Monoclonal antibodies
•Download as PPTX, PDF•
3 likes•135 views
Monoclonal antibodies are laboratory-produced molecules that can bind to substances in the body like viruses, bacteria, or cancer cells. They are made by fusing a normal antibody-producing cell with a tumor cell in the laboratory, allowing the hybrid cell to produce unlimited quantities of identical antibodies that are specific to one substance. These antibodies can be used to detect, prevent, or treat diseases like cancer, infections, and autoimmune disorders.
Report
Share
Report
Share
Recommended
Monoclonal antibodies
1. Monoclonal antibodies are antibodies that are clones of a unique parent cell and bind to the same epitope of an antigen.
2. They are produced through cell fusion between antibody-producing B cells from immunized animals and myeloma cells, forming a hybridoma that produces the monoclonal antibody.
3. The monoclonal antibodies are screened and cloned to produce large quantities of identical antibodies directed against a specific antigen.
Bacterial vaccines
The document discusses different types of bacterial vaccines. It begins by introducing active and passive immunity. Active immunity is induced by vaccines made from bacteria or their products, while passive immunity provides preformed antibodies from another source. The main types of bacterial vaccines discussed are whole-organism attenuated or killed vaccines, purified macromolecule vaccines like polysaccharides or toxoids, and subunit vaccines. Specific examples provided include vaccines for cholera, plague, typhus, pertussis, tetanus, diphtheria, hepatitis B, and human papillomavirus. Memory cells and immunological memory play an important role depending on the incubation period of the pathogen.
Toxoid Vaccines
Killed bacterial vaccines against cholera, plague, typhus, and Q fever contain whole killed bacteria and are used to protect those exposed. Live attenuated bacterial vaccines like BCG against tuberculosis use live but weakened Mycobacterium bovis. Toxoid vaccines for diphtheria and tetanus contain inactivated toxins that have lost disease-causing ability but retained immunogenicity. Purified protein vaccines such as the acellular pertussis vaccine recommended for all children contain purified bacterial proteins. Capsular polysaccharide vaccines for pneumonia, meningitis, and H. influenzae conjugate the polysaccharide to a carrier protein to enhance the antibody response.
Immunoblotting techniques-ELISA, Western blotting, Southern blotting
Pharmaceutical Biotechnology, BP605T, Western blotting, Southern blotting, ELISA, Immunoblotting techniques, nitrocellulose membrane, horse-radish peroxidase, radioisotope, autoradiography, enzyme-substrate reaction, gel electrophoresis, Bovine serum albumin, preparation of gel for gel electrophoresis, TAE, TBE, SDS-PAGE electrophoresis, blotting techniques, polyacrylamide gel, PVDF, TBS-T, chemiluminescence, Antigen, antibody, spectrophotometer
Monoclonal antibodies
Monoclonal antibodies are monospecific and made by identical immune cells that are clones of a unique parent cell. They bind to the same epitope. Monoclonal antibodies are produced through the fusion of myeloma cells and immune spleen cells, followed by culturing, cloning, and selection of hybridoma cells. They are useful for purification of drugs, imaging targets, fighting infectious diseases and bioterrorism through their ability to bind specifically to antigens. Common applications include treating cancer, transplant rejection, and allergic asthma.
Bacterial vaccines
This document discusses the preparation of bacterial vaccines. It describes the key steps involved in producing killed bacterial vaccines such as selection of the bacterial strain, growth of bacteria in culture, inactivation of the bacteria through heat or chemicals, standardization, formulation, and storage. The document also discusses the preparation of live attenuated bacterial vaccines, using the BCG vaccine as an example. Key steps for BCG preparation include attenuation of the bacteria by repeated subculture, growth in liquid medium, harvesting and freeze drying the bacteria, and standardization based on viable cell counts.
monoclonal antibodies
Monoclonal antibodies are identical antibodies produced by a single clone of B cells or hybridomas. They can be used for diagnostic tests and therapy. In 1975, Köhler and Milstein developed the technique of fusing myeloma cells with spleen cells from immunized mice to generate hybridomas that produce monoclonal antibodies. This provided an unlimited supply of identical antibodies against specific antigens. Monoclonal antibodies have various applications such as diagnostic tests, purification of substances, and cancer treatment when conjugated to toxins or radioisotopes. However, they can cause side effects like allergic reactions, vomiting, and diarrhea when used intravenously.
