LIPOChip
•Download as PPT, PDF•
0 likes•993 views
The document describes LIPOchip, a DNA chip used to detect mutations related to Familial Hypercholesterolemia. It can detect over 240 mutations in the LDLR gene, 3 in the APOB gene, and 6 in the PCSK9 gene. It also detects copy number variations. The chip contains DNA fragments complementary to the target mutations. Samples are amplified, fragmented, labeled, and hybridized to the chip before being scanned and analyzed using specialized software.
Report
Share
Report
Share
Recommended
2011 course on Molecular Diagnostic Automation - Part 2 - Amplification
2011 course on Molecular Diagnostic Automation - Part 2 - Amplification.
This is from early 2011. Prices and Specifications of instruments may have changed.
Part 2 of 3
miScript Single Cell Poster
Single-cell microRNA expression profiling is a challenging workflow. From cell lysis, reverse transcription, preamplificatin to real-time PCR, every step involves technical pitfalls. Therefore it is critical to have a robust system that facilitates universal cDNA synthesis and universal amplification of all miRNAs in one workflow without introducing bias. Here we present a new poster – introducing a robust real-time PCR workflow and protocol for profiling miRNA expression from a single cell and how we analyze the single cells by using the free data analysis software.
Deeper Insight into Transcriptomes! Download the Flyer
Discover a new workflow for RT-PCR-based gene expression work
Accurate and biologically relevant results in RT-PCR-based
gene expression can be difficult to achieve. Successful
transcriptome work requires validated, reproducible targets
and high-quality technology. Recognizing the variability arising from sample physiology
and pathology, the influence of sample purification and
assay conditions, and the importance of access to easyto-
use software, QIAGEN experts developed a new gene
expression workflow. It will help you properly validate your
RT-PCR and gain the deepest insight into your result.
Technical Guide to Qiagen PCR Arrays - Download the Guide
Total RNA discovery with RT2 and miScript PCR Arrays : Explore the RNA universe - Whatever your destination within the RNA universe, QIAGEN will help you get there. The miRNeasy kits deliver pure, high-quality total RNA from a broad range of samples. The RT2 and miScript PCR arrays are a complete solution both for focused analysis of gene and microRNA expression and for validation of microarray and RNA sequencing experiments. Together with the powerful analytics tools of GeneGlobe® and QIAGEN Ingenuity® Pathway Analysis, these products give you a smooth path from your sample to high-quality results.
RT2 Profiler PCR Arrays: Pathway-focused Gene Expression Profiling with qRT-P...
This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT2 Profiler PCR Array System. The RT2 Profiler PCR Array System combines the quantitative performance of SYBR® Green real-time PCR with the multiple-gene profiling capabilities of a microarray.
The RT2 Profiler PCR Array is a 96- or 384-well plate containing RT2 qPCR Primer Assays for a set of 84 related genes, plus 5 housekeeping genes and 3 controls. The complete system includes an instrument-specific master mix and an optimized first strand synthesis kit. This paper presents experimental data showing that RT2 Profiler PCR Arrays have the sensitivity, reproducibility, and specificity expected from real-time PCR techniques. As a result, this technology brings focused gene expression profiling to any biological laboratory setting with a real-time PCR instrument.
Recommended
2011 course on Molecular Diagnostic Automation - Part 2 - Amplification
2011 course on Molecular Diagnostic Automation - Part 2 - Amplification.
This is from early 2011. Prices and Specifications of instruments may have changed.
Part 2 of 3
miScript Single Cell Poster
Single-cell microRNA expression profiling is a challenging workflow. From cell lysis, reverse transcription, preamplificatin to real-time PCR, every step involves technical pitfalls. Therefore it is critical to have a robust system that facilitates universal cDNA synthesis and universal amplification of all miRNAs in one workflow without introducing bias. Here we present a new poster – introducing a robust real-time PCR workflow and protocol for profiling miRNA expression from a single cell and how we analyze the single cells by using the free data analysis software.
Deeper Insight into Transcriptomes! Download the Flyer
Discover a new workflow for RT-PCR-based gene expression work
Accurate and biologically relevant results in RT-PCR-based
gene expression can be difficult to achieve. Successful
transcriptome work requires validated, reproducible targets
and high-quality technology. Recognizing the variability arising from sample physiology
and pathology, the influence of sample purification and
assay conditions, and the importance of access to easyto-
use software, QIAGEN experts developed a new gene
expression workflow. It will help you properly validate your
RT-PCR and gain the deepest insight into your result.
Technical Guide to Qiagen PCR Arrays - Download the Guide
Total RNA discovery with RT2 and miScript PCR Arrays : Explore the RNA universe - Whatever your destination within the RNA universe, QIAGEN will help you get there. The miRNeasy kits deliver pure, high-quality total RNA from a broad range of samples. The RT2 and miScript PCR arrays are a complete solution both for focused analysis of gene and microRNA expression and for validation of microarray and RNA sequencing experiments. Together with the powerful analytics tools of GeneGlobe® and QIAGEN Ingenuity® Pathway Analysis, these products give you a smooth path from your sample to high-quality results.
