This document discusses several applications of plant tissue culture, including clonal propagation to produce genetically identical copies of plants, synthetic seed production, somatic hybridization to create hybrids between sexually incompatible plants, and cryopreservation for long-term germplasm storage. Clonal propagation is used in horticulture and forestry to multiply desirable traits from a single donor plant. Synthetic seeds encapsulate somatic embryos to allow for disease-free propagation of asexually reproducing plants. Somatic hybridization uses isolated protoplasts to create hybrids and introduce traits from wild plants to crops. Cryopreservation stores plant tissues and organs at ultra-low temperatures in liquid nitrogen for long-term conservation of plant germplasm.