Traditional vaccine preparation
This document discusses different types of traditional vaccines, including inactivated, live attenuated, toxoid, and subunit vaccines. It provides details on how each type is prepared, such as using heat or chemicals to kill pathogens for inactivated vaccines and weakening live viruses for live attenuated vaccines. Both advantages and disadvantages are described for each approach. Specific examples like the polio, diphtheria, tetanus, and influenza vaccines are examined in more depth. The history of vaccine development from Lady Mary Wortley Montagu's variolation technique to Louis Pasteur's first live attenuated vaccine for chicken cholera is also reviewed.
Recommended
Monoclonal antibodies
1. Monoclonal antibodies are antibodies that are clones of a unique parent cell and bind to the same epitope of an antigen.
2. They are produced through cell fusion between antibody-producing B cells from immunized animals and myeloma cells, forming a hybridoma that produces the monoclonal antibody.
3. The monoclonal antibodies are screened and cloned to produce large quantities of identical antibodies directed against a specific antigen.
Bacterial vaccines
The document discusses different types of bacterial vaccines. It begins by introducing active and passive immunity. Active immunity is induced by vaccines made from bacteria or their products, while passive immunity provides preformed antibodies from another source. The main types of bacterial vaccines discussed are whole-organism attenuated or killed vaccines, purified macromolecule vaccines like polysaccharides or toxoids, and subunit vaccines. Specific examples provided include vaccines for cholera, plague, typhus, pertussis, tetanus, diphtheria, hepatitis B, and human papillomavirus. Memory cells and immunological memory play an important role depending on the incubation period of the pathogen.
Toxoid Vaccines
Killed bacterial vaccines against cholera, plague, typhus, and Q fever contain whole killed bacteria and are used to protect those exposed. Live attenuated bacterial vaccines like BCG against tuberculosis use live but weakened Mycobacterium bovis. Toxoid vaccines for diphtheria and tetanus contain inactivated toxins that have lost disease-causing ability but retained immunogenicity. Purified protein vaccines such as the acellular pertussis vaccine recommended for all children contain purified bacterial proteins. Capsular polysaccharide vaccines for pneumonia, meningitis, and H. influenzae conjugate the polysaccharide to a carrier protein to enhance the antibody response.
Immunoblotting techniques-ELISA, Western blotting, Southern blotting
Pharmaceutical Biotechnology, BP605T, Western blotting, Southern blotting, ELISA, Immunoblotting techniques, nitrocellulose membrane, horse-radish peroxidase, radioisotope, autoradiography, enzyme-substrate reaction, gel electrophoresis, Bovine serum albumin, preparation of gel for gel electrophoresis, TAE, TBE, SDS-PAGE electrophoresis, blotting techniques, polyacrylamide gel, PVDF, TBS-T, chemiluminescence, Antigen, antibody, spectrophotometer
Monoclonal antibodies
Monoclonal antibodies are monospecific and made by identical immune cells that are clones of a unique parent cell. They bind to the same epitope. Monoclonal antibodies are produced through the fusion of myeloma cells and immune spleen cells, followed by culturing, cloning, and selection of hybridoma cells. They are useful for purification of drugs, imaging targets, fighting infectious diseases and bioterrorism through their ability to bind specifically to antigens. Common applications include treating cancer, transplant rejection, and allergic asthma.
