This document summarizes recent developments in using femtosecond (fs) laser pulses for cell and embryo nanosurgery. Several studies have demonstrated that fs laser pulses can precisely alter cellular and subcellular structures in simple cells and more complex embryos in a nondestructive manner. The ability to manipulate biological systems noninvasively with fs laser pulses makes it a valuable tool for developmental biologists, geneticists, and other researchers. The document reviews how fs laser pulses have been used for nanosurgery applications such as intrachromosomal dissections, cell isolation, cytoskeletal ablation, and surgical ablation of embryos.
Microbial Metagenomics Drives a New CyberinfrastructureLarry Smarr
06.03.03
Invited Talk
School of Biological Sciences
University of California, Irvine
Title: Microbial Metagenomics Drives a New Cyberinfrastructure
Irvine, CA
A Systems Biology Approach to Natural Products ResearchHuda Nazeer
Explains the systems biology approach (holistic approach), its advantages and tools used compared to the reductionist approach in natural products research.
Perturbing The Interactome: Multi-Omics And Personalized Methods For Network ...Marc Santolini
In this talk, I will describe several recently developed methods to study disease perturbations through the lens of network science. First I will present evidence that one can accurately predict perturbation patterns from the topology of biological networks, even when lacking measurements on the kinetic parameters governing the dynamics of these interactions. Using 87 biochemical networks with experimentally measured kinetic parameters, we show that a knowledge of the network topology offers 65% to 80% accuracy in predicting the impact of perturbations. In other words, we can use the increasingly accurate topological models to approximate perturbation patterns, bypassing expensive kinetic constant measurement. These results open new avenues in modeling drug action, and in identifying drug targets relying on the human interactome only.
Then, I will present a novel approach to identify the collective impact of miRNAs in disease. Instead of focusing on the magnitude of miRNA differential expression, here we address the secondary consequences for the interactome. We developed the Impact of Differential Expression Across Layers (IDEAL), a network-based algorithm to prioritize disease-relevant miRNAs based on the central role of their targets in the molecular interactome. This method was used in the context of asthmatic Th2 inflammation and identified five Th2-related miRNAs (mir27b, mir206, mir106b, mir203, and mir23b) whose antagonization led to a sharp reduction of the Th2 phenotype. This result offers novel approaches for therapeutic interventions.
Finally, I will present an investigation of the personalized gene expression responses when inducing hypertrophy and heart failure in 100+ strains of genetically distinct mice from the Hybrid Mouse Diversity Panel (HMDP). I will show that genes whose expression change significantly correlates with the severity of the disease are either up- or down-regulated across strains, and therefore missed by traditional population-wide analyses of differential gene expression. These uncovered personalised genes are enriched in human cardiac disease genes and form a dense co-regulated module strongly interacting with the cardiac hypertrophic signaling network in the human interactome, the set of molecular interactions in the cell. We validate our approach by showing that the knockdown of Hes1, predicted as a strong candidate, induces a dramatic reduction of hypertrophy by 80-90% in neonatal rat ventricular myocytes, demonstrating that individualized approaches are crucial to identify genes underlying complex diseases as well as to develop personalized therapies.
Lecture given for the Data Mining course at Uppsala university in October 2013. The presentation talks about data analysis in the context of genomics, next-generation sequencing, metagenomics etc.
Microbial Metagenomics Drives a New CyberinfrastructureLarry Smarr
06.03.03
Invited Talk
School of Biological Sciences
University of California, Irvine
Title: Microbial Metagenomics Drives a New Cyberinfrastructure
Irvine, CA
A Systems Biology Approach to Natural Products ResearchHuda Nazeer
Explains the systems biology approach (holistic approach), its advantages and tools used compared to the reductionist approach in natural products research.
Perturbing The Interactome: Multi-Omics And Personalized Methods For Network ...Marc Santolini
In this talk, I will describe several recently developed methods to study disease perturbations through the lens of network science. First I will present evidence that one can accurately predict perturbation patterns from the topology of biological networks, even when lacking measurements on the kinetic parameters governing the dynamics of these interactions. Using 87 biochemical networks with experimentally measured kinetic parameters, we show that a knowledge of the network topology offers 65% to 80% accuracy in predicting the impact of perturbations. In other words, we can use the increasingly accurate topological models to approximate perturbation patterns, bypassing expensive kinetic constant measurement. These results open new avenues in modeling drug action, and in identifying drug targets relying on the human interactome only.
