Platelet Rich Plasma/PRP
Tendons and ligaments connect muscles to bones, making it possible to do all kinds of physical activities. Overuse or damage to tendons over a long period of time causes collagen fibers that make up the tendons to form small tears, a condition called tendinosis. Ligaments are composed of collagen fibers and hold bones together, stabilizing joints and controlling the range of motion. Tendons and ligaments have poor blood supply and they do not easily recover from damage caused by sprains, strains, and repetitive motion.
Anna Stahl President & CEO, LifeForm Healing Research, LLC
Platelet function and constituents of platelet rich plasma.Angad Malhotra
Int J Sports Med. 2013 Jan;34(1):74-80. doi: 10.1055/s-0032-1316319. Epub 2012 Aug 14.
Platelet function and constituents of platelet rich plasma.
Pelletier MH, Malhotra A, Brighton T, Walsh WR, Lindeman R.
SAGE Student Research Conference Poster- The Effect of Purified Acetaminophen...Melissa McCoy, MS, MBA
What is acetaminophen? Acetaminophen (APAP) is the active pharmaceutical ingredient of Tylenol® and other pharmaceutical generics, used as an analgesic. Previous experiments and data has suggested this molecule can potentially induce negative off-target effects in healthy, biological cells and tissues of the human body [1,2,3]. The specific effects discovered, of this small molecule included decreasing cell proliferative function, alter morphology, and omit intercellular protein interactions of normal cells [1,2,3]. If studies can biologically isolate the APAP’s function of causing these biological negative feedbacks, then experimental research on cancer cells should be eminent. It was originally hypothesized that the additive effects of Tylenol®, Advil®, and Aleve®, causes off-target effects on mouse lymphocytic leukemia cells (L1210) and over time, kill off the entire population. It was narrowed down to APAP, having the most extreme and quickest change in this cell’s proliferation and adhesion functions in a given time interval. Immortalized Human T Lymphocytes (Jurkat) were decided on because it needs to be seen if there is a biosimilar effect on a human cancer cell line. Therefore, it was hypothesized that APAP will suppress the Jurkat cell’s proliferative function, alter membrane shape, change the intercellular behavior, and induce apoptosis, due to the highly suggestive evidence that APAP signals to off-target proteins in biological cells. Knowing this information can potentially have researchers and biotech companies alike, further work with APAP and adjust it accordingly, as a potential oncotoxic molecule for cancer therapy.
Gamma-irradiated Frozen Cells Validated for GPCR-mediated Kinase SignalingPerkinElmer, Inc.
The use of frozen cells for cell-based screening has become widely accepted within the drug discovery community. Separating cell production from the actual screening campaign not only increases your flexibility but also improves the data consistency as the cellular material can be controlled and validated before running the functional assay.
Host Cell Protein Analysis by Mass Spectrometry | KBI BiopharmaKBI Biopharma
Host Cell Protein Analysis by Mass Spectrometry. Originally presented at the 2018 Sciex Users Meeting by Michael J Nold, Ph.D., Mass Spectrometry Core Facility at KBI Biopharma.
Platelet function and constituents of platelet rich plasma.Angad Malhotra
Int J Sports Med. 2013 Jan;34(1):74-80. doi: 10.1055/s-0032-1316319. Epub 2012 Aug 14.
Platelet function and constituents of platelet rich plasma.
Pelletier MH, Malhotra A, Brighton T, Walsh WR, Lindeman R.
SAGE Student Research Conference Poster- The Effect of Purified Acetaminophen...Melissa McCoy, MS, MBA
What is acetaminophen? Acetaminophen (APAP) is the active pharmaceutical ingredient of Tylenol® and other pharmaceutical generics, used as an analgesic. Previous experiments and data has suggested this molecule can potentially induce negative off-target effects in healthy, biological cells and tissues of the human body [1,2,3]. The specific effects discovered, of this small molecule included decreasing cell proliferative function, alter morphology, and omit intercellular protein interactions of normal cells [1,2,3]. If studies can biologically isolate the APAP’s function of causing these biological negative feedbacks, then experimental research on cancer cells should be eminent. It was originally hypothesized that the additive effects of Tylenol®, Advil®, and Aleve®, causes off-target effects on mouse lymphocytic leukemia cells (L1210) and over time, kill off the entire population. It was narrowed down to APAP, having the most extreme and quickest change in this cell’s proliferation and adhesion functions in a given time interval. Immortalized Human T Lymphocytes (Jurkat) were decided on because it needs to be seen if there is a biosimilar effect on a human cancer cell line. Therefore, it was hypothesized that APAP will suppress the Jurkat cell’s proliferative function, alter membrane shape, change the intercellular behavior, and induce apoptosis, due to the highly suggestive evidence that APAP signals to off-target proteins in biological cells. Knowing this information can potentially have researchers and biotech companies alike, further work with APAP and adjust it accordingly, as a potential oncotoxic molecule for cancer therapy.