Bacterial vaccines
This document discusses the preparation of bacterial vaccines. It describes the key steps involved in producing killed bacterial vaccines such as selection of the bacterial strain, growth of bacteria in culture, inactivation of the bacteria through heat or chemicals, standardization, formulation, and storage. The document also discusses the preparation of live attenuated bacterial vaccines, using the BCG vaccine as an example. Key steps for BCG preparation include attenuation of the bacteria by repeated subculture, growth in liquid medium, harvesting and freeze drying the bacteria, and standardization based on viable cell counts.
monoclonal antibodies
Monoclonal antibodies are identical antibodies produced by a single clone of B cells or hybridomas. They can be used for diagnostic tests and therapy. In 1975, Köhler and Milstein developed the technique of fusing myeloma cells with spleen cells from immunized mice to generate hybridomas that produce monoclonal antibodies. This provided an unlimited supply of identical antibodies against specific antigens. Monoclonal antibodies have various applications such as diagnostic tests, purification of substances, and cancer treatment when conjugated to toxins or radioisotopes. However, they can cause side effects like allergic reactions, vomiting, and diarrhea when used intravenously.
Traditional vaccine preparation
This document discusses different types of traditional vaccines, including inactivated, live attenuated, toxoid, and subunit vaccines. It provides details on how each type is prepared, such as using heat or chemicals to kill pathogens for inactivated vaccines and weakening live viruses for live attenuated vaccines. Both advantages and disadvantages are described for each approach. Specific examples like the polio, diphtheria, tetanus, and influenza vaccines are examined in more depth. The history of vaccine development from Lady Mary Wortley Montagu's variolation technique to Louis Pasteur's first live attenuated vaccine for chicken cholera is also reviewed.
Edible vaccines
Edible vaccines involve introducing selected genes into plants to produce antigens. When consumed, the antigens are delivered through the digestive tract to immune cells to produce an immune response. Clinical trials show edible potatoes expressing antigens from ETEC, Norwalk virus, and hepatitis B produced protective antibody levels in participants. Edible vaccines could provide a low-cost, heat-stable, easy to administer option for vaccination programs in developing countries. However, public resistance to genetically modified foods and risks of transgenic contamination limit the future of edible vaccines.
MONOCLONAL ANTIBODIES
This document discusses monoclonal antibodies (mAb), including their production through hybridoma technology. Some key points:
- mAb are identical antibodies produced by identical immune cells cloned from a single parent cell. They were first produced in 1975 using cell fusions.
- The hybridoma technique involves immunizing an animal, fusing spleen cells that produce antibodies with myeloma cells to form a hybridoma, and using HAT medium to select antibody-producing hybridomas.
- Applications of mAb include research, diagnostics, and therapies for cancer, autoimmune disorders, and more. Variants like chimeric and humanized mAb reduce immunogenicity.
immunoblotting techniques
Immunoblotting assays such as Western blotting allow detection of specific proteins in complex mixtures by separating proteins by gel electrophoresis, transferring them to a membrane, and using antibodies to identify target proteins on the membrane. The presentation provides details on the key steps of tissue preparation, gel electrophoresis, protein transfer, blocking, detection using labeled antibodies, analysis, and applications of immunoblotting assays like Western blotting.
DNA Vaccine
DNA vaccines work by injecting DNA encoding antigens from pathogens. The host cells use this DNA to produce antigens, which are then displayed on the cell surface and trigger both humoral and cellular immune responses. DNA vaccines offer advantages over traditional vaccines like avoiding infectious organisms, not requiring refrigeration, and stimulating both arms of the immune system. They have shown protection against diseases in animal studies and have potential applications for influenza, hepatitis B, HIV, and malaria vaccines. However, DNA vaccines also have disadvantages like weak immune responses in humans.
Vaccine preparation part 1 ppt
The document outlines the general methods used for vaccine production, which includes 5 main steps: 1) generating the antigen by growing pathogens, 2) releasing and isolating the antigen from growth materials, 3) purifying the antigen using various separation techniques, 4) adding other components like adjuvants and preservatives, 5) packaging the vaccine for distribution while protecting it and the environment. Vaccines work by improving immunity but may not guarantee full protection against disease.
