- Shenzhen Leveking bio-engineering Co. Ltd produces phytase enzymes for use in animal feed to improve nutrient utilization.
- Phytate in feed binds phosphorus and other minerals, reducing their availability to animals. Leveking's phytase is added to feed to break down phytate, releasing nutrients and improving growth performance.
- A study found that adding Leveking phytase to broiler diets improved final weight, feed intake, and feed conversion ratio in a dose-dependent manner compared to controls.
This document summarizes a study on screening, optimizing, and producing phytase from Aspergillus species isolated from environmental samples. Key findings include:
1) Several Aspergillus isolates (A. niger, A. fumigatus, A. tamari) were able to produce high levels of phytase when grown in media containing organic substrates like orange peel, sesame, and watermelon seeds.
2) Culture conditions like pH, carbon and nitrogen sources, inoculum age, and substrates were optimized to improve phytase production. Maximum yields were obtained at pH 5.5 using glucose and (NH4)2SO4 and an inoculum age of 72 hours
The document discusses the role of enzymes in poultry nutrition. It notes that the poultry industry in India has seen 10-12% growth in broilers and 5-7% growth in layers. Enzyme feed additives can improve production performance by increasing nutrient utilization and preventing the effects of anti-nutritional factors like phytate. Phytate binds with minerals and other nutrients, reducing their availability, but phytase enzymes can break down phytate and release those bound nutrients. The addition of phytase supplements to poultry feed has been shown to improve growth performance, feed efficiency, egg production and nutrient utilization.
Nutrients found in feed materials are necessary for the maintenance, growth, production, and health of birds. Nutrients help break down food for energy, improve feed utilization, and enhance digestion, which improves growth and profitability. However, some plants also contain antinutrients like arabinoxylans, beta-glucans, cyclopropenoid fatty acids, gossypol, L-canavanine, phytate, protease inhibitors, saponins, and tannins, which must be considered when formulating feed. Feeds can be prepared locally from grains and feedstuffs, from local grains and imported concentrates, or as commercial feed.
This document discusses enzymes used in poultry and ruminant nutrition. It describes how enzymes are proteins that catalyze reactions without being consumed in the process. Exogenous enzymes from sources like bacteria, fungi and yeasts are added to animal feed to help break down nutrients. Specific enzymes discussed include beta-glucanases, xylanases, phytases, amylases and proteases. These enzymes help improve nutrient digestion and absorption, increase growth performance, and reduce nutrient excretion in waste. The document provides details on the chemical nature, modes of action and typical doses of various enzymes used in animal feed supplementation.
Phytase is an enzyme that helps monogastric animals like poultry and swine break down and absorb phosphorus from grains. It reduces the need for supplemental phosphorus in animal feed. Phytase increases phosphorus availability from phytate, the main form of phosphorus in plants. This allows less phosphorus to be supplemented to feed and excreted in manure. Phytase efficacy depends on factors like diet phytate level, source (fungal or bacterial), and processing conditions like temperature.
Value of Non-Phosphorus Effects of Exogenous Phytase in SwineRommel Sulabo
1. The document discusses studies examining the effects of supplemental phytase on protein and amino acid utilization in swine. Some studies found small improvements in protein and amino acid digestibility with phytase addition, while others found no effects.
2. The effects of phytase on nitrogen balance and retention in pigs are inconsistent, with one study finding no effects. Phytase effects may depend on dietary ingredients and phytate levels.
3. More research is needed to fully understand how phytase impacts protein digestion and amino acid availability in pigs, including growth performance studies and evaluations of total tract digestibility.
This document discusses proteases and their role in digestion in poultry. It begins by explaining that animals must obtain some amino acids from their diet as they lack the necessary enzymes to synthesize them. It then describes how ingested proteins are broken down through digestion involving acid and protease enzymes. The document provides classifications and examples of different types of proteases. It discusses how proteases break down proteins, fats, and carbohydrates at different pH levels along the gastrointestinal tract. The document also notes some anti-nutritional factors found in various feed ingredients and how proteases can help degrade them to improve digestibility.
The document discusses the role of enzymes in poultry nutrition. It describes how most poultry diets contain maize and soybeans which contain phytate and non-starch polysaccharides that can negatively impact nutrient absorption. The use of enzymes can help address this by breaking down these anti-nutrients. Specifically, exogenous enzymes are described that help supplement endogenous enzymes or aid in breaking down substances like beta-glucans and phytate that poultry cannot digest on their own. Research shows that enzymes can improve growth performance, nutrient utilization, and bone mineralization in poultry. Higher enzyme doses beyond 500 FTU/kg are also beneficial.
This document summarizes a study on screening, optimizing, and producing phytase from Aspergillus species isolated from environmental samples. Key findings include:
1) Several Aspergillus isolates (A. niger, A. fumigatus, A. tamari) were able to produce high levels of phytase when grown in media containing organic substrates like orange peel, sesame, and watermelon seeds.
