This document summarizes preclinical screening models of chronic obstructive pulmonary disease (COPD). It describes exposing mice to cigarette smoke over both acute (6 weeks) and chronic (5 days, 3 cigarettes/day) periods to model the effects of cigarette smoke, the main cause of COPD. For the acute study, mice are divided into groups exposed to air, smoke, or smoke with low- or high-dose test drugs. For the chronic study, groups are exposed to air, drugs and air, smoke, or smoke with low- or high-dose drugs. After exposure, the mice are sacrificed and their lungs examined to measure emphysema and macrophage volume as indicators of COPD pathology.

1. Submitted by,
Prajitha.P
PRECLINICAL SCREENING
MODELS OF COPD

2. COPD
 It is a severe respiratory condition that is
increasing prevalence world wide
 This disease is characterized by air flow limitation
that is not filly reversible
 The air flow limitation is usually progressive &
associated with abnormal inflammatory response
of the noxious particles and gases.

3. 3 condition comprises COPD
1. Mucus hypersecretion
2. Emphysema
3. Bronchiolitis
cigaratte smoke is the main cause

4. Chronic cigarette smoke exposure mice
6 week old male mice are selected
ACUTE STUDY
Exposed to smoke of 5
ciggarats for 20 mints
3gp of having 40 mice
are selected
CHRONIC STUDY
Exposed to 5 days with
3cig/day
5gps having 40 mice

5.  In acute study:
 Each 40 animal gp are sub divided & each
have 10 each
1.No tx/ air exposed
2.No tx/smoke exposed
3.Low dose test /smoke exposed
4.High dose test/smoke exposed

6.  In chronic study
1.No tx/air exposed
2.Drud tx/air exposed
3.No tx/smoke exposed
4.Low dose drug/smoke exposed
5.High dose drug/smoke exposed

7.  After 7 mints animal are sacrificed , lung fixed intra
tracheally with formalin soln. at a pressure of 20cm in
water
 Lung vol. measured by water displacement method
 Emphysema: mean linear intersept and internal surface
area
 Vol. of macrophage calculated by point count method

8. Thank you