Monoclonal Antibodies
Monoclonal antibodies are produced by injecting mice with antigens to trigger antibody production. The mouse spleen is then harvested and mixed with non-immunized mouse cells in culture to produce hybridomas that secrete identical antibodies targeting specific antigens. Monoclonal antibodies have applications in cancer treatment, cleaning bone before transplantation, treating certain immune disorders, studying medicine, and targeting parasites or antigens on external tissues for vaccine production. They are also used to treat diseases like Chron's disease, rheumatoid arthritis, prevent organ transplant rejection, and treat various cancers.
Nanotechnology and drug delivery
Nanotechnology drug delivery applications occur through the use of designed nanomaterials as well as forming delivery systems from nanoscale molecules such as liposomes. ... Improve the ability to deliver drugs that are poorly water soluble. Provide site-specific targeting to reduce drug accumulation within healthy tissue.Drug delivery systems (DDSs) are developed to deliver the required amount of drugs effectively to appropriate target sites and to maintain the desired drug levels. Research in newer DDS is being carried out in liposomes, nanoparticles, niosomes, transdermal drug delivery, implants, microencapsulation, and polymers.
Recent Development In Nanovaccine 1
Recent developments in nanovaccine. Nanovaccines can be designed and manufactured to improve health at the molecular level. Nanovaccines offer different routes of administration and include delivery via nanobeads, polymeric nanoparticles, nanoemulsions, and viral vectors. They have potential advantages like stronger immune responses, stability without refrigeration, and pain-free delivery. However, challenges remain regarding production costs, safety, and reproducibility. Future prospects include using nanomaterials to deliver vaccines for diseases like HIV, influenza, and Alzheimer's.
Bacterial vaccines
There are several types of bacterial vaccines that work through different mechanisms of the immune system. Active immunization stimulates the body's own immune response by introducing an antigen from the bacteria. This induces long-term immunity through memory B and T cells. Passive immunization provides immediate, short-term protection by transferring pre-formed antibodies. Common bacterial vaccines include those targeting Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, diphtheria, tetanus, pertussis, Lyme disease, anthrax, tuberculosis, and typhoid fever. These vaccines contain inactivated toxins, purified proteins, capsular polysaccharides, or live attenuated bacterial strains.
Mabjeevan 141027001544-conversion-gate01
Monoclonal antibodies (mAbs) are identical antibodies produced by a single clone of B cells or hybridoma cell line. Paul Ehrlich first described antibodies as "magic bullets" in search of toxins. Key developments included methods to isolate hybrid cell lines producing mAbs. mAbs can be murine, chimeric, humanized, or human. They have diagnostic applications like pregnancy tests and therapeutic uses like treating cancer, transplants, and autoimmune disorders. Common side effects include allergic reactions and infusion reactions. mAbs have revolutionized biotechnology and improved human health.
Monoclonal antibodies
Monoclonal antibodies are antibodies produced by a single clone of cells that make one specific antibody. They are homogeneous, specific, and do not vary between batches as they bind to a single antigen. Monoclonal antibodies have applications in diagnostics, such as pregnancy tests, and therapeutics, such as treatments for cancer and organ transplantation. They are produced through cell fusion methods like roller bottle culture or bioreactor culture.
Nanotechnology in Drug Delivery
These slides use concepts from my (Jeff Funk) course entitled analyzing hi-tech opportunities to show how nanotechnology for drug deliver is becoming economically feasible.
Bacterial vaccines
The document discusses the history and development of vaccines. It begins by defining what a vaccine is and how it stimulates immunity. It then discusses key events in vaccine development like Edward Jenner using cowpox to provide smallpox protection in 1796. The document outlines different types of vaccines like live attenuated, killed, toxoid, subunit, conjugate, and experimental DNA vaccines. It provides examples of vaccines for different diseases and discusses concepts like valence, adjuvants, storage and administration.