RT2 Profiler PCR Arrays: Pathway-focused Gene Expression Profiling with qRT-P...
This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT2 Profiler PCR Array System. The RT2 Profiler PCR Array System combines the quantitative performance of SYBR® Green real-time PCR with the multiple-gene profiling capabilities of a microarray.
The RT2 Profiler PCR Array is a 96- or 384-well plate containing RT2 qPCR Primer Assays for a set of 84 related genes, plus 5 housekeeping genes and 3 controls. The complete system includes an instrument-specific master mix and an optimized first strand synthesis kit. This paper presents experimental data showing that RT2 Profiler PCR Arrays have the sensitivity, reproducibility, and specificity expected from real-time PCR techniques. As a result, this technology brings focused gene expression profiling to any biological laboratory setting with a real-time PCR instrument.
Stable 16 year storage of DNA purified with the QIAamp® DNA Blood mini kit - ...
In this application note, we describe the success of the QIAamp DNA Blood Mini Kit in the preparation of highly stable DNA,as evidenced by 16-year storage data. We also report the best storage conditions for maximal protection against degradation.
Dna Methylation Analysis in a Single Day - Download the Slides
This webinar introduces the new PyroMark Q48 Autoprep system. Combined with the latest EpiTect Fast bisulfite conversion technology, the new PyroMark Q48 Autoprep can now provide highly automated methylation analysis in a single day.
New technology and workflow for integrated collection, stabilization and puri...
Research into non-invasive prenatal testing (NIPT) and circulating tumor DNA (ctDNA) testing based on circulating cell-free DNA (ccfDNA) is rapidly expanding. However, detection and quantification of ccfDNA is compromised by the release of genomic DNA (gDNA) from lymphocytes due to mechanical lysis or apoptosis during blood collection, storage and transport. PreAnalytiX has developed the PAXgene Blood ccfDNA System, consisting of the PAXgene Blood ccfDNA Tube, a plastic blood collection tube with a unique, non-crosslinking chemistry that preserves extracellular levels of ccfDNA and prevents the release of intracellular DNA from cells into the plasma, and the QIAsymphonyPAXgene Blood ccfDNA Kit for automated ccfDNA extraction from up to 5 ml of plasma. In this slidedeck, this new technology development is presented in comparison to other existing technologies.
Planning and Executing siRNA Experiments—Good Practices for Optimal Results
Planning and Executing siRNA Experiments—Good Practices for Optimal ResultsIntegrated DNA Technologies
Functional analysis by mRNA knockdown using siRNAs is now routine in many molecular biology labs. However, many RNAi experiments fail due to diversion from simple, good practices. This webinar will review the steps leading to successful siRNA experiments, including:
- Understanding the target transcript
- siRNA selection
- Choosing the cell type
- Validating the assay
- Including appropriate biological controlsAutomated Nucleic Acid Purification from Diverse Sample types using dedicated...
This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome. We will show how automation on the QIAcube enables efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, you will learn how to successfully use the CLC Microbial Genomics Module for metagenome sequencing and identification of microbial composition and diversity.
Extending miRQC’s dynamic range: amplifying the view of Limiting RNA samples ...
The original microRNA quality control (miRQC) study provided an in-depth analysis of commercially available microRNA (miRNA) quantification platforms. Specifically, twelve different
microarray, real-time PCR and small RNA sequencing platforms were assessed for reproducibility, sensitivity, accuracy, specificity and concordance of differential expression using a variety of sample types. Overall, each platform exhibited specific strengths and weaknesses, leading to the
final suggestion that a platform should be chosen on the basis of the experimental setting and the specific research questions. With this suggestion in mind, and the fact that liquid miRNA biopsies are an area of intense interest, we sought to expand the original miRQC study. For our “miRQC extension,” we benchmarked the QIAGEN miScript® PCR System with and without preamplification, and included a specific focus on routinely used biofluids. Concurrently, we benchmarked the miScript PCR System against another SYBR® Green miRNA detection platform. Overall, QIAGEN miScript demonstrated strong reproducibility and accuracy as well as superior detection rate and sensitivity in biofluids. Collectively, QIAGEN miScript provides the leading solution for novel miRNA discoveries.
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...Thermo Fisher Scientific
Survey and interview studies conducted over a three year period revealed that researchers are not satisfied with their current reverse transcriptase and are performing reactions with increasingly difficult samples, such as poorly purified RNA and unpurified RNA (direct RT) that both contain inhibitors. To meet this performance gap, the Thermo Fisher Life Sciences Solutions group produced a new reverse transcriptase, SuperScript® IV, and experiments we performed show that it is the most robust reverse transcriptase compared to other enzymes. SuperScript® IV characterization was performed in the context of “real world” situations where users do not have perfect RNA samples. In the presence of a variety of inhibitors, we demonstrate that SuperScript® IV possesses superior performance in a variety of inhibitors, such as alcohols, salts, detergents, phenol, heparin, hematin, bile salts, and formalin typically found in sample preparation reagents, cell lines, blood, feces, and FFPE samples. This enzyme can even detect RNA targets in unpurified RNA samples (directly lysed cells) and whole blood without sacrificing sensitivity and yield. The introduction of SuperScript® IV enables researchers to obtain more consistent results independent of sample quality and simplify and speed up workflows by eliminating RNA purification.Purification of total RNA from peripheral blood mononuclear cells - Download ...
