This document provides information about meningitis, including its definition, causes, symptoms, diagnostic tests, and treatment. It defines meningitis as an inflammation of the membranes surrounding the brain and spinal cord. Bacterial, viral, fungal, and other physical/chemical agents can cause meningitis. Common symptoms include fever, headache, and neck stiffness. Diagnosis involves spinal fluid analysis, culture and sensitivity testing. Treatment involves antibiotics, antivirals, or antifungals depending on the cause, along with corticosteroids to reduce inflammation.
Lab diagnosis of Sexually transmitted Infections (STIs)Mostafa Mahmoud
This lecture was presented to the physicians dealing with the various infectious diseases specially in STIs in Riyadh Region, MOH. The lecture concentrates about the various methodology applied to diagnose STIs in the laboratory with the advantages and disadvantages of each. Hope to make benefits to all.
lecture for MBBS students
Rickettsia named after HOWARD
TAYLOR RICKETTS died of Typhus fever contracted during his studies
Discovered spotted fever rickettsia (1906)
Obligate intracellular parasite
Gram negative pleomorphic rods
Parasite of arthropods – fleas, lice, ticks and mites.
No Human to human transmission.
Rickettsia are transmitted to humans by the bite of infected arthropod vector.
Multiply at the site of entry and enter the blood stream.
Localise in the vascular endothelial cells and multiply to cause thrombosis lead to rupture & necrosis
Lab diagnosis of Sexually transmitted Infections (STIs)Mostafa Mahmoud
This lecture was presented to the physicians dealing with the various infectious diseases specially in STIs in Riyadh Region, MOH. The lecture concentrates about the various methodology applied to diagnose STIs in the laboratory with the advantages and disadvantages of each. Hope to make benefits to all.
lecture for MBBS students
Rickettsia named after HOWARD
TAYLOR RICKETTS died of Typhus fever contracted during his studies
Discovered spotted fever rickettsia (1906)
Obligate intracellular parasite
Gram negative pleomorphic rods
Parasite of arthropods – fleas, lice, ticks and mites.
No Human to human transmission.
Rickettsia are transmitted to humans by the bite of infected arthropod vector.
Multiply at the site of entry and enter the blood stream.
Localise in the vascular endothelial cells and multiply to cause thrombosis lead to rupture & necrosis
This presentation was made for class 11 & 12 students & was explained in detail during the seminar (SCIEN-CON’ 19).
This approach was taken by the medical students of MIDNAPORE MEDICAL COLLEGE, WEST BENGAL, INDIA for creating awareness about the health & hygiene and self assessment, knowledge & basic management of the most prevalent disease “Dengue”.
This was guided by the our beloved principal sir Dr. Panchanan Kundu & professors of other depts.
The school students (300) were divided in 6 grps & each were subdivided into 5 subgroups before grand lecture & were shown & demonstrated 6 major departments under guidance of medical students.
Seminar was attended by respective schools’ teachers.
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
This presentation was made for class 11 & 12 students & was explained in detail during the seminar (SCIEN-CON’ 19).
This approach was taken by the medical students of MIDNAPORE MEDICAL COLLEGE, WEST BENGAL, INDIA for creating awareness about the health & hygiene and self assessment, knowledge & basic management of the most prevalent disease “Dengue”.
This was guided by the our beloved principal sir Dr. Panchanan Kundu & professors of other depts.
The school students (300) were divided in 6 grps & each were subdivided into 5 subgroups before grand lecture & were shown & demonstrated 6 major departments under guidance of medical students.
Seminar was attended by respective schools’ teachers.
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
For over 10 decades, agents of infectious diseases have been identified through their phenotype directly in specimen and after a growth in culture.
Today, we are in a molecular era, there is an opportunity to detect organisms more rapidly and accurately based on their genetic signatures.
Biomedical science research discovery offers a growing numbers of a nucleic acid amplification tests (NAATS) among which is polymerase chain reaction (PCR) for detection and identification of bacterial, parasitic, fungi and viral pathogens.
These assays improve patient care, reduce antibiotic usage, enhance test utilization and increase laboratory and hospital efficiency.
In this seminar, we will explore the clinical usefulness and potential of both conventional and real-time PCR assays in Clinical Microbiology.