Microbiology Assays - Pharmaceutical microbiology (Second year b.pharm) (3rd ...
Prof.Mr.Kiran K. Shinde (M.Pharm), Assistant professor (VNIPRC)
Pharmaceutical microbiology (Second year b.pharm) (3rd semester)
Introduction
Methods Of Different microbiological assays
Principles of Assays with Procedure
Methods For Standardization of
1. Antibiotics
2. Vitamins
3. Amino Acids
Assessment of new Antibiotic
Monoclonal antibodies [autosaved]
Monoclonal antibodies are derived from a single B cell clone and offer a reproducible and potentially inexhaustible supply of antibodies with exquisite specificity. They have enabled the development of more secure immunoassay systems and powerful clinical diagnostic tests. Monoclonal antibodies can be classified as naked antibodies or conjugated antibodies attached to drugs, toxins, or radioactive atoms to selectively target diseases. They have various mechanisms of action including blocking target antigens and inducing cytotoxicity, and are used to treat cancers, autoimmune disorders, and transplant rejection.
Different Types of Vaccine
The document discusses different types of vaccines, including live attenuated vaccines, inactivated vaccines, subunit vaccines, recombinant viral protein vaccines, synthetic peptide vaccines, anti-idiotype antibodies vaccines, and DNA vaccines. It provides details on how each type of vaccine is produced and advantages and disadvantages of each approach. Key points covered include methods used to attenuate viruses for live vaccines, challenges with developing inactivated vaccines, use of recombinant DNA technology to produce subunit and viral protein vaccines, and potential of DNA vaccines which directly inject DNA coding for antigens.
MONOCLONAL ANTIBODIES: Preparation & Application
This document discusses monoclonal antibodies, including their preparation and applications. It begins with an introduction to antibodies and monoclonal antibodies. It then describes the preparation process, which involves immunizing an animal, isolating B cells, fusing them with myeloma cells to form hybridomas, screening clones, and growing selected clones to produce monoclonal antibodies. The document outlines major applications of monoclonal antibodies in diagnosis using techniques like immunoassays and imaging, as well as their use as therapeutic agents and in protein purification.
MONOCLONAL ANTIBODIES
This document discusses and compares polyclonal and monoclonal antibodies. Polyclonal antibodies are derived from different B cell lineages and can have batch-to-batch variation, making them less suitable for clinical diagnostic tests. Monoclonal antibodies are derived from a single B cell clone and have greater homogeneity, specificity, and ability to produce unlimited quantities of antibody, enabling their use in diagnostic tests. The document also outlines methods for generating recombinant antibodies and the various therapeutic and diagnostic applications of monoclonal antibodies.
Application of rDNA in the Production of Interferon, Hepatitis Vaccines & Ins...
1) The document discusses the application of recombinant DNA technology in the production of interferon, hepatitis B vaccine, and insulin.
2) Interferon is produced through recombinant DNA by isolating the human interferon gene and inserting it into bacterial plasmids, allowing bacteria to express the gene and produce interferon protein.
3) Insulin is similarly produced by isolating the human insulin gene, inserting it into bacterial plasmids, and having bacteria express the gene to synthesize insulin protein.
Monoclonal antibodies
This presentation aims to explore about the approach on monoclonal antibodies with sequential demystification of it's various aspect.
Histololgy of Female Reproductive System.pptx
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
More Related Content
What's hot
Edible vaccines
Edible vaccines involve introducing selected genes into plants to produce antigens. When consumed, the antigens are delivered through the digestive tract to immune cells to produce an immune response. Clinical trials show edible potatoes expressing antigens from ETEC, Norwalk virus, and hepatitis B produced protective antibody levels in participants. Edible vaccines could provide a low-cost, heat-stable, easy to administer option for vaccination programs in developing countries. However, public resistance to genetically modified foods and risks of transgenic contamination limit the future of edible vaccines.