Peripheral blood is often used for in vitro studies of the human immune system or immune responses, such as inflammation. An important part of the human immune system is represented by the peripheral blood mononuclear cells (PBMC). PBMC are blood cells characterized by a round nucleus and consist mainly of lymphocytes (T cells, B cells, and NK cells), macrophages and dendritic cells. Here, we describe the analysis of lipopolysaccharide-induced transcriptional response of isolated PBMC from whole blood using the RNeasy® Mini Kit or RNeasy Micro Kit, RT2 First Strand Kit, RT2 SYBR® Green ROX™ qPCR Mastermix, and RT2 Profiler PCR Arrays.
New Progress in Pyrosequencing for Automated Quantitative Analysis of Bi- or ...
Pyrosequencing is a highly flexible technology based on sequencing-by-synthesis for the rapid and quantitative analysis of any type of sequence variation. The real-time output delivers high-resolution sequence information, making pyrosequencing highly suitable for applications ranging from biallelic or multiallelic SNP analysis, DNA methylation quantification to complex mutation analysis of multiple sequence variations in a single run.
In this slideeck, we introduce the new PyroMark Q48 Autoprep system which enables fully automated template preparation integrated in the pyrosequencing workflow. In addition, a new Multiple Primer Dispensation (MPD) strategy is presented which allows fully automated dispensation of sequencing primer, offering a seamless workflow from samples to quantitative genotyping results.
This slidedeck focuses on the following topics
• Pyrosequencing technology and workflow in genotyping analysis
• Introduction into the new PyroMark Q48 Autoprep
• MPD strategy for a seamless automated pyrosequencing workflow
Join us and learn how you can apply the new pyrosequencing system and protocol to your variant analysis or genotyping research
Unveiling the Potential of your AAV Gene Therapy: Orthogonal methods to under...
Watch the presentation of this webinar here: https://bit.ly/3pCCjPF
Ensure your Adeno-Associated Virus (AAV) is safe throughout its entire drug development journey. Learn methods that will help you speed to clinic, potentially treating diseases sooner and with greater effectiveness.
The potential of gene therapies to cure previously untreatable diseases has spurred the development of novel drugs, including those based on Adeno-Associated Virus (AAV). As with all biopharmaceuticals, it is important to identify and monitor the critical quality attributes (CQAs) of these products to ensure their safety and efficacy.
In this webinar, we will present a range of orthogonal methods to understand and define the CQAs of AAV products. These include assays for the confirmation of capsid protein identity and quantity, as well as the characterization of important product-related impurities, such as aggregates. Together these methods represent a comprehensive analytical testing package to support the characterization and lot release of AAV products.
In this webinar, you will learn:
• How to identify and monitor the critical quality attributes (CQAs) of your AAV therapy
• What assays to utilize to confirm capsid protein identity and quantity
• Why you need look to product characterization to identify and remove important product-related impurities
Investigate the Molecular Basis of Disease
Investigate the molecular basis of complex disease by using Qiagen Bioinformatics solution and Pyrosequencing methods.
Improved Platforms for CHO Cell Line Development
Review of the integration of CHOZN and UCOE cell line development platforms, and the performance of the combined systems.
Streamlined next generation sequencing assay development using a highly multi...
Streamlined next generation sequencing assay development using a highly multi...Thermo Fisher Scientific
Next generation sequencing (NGS) assay development for solid tumor sequencing requires characterization of variant calling directly from formalin-fixed paraffin embedded (FFPE) tissue samples. However, cell line based FFPE and human FFPE samples only contain 2 to 20 variants, which require laboratories to invest significant resources in sample sourcing and preparation when developing assays to detect 100+ variantsComparison of Different NGS Library Construction Methods for Single-Cell Sequ...
Recent advances in whole genome amplification (WGA), whole transcriptome amplification (WTA) technologies and next-generation sequencing (NGS) have enabled whole genome or transcriptome sequencing at the single-cell level. Single-cell sequencing studies have yielded new insights into the heterogeneity of the genome and transcriptome in individual cells. Such heterogeneity at the single-cell level has been shown to be closely related to cellular function, differentiation, development, and diseases. A critical element of the single-cell sequencing workflow is sequencing library construction following WGA or WTA. An efficient library construction method is required to convert a high percentage of the DNA fragments to an adaptor-ligated sequencing library and to ensure high sequence complexity of the library. Furthermore, uniform representation of all genomic regions in a sequencing library is essential for retaining all important sequence information.
