- This study describes the treatment of 5 critically ill COVID-19 patients with convalescent plasma.
- All 5 patients saw reductions in viral load and improvements in clinical symptoms and indexes after receiving 2 consecutive plasma transfusions.
- 3 patients were able to be weaned from mechanical ventilation after treatment.
- The study provides initial evidence that convalescent plasma may help reduce the severity of COVID-19 in critically ill patients, though more research is needed.
Treatment of covid 19 with failure of plasma therapy and their side effects Manali Chavan
The covid-19 pandemic caused by SARs-CoV-2 virus originated in China in December 2019, has now become a major concern all over the world. In battle against covid-19, convalescent plasma obtained from recently recovered cases of covid-19 is gaining attention as one of the treatment option.
Brief presentation about COVID19 diagnosis ,management and discharge criteria from isolation. Short Discussion about guideline given by Nepal medical council and TUTH for management.
Treatment of covid 19 with failure of plasma therapy and their side effects Manali Chavan
The covid-19 pandemic caused by SARs-CoV-2 virus originated in China in December 2019, has now become a major concern all over the world. In battle against covid-19, convalescent plasma obtained from recently recovered cases of covid-19 is gaining attention as one of the treatment option.
Brief presentation about COVID19 diagnosis ,management and discharge criteria from isolation. Short Discussion about guideline given by Nepal medical council and TUTH for management.
After the intravenous transplantation of MSCs, a significant population of cells accumulates in the lung, which they alongside immunomodulatory effect could protect alveolar epithelial cells, reclaim the pulmonary microenvironment, prevent pulmonary fibrosis, and cure lung dysfunction. The fact that the transplantation of MSCs improved the outcome of COVID-2019 patients may be due to regulating inflammatory response and promoting tissue repair and regeneration. This is a preliminary report of our study in Iran.
Antiretroviral Resistance in HIV-1 Patients at a Tertiary Medical Institute in Saudi Arabia: a Retrospective Study and Analysis.
Journal Club,
Virology Rotation , 1/5/2019
After the intravenous transplantation of MSCs, a significant population of cells accumulates in the lung, which they alongside immunomodulatory effect could protect alveolar epithelial cells, reclaim the pulmonary microenvironment, prevent pulmonary fibrosis, and cure lung dysfunction. The fact that the transplantation of MSCs improved the outcome of COVID-2019 patients may be due to regulating inflammatory response and promoting tissue repair and regeneration. This is a preliminary report of our study in Iran.
Antiretroviral Resistance in HIV-1 Patients at a Tertiary Medical Institute in Saudi Arabia: a Retrospective Study and Analysis.
Journal Club,
Virology Rotation , 1/5/2019
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Couples presenting to the infertility clinic- Do they really have infertility...
plasma therapy in covid.pptx
1.
2. Treatment of 5 Critically Ill Patients
With COVID-19 With Convalescent
Plasma
3. Introduction
• The epidemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
originating in Wuhan, China,has rapidly spread worldwide
• As of March, 2020, cases were reported in approximately 195 countries
• No specific therapeutic agents or vaccines for COVID-19 are available
• The use of convalescent plasma was recommended as an empirical treatment
during outbreaks of Ebolavirus in 2014
• A protocol for treatment of Middle East respiratory syndrome coronavirus with
convalescent plasma was established in 2015
4. Introduction
• In a study involving patients with pandemic influenza A(H1N1) 2009 virus infection,
treatment of severe infection with convalescent plasma (n=20patients) was associated
with reduced respiratory tract viral load, serum cytokine response, and mortality
• The purpose of this study was to describe the initial clinical experience with convalescent
plasma transfusion administered to critically ill patients with COVID-19.
5. Methodology
• This study was conducted at the infectious disease department,
Shenzhen Third People's Hospital, Shenzhen, China,
• From January 20,2020 to March 25,2020 and the final date of
follow-up was March 25, 2020.