Gamma-irradiated Frozen Cells Validated for GPCR-mediated Kinase SignalingPerkinElmer, Inc.
The use of frozen cells for cell-based screening has become widely accepted within the drug discovery community. Separating cell production from the actual screening campaign not only increases your flexibility but also improves the data consistency as the cellular material can be controlled and validated before running the functional assay.
Host Cell Protein Analysis by Mass Spectrometry | KBI BiopharmaKBI Biopharma
Host Cell Protein Analysis by Mass Spectrometry. Originally presented at the 2018 Sciex Users Meeting by Michael J Nold, Ph.D., Mass Spectrometry Core Facility at KBI Biopharma.
SEO Nashville: 25 Places to Earn Local Backlinks for your Business' WebsiteLSM
Local Search Masters provides a list of websites that are excellent places to obtain backlinks from. Using Nashville as the example city (though many of these tactics are applicable to cities outside of Nashville, TN), LSM shows screenshots of different, quality backlink examples from places like search engine profiles, local directories, industry-related associations and charitable organizations! For more information, please visit http://www.localsearchmasters.com/
Presentation on WSIS Implementation beyond 2015Jaroslaw Ponder
This presentation was delivered at the UN Commission for Science and Technology for Development Intersessional Panel on 13 January 2016 in Budapest, Hungary.
NOVEL IPQCL AND FPQC TEST FOR OPTHALMIC PREPARATION AS PER IP, BP A...roshan telrandhe
Ophthalmic preparation are the sterile liquid or semisolid preparation meant to installation in to the eyes in the space between eye lids and eye ball .
These product must be sterile and are prepare under the same condition as that of parenteral preparation
SEO Nashville: 25 Places to Earn Local Backlinks for your Business' WebsiteLSM
Local Search Masters provides a list of websites that are excellent places to obtain backlinks from. Using Nashville as the example city (though many of these tactics are applicable to cities outside of Nashville, TN), LSM shows screenshots of different, quality backlink examples from places like search engine profiles, local directories, industry-related associations and charitable organizations! For more information, please visit http://www.localsearchmasters.com/
Presentation on WSIS Implementation beyond 2015Jaroslaw Ponder
This presentation was delivered at the UN Commission for Science and Technology for Development Intersessional Panel on 13 January 2016 in Budapest, Hungary.
NOVEL IPQCL AND FPQC TEST FOR OPTHALMIC PREPARATION AS PER IP, BP A...roshan telrandhe
Ophthalmic preparation are the sterile liquid or semisolid preparation meant to installation in to the eyes in the space between eye lids and eye ball .
These product must be sterile and are prepare under the same condition as that of parenteral preparation
The PCT MicroTube Adapter Kit, in combination with the PCT SPS, can reliably and reproducibly control the enzymatic digestion of proteins while reducing the time of digestion from hours to minutes with the same or better quality as other, currently available techniques.
Find out more at : www.pressurebiosciences.com
pathogen inactivation of cellular components.pptxDrShinyKajal
Chemical inactivation
Photo-inactivation
Solvent-detergent Plasma
Photosensitizers
Methylene Blue light treatment
Psoralen Ultraviolet Light Treatment
Riboflavin Light Treatment
INTERCEPT System
Mirasol system
Platelet and plasma Pathogen Inactivation
FRALE and azridine compounds
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
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Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
3. 1. Pure PRP® is a unique blend of highly concentrated platelets and growth factors suspended in a bath
of plasma.
2. Pure PRP® is the only PRP composition that provides platelet concentrations of up to 9 times
baseline in 7mLs, with 99% of the RBCs REMOVED.
3. Pure PRP® can be processed with or without inflammatory neutrophil granulocytes. Protocol A
removes 99% of the granulocytes. Protocol B collects approximately 99% of the granulocytes. This
provides the clinician with a clinical option for the patient requirement.
4. Pure PRP® greatly enhances monocyte concentrations, up to 7 times baseline. Monocytes are the
largest of all leukocytes and are characteristically non-inflammatory phagocytic cells. They elicit an
immune response which last for longer periods of time (months rather than days when compared to
neutrophils).
