Photosynthesis
Agenda
1.       Curriculum Expectations
2.       Plant structure
     •   Leaves, Stomata, Chloroplasts
3.       Pigments & Absorbance spectrum
4.       Light-dependant reactions
5.       Calvin Cycle
6.       Photorespiration
SPECIFIC CURRICULUM EXPECTATIONS
for Photosynthesis
   C2.1 use appropriate terminology related to metabolism, including, but not limited
    to: energy carriers, glycolysis, Krebs cycle, electron transport chain, ATP synthase,
    oxidative phosphorylation, chemiosmosis, proton pump, photolysis, Calvin cycle,
    light and dark reactions, and cyclic and noncyclic phosphorylation [C]

   C2.3 conduct a laboratory investigation of the process of photosynthesis to identify
    the products of the process, interpret the qualitative observations, and display them
    in an appropriate format [PR, AI, C]

   C3.2 explain the chemical changes and energy conversions associated with the
    process of photosynthesis (e.g., carbon dioxide and water react with sunlight to
    produce oxygen and glucose)

   C3.3 use the laws of thermodynamics to explain energy transfer in the cell during
    the processes of cellular respiration and photosynthesis
   C3.4 describe, compare, and illustrate (e.g., using flow charts) the matter and
    energy transformations that occur during the processes of cellular respiration
    (aerobic and anaerobic) and photosynthesis, including the roles of oxygen and
    organelles such as mitochondria and chloroplasts
What is Photosynthesis?
  Primary goal:
  Convert electromagnetic energy into
  useable chemical energy
Leaves
Stomata (Stoma)
   Allows passage of O2, CO2
    and H2O
   Opening regulated by a
    pair of photosynthetic
    guard cells
   Size of guard cells changes
    based on osmosis of water
    in or out of cell
   Osmosis direction follows
    the diffusion of K+ ions
    across the membrane
Mesophyll
    Tissue between the epidermal layers
    Lots of chloroplasts ... lots of
     photosynthesis
    Surrounded by vascular bundles
    2 distinct layers
    1)   Palisade
    2)   Spongy
Vascular Bundles
 Transport water and dissolved materials to
  the mesophyll
 Transports food (from photosynthesis) from
  the mesophyll to other parts of the plant
Chloroplast      In plants only
                 Contains the pigment
                  chlorophyll (cause of
                  green colour), allowing
                  it to undergo
                  photosynthesis
                 Two membranes
                 Contains its own DNA
Chloroplasts
Chlorophyll Structure
                             Porphyrin Ring
                              Contains e- that
                               absorb light energy
         Chlorophyll a has     and begin
         a methane
                               photosynthesis
         Chlorophyll b has
         an aldehyde         Hydrocarbon Tail
                              Anchors molecule
                               to membrane
Why are plants green?
Electromagnetic Spectrum
History:
George Englemann’s experiment
Absorption Spectrum of
Chlorophyll
Absorption Spectrum of all pigments
Chlorophyll - fluorescence
Chlorophyll a - Electron Donor
Overview of Photosynthesis
   Carbon Dioxide + Water + Light  Glucose + Oxygen
         CO2      +   H2O + Light  C6H12O6      + O2

2      stages:
       Light Dependent Rxn (thylakoid)
       Light Independent Rxn aka Calvin Cycle (stroma)

         6CO2 + 6 H2O + Light  C6H12O6         + 6O2
Change in energy levels
Overview of photosynthesis
Light-dependent Reaction
Light-dependent Reaction
Calvin Cycle
 Outsideof the Thylakoid – in the stroma
 Misconception: Not “DARK REACTION”
 3 phases:
     Phase 1 – Carbon Fixation
     Phase 2 – Reduction Reactions
     Phase 3 – Regeneration of RuBP
Calvin Cycle
Calvin Cycle
Lastly, Photorespiration
•   Consequences of wasteful oxygenation of
    Rubisco – addition of oxygen instead of
    CO2 (about 25% of plants)
•   C3 plants
•   C4 and CAM plants

