An excellent ppt on basics of bone marrow morphology and examination which i came accross on the internet.. Not my creation.. Full credit to the author..
An excellent ppt on basics of bone marrow morphology and examination which i came accross on the internet.. Not my creation.. Full credit to the author..
There are several important changes in the WHO 5th edition hemato-lymphoid with a paradigm shift towards genetic diagnosis along with morphological aspects. Precursor lesions of Clonal hematopoiesis, CHIP and CCUS are formally included, Changes include those in AML, MPN, JMML is now a part of MPN, MDS-MPN, ALAL etc.
Plasma cell disorders is a difficult topic where most residents and students confuse with regarding to differentiating between various types of para-proteinemias or plasma cell dyscrasias. This simple presentation will highlight the key points in differentiating, diagnosing these orders. Initial management principles are discussed as well.
For undergradutes
Revise structure of lymph node and spleen
Classify non-neoplastic lesions
Various histological patterns
Etiologies of each lesion / pattern
processing of bone marrow trephine biopsykanwalpreet15
there is no standard method for processing of bone marrow trephine biopsies. there are various fixatives and decalcifying agents . depending upon need of IHC and cytogenetics, we can decide
There are several important changes in the WHO 5th edition hemato-lymphoid with a paradigm shift towards genetic diagnosis along with morphological aspects. Precursor lesions of Clonal hematopoiesis, CHIP and CCUS are formally included, Changes include those in AML, MPN, JMML is now a part of MPN, MDS-MPN, ALAL etc.
Plasma cell disorders is a difficult topic where most residents and students confuse with regarding to differentiating between various types of para-proteinemias or plasma cell dyscrasias. This simple presentation will highlight the key points in differentiating, diagnosing these orders. Initial management principles are discussed as well.
For undergradutes
Revise structure of lymph node and spleen
Classify non-neoplastic lesions
Various histological patterns
Etiologies of each lesion / pattern
processing of bone marrow trephine biopsykanwalpreet15
there is no standard method for processing of bone marrow trephine biopsies. there are various fixatives and decalcifying agents . depending upon need of IHC and cytogenetics, we can decide
complete information about the cancer condition that is leukemia - introduction, definition, etiology and causes, pathophysiology ,types, clinical manifestations, diagnosis, nursing management, medical management, nursing research .
What is Lymphoma?
Malignant lymphoma is a term given to tumors of the lymphoid system and specifically of lymphocytes and their precursor cells
i.e.
Cancer of the lymphatic system.
Many lymphomas are known to be due to specific genetic mutations.
Ang pagbubukas ng Suez Canal (THE OPENING OF SUEZ CANAL)hm alumia
ANG PAG USBONG NG NASYONALISMONG PILIPINO:
Ang pagbubukas ng Suez Canal
(THE OPENING OF SUEZ CANAL)
The Suez Canal is an artificial sea-level waterway in Egypt, connecting the Mediterranean Sea to the Red Sea through the Isthmus of Suez, and separates the African continent from Asia. After 10 years of construction, it was officially opened on November 17, 1869. The canal allows ships to travel between Europe and South Asia without navigating around Africa, thereby reducing the sea voyage distance by about 7,000 kilometres (4,300 mi). It extends 193.30 km (120.11 mi) from the northern terminus of Port Said to the southern terminus of Port Tewfik at the city of Suez. In 2012, 17,225 vessels traversed the canal (47 per day).
The canal is a single-lane waterway with passing locations in the Ballah Bypass and the Great Bitter Lake. It contains no locks system, with seawater flowing freely through it. In general, the canal north of the Bitter Lakes flows north in winter and south in summer. South of the lakes, the current changes with the tide at Suez.
The canal is owned and maintained by the Suez Canal Authority[4] (SCA) of Egypt. Under the Convention of Constantinople, it may be used "in time of war as in time of peace, by every vessel of commerce or of war, without distinction of flag."
In August 2014, construction was launched to expand and widen the Ballah Bypass for 35 km (22 mi) to speed the canal's transit time. The expansion is expected to double the capacity of the Suez Canal from 49 to 97 ships a day. At a cost of $8.4 billion, this project was funded with interest-bearing investment certificates issued exclusively to Egyptian entities and individuals. The "New Suez Canal", as the expansion was dubbed, was opened with great fanfare in a ceremony on 6 August 2015.
https://en.wikipedia.org/wiki/Suez_Canal
Presidents of the Philippines (Era & Constitutions) Summaryhm alumia
Presidents of the Philippines
Philippine Presidents
First Republic of the Philippines (Malolos Republic)
Commonwealth of the Philippines
Second Republic of the Philippines
Third Republic of the Philippines
Fourth Republic of the Philippines
Fifth Republic of the Philippines
Gymnastics
- History and Nature of Gymnatics
- Equipments for Gymnastics
- Costumes for Gymnastics
- Basic Skills in Gymnastics
- Stretching and Conditioning in Gymnastics
- Safety Rules in Gymnastics
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
2. History
1907 – Turk
First describe lymphocytes in a patient whose disease had
been diagnosed as acute leukemia and later recovered.