Then, I will present a novel approach to identify the collective impact of miRNAs in disease. Instead of focusing on the magnitude of miRNA differential expression, here we address the secondary consequences for the interactome. We developed the Impact of Differential Expression Across Layers (IDEAL), a network-based algorithm to prioritize disease-relevant miRNAs based on the central role of their targets in the molecular interactome. This method was used in the context of asthmatic Th2 inflammation and identified five Th2-related miRNAs (mir27b, mir206, mir106b, mir203, and mir23b) whose antagonization led to a sharp reduction of the Th2 phenotype. This result offers novel approaches for therapeutic interventions.
Finally, I will present an investigation of the personalized gene expression responses when inducing hypertrophy and heart failure in 100+ strains of genetically distinct mice from the Hybrid Mouse Diversity Panel (HMDP). I will show that genes whose expression change significantly correlates with the severity of the disease are either up- or down-regulated across strains, and therefore missed by traditional population-wide analyses of differential gene expression. These uncovered personalised genes are enriched in human cardiac disease genes and form a dense co-regulated module strongly interacting with the cardiac hypertrophic signaling network in the human interactome, the set of molecular interactions in the cell. We validate our approach by showing that the knockdown of Hes1, predicted as a strong candidate, induces a dramatic reduction of hypertrophy by 80-90% in neonatal rat ventricular myocytes, demonstrating that individualized approaches are crucial to identify genes underlying complex diseases as well as to develop personalized therapies.
Lecture given for the Data Mining course at Uppsala university in October 2013. The presentation talks about data analysis in the context of genomics, next-generation sequencing, metagenomics etc.
Creating a Cyberinfrastructure for Advanced Marine Microbial Ecology Research...Larry Smarr
06.06.27
Invited Talk
ONR Review
Scripps Institution of Oceanography, UCSD
Title: Creating a Cyberinfrastructure for Advanced Marine Microbial Ecology Research and Analysis (CAMERA)—Taking Metagenomics to Light Speed
La Jolla, CA
Lecture presented by Dr.Fatma Taha at BIOCHEM Cairo 2014 organized by Department of Medical Biochemistry and Molecular Biology, Cairo University. BIOCHEM Cairo 2014 is a Scribe event ( www.scribeofegypt.com)
Human organoid are miniature sized, self-organized structures, that are derived from stem cells or tissues in culture. The progress, potential, limitations and challenges are discussed.
Neuromics' is a recognized leader in providing Large Pharma, Biotech, and Academic/Government Labs 2 and 3-D cell-based assays. They are excellent for use in Drug Discover and Toxicology Studies.
Viral Metagenomics (CABBIO 20150629 Buenos Aires)bedutilh
This is a one-hour lecture about metagenomics, focusing on discovery of viruses and unknown sequence elements. It is part of a one-day workshop about metagenome assembly of crAssphage, a bacteriophage virus found in human gut. The hands-on workflow can be found at http://tbb.bio.uu.nl/dutilh/CABBIO/ and should be doable in one afternoon with supervision. There is also an iPython notebook about this here: https://github.com/linsalrob/CrAPy
Microfluidics and organ on a chip technology is an interdisciplinary field of medical and engineering. It will replace the current methods of testing efficacy of drug viz. cells in dishes test and animal testing.
Maria A. Diroma – MEWAs: sviluppo di un sistema bioinformatico per studi di a...eventi-ITBbari
MEWAs (Mitochondriome-Exome Wide Associations): sviluppo di un sistema bioinformatico per studi di associazione fra l’intero esoma nucleare e il DNA mitocondriale in fenotipi fisiologici o patologici.
Building on the sell-out success of the launch event, SMi Group is delighted to announce the return of 3D Cell Culture, taking place on 21st and 22nd of February 2018, in London UK.
3D Cell Culture is rapidly growing with incredible potential for industrial application and a widespread reach that can be seen across many different fields, such as 3D bioprinting and microfluidics.
The 2nd annual conference will explore these overlapping areas and will combine pioneering breakthroughs with scientific research to strengthen your commercial success. Join us for exclusive insight into key topics such as disease models, organoids, organ-on-a-chip technologies, Ipsc advances and CRISPR technology. Notable speakers on the agenda for 2018 will include experts from Aurelia Bioscience, ReInnervate Ltd, Cell and Gene Therapy Catapult, University College London, Novartis Institutes for Biomedical Research, Kugelmeiers, GSK, AstraZeneca, Roche and more!
Aprenda como comprar uma franquia, se você quiser o artigo desse Power Point, segue o link desse artigo na integra:
>> http://franquiasrentaveis.com/como-comprar-uma-franquia/
Creating a Cyberinfrastructure for Advanced Marine Microbial Ecology Research...Larry Smarr
06.06.27
Invited Talk
ONR Review
Scripps Institution of Oceanography, UCSD
Title: Creating a Cyberinfrastructure for Advanced Marine Microbial Ecology Research and Analysis (CAMERA)—Taking Metagenomics to Light Speed
La Jolla, CA
Lecture presented by Dr.Fatma Taha at BIOCHEM Cairo 2014 organized by Department of Medical Biochemistry and Molecular Biology, Cairo University. BIOCHEM Cairo 2014 is a Scribe event ( www.scribeofegypt.com)
Human organoid are miniature sized, self-organized structures, that are derived from stem cells or tissues in culture. The progress, potential, limitations and challenges are discussed.
