1) The study evaluated the biocompatibility and osseointegration of titanium dioxide nanocrystals synthesized using a wet precipitation method as a potential alloplastic bone graft material. 2) Histological analysis after 4 and 12 weeks of implantation in guinea pig jaws found signs of inflammation but also osteoid formation, osteocyte presence, and direct bone deposition on the titanium dioxide crystals. 3) The results demonstrate the biocompatibility and osseointegration potential of the titanium dioxide nanocrystal material at the nanoscale, suggesting it may be suitable for use as an alloplastic bone graft.

1. 65
BONE ACCESSION TO TIO2 NANOCRYSTALS:
EVIDENCE OF BIOCOMPATIBILITY AND OSSEOUS INTEGRATION
RJ Almeida, DSS Meireles*, ACB Filho, MC Pereira, AC Lima, ACA Silva, AM Loyola, NO Dantas, CJA Sousa.
drronaldoalmeida@hotmail.com
It has been estimated that a 500 thousand bone craft procedures is made each year around the world. Syntetase titanium dioxide (TiO2) nanocrystals on high degree of pureness; Evaluate its biocompatibility following
However little success is being reached in procedures using aloplastic materials, essentially because its 1 2
parameters defined and accepted for the Dental International Federation ( FDI) and American Dental Association (ADA):
osseointegrate incapacity.
Evidence its nanotopograph in the interface of the new bone through Atomic Microscopy Force (AMF)
Thus, through the methodology used was possible to evidence the osseointegration phenomenal and biocompatibility of an
aloplasthic material containing nanocrystals of titanium dioxide. We can also conclude that there was direct bone deposition on
the oxide, what can be called osteointegration. The material was gotten through the synthesis of a nano powder using the
3
humid one way precipitation, Pechini method on ambient temperature (Fig. 1). After temperature treatment was structurally
characterized by X-ray Diffraction (XRD) (Fig. 2a), Confirming titanium dioxide synthesis on Rutilo and Anatasio phases, with
tetragonal crystalline symmetry and medium size of 21 nm (Fig. 3). We characterize using de Raman micro spectroscopy too.
(Fig. 2b) The vibration asset ways typical on TiO2 nanocrystals on Rutilo e Anatase phases, confirming the pureness on the
synthesis through the absence of others nanostructures identified by Raman specter (Fig. 4). After that, the biocompatibility
test was made using intra bone implantation of the TiO2 powder inside a teflon cup in guinea pigs jaw. (Fig. 5) Previously Figura 1
approved by the CEUA/UFU 101/10. Ten guinea pigs were used on the experimental periods: 4 and 12 weeks. Each animal
received a Teflon cup containing TiO2 nanocrystals. From each implant was made 24 blades with 144 cuts colored by
hematoxilin-eosine method and analyzed by two independent observers by means of inflammatory intensity response scores, Figura 2 Figura 3 Figura 4 Figura 5
considering all Teflon cup board. The analysis was criteriously interpreted following the FDI normative as acceptable or not
acceptable to be related to bone.
Thus, through the methodology used it was possible to demonstrate the
Histologycal Parameters Number of Implants TiO2 NanoCristals Control phenomenon of osseointegration and biocompatibility,at the nanoscale, of
Material Experimental Periods (weeks) Number of Implants
4 weeks 12 weeks 4 weeks 12 weeks
30 10 Neutrophils + + + + na alloplastic material containing titanium dioxide nanocrystals. We also
Macrophages + + + +
TiO2 90 10 Linphocytes + + + +
Plasma cells + + + +
conclude that there is direct bone deposition on the oxide, which could be
30 10 Giant cells ++ ++ + +
Control 90 10 Scatterd material ++ ++ + + called oxideintegration.
Capsule + + + +
Remodeling bone tissue +++ +++ + +
Necrotic bone tissue + + + +
Reabsorption + + + +
Inflammatory reaction + + + +
--------------------------------------------------------------------------------------------------
Absent ( +) ; Moderated (++); Severe (+++)
The Foundation for Research Support of the Minas Gerais State- FAPEMIG-
Table 01 = Table 02 = for financial support through the process APQ-No. 02561-10
Experimental time and implant number
Fig 6: (a) General vision, (b) giant cells delicate layer, (c) osteóide material, (d) Osteocite presence and TiO2 attached to bone. Evaluation of inflammatory reaction after 04 weeks and 12 weeks.
1- Technical Report no 9 F.D.I. Langeland,K; Cotton,W.R. Recommended
standard practices for biological evaluation of dental materials. International
Dentistry Journal, 1980; 30(2): 140-88.
2- American Dental Association-American National Standards Institute.
Document No 41 for recommend standard practices for biologic evaluation of
dental materials. Council of Dental Materials and Devices, 1982.
3- Pecchini,M.P; Adams,N. Method of preparing lead and alkaline earth
titanates and niobates and coating method using tha same to form a
Figure 7: (a) General vision, (b) appalling of the material, (c) material on gaps and bone and lamellar bone formation, (d) direct bone deposition. capacitor. United States Patent Office, 1967.