VIDEO

Photosynthesis

  • 1.
  • 2.
    Agenda 1. Curriculum Expectations 2. Plant structure • Leaves, Stomata, Chloroplasts 3. Pigments & Absorbance spectrum 4. Light-dependant reactions 5. Calvin Cycle 6. Photorespiration
  • 3.
    SPECIFIC CURRICULUM EXPECTATIONS forPhotosynthesis  C2.1 use appropriate terminology related to metabolism, including, but not limited to: energy carriers, glycolysis, Krebs cycle, electron transport chain, ATP synthase, oxidative phosphorylation, chemiosmosis, proton pump, photolysis, Calvin cycle, light and dark reactions, and cyclic and noncyclic phosphorylation [C]  C2.3 conduct a laboratory investigation of the process of photosynthesis to identify the products of the process, interpret the qualitative observations, and display them in an appropriate format [PR, AI, C]  C3.2 explain the chemical changes and energy conversions associated with the process of photosynthesis (e.g., carbon dioxide and water react with sunlight to produce oxygen and glucose)  C3.3 use the laws of thermodynamics to explain energy transfer in the cell during the processes of cellular respiration and photosynthesis  C3.4 describe, compare, and illustrate (e.g., using flow charts) the matter and energy transformations that occur during the processes of cellular respiration (aerobic and anaerobic) and photosynthesis, including the roles of oxygen and organelles such as mitochondria and chloroplasts
  • 4.
    What is Photosynthesis? Primary goal: Convert electromagnetic energy into useable chemical energy
  • 5.
  • 6.
    Stomata (Stoma)  Allows passage of O2, CO2 and H2O  Opening regulated by a pair of photosynthetic guard cells  Size of guard cells changes based on osmosis of water in or out of cell  Osmosis direction follows the diffusion of K+ ions across the membrane
  • 7.
    Mesophyll  Tissue between the epidermal layers  Lots of chloroplasts ... lots of photosynthesis  Surrounded by vascular bundles  2 distinct layers 1) Palisade 2) Spongy
  • 8.
    Vascular Bundles  Transportwater and dissolved materials to the mesophyll  Transports food (from photosynthesis) from the mesophyll to other parts of the plant
  • 9.
    Chloroplast  In plants only  Contains the pigment chlorophyll (cause of green colour), allowing it to undergo photosynthesis  Two membranes  Contains its own DNA
  • 10.
  • 11.
    Chlorophyll Structure Porphyrin Ring  Contains e- that absorb light energy Chlorophyll a has and begin a methane photosynthesis Chlorophyll b has an aldehyde Hydrocarbon Tail  Anchors molecule to membrane
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
    Chlorophyll a -Electron Donor
  • 19.
    Overview of Photosynthesis  Carbon Dioxide + Water + Light  Glucose + Oxygen CO2 + H2O + Light  C6H12O6 + O2 2 stages:  Light Dependent Rxn (thylakoid)  Light Independent Rxn aka Calvin Cycle (stroma) 6CO2 + 6 H2O + Light  C6H12O6 + 6O2
  • 20.
  • 21.
  • 22.
  • 23.
  • 24.
    Calvin Cycle  Outsideofthe Thylakoid – in the stroma  Misconception: Not “DARK REACTION”  3 phases:  Phase 1 – Carbon Fixation  Phase 2 – Reduction Reactions  Phase 3 – Regeneration of RuBP
  • 25.
  • 26.
  • 28.
    Lastly, Photorespiration • Consequences of wasteful oxygenation of Rubisco – addition of oxygen instead of CO2 (about 25% of plants) • C3 plants • C4 and CAM plants VIDEO

Editor's Notes

  • #6 Cuticle – protection, prevents water loss etc. Epidermis is not photosynthetic
  • #10 Bacteria or protists that photosynthesize do not have a membrane-bound organelle chloroplast – they have pigments that function similar but do not have a specific organelle like plants.
  • #11 This is where all photosynthesis reactions take place.Why are plants green?
  • #13 (a) The greenish solution appeared to have a reddish tinge. (b) The green leaf appeared green from all angles. (c) The chlorophyll solution changed to a reddish colour when bright white light was shone on it. The red light was emitted by the excited electrons of chlorophyll molecules that had absorbed a photon of light. An electron in the chlorophyll molecule becomes excited when it absorbs electromagnetic radiation, and, since there is no compound available to accept the excited electron in a solution containing only chlorophyll molecules, the excited electron returns to its lowest energy level, reemitting the radiation it originally absorbed. This reemission of absorbed radiation is termed fluorescence. (d) The leaf did not appear reddish because photosynthesis will capture the energy and the carotenoids present in the leaf dissipate the excess absorbed energy as heat.