Pyrexia of unknown origin (PUO) may be defined as any febrile illness (temperature greater than 38°C) lasting 3 weeks or longer, without any obvious cause and failure to reach a diagnosis despite one week of inpatient investigation.
In these conditions there is thus a special need for a lab diagnosis
to guide the choice of
appropriate therapy.
Fever ≥ 38.3°C (>101°F) on several occasions
Virus is an obligate intracellular parasite which infects human beings, lower animals, insects, plants, bacteria and fungus. Viruses of medical importance to humans comprise of seven families of DNA viruses and 14 families of RNA viruses. Laboratory diagnosis of viral infections is continuously being refined to accelerate the process of identification of viruses. Because of the expense & the delay involved in obtaining a definitive virological diagnosis, discrimination in their use has to be done
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...
Meningitits
1.
2. CONENETS
• Introduction
• Definition
• Etiology
• Modes of spread
• Characteristic nature of organism
• Identification test for organism
• Pathogenesis
• Symptoms
• Prophylactic treatment
• Treatment, Diagnostic tests, Chemotherapy
3. Introduction
• Meningitis is always cerebrospinal infection. It is a rare infection that affects
delicate membranes that covers brain and spinal cord.
• Several types of disease, including bacterial, fungi, viral
• Bacterial meningitis can be life- threatening and
spreads between people in close contact with each
other
4. Definition
It is defined as an acute inflammation of the
pia mater and the arachnoid membrane
surrounding the brain and spinal cord
5. ETIOLOGY
• Bacterial
• Viral
• Parasitic
• Physical injury
• Cancer
• Certain drug ( NASIDS)
• Head injury
• Cerebral abscess
• Middle ear infection
6. Route of entry
• Medical procedure
• Along peripheral nerves
• Blood or lymphatic system
• Skull or back bone fractures
7. Mode of spread
• Direct contact
• Indirect contact
• Respiratory droplets from nose and throat of infected people
• Kissing/close contact
• Contact with bodily wastes
• Sharing food, drinks, towels
8. Charteristic features
• NAME : Neisseria meningitidis
• SYNONYM: Meningococci, meningococcaemia, meningococcal infection,
meningococcal meningitis
• FAMILY: Neisseriaceae
• CHARACTERISTICS:
• Gram – negative , Non- spore forming, Non- motile, encapsulated
organism.
• Non acid-fast diplococci, which appears in kidney bean shape under the
microscope.
9. characteristics
• Medium sized, smooth, transparent, non-pigmented, non-hemolytic, and
convex colonies are produced on blood agar after overnight incubation at
35-37degree C
• It requires an aerobic environment with 5% CO2 and enriched media
containing blood for growth
• It has at least 12 serogroups, with serogroups A, B, C, W-135, Y are being
the most commonly encountered serogroups from invasive disease cases
• It is oxidase and catalase positive
10. Identification test
• KOVAC’S OXIDASE TEST: this test determines the presence of
cytochrome oxidase.
• Here kovac’s oxidase reagent is used
• End point is purple color, if organism contains
cytochrome c as part of their respiratory chain
• This test aids in the recognition of N. Meningitides,
but other member of the genus Neisseria, as well as
unrelated bacterial species, may also give a positive reaction.
11. • Positive and negative quality control (qc) strains should be tested along with
the unknown isolates to ensure that the oxidase reagent is working properly.
CARBOHYDRATE UTILIZATION TEST (cystine trypticase agar
method)(CTA): This test are used to validate the identification of a strain as
N. meningitidis.
• Phenol red is used as indicator, that develops a yellow
color in the presence of acid at PH of 6.8 or less.
IDENTIFICATION OF N. MENINGITIDIS SEROGROUP:
• Twelve serogroups, based on the biochemical
composition of capsular polysaccharides are
currently recognized: A, B, C, H, I, K, L, W135, X, Y, Z, and 29E
12. • Serogroup d is no longer recognized as a serogroup
serogroups A, B, C, W135 and Y are the 5 most common causes of
bacterial meningitis
• COMMERCIAL IDENTIFICATION KITS: several commercial
identification systems that use biochemical or enzymatic substrates are
available for identification of Neisseria spp.