MONOCLONAL ANTIBODIES
This document discusses monoclonal antibodies (mAb), including their production through hybridoma technology. Some key points:
- mAb are identical antibodies produced by identical immune cells cloned from a single parent cell. They were first produced in 1975 using cell fusions.
- The hybridoma technique involves immunizing an animal, fusing spleen cells that produce antibodies with myeloma cells to form a hybridoma, and using HAT medium to select antibody-producing hybridomas.
- Applications of mAb include research, diagnostics, and therapies for cancer, autoimmune disorders, and more. Variants like chimeric and humanized mAb reduce immunogenicity.
immunoblotting techniques
Immunoblotting assays such as Western blotting allow detection of specific proteins in complex mixtures by separating proteins by gel electrophoresis, transferring them to a membrane, and using antibodies to identify target proteins on the membrane. The presentation provides details on the key steps of tissue preparation, gel electrophoresis, protein transfer, blocking, detection using labeled antibodies, analysis, and applications of immunoblotting assays like Western blotting.
DNA Vaccine
DNA vaccines work by injecting DNA encoding antigens from pathogens. The host cells use this DNA to produce antigens, which are then displayed on the cell surface and trigger both humoral and cellular immune responses. DNA vaccines offer advantages over traditional vaccines like avoiding infectious organisms, not requiring refrigeration, and stimulating both arms of the immune system. They have shown protection against diseases in animal studies and have potential applications for influenza, hepatitis B, HIV, and malaria vaccines. However, DNA vaccines also have disadvantages like weak immune responses in humans.
Vaccine preparation part 1 ppt
The document outlines the general methods used for vaccine production, which includes 5 main steps: 1) generating the antigen by growing pathogens, 2) releasing and isolating the antigen from growth materials, 3) purifying the antigen using various separation techniques, 4) adding other components like adjuvants and preservatives, 5) packaging the vaccine for distribution while protecting it and the environment. Vaccines work by improving immunity but may not guarantee full protection against disease.
Monoclonal Antibodies
Monoclonal antibodies are produced by injecting mice with antigens to trigger antibody production. The mouse spleen is then harvested and mixed with non-immunized mouse cells in culture to produce hybridomas that secrete identical antibodies targeting specific antigens. Monoclonal antibodies have applications in cancer treatment, cleaning bone before transplantation, treating certain immune disorders, studying medicine, and targeting parasites or antigens on external tissues for vaccine production. They are also used to treat diseases like Chron's disease, rheumatoid arthritis, prevent organ transplant rejection, and treat various cancers.
Nanotechnology and drug delivery
Nanotechnology drug delivery applications occur through the use of designed nanomaterials as well as forming delivery systems from nanoscale molecules such as liposomes. ... Improve the ability to deliver drugs that are poorly water soluble. Provide site-specific targeting to reduce drug accumulation within healthy tissue.Drug delivery systems (DDSs) are developed to deliver the required amount of drugs effectively to appropriate target sites and to maintain the desired drug levels. Research in newer DDS is being carried out in liposomes, nanoparticles, niosomes, transdermal drug delivery, implants, microencapsulation, and polymers.
Recent Development In Nanovaccine 1
Recent developments in nanovaccine. Nanovaccines can be designed and manufactured to improve health at the molecular level. Nanovaccines offer different routes of administration and include delivery via nanobeads, polymeric nanoparticles, nanoemulsions, and viral vectors. They have potential advantages like stronger immune responses, stability without refrigeration, and pain-free delivery. However, challenges remain regarding production costs, safety, and reproducibility. Future prospects include using nanomaterials to deliver vaccines for diseases like HIV, influenza, and Alzheimer's.
Bacterial vaccines
There are several types of bacterial vaccines that work through different mechanisms of the immune system. Active immunization stimulates the body's own immune response by introducing an antigen from the bacteria. This induces long-term immunity through memory B and T cells. Passive immunization provides immediate, short-term protection by transferring pre-formed antibodies. Common bacterial vaccines include those targeting Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, diphtheria, tetanus, pertussis, Lyme disease, anthrax, tuberculosis, and typhoid fever. These vaccines contain inactivated toxins, purified proteins, capsular polysaccharides, or live attenuated bacterial strains.
