Nano Fusion Technologies is a Japanese company that develops micro electro-osmotic pumps and microfluidic technologies. Their objective is to offer micro fluid delivery solutions to advance bioscience and microelectronics. Their major technology is micro electro-osmotic pumps, which use an electric field to generate electro-osmotic flow and pump liquids through porous ceramic discs. They provide various pump models covering a wide range of flow rates and pressures, as well as evaluation kits and a PWM power source for testing pumps. They also develop pump-on-chip technologies that directly integrate pumps onto microfluidic chips.

1. Introduction to
micro Electro-osmotic (EO) pumps
& micro-fluidic technologies
April 2009
Nano Fusion Technologies, Inc.
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2. Nano Fusion Technologies, Inc.
Founders and Managements
 Tihiro Ohkawa (Founder)
 Masana Nishikawa (Founder)
 Ichiro Yanagisawa (CEO/CTO)
 Masataka Ogawa (VP, Business Development)
Major Shareholders (Founders & VCs)
 Yasuda Enterprise Development Co., Ltd (Japan)
 Conduit Ventures Limited (UK)
 4 Japanese VCs
Founded: March 15, 2004
Capital: 229,750,000 yen
Headquarters: 2-21-7, Naka-cho, Meguro-ku, Tokyo, Japan
Laboratory: B-M202 Collaborative Research Bldg. (CCR)
Institute of Industrial Science, the University of Tokyo
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3. Objective
NFT Objective:
Offer micro fluid delivery solutions with unique micro pumps and
micro-fluidic control devices to advance bio-science and micro-
electronics functions.
Major Technologies:
Micro electro-osmotic pump (Micro EO pumps)

Micro-fluidic control devices and systems

Application Area:
Bio-Science, Environmental and Chemical Applications

mTAS / Analytical device
Pipetting / Dispensing
Drug delivery (infusion)
Micro Electronics

Micro Fuel Cell & others
Industrial (lubrication, etc.)
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4. Principle of micro EO pump
Liquid - Electro-osmotic Pump - Inside ceramic disc
Ceramic particle Liquid
Liquid
Porous
Electric field
ceramic EOF
disc
Voltage
(dielectric
Porous
material)
material
Electrodes
Nozzle Pumping
Electro-osmotic Flow (EOF)-generating mechanism Interface Liquid
I. Negative charges on the dielectric material surface.
II. Excessive positive charges generated in the liquid
Flow
Electric field
near the liquid-ceramics interface.
III. The applied electric field would force the positively
charged liquid layer to flow, that drags the whole
liquid.
Electro-osmotic flow (EOF) Dielectric material (Ceramics)
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5. Features of Micro EO pump
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6. Features of Micro EO pump
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7. Typical pump performances
Flow Rate (mL/min) Pressure (kPa)
10-1 102 103 102 103
1 10 1 10
Overall Coverage
RP3-N1/N2
RP5A-RL-N311/N610
RP5A-SP-N311/N610
7B-SP-N311/N610
LFHP
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8. RP5A-RL (evaluation sample)
RP5A-RL-N311 RP5A-RL-N610
Model
1)
Pumping principle Electro-osmosis
Max.Flow rate2) 110μ L/min 190μ L/min
3) @DC 24V 5)
140 kPa 110 kPa
Max.Pressure
13 mW 100mW
Power
4)
consumption
Working liquid Methanol De-ionized water
Polypropylene(Body・Nozzle・Reservior)
Material
Diameter 8mm,length 3.5mm(Body)
Size
Reservior capacity 1.2mL
0.5g
Weight
Performance of RP5A-RL-N311 Performance of RP5A-RL-N610
160 300
140
250
120
200
Flow rate (μL/min)
Flow rate (μL/min)
100
30V
80 150 30V
24V
24V
60
18V 100 18V
40
12V 12V
50
20
0 0
0 50 100 150 200 0 50 100 150
Back pressure (kPa) Back pressure (kPa)
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9. LFHP (evaluation sample)
- For high pressure or extremely low flow rate -
Performance of LFHP
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1) High pressure performance up to 2,000kPa (at 60 Volts)
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2) Extremely low flow rate (around 100 nL/min) 7 Working liquid:
Flow rate (μL/min)
3) Standard fitting design compatible with Upchurch fitting 6 De-ionized water
4) Extendable reservoir design 5
60V
4
3
LFHP
Model
2
24V
Pumping principle1) Electro-osmosis 1
8μL/min
Max.Flow rate2) 0
0 1000 2000 3000
Max.Pressure3) @DC 60V5) 2000kPa
Back pressure (kPa)
Power consumption4) 15mW
De-ionized water
Working liquid
Material PPS
Body size Diameter 10.3mm,length 27.3mm
Nozzle port Upchurch NanoPort Assemblies
F-333Nx
Nozzle fitting Upchurch NanoPort Assemblies
N-333-01
Weight 1.8g
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10. PWM Power Source and Controller
for Micro EO Pump (PS-PWM-24)
Enable stable micro-flow
by micro Electro-osmotic pump
1) PWM power source
2) Easy operation from Personal Computer
3) Built-in Feedback Control Function
PS-PWM-24 PS-PWM4C-24
Model
Output voltage1) 24V max
Duty ratio 0～100%
Pulse-frequency Variable (10～2000Hz)
Resolution of voltage 6mV
Power supply USB bus power AC adaptor
Input 100-240V 50/60Hz 0.3A
Output DC5V 2A
Output current 50mA max 50mAmax/1 channel
Number of I/O channel 1 4
Size(W×D×H) 85×55×21mm 85×55×48mm
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11. Evaluation Kits for micro EO pump
Evaluation Kits for full EO pump testing

Include all necessary components (pumps, power supply, flow sensor,

reservoirs and so on)
Evaluation Kits for different flow rate ranges

Low flow/High pressure kits (EVK-001) 0.1-1.5μL/min (-2000kPa)

Middle flow kits (EVK-002) 5-50μL/min

High flow kits (EVK-003) 100-1000μL/min

Low flow/High pressure kits EVK-001 formation High flow kits EVK-003 formation
Power supply PS-PWM4C-24
Reservoir for RP20-SP
Connecter
Power supply PS-PWM-24
Reservoir
Filter
LFHP
Tube (Teflon)
RP20-SP
Connecter
Flow sensor LG16-0025A
Reservoir Flow sensor LG16-1000A
Tube (Teflon)
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12. EO Pumps on a Micro-Channel Chip
Basic Concept
 Direct mount on micro-channel chips (Pump-on-chip)
 Integrated pump (Reservoir-on-pump)
Less redundant space
Reservoir
Less sample fluid

Precise flow rate &

flow amount control
EO Pump
Micro-channel chip
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13. EO pump applications
for Microfluidics I
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14. EO pump applications
for Microfluidics II
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15. Thank You
Nano Fusion Technologies, Inc.
Headquarters: (Mailing address)
Nano Fusion Technologies, Inc.
2-21-7 Naka-cho, Meguro-ku, Tokyo 153-0065
JAPAN
e-mail: nft-info@nft-eop.co.jp
Website: http://www.nft-eop.co.jp/
Laboratory:
B-M202 Collaborative Research Bldg. (CCR)
Institute of Industrial Science, the University of Tokyo
4-6-1 Komaba, Meguro-ku, Tokyo 153-8505
JAPAN
TEL: +81-3-5452-6619 FAX: +81-3-5452-6620
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