5. BEST OF ALL - Pure PRP® is naturally pH balanced, averaging 7.4. It requires no buffering.
Basic Science
4. Reduced necrotic granulocytes reduces post injection inflammation in the joint space
Lower viscosity allows smaller injection needles. Higher fibrinogen levels provide a natural matrix
scaffold for platelet retention
The Effect of Platelet-Rich Plasma Formulations and Blood Products on Human Synoviocytes
Implications for Intra-articular Injury and Therapy
Hillary J. Braun,*y BA, Hyeon Joo Kim,* PhD, Constance R. Chu,* MD, and Jason L. Dragoo,*z MD
Investigation performed at Stanford University, Palo Alto, California, USA
Conclusion:
Treatment of synovial cells with PRP rich in RBCs resulted in significant cell death and pro-inflammatory
mediator production.
Clinical Relevance:
Clinicians should consider using, RBC-free formulations of PRP when administering intra-articularly.
Pure PRP® vs Conventional PRP
5. Comparison Data
Pure PRP® vs. SmartPrep® vs Angel
Study Date 5/5/2015
8 Patient Comparison Study
PurePRP® SmartPrep® Angel
Platelet Concentration 6.6x 5.66x 3.94x
Platelet Yield 80% 61% 45%
Total Deliverable Platelets 8,555,880,000 7,193,280,000 5,084,920,000
Granulocytes 2% 1% 3%
Monocytes 77% 27% 35%
Average PRP Volume 7.3 7.1 7.2
Pure PRP® vs SmartPrep® vs Angel
Principle Investigator(s): Dr. Robert Mandle, Ph.D.
Children’s Hospital, Harvard Medical School
The CBR Institute for Biomedical Research
Biosciences Research Associates, Boston, MA.
13. Preparation Technique
Protocol A
Aspirate PPS leaving the RBC behind
Protocol A processes PurePRP® without red
blood cells or neutrophil granulocytes. This
protocol is used when powerful healing without
inflammatory activity is required at the
application site. This protocol is also the low
viscosity solution to a viable PRP product,
providing very high concentrations of platelets
in a bath of non-viscous plasma. This protocol
has also been reported to reduce the potential
for pain at the application site. It is the most
frequently used protocol.
Protocol B
Aspirate PPS and an additional 1mL of RBC
Protocol B processes PurePRP® with low red
blood cell counts and very high neutrophil cell
recoveries. This protocol is used when the
phagocytic powers of neutrophils are needed to
help fight infectious processes at the application
site. This protocol produces the highest
chemoattractant activity and significantly
increases regeneration potential. Once the
neutrophils have completed phagocytosis, they
become apoptic cells and are subsequently
removed, thereby also eliminating the
inflammatory activity.
Platelet Plasma Suspension Preparation
21. The Secret to Improved Performance
The key to the 544E Concentrating System is the new
ClearVUE Conical Piston. The piston is specially designed
to greatly improve the collection of the cell concentrate
buffycoat layer. The primary feature of the new ClearVUE
Conical Piston is it's the deep conical shape. The deep
conical design perfectly directs the collection of the cell
concentrate buffycoat. This provides quality platelet rich
plasma and bone marrow concentrate with near perfect
collection yields. The ClearVUE Conical Piston also
features enhanced handling of the concentrating device
with frictionless motion and a CLEAR VIEW of the
buffycoat while its being aspirated, further instilling
confidence in the quality of the end product.
544E
THE NEW 5 MINUTE SOLUTION
Trapped
Plasma &
Cell Buffycoat
Collected
Plasma &
Cell Buffycoat
34. 1. PureBMC® is a unique blend of VIABLE concentrated platelets, hematopoetic stem cells,
mesenchymal stem cells and total nucleated cells in BMA plasma containing a hematocrit of less
than 25%.
2. PureBMC® provide HSC concentrations of up to 13 times the baseline and TNC concentrations of up
to 9 times the baseline, all in 7mL of PureBMC ® with a hematocrit of less than 25%
3. PureBMC® also has the ability to produce large quantities of colony forming units which is indicative
of it’s MSC growth propensity
4. PureBMC® is specially unique because it can be prepared with Citrate anticoagulant or with Heparin.
It’s been shown that either way preserves the platelet functionality and viability. This single feature
significantly improves the BMC effectiveness, because the platelet mediators and cytokines are at
optimal performance
5. BEST OF ALL - PureBMC® is naturally pH balanced with an average pH of 7.4. It requires no
buffering