Here we compared 2 library construction methods following a REPLI-g MDA-mediated WGA or WTA:
• A ligation-based library construction method using a GeneRead Library Prep Kit (QIAGEN)
• A ‘tagmentation’-based method using Nextera DNA Sample Prep Kit (Illumina), which simultaneously fragments and tags DNA.
Our results demonstrated that the Nextera library construction method can be directly used with the REPLI-g-amplified DNA following MDA reaction, without the need for DNA purification. This could be beneficial if working with a high number of samples or if the complete workflow of single WGA/WTA and library construction should be automated. However, compared with the tagmentation method, the ligation-based library construction method is more flexible with regard to the input DNA amount and delivers sequencing libraries with higher complexity and less bias. This is critical for sensitive applications, such as identification of genomic variants or comprehensive profiling of transcriptomes.
More Related Content
What's hot
Stable 16 year storage of DNA purified with the QIAamp® DNA Blood mini kit - ...
In this application note, we describe the success of the QIAamp DNA Blood Mini Kit in the preparation of highly stable DNA,as evidenced by 16-year storage data. We also report the best storage conditions for maximal protection against degradation.
Dna Methylation Analysis in a Single Day - Download the Slides
This webinar introduces the new PyroMark Q48 Autoprep system. Combined with the latest EpiTect Fast bisulfite conversion technology, the new PyroMark Q48 Autoprep can now provide highly automated methylation analysis in a single day.
New technology and workflow for integrated collection, stabilization and puri...
Research into non-invasive prenatal testing (NIPT) and circulating tumor DNA (ctDNA) testing based on circulating cell-free DNA (ccfDNA) is rapidly expanding. However, detection and quantification of ccfDNA is compromised by the release of genomic DNA (gDNA) from lymphocytes due to mechanical lysis or apoptosis during blood collection, storage and transport. PreAnalytiX has developed the PAXgene Blood ccfDNA System, consisting of the PAXgene Blood ccfDNA Tube, a plastic blood collection tube with a unique, non-crosslinking chemistry that preserves extracellular levels of ccfDNA and prevents the release of intracellular DNA from cells into the plasma, and the QIAsymphonyPAXgene Blood ccfDNA Kit for automated ccfDNA extraction from up to 5 ml of plasma. In this slidedeck, this new technology development is presented in comparison to other existing technologies.
Planning and Executing siRNA Experiments—Good Practices for Optimal Results
Planning and Executing siRNA Experiments—Good Practices for Optimal ResultsIntegrated DNA Technologies
Functional analysis by mRNA knockdown using siRNAs is now routine in many molecular biology labs. However, many RNAi experiments fail due to diversion from simple, good practices. This webinar will review the steps leading to successful siRNA experiments, including:
- Understanding the target transcript
- siRNA selection
- Choosing the cell type
- Validating the assay
- Including appropriate biological controlsAutomated Nucleic Acid Purification from Diverse Sample types using dedicated...
This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome. We will show how automation on the QIAcube enables efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, you will learn how to successfully use the CLC Microbial Genomics Module for metagenome sequencing and identification of microbial composition and diversity.
Extending miRQC’s dynamic range: amplifying the view of Limiting RNA samples ...
The original microRNA quality control (miRQC) study provided an in-depth analysis of commercially available microRNA (miRNA) quantification platforms. Specifically, twelve different
microarray, real-time PCR and small RNA sequencing platforms were assessed for reproducibility, sensitivity, accuracy, specificity and concordance of differential expression using a variety of sample types. Overall, each platform exhibited specific strengths and weaknesses, leading to the
final suggestion that a platform should be chosen on the basis of the experimental setting and the specific research questions. With this suggestion in mind, and the fact that liquid miRNA biopsies are an area of intense interest, we sought to expand the original miRQC study. For our “miRQC extension,” we benchmarked the QIAGEN miScript® PCR System with and without preamplification, and included a specific focus on routinely used biofluids. Concurrently, we benchmarked the miScript PCR System against another SYBR® Green miRNA detection platform. Overall, QIAGEN miScript demonstrated strong reproducibility and accuracy as well as superior detection rate and sensitivity in biofluids. Collectively, QIAGEN miScript provides the leading solution for novel miRNA discoveries.
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...Thermo Fisher Scientific
Survey and interview studies conducted over a three year period revealed that researchers are not satisfied with their current reverse transcriptase and are performing reactions with increasingly difficult samples, such as poorly purified RNA and unpurified RNA (direct RT) that both contain inhibitors. To meet this performance gap, the Thermo Fisher Life Sciences Solutions group produced a new reverse transcriptase, SuperScript® IV, and experiments we performed show that it is the most robust reverse transcriptase compared to other enzymes. SuperScript® IV characterization was performed in the context of “real world” situations where users do not have perfect RNA samples. In the presence of a variety of inhibitors, we demonstrate that SuperScript® IV possesses superior performance in a variety of inhibitors, such as alcohols, salts, detergents, phenol, heparin, hematin, bile salts, and formalin typically found in sample preparation reagents, cell lines, blood, feces, and FFPE samples. This enzyme can even detect RNA targets in unpurified RNA samples (directly lysed cells) and whole blood without sacrificing sensitivity and yield. The introduction of SuperScript® IV enables researchers to obtain more consistent results independent of sample quality and simplify and speed up workflows by eliminating RNA purification.Purification of total RNA from peripheral blood mononuclear cells - Download ...
