This document summarizes a study on micropropagating passion fruit (Passiflora edulis) from leaf explants. It provides background on previous work culturing passion fruit explants and discusses the objective to micropropagate P. edulis from leaf explants. The materials and methods section outlines the explant collection and sterilization process, media preparation using MS media and cytokinins BAP and TDZ, and the process for bud induction, shoot elongation, rooting, and testing different hormone combinations and conditions. The conclusion discusses the economic importance of micropropagation for increasing passion fruit yields.

1. Micropropagation of Passion fruit:
Passiflora edulis
Presented by:
Md. Mobarok Karim
Reg:2013431024
Md. Abu Bakar Siddique
Reg:2013431025
Md. Mohsin Bapary
Reg:2013431026

2. Introduction:
• Vine species
• Family
• Native of tropical America

3. Flowers and fruits of passion fruit:
Passiflora fruitPassiflora fruit Passiflora flower

4. Introduction
• Fruit value
Serving size 170 g
Serving per container 1
Amount per serving 1 container
Calories 180
Calories from fat 50
%DV
Saturated fat 1 g 5%
Trans fat 0 g
Total fat 1.5 g 2%
Cholesterol 10 mg 3%
Sodium 100 mg 4%
Potassium 310 mg 9%
Carbohydrate 34 mg 11%
Fiber 0 g 0%
Sugar 29 g
Protein 7 g
Vitamin- A,C,D 20%,2%,20%
Calcium 25%

5. Background:
• N.O. Amugune, H.N.B Gopalan, B. Bytebier worked on Leaf
disc regeneration of Passiflora edulis and explant was cultured
on a modified Murashige and Skoog’s (MS) medium containing
BAP and KIN
• Beatriz Appeazzato Da Gloria, Maria Lucia Carneiro Vieira and
Maecelo Carnier Dornelas worked on in vitro organogenesis of
Passiflora edulis Sims f. Flavicarpa Deg., the passionfruit, leaf-
derived explants were cultured on media containing NAA or
BAP

6. Objective:
• Micropropagation of Passiflora edulis from
leaf explant

7. Materials and method:
• Explant from 60 day old plant
• Central part of the leafs
• Sterilization with ethanol,sodium
hypochlorite, Tween 20
Explants collection and sterilization:

8. Media preparation and growth condition:
• MS media
• Cytokinin : BAP and TDZ
BAP TDZ

9. Bud induction:
• MS media supplemented with BAP with or
without silver nitrate
• Different amount of BAP:0, 2.2, 4.4, 6.6 μmol/L
• MS media supplemented with TDZ with or
without silver nitrate
• Different amount of TDZ:0, 1.1, 2.2, 3.4 μmol/L

10. Shoot elongation and rooting:
• MSM supplement with 2.6 μmol L-1 GA3
• MSM supplement with coconut water (10%)
• ½ MSM salts
• Flasks should be capped with normal or vented lids
• MSM medium supplement with 4.9 μmol/L IBA for
rooting

11. BAP with or without silver nitrate:
Different combinations of hormones will be used for bud induction:

12. TDZ with or without silver nitrate:

13. Result and discussion:
• Probably the presence of 1.1μmolL/L TDZ and 23.5 μmol/L
AgNO3 will have the higher efficiency in shoot elongation when
vented lids will be used
• Reason behind this prediction

14. Conclusion:
• Passifloraceae family is economically very important family.
• If we gain our desired quality and yield by tissue culture method, it will be
our main agricultural product.
• We can earn foreign currency by exporting it’s fruit and their derivatives