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BHARATHIDASAN UNIVERSITY
TIRUCHIRAPPALLI-24
DEPARTMENT OF BIOTECHNOLOGY
MICRO ORGANISMS INVOLVED IN
ALCOHOL STEROL SEMISYNTHETIC
PENICILLIN
MICRO ORGANISMS
INVOLVE IN ALCOHOL
• Alcohol chemically ethanol has been produced
by fermentation for thousands of years.
• Yeast are the main fermenter and alcohol
producer in the production of wine ,beer and
other alcohol drinks.
• The main yeast species used is Saccharomyces
cerevisiae
• It ferments the sugars coming from different
sources eg ., grapes for wine ,barley for wine,to
alcohol and carbon dioxide.
• Certain yeast and bacteria are employed for
alcohol fermentation.
• The type of the organism chosen mostly
depends on the nature of the substrste used.
• Saccharomyces cerevsiae is the most
commonly used while among the bacteria ,
Zymomonas mobilis is the most frequently
employed for alcohol production
Microorganisms involved
YEAST
• Saccharomyces
cerevisiae
• S. ellipsoideous
• Kluyveromyces fragilis
Bacteria
Zymomonas mobilis
Candida pseudotropicalis
Candida utilis
Source of carbohydrate
Sugar,starch
Sugar,starch
Sugar,starch
Starch
Lactose,whey
Sulfite,waste liquor
Biosynthesis of ethanol
Starch
Hydrolysis
Glucose
Glycolysis
Pyruvate
co2 Pyruvate decarboxylase
Acedaldehyde
Alcohol dehydrogenase (NADH+H)
Ethanol
MICRO ORGANISMS
INVOLVE IN STEROL
• STEROL also known as steroid alcohols
tetracyclic triterpenoid lipids.
• They occur in plants, animals and fungi and
can also produced by some bacteria.
• Sterol production was first discovered in the
aerobic methanotroph Methylococcus
capsulatus
• Sterol biosynthesis also observed in a few
myxobacteria of the proteobacteria
• Two myxobacteria Stigmatella aurantica and
Cystobacter fuscus also produce
biosynthetically simple sterols .
Microorganism involved in
semisynthetic pencillin
• Penicillium chrysogenum or p.notatum is a species of
fungus in the genus penicillium involved in production
of semisynthetic penicillin.
• Microbial transformation in association with chemical
synthesis is routinely used for the commercial
production of semisynthetic penicillin
• The enzymztic cleavage of penicillin by penicillin
acylase into amino penicillanic acid is very important
reaction
• Pencillin G gets inactivated by its conversion to
benzylpenicilloic acid by the enzyme penicillinase(β
lactamase).
penicillinG
penicillin penicillinase
acylase (βlactamase)
Phenyl
Acetic acid
6-Aminopenicillanic Benzylpenicilloic
acid acid
chemical reaction
semisynthetic penicillin
Penicillium notatum
MICRO ORGANISMS INVOLVED IN ALCOHOL STEROL SEMISYNTHETIC PENICILLIN

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MICRO ORGANISMS INVOLVED IN ALCOHOL STEROL SEMISYNTHETIC PENICILLIN

  • 1. BHARATHIDASAN UNIVERSITY TIRUCHIRAPPALLI-24 DEPARTMENT OF BIOTECHNOLOGY MICRO ORGANISMS INVOLVED IN ALCOHOL STEROL SEMISYNTHETIC PENICILLIN
  • 2. MICRO ORGANISMS INVOLVE IN ALCOHOL • Alcohol chemically ethanol has been produced by fermentation for thousands of years. • Yeast are the main fermenter and alcohol producer in the production of wine ,beer and other alcohol drinks. • The main yeast species used is Saccharomyces cerevisiae • It ferments the sugars coming from different sources eg ., grapes for wine ,barley for wine,to alcohol and carbon dioxide.
  • 3. • Certain yeast and bacteria are employed for alcohol fermentation. • The type of the organism chosen mostly depends on the nature of the substrste used. • Saccharomyces cerevsiae is the most commonly used while among the bacteria , Zymomonas mobilis is the most frequently employed for alcohol production
  • 4. Microorganisms involved YEAST • Saccharomyces cerevisiae • S. ellipsoideous • Kluyveromyces fragilis Bacteria Zymomonas mobilis Candida pseudotropicalis Candida utilis Source of carbohydrate Sugar,starch Sugar,starch Sugar,starch Starch Lactose,whey Sulfite,waste liquor
  • 5. Biosynthesis of ethanol Starch Hydrolysis Glucose Glycolysis Pyruvate co2 Pyruvate decarboxylase Acedaldehyde Alcohol dehydrogenase (NADH+H) Ethanol
  • 6. MICRO ORGANISMS INVOLVE IN STEROL • STEROL also known as steroid alcohols tetracyclic triterpenoid lipids. • They occur in plants, animals and fungi and can also produced by some bacteria. • Sterol production was first discovered in the aerobic methanotroph Methylococcus capsulatus
  • 7. • Sterol biosynthesis also observed in a few myxobacteria of the proteobacteria • Two myxobacteria Stigmatella aurantica and Cystobacter fuscus also produce biosynthetically simple sterols .
  • 8. Microorganism involved in semisynthetic pencillin • Penicillium chrysogenum or p.notatum is a species of fungus in the genus penicillium involved in production of semisynthetic penicillin. • Microbial transformation in association with chemical synthesis is routinely used for the commercial production of semisynthetic penicillin • The enzymztic cleavage of penicillin by penicillin acylase into amino penicillanic acid is very important reaction • Pencillin G gets inactivated by its conversion to benzylpenicilloic acid by the enzyme penicillinase(β lactamase).
  • 9. penicillinG penicillin penicillinase acylase (βlactamase) Phenyl Acetic acid 6-Aminopenicillanic Benzylpenicilloic acid acid chemical reaction semisynthetic penicillin