• SEROTYPING AND SEROSUBTYPING OF N.MENINGITIDI
WITH MONOCLONAL ANTIBODIES(Mabs)
15. • Fever and vomiting
• Sever headache
• Stiff neck
• Dislike of bright lights
• Very sleepy/difficult to wake
• Confused
• Seizures
• Cold hands or feet
• Mottled skin
• Positive kerning’s sign
• Positive Brudzinski’s sign
• Coma
• Rashes
• Photophobia
16. Prophylactic treatment
• Prophylaxis should be given to household members and kissing and saliva-
exchanging contacts of a case of meningococcal meningitis. The decision to
give prophylaxis to extended family contacts, close neighbour contacts or
children attending day-care center where a case has occurred is
controversial. It does not alter the course of an epidemic and close contacts
are liable to become reinfected soon after prophylaxis.
• Prophylaxis of H. influenzae should be given to households in which there
is at least one child under 48 months of age.
17. Epidemic focus
• The patient is hospitalized and hospitalized and isolated to condition
that results of two pharyngeal mucus are negative
• Contacts and carriers should be treated with rifampicin for 3days as a
prophylactic measures, the standard dose being given 3 times a day
• Polysaccharide meningococcal vaccines have been recently developed
in some countries.
18. treatment
• most patients are given I.V antibiotic until the laboratory finding determine the type of
meningitis. However, cultures should be taken before initiating antibiotics.
• To manage inflammation, dexamethasone (Decadron) or another corticosteroids is given
I. V.
• This steroids should be used before or with the first dose od antibiotics (I.V 0.6
mg/kg/day in four divided doses for first 4 days of antibiotics) and should be confined to
patients older than age 6weeks.
• Anti-fungal agent, such as amphotericin B (fungizone) and the triazoles, fluconazole
(Diflucan) and itraconazole (sporanox).
20. blood cultures
This is important when a meningococcal infection is suspected.
Fungal culture:
• Done on sabouraud’s dextrose agar or brain heart infusion agar.
• Two sets of medium was inoculated and incubated at 25˚C and37˚C,growth is
identified by its morphology
• Bird seed agar is used for culture of cryptococcus species.
Viral culture:
• Done using tissue culture, egg inoculation or animal
inoculation.
21. Imaging
Computerized tomography (CT) or magnetic resonance (MR) scan of
the head may show swelling or inflammation. X-RAY or CT scan of the
chest or sinuses may also show infection in other areas that may be
associated with meningitis.
22. Spinal tap (LUMBAR PUNTURE)
For a definitive diagnosis of meningitis, you’ll need a spinal tap to
collect cerebrospinal fluid (CSF). In people with meningitis, the CSF
often shows a low sugar (glucose) level along with an increased white
blood cell count and increased protein.
23. spinal tap
CSF analysis may also help your doctor identify which bacterium
caused the meningitis. If your doctor suspects viral meningitis. He or
she may order a DNA-based test known as a polymerase chain reaction
(PCR) amplification or a test to check for antibodies against certain
viruses to determine the determine the specific causes and determine
proper treatment.
24. Microscopical examination
• Microscopic examination of a gram-stained smear of CSF is done to
detect the presences of microorganism. if negative latex agglutination
test is done.
• ziehl-neelsen stained smear is performed for tubercle bacilli; if
negative PCR detection can be done.
• India ink preparation of CSF is done to demonstrate capsulated
budding yeast cell of cryptococcus neoformans
25. bacterial culture
• Chocolate and blood agar are used.
• The specimen is inoculated into an enriched liquid medium.
• Specimen may contain small bacteria which may not grow in solid culture
medium.
• After 24hours incubation of liquid medium,
subculture were made in solid culture media.
• If growth in culture, an antibiotic sensitivity
test is performed.
26. Serology
• Latex agglutination test for antigen detection: This done for s. pneumoniae, H.
influenzae and In, neonates ,test for antigens of group B streptococcus
• Serological test for antibodies to viruses: done depending on clinical diagnosis.
• Test to detect microbial antigen in urine: this test used to detect antigens present in
urine.
• Detection of microbial endotoxin: limulus lysate test is extremely sensitive for
detection of bacterial endotoxins.