Mabjeevan 141027001544-conversion-gate01
Monoclonal antibodies (mAbs) are identical antibodies produced by a single clone of B cells or hybridoma cell line. Paul Ehrlich first described antibodies as "magic bullets" in search of toxins. Key developments included methods to isolate hybrid cell lines producing mAbs. mAbs can be murine, chimeric, humanized, or human. They have diagnostic applications like pregnancy tests and therapeutic uses like treating cancer, transplants, and autoimmune disorders. Common side effects include allergic reactions and infusion reactions. mAbs have revolutionized biotechnology and improved human health.
Monoclonal antibodies
Monoclonal antibodies are antibodies produced by a single clone of cells that make one specific antibody. They are homogeneous, specific, and do not vary between batches as they bind to a single antigen. Monoclonal antibodies have applications in diagnostics, such as pregnancy tests, and therapeutics, such as treatments for cancer and organ transplantation. They are produced through cell fusion methods like roller bottle culture or bioreactor culture.
Nanotechnology in Drug Delivery
These slides use concepts from my (Jeff Funk) course entitled analyzing hi-tech opportunities to show how nanotechnology for drug deliver is becoming economically feasible.
Bacterial vaccines
The document discusses the history and development of vaccines. It begins by defining what a vaccine is and how it stimulates immunity. It then discusses key events in vaccine development like Edward Jenner using cowpox to provide smallpox protection in 1796. The document outlines different types of vaccines like live attenuated, killed, toxoid, subunit, conjugate, and experimental DNA vaccines. It provides examples of vaccines for different diseases and discusses concepts like valence, adjuvants, storage and administration.
Microbiology Assays - Pharmaceutical microbiology (Second year b.pharm) (3rd ...
Prof.Mr.Kiran K. Shinde (M.Pharm), Assistant professor (VNIPRC)
Pharmaceutical microbiology (Second year b.pharm) (3rd semester)
Introduction
Methods Of Different microbiological assays
Principles of Assays with Procedure
Methods For Standardization of
1. Antibiotics
2. Vitamins
3. Amino Acids
Assessment of new Antibiotic
Monoclonal antibodies [autosaved]
Monoclonal antibodies are derived from a single B cell clone and offer a reproducible and potentially inexhaustible supply of antibodies with exquisite specificity. They have enabled the development of more secure immunoassay systems and powerful clinical diagnostic tests. Monoclonal antibodies can be classified as naked antibodies or conjugated antibodies attached to drugs, toxins, or radioactive atoms to selectively target diseases. They have various mechanisms of action including blocking target antigens and inducing cytotoxicity, and are used to treat cancers, autoimmune disorders, and transplant rejection.
Different Types of Vaccine
The document discusses different types of vaccines, including live attenuated vaccines, inactivated vaccines, subunit vaccines, recombinant viral protein vaccines, synthetic peptide vaccines, anti-idiotype antibodies vaccines, and DNA vaccines. It provides details on how each type of vaccine is produced and advantages and disadvantages of each approach. Key points covered include methods used to attenuate viruses for live vaccines, challenges with developing inactivated vaccines, use of recombinant DNA technology to produce subunit and viral protein vaccines, and potential of DNA vaccines which directly inject DNA coding for antigens.
MONOCLONAL ANTIBODIES: Preparation & Application
This document discusses monoclonal antibodies, including their preparation and applications. It begins with an introduction to antibodies and monoclonal antibodies. It then describes the preparation process, which involves immunizing an animal, isolating B cells, fusing them with myeloma cells to form hybridomas, screening clones, and growing selected clones to produce monoclonal antibodies. The document outlines major applications of monoclonal antibodies in diagnosis using techniques like immunoassays and imaging, as well as their use as therapeutic agents and in protein purification.
