The author has derived the formula to analytically compute all the important parameters of a disphenoid (isosceles tetrahedron with four congruent acute-triangular faces) such as volume, surface area, vertical height, radii of inscribed & circumscribed spheres, solid angle subtended at each vertex, coordinates of vertices, in-centre, circum-centre & centroid of a disphenoid for the optimal configuration in 3D space. The author has also proved the important conclusions related to a disphenoid by mathematical derivations using 3D coordinate geometry.