PSYPACT- Practicing Over State Lines May 2024.pptx
mTERF Gene family for abiotic stress in plants
1. CHOUDHARY CHARAN SINGH
UNIVERSITY MEERUT 250004
TOPIC- mTERF Gene family
Submitted by:
Devendra Kumar
Shreya baranwal
M.sc. Ag. 2nd SEM
Submitted to:
Dr. Vinay Panwar
Department of Genetic and
plant breeding
ccs university meerut
2. mTERF Gene Family -
Plant mitochondrial
transcription termination
factor gene family
3. Plant mitochondrial transcription termination factor
(mTERF) family regulates organellar gene expression
(OGE) and is functionally characterized in diverse.
however limited data are available in crops about its
function.
Only 60 mTERF have identify in barley
genome(hvmTERF).
4. hvmTERF gene family are categories in 9 subfamily.
hvmTERF classify based on phylogenetic analysis.
Halves of hvmTERF are belonging from subfamily IX.
Expression analysis suggested that many hvmTERF may play
important role in barley development(seedling,leaves,and
developing inflosence)& abiotic stress (cold, salt, metal ion
etc).
hvmTERF21 and hvmTERF23 were significant induced by
various stresses or phytoharmone treatment.
5. mTERF protein family were firstly characterized in animal
mitochondria were involved in mitochondrial transcription,
translation, DNA Replication.
In a study of comprehensive database search yielded 31
potential mTERF genes in maize (Zea mays L.).
Its 31 mTERF genes are classify in IX main group based on
phytogenic analysis.
IXth group represents the mitochondrial and species-specific
clade that diverge from other groups.
6. Tendom and segmental duplication both contributed to
expension of the mTERF gene family in maize genome.
Comprehensive expression Analysis of these genes using
microarray data and RNA sequence data Revealed that these
genes exhibit a variety of expression patterns.
After conducting of many environmental experiments at the
stage of seedling eg: exposed to light/dark, salts and plant
harmone etc. It is revealed that various important roles of
maize mTERF genes in light acclimilation and stress related
response.
7. After experiments on maize and barley many experiments
conducted in the rice crop and V14 mTERF gene was
identified by fine mapping in rice.
Real time PCR indicates that V14 mTERF encodes a
chloroplastic protein V14 expression lacks thermo-sensitivity.
Based on research data revealed a critical role of V14 In
chloroplast development.
SOLDAT10, the first mTERF characterised in Higher plant
Participate in stress acclimilation response and affects the
abundance of 16s and 23s rRNA and clp p protease mRNA in
chloroplast.
8. V14 is a chloroplastic protein criticallto early stage of leaf
development.
V14 locus have 162kb region on chromosome 7.
This V14 mutant develops albinism in the first two true leaves
at 25˚C and returns green there after.