He describe cells as having an immature nucleus and
basophilic cytoplasm similar to that of a plasma cell.
1923 – Downey and McKinlay
“Acute Lymphadenosis Compared with Acute Lymphatic
Leukemia”.
1967 – Wood and Frenkel
Important morphologic and biochemical characteristics of
variant lymphocytes in various clinical entities.
3. Morphology of Variant or Reactive
Lymphocytes
• The most common important
feature of variant lymphocyte
morphology is the recognition
of its benign nature.
• These lymphocytes are normal
cells that have been altered as
a result of a normal response
to stimulus.
• Downey and McKinlay – classic
description of the reactive
lymphocyte.
4. Morphology of Variant or Reactive
Lymphocytes
• Three distict types:
o Type I
Plasmacytoid lymphocyte or Turks’s irritation cells.
o Type II
Infectious Mononucleosis (IM)
o Type III
Transformed lymphocytes or Reticular lymphocytes
5. Morphology of Variant or Reactive
Lymphocytes
Type I – “Plasmacytoid lymphocyte” or “Turks’s irritation cells”.
• Differentiated cells that are functionally immunocompetent
and probably of B-cell origin.
• Size: 9 to 20 um diameter
• Shape: Oval to Round
• Nucleus: Heavy strands or dense blocks of chromation
irregularly clumped with sharp, small, defined areas of
parachromatin; nuclear shape may be intented or oval. Nucleus
membrane is distinct.
• Cytoplasm: Basophilia varies but usually the cytoplasm is
moderately basophilic. It may be vacuolated, with darker areas of
basophilia at the periphery. It may also have a foamy appearance
and may contain azurophilic granules.
6. Morphology of Variant or Reactive
Lymphocytes
Type II – “Infectious Mononucleosis (IM)”
• They are predominant in Infectious Mononucleosis.
• Size: 15 to 25 um in diameter
• Shape: Irregular or Scalloped
• Nucleus: The chromatin strands are coarse but not as
condensed as those of type I. Rounded masses of chromatin are
interspersed throughout. Nuclear shape is round or oval and is
rarely lobulated. Nuclear banding frequently is seen in EDTA
specimens. Nucleoli usually are not visible.
• Cytoplasm: Abundant and often indented by surrounding
structures. Nuclear:cytoplasmic ration is 1:2 to 1:4. The cytoplasm
has few vacuoles and usually is pale, except for basophilia at the
periphery of the cytoplasm and radiating from the nucleus. This cell
often has been describe s resembling a friend egg or a flattered
skirt.
7. Morphology of Variant or Reactive
Lymphocytes
Type III – “Transformed lymphocytes” or “Reticular lymphocytes”
• Cells in intermediate stage of transformation, the process
through which the resting small lymphocyte undergoes blast
transformation and ultimately becomes a fully immunocompetent
T lymphocyte or plasma cell.
• Size: 12 to 35 um in diameter
• Shape: Round to irregular
• Nucleus Finely reticulated nuclear chromatin (immature).
Chromatin strands are finely dispersed with loose, indistinct
clumping and poorly defined parachromatin. Nucleoli are usually
highly visible and elongated or irregular in shape.
• Cytoplasm: Vacuolated with abundant basophilia and a clear
perinuclear area.
8. Lymphocyte Transformation
• The lymphocyte morphology describe reflects
the cumulative events following the antigenic
stimulation in which the stimulated
lymphocyte undergoes structural and
biochemical changes, transforming the small
lymphocytebto the blastlike-cell, the process
is called blastogenesis.
• Transformation can be produced in vitro by a
specific and non-specific antigens.
9. Lymphocyte Transformation
• Non-specific antigens used to stimulate
lymphocyte in vitro:
Phytohemagglutinin (PHA)
Pokeweed Mitogen (PWM)
Streptolysin S Staphylococcus Endotoxin (SLS)
Antilymphotcyte Globulin (ALG)
• Most commonly used are: PHA and PWM for
both T and B cell stimulation leading to mitosis.
Cultures in using PHA show nucleolar changes in 4
hours and RNA production within 8 hours; at the end
of 72 hours most cells are transformed and in mitosis.
10. Lymphocyte Transformation
• Transmission Electron Microscope
Nucleus becomes larger and clearer.
Cytoplasm contains enlarge Golgi Apparatus.
Ribosome increase in number.
Mitochondria increase in volume.
Endoplasmic Reticulum develops slightly.
Azurophilic granules increase in number.
Nucleoli become elongated and enlarged.
• Scanning Electron Microscope
Reveals a pronounce shape change from round to “hand
mirror” shape.
Uropod becomes very prominent.
11. Differentiation between Reactive and
Malignant Lymphocytes
• Both reactive and malignant lymphocytosis
may exhibit immature looking cells.
• The major morphologic differentiation lies in
the heterogeneity of the variant lymphocytes
(polymorphism).
• Malignancies are clonal, and all abnormal cells
appear very similar to the other one.
13. Absolute Lymphocytosis with Variant
Lymphocyte Morphology
• Infectious Mononucleosis
A clinical acute contagious viral disease that
affects primarily young adults and teenagers.
