This study investigated the effects of various artificial sweeteners on chick embryo development. Chick embryos were exposed to different concentrations of aspartame, saccharin, sucralose, and sucrose through in ovo injection. After 7 days of incubation, the embryos were examined. Results showed significant differences in body weight, length, and cell size between treatment groups. Aspartame exposure resulted in irregular cell shapes. Sucralose exposure decreased body weight and length the most. Saccharin increased body weight. Sucrose increased cell size. Overall, the study found artificial sweeteners can have teratogenic effects on developing chick embryos.