This study examined the effects of cooperative learning with initial knowledge compared to cooperative learning without initial knowledge on student learning outcomes in colloid systems. The study involved two 11th grade classes (n=30 each) in SMAN 3 Binjai high school. Students in the experimental class received initial knowledge before cooperative learning, while the control class did not. Post-test scores were higher on average for the experimental class (87) than the control class (82), and hypothesis testing found the difference to be statistically significant, indicating initial knowledge improved learning when used with cooperative learning.