6. Patient – inclusion criteria
• Patients with laboratory confirmed COVID-19, diagnosed using quantitative
reverse transcriptase–polymerase chain reaction (qRT-PCR) were eligible to
receive convalescent plasma treatment if they fulfilled the following criteria –
(1) severe pneumonia with rapid progression and continuously high viral load
despite antiviral treatment
(2) PAO2/FIO2 of <300 (PAO2 measured in mm Hg and FIO2 measured as fraction
of inspired oxygen)
(3) currently or had been supported with mechanical ventilation
7. Patient details
• The serum of each recipient was obtained
• ELISA and neutralizing antibody titers were tested one day prior to the convalescent
plasma transfusion
• Each received 2 consecutive transfusions of 200 to 250 mL of ABO-compatible
convalescent plasma (400 mL of convalescent plasma in total )on the same day it was
obtained from donor
• They received antiviral agents continuously until the SARS-CoV-2 viral loads became
negative
8. Disease Severity Classification
Patients with laboratory-confirmed COVID-19 infection who had any of the following were
considered in critical condition
• (1) respiratory failure requiring mechanical ventilation
• (2) shock, identified by the use of vasopressor therapy and elevated lactate
levels(>2mmol/L)despite adequate fluid resuscitation
• (3) failure of other organs requiring admission to the intensive care unit(ICU).
9. Donors
• 5 donors of convalescent plasma were between 18 and 60 years
• The donors had recovered from SARSCoV-2 infection
• The donors should be asymptomatic for at least 10 days, with a serum SARS-CoV-2–
specific ELISA antibody titer higher than 1:1000 and a neutralizing antibody titer greater
than 40.
• Following donation, 400 mL of convalescent plasma was obtained from each donor by
apheresis
• Plasma was immediately transfused to the recipients on the same day it was obtained
10. Data
Clinical information for the 5 patients before and after convalescent plasma
transfusion was obtained from are view of the hospital computer medical system-
• Demographic data
• days of admission from symptom onset
• presenting symptoms
• Mechanical ventilation , antiviral therapies, and steroids
• body temperature, PAO2/FIO2 ,and Sequential Organ Failure Assessment(SOFA)
score
11. Data
• Lab data - WBC ,lymphocyte count, LFT , KFT , cycle threshold value (Ct),
CRP procalcitonin, and IL-6, and serum antibody titer (IgG ,IgM ,and
neutralizing antibodies);
• Chest imaging studies
• Information on complications ,such as acute respiratory distress syndrome
(ARDS), bacterial pneumonia, and multiple organ dysfunction syndrome
12. Rt PCR
• Nasopharyngeal specimens collected during hospitalization were sent to the
laboratory in a viral transport case.
• Each RT-PCR assay provided a Ct value, which is the number of cycles required
for the fluorescent signal to cross the threshold for a positive test: a higher Ct
value is correlated with a lower viral load
• The specimens were considered positive if the Ct value was 37 or lower and
negative if the results were undetermined.
• The specimen was considered positive if the repeated results were the same as the
initial result and between 37 and 40.
• The Ct values of the 5 recipients were obtained on day−1,3,7, 12 after the
transfusion
13. • Microtiter plates were coated over night at 4°C with 4μg/mL
recombinant SARS-CoV-2 RBD (receptor binding domain) proteins (50 μL
per well) expressed through 293-T cells
• The plates were washed 3 times with phosphate-buffered saline (PBS)
containing 0.1% vol/vol Tween-20(PBST)
• Blocked with blocking solution (PBS containing 2%wt/ vol nonfat dry milk
) for 2 hours at 37°C
• The serum samples were diluted to 200-fold into PBS as initial
concentration
• After 3 washes, 100 μL of horseradish peroxidase–conjugated goat anti–
human IgG & IgM added & incubated at 37°C for 60 minutes.