Peripheral blood is often used for in vitro studies of the human immune system or immune responses, such as inflammation. An important part of the human immune system is represented by the peripheral blood mononuclear cells (PBMC). PBMC are blood cells characterized by a round nucleus and consist mainly of lymphocytes (T cells, B cells, and NK cells), macrophages and dendritic cells. Here, we describe the analysis of lipopolysaccharide-induced transcriptional response of isolated PBMC from whole blood using the RNeasy® Mini Kit or RNeasy Micro Kit, RT2 First Strand Kit, RT2 SYBR® Green ROX™ qPCR Mastermix, and RT2 Profiler PCR Arrays.
New Progress in Pyrosequencing for Automated Quantitative Analysis of Bi- or ...
Pyrosequencing is a highly flexible technology based on sequencing-by-synthesis for the rapid and quantitative analysis of any type of sequence variation. The real-time output delivers high-resolution sequence information, making pyrosequencing highly suitable for applications ranging from biallelic or multiallelic SNP analysis, DNA methylation quantification to complex mutation analysis of multiple sequence variations in a single run.
In this slideeck, we introduce the new PyroMark Q48 Autoprep system which enables fully automated template preparation integrated in the pyrosequencing workflow. In addition, a new Multiple Primer Dispensation (MPD) strategy is presented which allows fully automated dispensation of sequencing primer, offering a seamless workflow from samples to quantitative genotyping results.
This slidedeck focuses on the following topics
• Pyrosequencing technology and workflow in genotyping analysis
• Introduction into the new PyroMark Q48 Autoprep
• MPD strategy for a seamless automated pyrosequencing workflow
Join us and learn how you can apply the new pyrosequencing system and protocol to your variant analysis or genotyping research
Unveiling the Potential of your AAV Gene Therapy: Orthogonal methods to under...
Watch the presentation of this webinar here: https://bit.ly/3pCCjPF
Ensure your Adeno-Associated Virus (AAV) is safe throughout its entire drug development journey. Learn methods that will help you speed to clinic, potentially treating diseases sooner and with greater effectiveness.
The potential of gene therapies to cure previously untreatable diseases has spurred the development of novel drugs, including those based on Adeno-Associated Virus (AAV). As with all biopharmaceuticals, it is important to identify and monitor the critical quality attributes (CQAs) of these products to ensure their safety and efficacy.
In this webinar, we will present a range of orthogonal methods to understand and define the CQAs of AAV products. These include assays for the confirmation of capsid protein identity and quantity, as well as the characterization of important product-related impurities, such as aggregates. Together these methods represent a comprehensive analytical testing package to support the characterization and lot release of AAV products.
In this webinar, you will learn:
• How to identify and monitor the critical quality attributes (CQAs) of your AAV therapy
• What assays to utilize to confirm capsid protein identity and quantity
• Why you need look to product characterization to identify and remove important product-related impurities
Investigate the Molecular Basis of Disease
Investigate the molecular basis of complex disease by using Qiagen Bioinformatics solution and Pyrosequencing methods.
Improved Platforms for CHO Cell Line Development
Review of the integration of CHOZN and UCOE cell line development platforms, and the performance of the combined systems.
Streamlined next generation sequencing assay development using a highly multi...
Streamlined next generation sequencing assay development using a highly multi...Thermo Fisher Scientific
Next generation sequencing (NGS) assay development for solid tumor sequencing requires characterization of variant calling directly from formalin-fixed paraffin embedded (FFPE) tissue samples. However, cell line based FFPE and human FFPE samples only contain 2 to 20 variants, which require laboratories to invest significant resources in sample sourcing and preparation when developing assays to detect 100+ variantsComparison of Different NGS Library Construction Methods for Single-Cell Sequ...
Recent advances in whole genome amplification (WGA), whole transcriptome amplification (WTA) technologies and next-generation sequencing (NGS) have enabled whole genome or transcriptome sequencing at the single-cell level. Single-cell sequencing studies have yielded new insights into the heterogeneity of the genome and transcriptome in individual cells. Such heterogeneity at the single-cell level has been shown to be closely related to cellular function, differentiation, development, and diseases. A critical element of the single-cell sequencing workflow is sequencing library construction following WGA or WTA. An efficient library construction method is required to convert a high percentage of the DNA fragments to an adaptor-ligated sequencing library and to ensure high sequence complexity of the library. Furthermore, uniform representation of all genomic regions in a sequencing library is essential for retaining all important sequence information.
Here we compared 2 library construction methods following a REPLI-g MDA-mediated WGA or WTA:
• A ligation-based library construction method using a GeneRead Library Prep Kit (QIAGEN)
• A ‘tagmentation’-based method using Nextera DNA Sample Prep Kit (Illumina), which simultaneously fragments and tags DNA.