Self-limited and benign, but serious complications
can occur which occasionally may be fatal.
Characterized by variant (reactive) lymphocytes in
the peripheral blood and heterophil antibody-
positive serologic test.
14. Absolute Lymphocytosis with Variant
Lymphocyte Morphology
• History:
1885 – Filatov – idiopathiclymphadenopathy in children.
1889 – Pfeiffer – lymphatic reaction in children.
1920 – Sprunt and Evans – used the term “Infectious
Mononucleosis”.
1923 – Downey and McKinlay – morphology of the reactive
lymphocytes.
1932 – Paul and Bunnell – Serologic characterization.
1955 – Davidsohn and coworkers – refined serologic
characterization.
1964 – Epstein. Anchong and Barr – herpes-like virus (EBV-
Epstein Barr Virus)
15. Absolute Lymphocytosis with Variant
Lymphocyte Morphology
• Clinical Features:
Incubation period of IM is about 11 days.
Fever, pharyngitis, and cervical lymphadenopathy are
presenting symptoms in more than 80% of cases.
Splenomegaly is found in 50% of cases and hepatomegaly in
approximately 10% of patients.
Rash is present in 20% of cases.
Complications are pneumonitis, meningoencephalitis,
pericarditis, myocarditis, hepatitis, and laryngeal edema, all
of which are related to lymphocytic infiltrates.
Neurologic syndromes such as Bell’s palsy and Guillain-Barre
usually are reversible.
Clinical complications include hemorrhage due to
thrombocytopenia, airway obstruction due to enlargement
of pharyngeal lymphoid tissue, and splenic rupture is
splenomegaly is present.
16. Absolute Lymphocytosis with Variant
Lymphocyte Morphology
• Cytomegalovirus Infection
• Definition and Clinical Features:
It is a disease caused by cytomegalovirus that closely
resembles IM.
Most CMV infections appear to be subclinical.
Fever and Splenomegaly are common in middle-aged
adults.
Hematomegaly may be found in 50% of patients and a
rash may be present.
Illness, malaise, fever and chills are common,
symptoms may persist for longer period (3 weeks)
Incubation period is 35 to 40 days in adults, and 20 to
25 days for children.
17. Absolute Lymphocytosis with Variant
Lymphocyte Morphology
• Pathophysiology:
The virus is found in urine, oral and cervical secretions,
and semen, as well as in leukocytes.
Transmission in adults is primarily venereal.
More than half of adults possess antibodies to CMV.
19. Absolute Lymphocytosis with Normal
Lymphocyte Morphology
• Acute Infectious Lymphocytosis
• Usually found in children between ages of 1 to 10 years,
and occasionally up to 14 years of ages.
• It is contagious, benign, and self-limited.
• Causative agent maybe viral or non-viral.
• The incubation periods appears to be between 12 to 20
days.
• The disease lasts from 3 to 5 weeks and may last as long as
2 months.
• Clinical Features:
Asymptomatic – patients with infectious lymphocytosis
Symptoms accompany the disease are fever, upper respiratory
infection, diarrhea, and abdominal pain.
20. Absolute Lymphocytosis with Normal
Lymphocyte Morphology
• Bordetella Pertussis Infection
Infection in which 79 to 90% of leukocytes on the
peripheral blood film are normal looking lymphocytes.
The increase in small lymphocytes maybe due to
redistribution from tissue pools to circulating pools
caused by a lymphocyte-promoting factor (LPF).
The leukocytosis and lymphocytosis are pronounced
than in any other febrile illness except IM.
21. Absolute Lymphocytosis with Normal
Lymphocyte Morphology
• Lymphocytic Leukemoid Reaction
Any condition in which the lymphocytic
leukocytosis is so marked that it gives impression
of possible leukemia qualifies as a lymphocytic
leukemoid reaction.
Infectious Mononucleosis in children may present
with leukocyte counts in excess of 50x109/L, which
may lead to an impression of acute lymphocytic
leukemia.
23. Relative Lymphocytosis with Variant
Lymphocyte Morphology
• Toxoplasmosis
Toxoplasma (Toxoplsma gondii) infection is similar in
clinical presentation to IM, causing fever and enlarged
lymph nodes.
The result of the heterophil antibody test is negative.
Laboratory features are benign, with normal
hematologic parameters, the exception being a
relative increase in lymphocytes and the presence of
reactive lymphocytes.
Current test for confirmatory are indirect fluorescent
antibody and indirect hemagglutination techniques.
24. Relative Lymphocytosis with Variant
Lymphocyte Morphology
• Miscellaneous Disorders
Lymphopenia and neutropenia
10% of patients with thyrotoxicosis have
neutropenia and relative lymphocytosis. The
blood probably due to disturbance of
adrenocortical function.
25. Relative Lymphocytosis with Normal
Lymphocyte Morphology
• Neutropenia
Wide variety of conditions in which the absolute
number of neutrophils decreases, leaving relative
lymphocytosis in which lymphocyte morphology is
normal.