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This newsletter from Animal Care Services provides updates on several topics: 1) The Mouse Models Core has created new transgenic and knockout mouse lines and is offering additional services like embryo collection and storage. 2) Construction is underway for a new Biomedical Sciences Building, with an expected move-in date of October 30, 2009. 3) "Special Services" fees will now be implemented for any work done by ACS outside of standard husbandry care, such as specialized diets or housing. 4) Several staff changes are announced, including new veterinarians, technicians of the year, and retirements.
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![Dr. August BattlesAttending VeterinarianDirector of Animal Care ServicesLindsay HochmanEditorHealth Science CenterCommunicore CB-159P.O. Box 100006Gainesville, FL 32610-0006Telephone: 352-392-2977Fax: 352-392-3766Dr. August BattlesAttending VeterinarianDirector of Animal Care ServicesChristine WasnerEditor <br />Animal Care Services<br />NEWSLETTER<br />Volume 9, Issue 3September 2009<br />Mouse Models Core Update<br />The UF ACS Mouse Models Core (MMC) has created numerous new transgenic lines, as well as new knockout mice over the past two years. At this point, transgenic mice can be created on a variety of backgrounds, and knockouts can be produced on a 129 or B6 background, depending on the ES cells used.<br />The Core also has begun to provide ancilliary services to facilitate UF investigators’ outside collaborations, colony archiving, and colony rescue. To this point, we regularly offer preimplantation embryo collection, mouse line reconstitution from frozen embryos or sperm, archiving mouse lines by embryo and sperm cryopreservation, and embryo transfer rederivation of disease-carrying mouse lines. ACS can now store all or a portion of researcher’s frozen mouse embryos and sperm in its own autofill, alarmed liquid nitrogen storage tank! <br />Another service that we are currently developing is colony rescue via ovary transplantation. We have successfully rescued a number of researcher’s most important mouse lines via IVF! <br />Request forms for any of these services can be<br />found on the web at:<br />http://acs.ufl.edu/MouseModelCore/index.shtml <br />Fees for any of these services can be found on the web in MyACS upon logging in, and looking under “Breeding Services.”<br />,[object Object]](https://image.slidesharecdn.com/newsletter2635/85/Newsletter-1-320.jpg)
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This document discusses animal birth control and sterilization methods for dogs. It describes non-surgical birth control methods like hormones and chemicals as well as surgical sterilization techniques like ovariohysterectomy and pediatric neutering. It provides details on implementing an Animal Birth Control program including catching, transporting, and housing street dogs, as well as necessary personnel and infrastructure. The goal of these programs is to humanely control the street dog population through sterilization and vaccination.
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Newsletter
- 3. The final phases of construction for the new Biomedical Sciences Building are underway and we are preparing for October 30, 2009 as a start date for the move into this new facility.
- 4. Please check back to our website and future newsletter for more details and information as it becomes available.
- 6. Husbandry Special Services- Any services provided by ACS personnel for the husbandry of animals which are beyond what is prescribed in the Standard Operating Procedures (SOP) that addresses standard housing and husbandry care for that animal species, i.e. non standard housing, excess or alternative bedding, toys, specialized diets or non-standard feeding schedules, or extra man-hours incurred for the performance of minor research specific tasks for the convenience of the PI. These costs must be reimbursed by the individual PI/protocol. Husbandry Special Services charges may also be the result of unique physiology or behavior of individual animals. For example, diabetic rodents frequently demand cage changes much more frequently than non-diabetic animals.
- 7. Veterinary Care Special Services (Protocol Support) - Any technical or professional services provided by ACS personnel for research associated activities involving UF research animals which are not covered by standard per diem charges. These may include collection of biological specimens, administration of test materials, clinical observation of objective physiological data, i.e. vital signs, observations or measurements, laboratory tests, body weight, or temperature determinations, data recording and or consolidation, etc.
- 8. Equipment Special Services- Any loan/use of ACS-owned equipment for research associated activities involving UF research animals which are not covered by standard per diem charges. This includes inhalation anesthetic machine use, vaporized hydrogen peroxide generator use, cleaning of non-ACS equipment such as investigator metabolic cage systems and incineration of large animal carcasses, cleaning and sanitizing non-ACS equipment, etc.
- 11. She works well independently and is relied on to ensure that clinical care is carried out throughout the facilities as the veterinarians directed. My Assistant Director of Operations describes Lauren as the “Gold Standard” of veterinary/husbandry interaction. When she goes into a facility she talks to the husbandry techs about their animals, does her tasks and checks, and then talks to the husbandry staff again about what she observed, what she did, and what they need to be looking for to help care for their animals. She understands the bonds between the husbandry technicians and the animals they care for.
- 13. Commercially-available tops are available to euthanize animals inside plastic rodent cages. These tops seal the enclosure and create inlet/outlet holes that can be connected to the supply of CO2. The simplest functional system is one that has a single hole through which a length of tubing connected to the regulator passes. The tubing may extend down to the bottom of the chamber. This system allows gas to enter the chamber while air is vented out through the small opening around the tubing at the top.
- 14. A large plastic or glass dessicator jar can be used if a two-holed rubber stopper is inserted in the top. The hose from a CO2 regulator is connected to a six-to-eight-inch piece of rigid plastic or stainless steel tubing that passes through one hole of the stopper and allows the carbon dioxide to be admitted at the bottom of the chamber. A three-inch piece of plastic tubing is passed through the other hole and is connected to a short length of hose which has an adjustable screw-type clamp placed to regulate the escape of air and carbon dioxide from the chamber. Before administering gas, the ground glass surfaces should be sealed with silicone grease.Please contact ACS if you have any questions about whether your chamber is acceptable. An ACS veterinarian will gladly visit the lab and offer a consultation.<br /> Chambers should be kept clean to minimize odors that might distress animals subsequently euthanized. Chambers may be lined with clean disposable materials to enhance sanitation. Disposable liners should be removed and sanitization and rinsing should occur between animals or groups of animals and when all euthanasia is completed for the day.<br />For details on specific methods of euthanasia, please see http://acs.ufl.edu/guidelines/Euthanasia.shtml and http://www.iacuc.ufl.edu/Changes%20made%207-21-09/Modified%20GuidelinesforNeonatalRodentEuthanasia%207-21-09.pdf .<br />Animal Care Services (ACS) publishes this newsletter to communicate with those who use or provide support to the animal care program at the University of Florida. This newsletter contains various items of importance to animal users. We use e-mail as our primary method of distributing the newsletter. Please print this newsletter and distribute it to members of your department. If you did not receive this newsletter by email and would like to be added to the email list, please send an email message to lhochman@ufl.edu with “Add to ACS Newsletter” as the subject. This and all past issues of the newsletter can be accessed at http://acs.ufl.edu/newsletter.shtml<br />