Our results demonstrated that the Nextera library construction method can be directly used with the REPLI-g-amplified DNA following MDA reaction, without the need for DNA purification. This could be beneficial if working with a high number of samples or if the complete workflow of single WGA/WTA and library construction should be automated. However, compared with the tagmentation method, the ligation-based library construction method is more flexible with regard to the input DNA amount and delivers sequencing libraries with higher complexity and less bias. This is critical for sensitive applications, such as identification of genomic variants or comprehensive profiling of transcriptomes.
What's hot (20)
Stable 16 year storage of DNA purified with the QIAamp® DNA Blood mini kit - ...
Stable 16 year storage of DNA purified with the QIAamp® DNA Blood mini kit - ...
Dna Methylation Analysis in a Single Day - Download the Slides
Dna Methylation Analysis in a Single Day - Download the Slides
New technology and workflow for integrated collection, stabilization and puri...
New technology and workflow for integrated collection, stabilization and puri...
Planning and Executing siRNA Experiments—Good Practices for Optimal Results
Planning and Executing siRNA Experiments—Good Practices for Optimal Results
Automated Nucleic Acid Purification from Diverse Sample types using dedicated...
Automated Nucleic Acid Purification from Diverse Sample types using dedicated...
Extending miRQC’s dynamic range: amplifying the view of Limiting RNA samples ...
Extending miRQC’s dynamic range: amplifying the view of Limiting RNA samples ...
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...
Purification of total RNA from peripheral blood mononuclear cells - Download ...
Purification of total RNA from peripheral blood mononuclear cells - Download ...
Neil leblanc f7 rapid methods call amsterdam may 2010
Neil leblanc f7 rapid methods call amsterdam may 2010
New Progress in Pyrosequencing for Automated Quantitative Analysis of Bi- or ...
New Progress in Pyrosequencing for Automated Quantitative Analysis of Bi- or ...
Unveiling the Potential of your AAV Gene Therapy: Orthogonal methods to under...
Unveiling the Potential of your AAV Gene Therapy: Orthogonal methods to under...
PRODUCTION OF SEROTYPE 6-DERIVED RECOMBINANT ADENO-ASSOCIATED VIRUS IN SERUM-...
PRODUCTION OF SEROTYPE 6-DERIVED RECOMBINANT ADENO-ASSOCIATED VIRUS IN SERUM-...
Streamlined next generation sequencing assay development using a highly multi...
Streamlined next generation sequencing assay development using a highly multi...
Comparison of Different NGS Library Construction Methods for Single-Cell Sequ...
Comparison of Different NGS Library Construction Methods for Single-Cell Sequ...
Viewers also liked
Halifax Index 2012 Presentation
The Halifax Index tells Halifax, Nova Scotia's story - the strength of the economy, the health of the community, the sustainability of the environment and the progress of the Economic Strategy. It’s a new and smarter way to measure progress that reaches beyond traditional economic indicators like GDP and jobs. It provides deeper insight and the most comprehensive look at what’s happening in Halifax than anything currently available, and could be used as a model for other Canadian cities.
The 1st Halifax Index was presented at the Halifax State of the Economy Conference www.agreaterhalifax.com
Energy-Price-Driven Query Processing in Multi-center Web Search Engines
Concurrently processing thousands of web queries, each with a response time under a fraction of a second, necessitates maintaining and operating massive data centers. For large-scale web search engines, this translates into high energy consumption and a huge electric bill. This work takes the challenge to reduce the electric bill of commercial web search engines operating on data centers that are geographically far apart. Based on the observation that energy prices and query workloads show high spatio-temporal variation, we propose a technique that dynamically shifts the query workload of a search engine between its data centers to reduce the electric bill. Experiments on real-life query workloads obtained from a commercial search engine show that significant financial savings can be achieved by this technique.
Networking 101 - Building Relationships
Networking 101 tips as part of our Connector Program. For more information, visit halifaxpartnership.com
Delivery Of Future Content
BT and strategic research into next-generation communication and applications.
Presented at "Implementing Future Networks, Content and Services with Secure and Efficient Systems." At the University of Surrey 20th Sept 2010
Diabetes For Dummies, 3rd Edition by Alan L. Rubin, MD Index
Diabetes For Dummies, 3rd Edition by Alan L. Rubin MD Index
http://www.drrubin.com
Smart business Annual Report 2011
From April 1st, 2011 to March 31st 2012, the Partnership's SmartBusiness team consulted with 251 businesses in Halifax, NS, the majority of which were Small and Medium sized Enterprises (SMEs). This report is a compilation of the 213 retention visits to local businesses and their experiences with the Halifax economy. Retention visits cover a variety of issues ranging from: perceptions of the local business climate, to the company’s local workforce, to sales, even immigration issues.
