detectors of glc
detectors of hplc
chromatography
classification of chromatography
gas liquid chromatography
high performance liquid chromatography
The technique of paper electrophoresis is simple and inexpensive and requires only micro quantities of plasma for separation.
The support medium is a filter paper
The electrophoresis apparatus in its simplest form consists of two troughs to contain buffer solution, through which electric current is passed.
Frequently used in isolating proteins, amino acids and oligopeptides.
Carbohydrates || Food Analysis || Pharmaceutical Analysis Department || M.Pha...saimuniswetha1
Hello everyone,
Today's topic Carbohydrates in Food Analysis subject in M.pharmacy(Pharmaceutical Analysis Department) ..Don't forget to see.. please watch it... If you need explanation about Carbohydrates please click below link : https://youtu.be/aI5UnNYgufY
Introduction and principle of glc, hplc
columns of hplc
columns of glc
detectors of glc
detectors of hplc
chromatography
classification of chromatography
gas liquid chromatography
high performance liquid chromatography
The technique of paper electrophoresis is simple and inexpensive and requires only micro quantities of plasma for separation.
The support medium is a filter paper
The electrophoresis apparatus in its simplest form consists of two troughs to contain buffer solution, through which electric current is passed.
Frequently used in isolating proteins, amino acids and oligopeptides.
The most frequently used methods for measuring protein content in foods include the Kjeldahl method, Dumas method, direct measurement methods using UV-spectroscopy and refractive index measurement. Each method has advantages and disadvantages
CH-3. Anaerobic treatment of wastewaterTadviDevarshi
Anaerobic treatment process, Effects of pH, temperature and other parameters on anaerobic treatment, Concept of anaerobic contact process, anaerobic filter, anaerobic fixed film reactor, fluidized bed and expanded bed reactors and up flow anaerobic sludge blanket (UASB) reactor.
Total Nitrogen Determination - Traditional and Modern MethodsKasun Prabhashwara
This slideshow contains a short overview of importance of total nitrogen determination, traditional Kjeldahl method, its improvements and Dumas method of total nitrogen determination.
Separation of CO2 and Moisture from biogas by Adsorption methodMinza Mumtaz
Group of post graduate students have made a system to remaove carbondioxide and moisture from biogas production. It is reusable. Checkout the presentation to get the idea how to prepare it.
For further information can contact.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
2. Protein analysis:
Proteins are macromolecules formed by amino
acids. Proteins are large size molecules (macromolecules),
polymers of structural units called amino acids.
Protein is a very abundant component of all cells. It is very
important for biological functions and cell structure.
It is composed of 20 different kinds of amino acids. 11 none
essential amino acids and 9 are essential.
Food proteins are very complex.
Proteins vary in molecular mass, ranging from approximately
5000 to more than a million Daltons.
Nitrogen is the most distinguish element present in proteins.
Its content in various food proteins ranges from 13.4 to 19.1%
due to the variation in the specific amino acid composition of
proteins.
Generally, proteins rich in basic amino acids contain more
nitrogen.
4. Numerous methods have been developed to measure
protein content. The basic principles of these methods
include:
a) Determinations of nitrogen,
b) Peptide bonds,
c) Aromatic amino acids,
d) Dye-binding capacity,
e) Ultraviolet absorptivity of proteins,
f) Light scattering properties
5. There are several methods of protein analysis
a) Kjeldhal method
b) Dumas method
c) Infrared spectroscopy
d) Biuret method
e) Lowry method
f) Bradford method
g) Bicinchoninic acid (BCA)
h) Ultraviolet absorptivity of proteins,
6. Kjeldhal Method:
In 1883, March 7th John Kjeldhal , A Danish Chemist developed this
method.
Objective:
Qualitative determination of organic as well as inorganic nitrogen in
the substances.
Sample preparation:
1. Solid food grind and pass through 20 mesh size screen.
2. Sample should be homogenous.
Procedure:
Digestion Distillation Titration
8. DIGESTION:
Aim: To convert all nitrogen into ammonium ions.
For decomposition of nitrogen sample the sample (0.01-5g) is
mixed with sulfuric acid at temperatures between 350 and 380ºC.
The higher the temperature used, the faster digestion can be
obtained.
The speed of the digestion can be greatly improved by the addition
of salt and catalysts.
Potassium sulfate is added in order to increase the boiling point
of sulfuric acid and catalysts are added in order to increase the speed
and efficiency of the digestion procedure.
Oxidizing agents (Hydrogen peroxide) can also be added to
improve the speed even further.
Nitrogenous Compound + H2SO4 +CuSO4 + K2SO4 = (NH4)2SO4 + H2O + CO2 +
Other by-products
9. After digestion is completed the sample is allowed to cool to room temperature,
then diluted with water and transferred to the distillation unit.
(Catalyst + salt) Kjeldahl tablets / Missouri catalyst.
The catalysts are composed of more than 97% of a salt which increases the
boiling temperature of the sulfuric acid and 1 - 3% of one type of catalyst or a
mixture of catalysts in order to increase the speed and efficiency of the digestion
procedure.
Typical catalysts are selenium or metal salts of copper , titanium and murcury.
The selection of a particular catalyst depends on
Ecological and toxic aspects
Practically, reaction time or foaming and sputtering.
Selenium-containing catalyst reacts fastest but it is toxic.
Copper containing catalyst is considerably safer for both humans and the
environment but gives a slower digestion process.
An ideal compromise is the mixed catalyst consisting of copper and titanium
sulfate.