Neuromics' is a recognized leader in providing Large Pharma, Biotech, and Academic/Government Labs 2 and 3-D cell-based assays. They are excellent for use in Drug Discover and Toxicology Studies.
Viral Metagenomics (CABBIO 20150629 Buenos Aires)bedutilh
This is a one-hour lecture about metagenomics, focusing on discovery of viruses and unknown sequence elements. It is part of a one-day workshop about metagenome assembly of crAssphage, a bacteriophage virus found in human gut. The hands-on workflow can be found at http://tbb.bio.uu.nl/dutilh/CABBIO/ and should be doable in one afternoon with supervision. There is also an iPython notebook about this here: https://github.com/linsalrob/CrAPy
Microfluidics and organ on a chip technology is an interdisciplinary field of medical and engineering. It will replace the current methods of testing efficacy of drug viz. cells in dishes test and animal testing.
Maria A. Diroma – MEWAs: sviluppo di un sistema bioinformatico per studi di a...eventi-ITBbari
MEWAs (Mitochondriome-Exome Wide Associations): sviluppo di un sistema bioinformatico per studi di associazione fra l’intero esoma nucleare e il DNA mitocondriale in fenotipi fisiologici o patologici.
Building on the sell-out success of the launch event, SMi Group is delighted to announce the return of 3D Cell Culture, taking place on 21st and 22nd of February 2018, in London UK.
3D Cell Culture is rapidly growing with incredible potential for industrial application and a widespread reach that can be seen across many different fields, such as 3D bioprinting and microfluidics.
The 2nd annual conference will explore these overlapping areas and will combine pioneering breakthroughs with scientific research to strengthen your commercial success. Join us for exclusive insight into key topics such as disease models, organoids, organ-on-a-chip technologies, Ipsc advances and CRISPR technology. Notable speakers on the agenda for 2018 will include experts from Aurelia Bioscience, ReInnervate Ltd, Cell and Gene Therapy Catapult, University College London, Novartis Institutes for Biomedical Research, Kugelmeiers, GSK, AstraZeneca, Roche and more!
Aprenda como comprar uma franquia, se você quiser o artigo desse Power Point, segue o link desse artigo na integra:
>> http://franquiasrentaveis.com/como-comprar-uma-franquia/
L’innovazione viene definita come un’idea, un prodotto o una tecnologia, sviluppata e successivamente commercializzata presso i consumatori che la percepiscono come nuova.
L’innovazione dunque viene intesa come un processo di identificazione, creazione e distribuzione del valore attraverso un prodotto che in precedenza non esisteva sul mercato e non riguarda la creazione di valore tramite semplici strategie di marketing (es. un cambio di posizionamento).
In questa fase dunque faremo riferimento alle metodologie in base alle quali le imprese creano e commercializzano (a) prodotti nuovi in assoluto, (b) miglioramenti e modifiche di prodotti esistenti, (c) nuove marche create attraverso attività di R&S, mentre non faremo riferimento a nuovi prodotti realizzati tramite acquisizione di imprese e brevetti
Method for physiologic phenotype characterization at the single-cell level in...Shashaanka Ashili
Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measurement platform that features the capability to quantify oxygen consumption rates of individual, non-interacting and interacting cells
under normoxic and hypoxic conditions. It is based on real-time concentration measurements of metabolites of
interest by means of extracellular optical sensors in cell-isolating microwells of subnanoliter volume. We present the results of a series of measurements of oxygen consumption rates (OCRs) of individual non-interacting and interacting human epithelial cells. We measured the effects of cell-to-cell interactions by using the system’s capability to isolate two and three cells in a single well. The major advantages of the approach are: 1. ratiometric, intensity-based characterization of the metabolic phenotype at the single-cell level, 2. minimal invasiveness due to the distant positioning of sensors, and 3. ability to study the effects of cell-cell interactions on cellular respiration rates.
