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BIOCHEMICAL PROFILING IN WILD SPECIES OF
CYMBOPOGON AND STUDIES ON THEIR BIOLOGICAL
ACTIVITY.
Under the guidance of,
Kiran J
INTRODUCTION
 Cymbopogon commonly known as lemongrass
belongings to the family Poaceae. It is commonly
used as spice in Indonesia, abundantly found in
Philippines.
 C.citratus commonly known as oil grass, is a
tropical plant. Its fragrant leaves are traditionally
used in cooking.
 C.martinii is a species of lemongrass native to India
and Indochina, widely cultivated for its aromatic oil.
 Lemongrass contains several important bioactive
compounds which are useful in several health
issue.
 C.citratus contains phytochemicals are mainly
terpanes, alcohols, ketones, aldehydes, and esters.
 The Phenolic compounds acts as antioxidants,
which are used to initially to preserve fats and oils.
These are used both in the field of health and food.
 Lemongrass extract to be used as natural
antimicrobial agents against pathogenic bacteria.
OBJECTIVES
 To explore and investigate the wild land races of Cymbopogon
namely, Cymbopogon martini and Cymbopogon citratus.
 To study the biological activities of the crude extract for
antioxidant and antibacterial activity.
METHODOLOGY
 COLLECTION OF PLANT MATERIAL
 PREPARATION OF EXTRACT
 PHYTOCHEMICAL ANALYSIS
 TLC –Gas Chromatography-Mass Spectroscope
 ANTIOXIDENT ASSAY
A. 2,2-Diphenyl-1-picrylhydrazyl radical assay
B. Reducing power
C. Nitric oxide radical scavenging assay.
 Antimicrobial activity
TLC under visible and uv light respectively
Phyto-chemical analysis
GC-MS OF C.citratus
DPPH ASSAY
Reducing power
Nitric oxide scavenging assay
Antimicrobial activity
SUMMARY
CONCLUSION
THANK YOU

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Project ppt

  • 1. BIOCHEMICAL PROFILING IN WILD SPECIES OF CYMBOPOGON AND STUDIES ON THEIR BIOLOGICAL ACTIVITY. Under the guidance of, Kiran J
  • 2. INTRODUCTION  Cymbopogon commonly known as lemongrass belongings to the family Poaceae. It is commonly used as spice in Indonesia, abundantly found in Philippines.  C.citratus commonly known as oil grass, is a tropical plant. Its fragrant leaves are traditionally used in cooking.  C.martinii is a species of lemongrass native to India and Indochina, widely cultivated for its aromatic oil.  Lemongrass contains several important bioactive compounds which are useful in several health issue.
  • 3.  C.citratus contains phytochemicals are mainly terpanes, alcohols, ketones, aldehydes, and esters.  The Phenolic compounds acts as antioxidants, which are used to initially to preserve fats and oils. These are used both in the field of health and food.  Lemongrass extract to be used as natural antimicrobial agents against pathogenic bacteria.
  • 4. OBJECTIVES  To explore and investigate the wild land races of Cymbopogon namely, Cymbopogon martini and Cymbopogon citratus.  To study the biological activities of the crude extract for antioxidant and antibacterial activity.
  • 5. METHODOLOGY  COLLECTION OF PLANT MATERIAL  PREPARATION OF EXTRACT  PHYTOCHEMICAL ANALYSIS  TLC –Gas Chromatography-Mass Spectroscope  ANTIOXIDENT ASSAY A. 2,2-Diphenyl-1-picrylhydrazyl radical assay B. Reducing power C. Nitric oxide radical scavenging assay.  Antimicrobial activity
  • 6. TLC under visible and uv light respectively Phyto-chemical analysis
  • 8. Reducing power Nitric oxide scavenging assay