14. • After 5 washes, 100 μL of tetramethylbenzidine substrate (Sangon
Biotech) was added at room temperature in the dark
• The absorbance was measured at 450nm . All samples were run in
triplicate.
• The ELISA titers were determined by end point dilution
15. • Vero cells were seeded in a 96-well plate
• Serum samples from patients were incubated at 56°C for 30 minutes and then diluted 2-
fold
• Aliquots (40 μL) of diluted serum samples were added to 50 μL of cell culture medium
containing TCID50 of virus
• Virus antibody mix was then added to cells in 96-well plates
• Microscopic examination for cytopathic effect done after a 5-day incubation
• The highest dilution of serum that showed inhibition activity of SARS-CoV-2was
recorded as the neutralizing antibody titer
19. Temporal changes of Cycle Threshold value, SOFA score, in patients
receiving convalescent Plasma Transfusion
A- Change in cycle threshold (Ct) value in nasopharyngeal swabs of infected patients at day 0,day 3,day 7,andday
12after the plasma transfusion . A Ct value of 40 was defined as undetectable.
B- Change in Sequential Organ Failure Assessment (SOFA) score of the patients with convalescent plasma
treatment (range 0-24
20. Temporal changes of PaO2/FiO2 & body temperature , in patients receiving
convalescent Plasma Transfusion
21. b, c- ELISA end point dilution titers (IgG and IgM antibody).The expected titer of negative control from a healthy
person <200
D Neutralization end point dilution titers.The expected titer of negative control from a healthy personis10
22. Changes of Receptor Binding Domain–Specific IgG and IgM ELISA and Neutralizing Antibody Titers
Before and After Convalescent Plasma Transfusion in Patients
23. Results
• All 5 patients were receiving mechanical ventilation at the time of transfusion ,and
3 patients (patients3,4,and5) were weaned from mechanical ventilation
• Patient2 was receiving ECMO at the time of plasma treatment but did not require
ECMO on day 5 after transfusion
• Patients 3,4,and 5 were discharged from the hospital (length of stay: 53, 51, and
55 days, respectively
24. Discussion
• Plasma was obtained from the donors and transfused in the recipients on the same
day, which helps preserve the natural activity of the plasma.
• Viral load decreased and became undetectable so on after convalescent plasma
treatment .As determined by ELISA , all plasma from the donors had high virus-
specific IgG and IgM ELISA titers
• the neutralizing antibody titers, vital for the restriction of viral infection of the 5
recipients ,significantly increased after plasma transfusion.
25. • All patients received antiviral agents ,including interferon and
lopinavir/ritonavir ,during and following convalescent plasma
treatment, which also may have contributed to the viral clearance
observed.
26. Limitations
• First, this was a small case series that included no controls
• It is unclear if these patients would have improved without transfusion of
convalescent plasma, although the change in Ct and PAO2/ FIO2 represent
encouraging findings
• All patients were treated with multiple other agents (including antiviral
medications),and it is not possible to determine whether the improvement observed
could have been related to therapies other than convalescent plasma.
• Plasma transfusion was administered 10 to 22 days after admission ; whether a
different timing of administration would have been associated with different
outcomes cannot be determined
27.
28. • The epidemic spread rapidly worldwide within 3 mo and was
characterized as a pandemic by WHO on March 11, 2020
• Currently, there are no approved specific antiviral agents targeting
the novel virus, while some drugs are still under investigation
• Convalescent plasma (CP) therapy, a classic adaptive immunotherapy,
has been applied to the prevention and treatment of many infectious
diseases for more than one century
29. • A meta-analysis from 32 studies of SARS coronavirus and severe
influenza showed a statistically significant reduction in the pooled
odds of mortality following CP therapy, compared with placebo or no
therapy (odds ratio, 0.25; 95% CI- 0.14–0.45)
• Patients who have recovered from COVID-19 with a high neutralizing
antibody titer may be a valuable donor source of CP.
• the potential clinical benefit and risk of convalescent blood products
in COVID-19 remains uncertain.