Viewers also liked (20)
Energy-Price-Driven Query Processing in Multi-center Web Search Engines
Energy-Price-Driven Query Processing in Multi-center Web Search Engines
PA EI Leadership Meeting: Technology to Suppoty EI Leaders
PA EI Leadership Meeting: Technology to Suppoty EI Leaders
Diabetes For Dummies, 3rd Edition by Alan L. Rubin, MD Index
Diabetes For Dummies, 3rd Edition by Alan L. Rubin, MD Index
Similar to LIPOChip
Multiplex Assays for Studying Gene Regulation and Cell Function
Multiplex Assays for Studying Gene Regulation and Cell FunctionMiraiBio Group of Hitachi Solutions America, Ltd.
Please note: This presentation accompanies a recorded webinar at:
https://www1.gotomeeting.com/register/347794241
Biomarkers for studying gene regulation and cell function can be efficiently analyzed by multiplexed methods. Dr. Jim Lazar from OriGene Technologies will provide an overview of four different but related detection technologies that can be used to analyze genetic variants, microRNA expression, transcription factor binding, and protein expression on the Luminex xMAP platform. OriGene’s broad panel of assays and tools for discovery, analysis and validation of multiple classes of important biomarkers will allow researcher to develop more accurate descriptions of biologically complex systems.Expanding Your Research Capabilities Using Targeted NGS
Target enrichment enables researchers to focus their next generation sequencing (NGS) efforts on regions of interest, allowing them to obtain more sequencing data relevant to their study. In-solution target capture is a method of enrichment using oligonucleotide probes directed to specific regions within a genome. Target capture can be used to enrich multiple samples simultaneously, reducing the cost per sample, while using individually synthesized probes allows researchers to construct gene panels that can be optimized over time.
Next Generation Diagnostics: Potential Clinical Applications of Illumina’sTec...
Rob Kelley, MBA
Global Sales Manager
Translational Genomics
September 27, 2011
Principle, Procedure and applications of Digital PCR.pptx
Digital PCR (dPCR) is the new generation PCR that enables absolute quantification of target gene by separating reac¬tion mixture in several compartments. In this system, copies of target nucleic acid are distributed randomly from 0, 1 or many in the several small volume compartments. Amplification is occurred in the compartment and resulting absorbance is measured. Integrated fluidic circuits, chip based microchambers, and water in oil droplets are the methods are used for separation of reaction mixture in to several compartments. BioMark HD system (Fluidigm, USA) and QuantStudio3D system (Thermofisher Scientific, USA) uses integrated fluidic circuits harbor¬ing 10000 to 40000 microchambers and integrated chip containing 20000 microchambers, respectively. In droplet digital PCR System (Biorad, USA), reaction mixture is separated into 20,000 water in droplets. After cycles of reaction using any of the above technologies, fluorescence is detected with an imaging sys¬tem in the each compartment and copy numbers of the target is calculated with imaging software.
In droplets digital PCR, after amplification, droplets containing target gene are detected by fluorescence and scored as positive, and droplets without fluorescence are scored as negative. Poisson statistical analysis of the numbers of positive and negative droplets yields absolute quantitation of the target sequence. Digital PCR is the preferred technique for absolute quantification of target gene without need of standard curve and higher sensitivity and produces highly reproducible results, and also less susceptible to inhibitors than conventional RT-qPCR
DNA Microarray introdution and application
A DNA microarray By Neeraj Sharma For Microbiology Immunology students Under Tribhuwan University
Molecular Methods for Diagnosis of Genetic Diseases
Basic steps in the evolution of biotechnologic methods in Diagnosis of Genetic diseases
Targeted RNAseq for Gene Expression Using Unique Molecular Indexes (UMIs): In...
Traditional RNA sequencing (RNA-Seq) is a powerful tool for expression profiling, but is hindered by PCR amplification bias and inaccuracy at low expressing genes. QIAseq RNA is a flexible and precise tool developed for mitigating these complications, allowing digital gene expression analysis. This in-depth webinar will cover sample requirements, experimental design, NGS platform-specific challenges and workflow for gene enrichment, library prep and sequencing. The applications of QIASeq RNA Panels in cancer research, stem cell differentiation and elucidating the effects small molecules on signaling pathways will be highlighted.
Understanding Melt Curves for Improved SYBR® Green Assay Analysis and Trouble...
Understanding Melt Curves for Improved SYBR® Green Assay Analysis and Trouble...Integrated DNA Technologies
qPCR assays using intercalating dyes, such as SYBR® Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of melt curve analysis. In this presentation, Nick Downey, PhD, covers melt curve basics and shares examples of multiple peaks due to suboptimal sample prep, primer dimers, and asymmetric GC content of amplicons. He demonstrates troubleshooting strategies. Experienced and novice users will benefit from an overview of uMeltSM software, developed by the Wittwer lab at the University of Utah, that can predict the melt profile of your assay before you run your experiment.Similar to LIPOChip (20)
Multiplex Assays for Studying Gene Regulation and Cell Function
Multiplex Assays for Studying Gene Regulation and Cell Function
Expanding Your Research Capabilities Using Targeted NGS
Expanding Your Research Capabilities Using Targeted NGS
Next Generation Diagnostics: Potential Clinical Applications of Illumina’sTec...