A statistical framework for multiparameter analysis at the single cell levelShashaanka Ashili
Phenotypic characterization of individual cells provides crucial insights into intercellular heterogeneity and enables access to information that is unavailable from ensemble averaged, bulk cell analyses. Single-cell studies have attracted significant interest in recent years and spurred the development of a variety of commercially available and research-grade technologies. To quantify cell-to-cell variability of cell populations, we have developed an experimental platform for real-time measurements of oxygen consumption (OC) kinetics at the single-cell level. Unique challenges inherent to these single-cell measurements arise, and no existing data analysis
methodology is available to address them. Here we present a data processing and analysis method that addresses challenges encountered with this unique type of data in order to extract biologically relevant information. We applied the method to analyze OC profiles obtained with single cells of two different cell lines derived from metaplastic and dysplastic human Barrett’s esophageal epithelium. In terms of method development, three main challenges were considered for this heterogeneous dynamic system: (i) high levels of noise, (ii) the lack of a priori knowledge of single-cell dynamics, and (iii) the role of intercellular variability within and across cell types.
Several strategies and solutions to address each of these three challenges are presented. The features such as slopes, intercepts, breakpoint or change-point were extracted for every OC profile and compared across individual cells and cell types. The results demonstrated that the extracted features facilitated exposition of subtle differences between individual cells and their responses to
cell–cell interactions. With minor modifications, this method can be used to process and analyze
data from other acquisition and experimental modalities at the single-cell level, providing a valuable statistical framework for single-cell analysis.
Journal of Stem Cells Research, Reviews & Reports is a peer-reviewed, open access journal published by Austin Publishers. It provides easy access to high quality Manuscripts in all related aspects covering Stem cell research that focuses on stem cells, which have a capacity to regenerate and develop into other types of cells namely, like kidney cells, liver cells, heart cells, etc. These circulate and function to replace dysfunctional cells, naturally maintaining optimal health. The Journal encourages all the current medical research that is focused on two particular types of stem cells -- adult and embryonic stem cells that are used in various stem cell therapies against many dreadful diseases.
Austin Publishing Group is a successful host of more than hundred peer reviewed, open access journals in various fields of science and medicine with intent to bridge the gap between academia and research access.
Journal of Stem Cells Research, Reviews & Reports accepts original research articles, review articles, case reports, mini reviews, rapid communication, opinions and editorials on all the related aspects of Stem Cells and Cell-Based Therapies.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Rotator cuff repair using a stem cell approachZakary Bondy
This presentation communicates current methods for rotator cuff repair mainly focusing on mesenchymal and tendon-derived stem cells. It looks to expand on future research in this field by communicating a future experiment to expand on current knowledge of tendon-derived stem cells.
Epi-Fluorescence Microscopy: Explore Its Amazing Powers and Uses | The Lifesc...The Lifesciences Magazine
Epi-fluorescence microscopy, also known as epifluorescence microscopy, is a specialized imaging technique that utilizes fluorescence to illuminate specimens of interest.
A seminar report on the chemical frontiers of living matter seminar series - ...Glen Carter
This seminar report highlights a select few presentations of cutting-edge research being done in various labs across the Paris Science et Lettre (PSL) network.
Microdialysis is an integral part of preclinical research to determine extracellular fluid and blood concentrations of metabolites, hormones, drugs, etc, and is often used in quantifying the biochemistry of brain and peripheral tissues. However, it is a molecular-only technique and other imaging modalities are needed to provide the researcher with functional and anatomical information of the animal in vivo.
CHI's Targeting Stromal Cells in Cancer and Inflammatory Diseases Conference ...James Prudhomme
This virtual meeting will highlight cutting-edge science and provide insight into recent developments towards therapeutic stromal cell targeting in cancer and chronic inflammatory diseases. View full details and register: https://www.healthtech.com/stroma-conference
This presentation was created by Ioanna Leontiou and it is intended as a creative and flexible tool for students on Biological sciences who focus on the chromosome segregation. It is created to facilitate students performing research projects in our lab (especially during Covid restrictions), but it is suitable for every student who wants to learn more about chromosomes and the molecular mechanism controlling chromosome segregation. The presentation includes a generic overview of the cell division, illustrates the chromosome structure and provides molecular details of the spindle assembly checkpoint, an important pathway that ensures high fedility of chromosome segregation through mitosis. It also includes an introduction to some of the molecular biology techniques used in a yeast lab and incoporates some fluorescent microscopy images/videos. At the end of the presentantion there is a list of open access scientific publications for further reading on the the molecular mechanism of spindle checkpoint and some links of some very interesting sites, which include a range of videos on laboratory molecular biology techniques, research talks and guided papers. The purpose of this presentantion is to create a piece of work that students could return to when needed. Diagramms and illustrations are also encouranged to be used by scientists, science communicators and educators.
This presentation is licensed under a Creative Common Attribution-ShareAlike 4.0 (CC BY-SA 4.0), unless otherwise stated on the specific slide.
Similar to Prospects and Development (2008) - Kohli et al (20)