30. • this pilot study was done in three participating hospitals to explore
the feasibility of CP treatment in 10 severe COVID-19 patients.
31. Neutralizing Activity of CP against SARS-CoV-2
• The neutralizing activity against SARS-CoV-2 was evaluated by
classical plaque reduction test using a recently isolated viral strain
• Among the first batch of CP samples from 40 recovered COVID-19
patients,39showedhighantibodytitersofatleast1:160,whereas only
one had an antibody titer of 1:32.
• This result laid the basis for pilot clinical trial using CP in severe
patients.
32. General Characteristics of Patients in the Trial
• From January 23, 2020, to February 19, 2020, 10 severe COVID-19 patients
(six males and four females) were enrolled and received CP transfusion
• The median time from onset of symptoms to hospital admission and CP
transfusion was 6 d (IQR, 2.5 d to 8.5 d) and 16.5 d (IQR, 11.0 d to 19.3 d),
respectively.
• The most common symptoms at disease onset were fever (7 / 10 s), cough
(8 ), and shortness of breath (8 ),
• Less common symptoms -sputum production (5), chest pain (2), diarrhea
(2), nausea and vomiting (2), headache (1), and sore throat (1).
33. Patient details
• 4 - had underlying chronic diseases- cardiovascular and/or cerebrovascular diseases and HTN
• 9 - received arbidol monotherapy or combination therapy with remdesivir, or ribavirin, or
peramivir, while one patient received ribavirin monotherapy
• 6 patients received intravenous (i.v.) methylprednisolone (20 mg every 24 h).
• All patients – in CT , bilateral ground-glass opacity and/or pulmonary parenchymal
consolidation with predominantly subpleural and bronchovascular bundles distribution in the
lungs
• Seven patients had multiple lobe involvement, and four patients had interlobular septal
thickening.
34.
35.
36. Effects of CP Transfusion- Improvement of
clinical symptoms
• All symptoms in the 10 patients, especially fever, cough, shortness of
breath, and chest pain, disappeared or largely improved within 1 d to
3 d upon CP transfusion.
• Prior to CP treatment - A fter CP
o 3 patients mechanical ventilation 2 weaned
o 3 received high-flow nasal cannula O2 1 discontinued
37.
38. Reduction of pulmonary lesions on chest CT
examinations
• all patients showed different degrees of absorption of pulmonary
lesions after CP transfusion
39. Fig. 1. Chest CTs of two patients. (A) Chest CT of patient 9 obtained on February 9 (7 dpoi) before CP
transfusion (10 dpoi) showed ground-glass opacity with uneven density involving the multilobal segments
of both lungs. The heart shadow outline was not clear. The lesion was close to the pleura. (B) CT Image of
patient 9taken on February 15 (13 dpoi) showed the absorption of bilateral ground-glass opacity after CP
transfusion
40. Chest CT of patient 10 was obtained on February 8 (19 dpoi) before CP transfusion (20 dpoi). The brightness of both lungs was
diffusely decreased, and multiple shadows of high density in both lungs were observed. (D) Chest CT of patient 10 on February 18
(29 dpoi) showed those lesions improved after CP transfusion.
43. Increase of neutralizing antibody titers and disappearance of
SARS-CoV-2 RNA
• The neutralizing antibody titers of five patients increased and four patients
remained at the same level after CP transfusion
• SARS-CoV-2 RNA was decreased to an undetectable level in three patients on day
2, three patients on day 3, and one patient on day 6 after CP therapy.
44.
45. Outcome of patients treated with CP as compared to a recent
historic control group
• A historic control group was formed by random selection of 10 patients from the cohort
treated in the same hospitals and matched by age, gender, and severity of the diseases to
the 10 cases in our trial
• 3 cases discharged while 7 cases in much improved status and ready for discharge in CP
group
• 3 deaths, 6 cases in stabilized status, and one case in improvement in the control group (P
< 0.001);