In water containg samples there is a problem of foaming and sputtering.
If foaming is the only problem it is better to use 1-3 drops of a proprietary
antifoam emulsion (Silicone antifoaming liquid).
10. DISTILLATION/ Neutralization:
During the distillation step the ammonium ions (NH4+) are converted into ammonia (NH3)
by adding alkali (NaOH). The ammonia (NH3) is transferred into the receiver vessel by
means of steam distillation.
The receiving vessel for the distillate is filled with an absorbing solution in order to capture
the dissolved ammonia gas.
Aqueous boric acid [B(OH)3] of 2-4% concentration. The ammonia is quantitatively
captured by the boric acid solution forming solvated ammonium ions.
Sulfuric acid or hydrochloric acid that captures the ammonia forming solvated ammonium
ions.
11.
12. Reaction with Boric Acid (H3BO3): [Direct titration]
If Boric Acid is receiving solution then the Ammonia produce in distillation
is react with H3BO3 and produce AMMONIUM BORATE (NH4
+H2BO3
-), it
can directly titrated against sulfuric acid or hydrochloric acid (0.01N to
0.5N) by using indicator.s
Indicator Acidic medium Basic medium
Methyl red red yellow
Tashiro,s indicator Purple green
Tashiro,s indicator is a mixture of methylene blue (0.1) and methyl red
(0.3) in methanol or ethanol.
NH3 + H3BO3 = NH4
+H2BO3
-
TITRATION:
The concentration of the captured ammonium ions can be determined using
two types of titrations:
13. ADVANTAGES:
1) Prevent loss of ammonia by volatilization,
2) No need to back titration, too weak acid to interfere further
titration.
14. Reaction with Hydrochloric Acid (HCl)/ Sulfuric acid: [Back
titration]
The ammonia is captured by a, carefully measured excess of a
standardized acid solution in the receiving flask. The excess of acid in
the receiving solution keeps the pH low, and the indicator does not
change color.
NH3 + HCl (in excess) = NH4
+Cl- + HCl (left back)
The excess acid solution is exactly neutralized by a carefully measured
standardized alkaline base solution such as sodium hydroxide. A color
change is produced at the end point of the titration.
Methyl orange: Acid (red), base (Yellow)
HCl + NaOH = NaCl + H2O
15. V ml of X (N) NaOH = V ml of X (N) HCl = V ml of X (N) NH3
16.
17. Numerical:
In the kjeldhal method for estimation of nitrogen
present in biscuit sample, ammonia evolved from 0.75 g of
sample neutralized with 10 mL of 1M H2SO4. What is the
percentage of nitrogen in the sample.
18. Advantages:
1) Applicable to all types of foods
2) Inexpensive (if not using an automated system)
3) Accurate; an official method for crude protein content
4) Has been modified (micro Kjeldahl method) to measure
microgram quantities of proteins
Disadvantages:
1) Measures total organic nitrogen, not just protein nitrogen
2) Time consuming (at least 2 h to complete)
3) Poorer precision than the biuret method
4) Corrosive reagent
19. Dumas Method:
In 1831 Jean Baptiste Dumas developed this method.
Principle:
A known mass of organic compound is heated with excess of copper oxide
in an atmosphere of CO2. Carbon, hydrogen and sulphur (if present) are
oxidized to CO2, H2O and SO2 respectively while nitrogen gas is set free.
Any oxide of nitrogen that may be formed is reduced back to free nitrogen
by passing over a hot reduced copper gauze.
C + 2CuO = CO2 + 2 Cu
2H + CuO = H2O + Cu
Nitrogen + CuO = N2 + small amount of oxides of Nitrogen
oxides of Nitrogen + Cu = CuO + N2
The Nitrogen thus formed is collected over conc. KOH (40%) solution
which absorb all other gasses i.e. CO2, H2O and SO2. The volume of
nitrogen collected is noted and from this the percentage of nitrogen can
be calculated.
21. CO2 generator:
CO2 is needed for this purpose is produced by heating sodium bicarbonate or
magnesium bicarbonate. The gas is perfectly dried by bubbling through conc.
H2SO4 before passing it through the combustion tube.
Combustion tube:
It is a hard glass tube about 90cm long and about 2cm in diameter. It is packed
with
1) A roll of oxidized copper gauze which prevent backward diffusion of gasses
produced during combustion.
2) An accurately weighed quantity of the substance mixed with excess of cupric
oxide
3) Coarse CuO that filled half of the combustion tube and
4) A reduced copper gauze which help to reduce any oxides of nitrogen formed
during combustion back to nitrogen gas.
22. Schiff’s nitro meter:
It consist of a long graduated tube having a reservoir and a tap at the upper end.
It contains about 40% KOH solution. It also has a mercury seal at the bottom
which prevent KOH solution from being sucked back into the combustion tube.
Both CO2 and H2O produced during combustion are absorbed by KOH solution
while N2 is collected over it. The volume of nitrogen is measure after careful
leveling (by making the level of KOH in the nitro meter tube and reservoir same).
If we keep the level of KOH same in Schiff’s nitro meter and reservoir same then
at that time pressure will be 1 atm.
27. Advantages:
1. Requires no hazardous chemicals.
2. Can be accomplished in 3 min.
3. Recent automated instruments can analyze up to 150
samples without attention.
Disadvantages:
1. Expensive equipment is required.
2. Measures total organic and inorganic nitrogen, not just
protein nitrogen.