This document summarizes information about platelet collection and transfusion. It discusses that platelets have a 5 day shelf life at room temperature and lose function when refrigerated. Plateletpheresis (single donor platelets) allows collection of therapeutic doses from one donor in 1.5-2 hours using specialized equipment, with lower risks than pooled platelet concentrates. Risks of platelet transfusion include bacterial and viral contamination. Single donor platelets have advantages over pooled platelets like lower reaction rates and one donor exposure. The document also outlines the Haemonetics commitment to continuous quality improvements and their blood processing technologies.

1. Haemonetics Commitment
Continuous improvement :
quality of final product, donor comfort and time saving
The partner you have chosen to develop blood component
collection programs in your country
Entirely dedicated to automated blood processing
technology

2. PLATELETS

3. Pool Platelets
Life span 5 days
Stored at room temperature
Lose function when refrigerated or transfused
into cold patient.
One platelet concentrate : 0.55 x 10e 11
suspended in 50 ml plasma.
pH > 6.5 after 72 hours storage at 22°C

4. 2 to 5 x 10e11 platelet yield
30% drop in donor platelet count replaced
in 48 hours
Low white cell contamination
Minimal donor red cell loss
Fewer donor reactions than whole blood
donations.
Plateletpheresis (Single Donor Platelet )

5. Pheresis Platelets
Storage: at 20o
C to 24o
C under
constant agitation. Maximum storage
time = 5 days
Therapeutic dose: between 2.0 – 5.0 x 1011
Platelets = 4-8 whole blood collections.

6. Pheresis Donation Vs Whole Blood Donation
Whole Blood Donation
No specialized equipment
10 mins
More uniform work flow
Mobile collection
Walk-ins
Autonomous operation
Pheresis Donation
Blood Cell Separator
Ave 1.5 to 2 hours
No shows, rejections,
deferrals very costly
Hospital/blood center
By appointment
Lab & medical access

7. Multi-Component System (MCS)
Single
Donor
1 blood test
Red cells and plasma
Platelets and plasma
Platelets
Plasma
Red cells
Automated solution addition
Volume compensation

8. Risk of blood transfusion
Viral infection Frequency per
million units
No. of deaths per
millions u.
Hepatitis A 1 0
Hepatitis B 7-32 0-0.14
Hepatitis C 4-36 0.5-17
HIV 0.4-5 0.5-5
HTLV (I/II) 0.5-4 0
Parvovirus B19 100 0

9. Risk of blood transfusion
Other Risk Factor Frequency per million
units
No. of deaths per
millions u.
Bacterial
contamination (Rbc)
2 0.1-0.25
Bacterial contam.
(Platelet)
83 21
Acute hemolytic
reactions
1-4 0.67
Delayed hemolytic
reactions
1000 0.4
Trali 200 0.2
Source: Goodnough et al, N Engl J Med 1999;340:438-447

10. Risks of Platelet Transfusion
Contamination of Platelets
The risk of platelet sepsis is greater with a
transfusion of pooled platelet
concentrates from multiple donors than
from a single donor.
Review Articles
The New England Journal of Medicine (Feb 11, 1999)

11. Risks of Platelet Transfusion
Platelet transfusion can transmit viral or bacterial
disease.
Contaminating red cells in the product may transmit
malaria.
Graft-versus-host disease (preventable by irradiation)
Cytomegalovirus (preventable by serological
screening)
Alloimmunization caused by contaminating white
cells.

12. Risk of Platelet transfusion
Leukocyte removed could possibly prevent all these
problems, but this is not established.
Febrile reactions are common and are reduced but
not totally eliminated by Leukocyte removal
Platelet themselves may cause fever.

13. One donor exposure only
For special feature collections (HLA-
matched, CMV-negative)
Lower reaction rate
Lower risk of alloimmunization and
transmission of viruses
Increased donor productivity
Advantages of Single Donor Platelets Over Pooled
Platelet Concentrate Transfusions

14. One Pretransfusion Test
Lower Cost than Manual Collection
Prompt Transfusion Possible
Less Unit Handling
Compliance with Quality System Standards
Advantages of Single Donor Platelets Over Pooled
Platelet Concentrate Transfusions

15. The aggressive treatment of hematological
malignancies created an increased demand
for platelets, and providing an adequate
supply of platelet concentrates became an
important responsibility for blood banks,
especially those serving hematology and
oncology depts.
Increased Demand For Platelets

16. Continuous Improvements
 Introduce MCS+ and automated in-line filtration LDP
Protocol in 1996
Pre-storage leukodepleted platelet concentrates
 Introduce Time-Saving LDP Protocol in 1999
Single dose of platelets in one hour
Double dose of platelets in 90 min. (High Precount Donor)
 Introduce Time-Saving improvements in 2000
Improve donor comfort (citrate control) while improving
performances
 Improve leukoreduction of double doses

17. Donor platelet count >280 x 10e3
Use the Haemo Calculator menu to set the Target
Yield as end of the procedure criteria.
Mixed the platelet concentrate thoroughly prior to the
splitting.
No more than 3.5 x 10e11 platelet is stored in a CLX
bag for up to 5 days.
No more than 5.0 x 10e11 platelet is stored in a CPP
bag for up to 5 days.
High-dose Collection

19. MCS+ Apheresis Equipment
MCS® +
Apheresis
#994E, #994EF
Plasma exchange
TPE: 980,981
Peripheral blood stem
cells for bone marrow
transplant & White cell
therapy
PBSC: 971E
Therapeutic red cell
TAE: 944
All blood bank
applications in one
mobile device

20. Single-Needle Systems
MCS® +
Filtration
Haemonet
HaemoCalc
PCS® +
Automated
surge
Small & easy to use
Multi-Component
Three pumps
Mobile
MCS® 3p