How to make a blood smear briefly.

4. • Using a special centrifuge (e.g. Cytospin); the blood spreads
on the slide in a mono-layer.

5. • Romanowsky Stains:
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


Leishman’s Stain
May-Grunwald-Giemsa Stain
Jenner’s Stain
Wright’s Stain
• Principle?
Acidic elements in the cell (e.g. nucleus proteins and nucleic
acids of cell nucleus) + Basic dye (Azure-B)
Blue
Basic elements such as (Hb) + Acidic dye (Eosin-Y)
Red

6. • Requirements:
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



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Leishman’s Stain
Leishman’s Buffer solution
Staining Rack
Methanol (fixation)
D.W.
Pipette and cotton or gauze

7. Sub-tail