Next Generation Diagnostics: Potential Clinical Applications of Illumina’sTec...
Principle, Procedure and applications of Digital PCR.pptx
Principle, Procedure and applications of Digital PCR.pptx
Molecular Methods for Diagnosis of Genetic Diseases
Molecular Methods for Diagnosis of Genetic Diseases
Targeted RNAseq for Gene Expression Using Unique Molecular Indexes (UMIs): In...
Targeted RNAseq for Gene Expression Using Unique Molecular Indexes (UMIs): In...
Understanding Melt Curves for Improved SYBR® Green Assay Analysis and Trouble...
Understanding Melt Curves for Improved SYBR® Green Assay Analysis and Trouble...
Recently uploaded
How to Give Better Lectures: Some Tips for Doctors
Talk given to Koronadal Internists Society, Marbel, South Cotabato as part of the Continuing Medical Education (CME) series.
The Normal Electrocardiogram - Part I of II
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Phone Us ❤85270-49040❤ #ℂall #gIRLS In Surat By Surat @ℂall @Girls Hotel With...
Phone Us ❤85270-49040❤ #ℂall #gIRLS In Surat By Surat @ℂall @Girls Hotel With 100% Satisfaction
KDIGO 2024 guidelines for diabetologists
KDIGO guidelines 2024 for evaluation and management of CKD, related to diabetes and management of diabetic kidney disease
Are There Any Natural Remedies To Treat Syphilis.pdf
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Physiology of Special Chemical Sensation of Taste
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Non-respiratory Functions of the Lungs.pdf
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Charaka Samhita Sutra sthana Chapter 15 Upakalpaniyaadhyaya
Charaka Samhita Sutra sthana Chapter 15 Upakalpaniyaadhyaya
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Flu Vaccine Alert in Bangalore Karnataka
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
263778731218 Abortion Clinic /Pills In Harare ,
263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journey
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Prix Galien International 2024 Forum Program
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Recently uploaded (20)
How to Give Better Lectures: Some Tips for Doctors
How to Give Better Lectures: Some Tips for Doctors
Pharynx and Clinical Correlations BY Dr.Rabia Inam Gandapore.pptx
Pharynx and Clinical Correlations BY Dr.Rabia Inam Gandapore.pptx
Phone Us ❤85270-49040❤ #ℂall #gIRLS In Surat By Surat @ℂall @Girls Hotel With...
Phone Us ❤85270-49040❤ #ℂall #gIRLS In Surat By Surat @ℂall @Girls Hotel With...
Triangles of Neck and Clinical Correlation by Dr. RIG.pptx
Triangles of Neck and Clinical Correlation by Dr. RIG.pptx
Are There Any Natural Remedies To Treat Syphilis.pdf
Are There Any Natural Remedies To Treat Syphilis.pdf
Charaka Samhita Sutra sthana Chapter 15 Upakalpaniyaadhyaya
Charaka Samhita Sutra sthana Chapter 15 Upakalpaniyaadhyaya
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journey
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journey
Maxilla, Mandible & Hyoid Bone & Clinical Correlations by Dr. RIG.pptx
Maxilla, Mandible & Hyoid Bone & Clinical Correlations by Dr. RIG.pptx
LIPOChip
- 2. The first DNA chip with CE MARK DETECTION OF THE MOST FREQUENT FH MUTATIONS AND COPY NUMBER CHANGES OF THE LDLR GENE
- 4. Biochip Chip/Array : DNA fragments (15-60bp) printed (spotted) on a solid support (glass) in an ordered way
- 5. Size of one spot : 50-100 μm 60-75mm Biochip
- 7. MOLECULAR BASIS OF HYBRIDIZATION A T C G T A G C AMPLIFICATION (PCR) ( region of interest) FRAGMENTATION DNAse: random cuts Alkaline Phosphatase : 3´ ends free 5´ 5´ 3´ 3´ LABELLING Biotin : Indirect labelling Patient DNA (COMPLEMENTARITY) + HYBRIDIZATION DNAchip With oligos specific to the mutations
- 8. N M Homozygous donor Heterozygous donor Technical base N N N M M M N N N
- 9. Experimental procedure DNA-chip design & printing Hybridization optimization SPECIFICATIONS Mutations/SNPs to be analyzed Image Capture and Software development GENOTYPE Oligonucleotide design Multiplex PCR design
- 12. Multiplex PCR Design M1 M2 M3 Exon 1 M4 M5 Exon 6
- 17. DAY1 DAY 2 OVERLAPPING PROCESSES 56 ul WORKFLOW 1 Amplification 7.5µl DNA (20ng/µl) 2 Fragmentation 3 Labelling 4 Hybridization 5 Results analysis 24 ul PCR mixes 1, 2 y 3 and 4 DNAse + Alkaline Phosphatase TdT + Biotin-ddUTP 2 hours 45 minutes 60 minutes 4 hours and 30 minutes
- 21. ESSENTIAL EQUIPMENT REQUIRED FOR PROCESSING 4. LIPOchip software: result analysis and report