MONOCLONAL ANTIBODIES
This document discusses and compares polyclonal and monoclonal antibodies. Polyclonal antibodies are derived from different B cell lineages and can have batch-to-batch variation, making them less suitable for clinical diagnostic tests. Monoclonal antibodies are derived from a single B cell clone and have greater homogeneity, specificity, and ability to produce unlimited quantities of antibody, enabling their use in diagnostic tests. The document also outlines methods for generating recombinant antibodies and the various therapeutic and diagnostic applications of monoclonal antibodies.
Application of rDNA in the Production of Interferon, Hepatitis Vaccines & Ins...
1) The document discusses the application of recombinant DNA technology in the production of interferon, hepatitis B vaccine, and insulin.
2) Interferon is produced through recombinant DNA by isolating the human interferon gene and inserting it into bacterial plasmids, allowing bacteria to express the gene and produce interferon protein.
3) Insulin is similarly produced by isolating the human insulin gene, inserting it into bacterial plasmids, and having bacteria express the gene to synthesize insulin protein.
Monoclonal antibodies
This presentation aims to explore about the approach on monoclonal antibodies with sequential demystification of it's various aspect.
What's hot (20)
Microbiology Assays - Pharmaceutical microbiology (Second year b.pharm) (3rd ...
Microbiology Assays - Pharmaceutical microbiology (Second year b.pharm) (3rd ...
Application of rDNA in the Production of Interferon, Hepatitis Vaccines & Ins...
Application of rDNA in the Production of Interferon, Hepatitis Vaccines & Ins...
Recently uploaded
Histololgy of Female Reproductive System.pptx
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
Post-Menstrual Smell- When to Suspect Vaginitis.pptx
Do you have abnormal smell after periods? It may be vaginitis. Do not worry, herbal medicine Fuyan Pill can help you get a cure.
Role of Mukta Pishti in the Management of Hyperthyroidism
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
Adhd Medication Shortage Uk - trinexpharmacy.com
The UK is currently facing a Adhd Medication Shortage Uk, which has left many patients and their families grappling with uncertainty and frustration. ADHD, or Attention Deficit Hyperactivity Disorder, is a chronic condition that requires consistent medication to manage effectively. This shortage has highlighted the critical role these medications play in the daily lives of those affected by ADHD. Contact : +1 (747) 209 – 3649 E-mail : sales@trinexpharmacy.com
Artificial Intelligence Symposium (THAIS)
Artificial Intelligence Symposium (THAIS). Parc Taulí. Sabadell
Ketone bodies and metabolism-biochemistry
This slide consists of all the topics of ketone . This can be used for exam purpose for writing about Diabetic keto acidosis etc . Thank you
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
It includes regulation of combination products and medical devices. FDA and industry liaisons.
Integrating Ayurveda into Parkinson’s Management: A Holistic Approach
Explore the benefits of combining Ayurveda with conventional Parkinson's treatments. Learn how a holistic approach can manage symptoms, enhance well-being, and balance body energies. Discover the steps to safely integrate Ayurvedic practices into your Parkinson’s care plan, including expert guidance on diet, herbal remedies, and lifestyle modifications.
Efficacy of Avartana Sneha in Ayurveda
Avartana Sneha is a unique method of Preparation of Sneha Kalpana in Ayurveda, mainly it is indicated for the Vataja rogas.
Recently uploaded (20)
Post-Menstrual Smell- When to Suspect Vaginitis.pptx
Post-Menstrual Smell- When to Suspect Vaginitis.pptx
Role of Mukta Pishti in the Management of Hyperthyroidism
Role of Mukta Pishti in the Management of Hyperthyroidism
Tests for analysis of different pharmaceutical.pptx
Tests for analysis of different pharmaceutical.pptx
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
Integrating Ayurveda into Parkinson’s Management: A Holistic Approach
Integrating Ayurveda into Parkinson’s Management: A Holistic Approach