2) Culture conditions like pH, carbon and nitrogen sources, inoculum age, and substrates were optimized to improve phytase production. Maximum yields were obtained at pH 5.5 using glucose and (NH4)2SO4 and an inoculum age of 72 hours
The document discusses the role of enzymes in poultry nutrition. It notes that the poultry industry in India has seen 10-12% growth in broilers and 5-7% growth in layers. Enzyme feed additives can improve production performance by increasing nutrient utilization and preventing the effects of anti-nutritional factors like phytate. Phytate binds with minerals and other nutrients, reducing their availability, but phytase enzymes can break down phytate and release those bound nutrients. The addition of phytase supplements to poultry feed has been shown to improve growth performance, feed efficiency, egg production and nutrient utilization.
Nutrients found in feed materials are necessary for the maintenance, growth, production, and health of birds. Nutrients help break down food for energy, improve feed utilization, and enhance digestion, which improves growth and profitability. However, some plants also contain antinutrients like arabinoxylans, beta-glucans, cyclopropenoid fatty acids, gossypol, L-canavanine, phytate, protease inhibitors, saponins, and tannins, which must be considered when formulating feed. Feeds can be prepared locally from grains and feedstuffs, from local grains and imported concentrates, or as commercial feed.
This document discusses enzymes used in poultry and ruminant nutrition. It describes how enzymes are proteins that catalyze reactions without being consumed in the process. Exogenous enzymes from sources like bacteria, fungi and yeasts are added to animal feed to help break down nutrients. Specific enzymes discussed include beta-glucanases, xylanases, phytases, amylases and proteases. These enzymes help improve nutrient digestion and absorption, increase growth performance, and reduce nutrient excretion in waste. The document provides details on the chemical nature, modes of action and typical doses of various enzymes used in animal feed supplementation.
Phytase is an enzyme that helps monogastric animals like poultry and swine break down and absorb phosphorus from grains. It reduces the need for supplemental phosphorus in animal feed. Phytase increases phosphorus availability from phytate, the main form of phosphorus in plants. This allows less phosphorus to be supplemented to feed and excreted in manure. Phytase efficacy depends on factors like diet phytate level, source (fungal or bacterial), and processing conditions like temperature.
Value of Non-Phosphorus Effects of Exogenous Phytase in SwineRommel Sulabo
1. The document discusses studies examining the effects of supplemental phytase on protein and amino acid utilization in swine. Some studies found small improvements in protein and amino acid digestibility with phytase addition, while others found no effects.
2. The effects of phytase on nitrogen balance and retention in pigs are inconsistent, with one study finding no effects. Phytase effects may depend on dietary ingredients and phytate levels.
3. More research is needed to fully understand how phytase impacts protein digestion and amino acid availability in pigs, including growth performance studies and evaluations of total tract digestibility.
This document discusses proteases and their role in digestion in poultry. It begins by explaining that animals must obtain some amino acids from their diet as they lack the necessary enzymes to synthesize them. It then describes how ingested proteins are broken down through digestion involving acid and protease enzymes. The document provides classifications and examples of different types of proteases. It discusses how proteases break down proteins, fats, and carbohydrates at different pH levels along the gastrointestinal tract. The document also notes some anti-nutritional factors found in various feed ingredients and how proteases can help degrade them to improve digestibility.
The document discusses the role of enzymes in poultry nutrition. It describes how most poultry diets contain maize and soybeans which contain phytate and non-starch polysaccharides that can negatively impact nutrient absorption. The use of enzymes can help address this by breaking down these anti-nutrients. Specifically, exogenous enzymes are described that help supplement endogenous enzymes or aid in breaking down substances like beta-glucans and phytate that poultry cannot digest on their own. Research shows that enzymes can improve growth performance, nutrient utilization, and bone mineralization in poultry. Higher enzyme doses beyond 500 FTU/kg are also beneficial.
This research evaluated the effect of a novel protease by DSM (RONOZYME® ProAct) on the performance, digestible energy and the ileal digestibility of crude protein, fat, gross energy and amino acids in broiler chickens. Read more to learn about the results.
Visit us at DSM Animal Nutrition and Health to learn more about our business: http://www.dsm.com/markets/anh/en_US/home.html
The document discusses optimizing protein digestion in poultry through the use of exogenous proteases, noting that both endogenous proteins secreted by the bird and undigested dietary proteins leave the ileum undigested. It examines factors that influence protein digestion like diet composition and quality, and endogenous protein sources and recovery. Models are presented that can help predict how diet inputs like crude protein level and amino acid ratios influence the efficacy of adding an exogenous protease supplement to improve protein digestion and bird performance.
This document discusses key factors to consider when selecting feed enzyme products for poultry production. It outlines three main classes of enzymes used in poultry feeds - phytases, carbohydrases, and proteases. When choosing an enzyme product, it is important to evaluate whether the enzyme will improve digestibility of the specific feed formulation, is thermostable for pelleting conditions, and is compatible with other enzymes. The document also stresses selecting enzymes that have proven mechanisms of action and are produced under quality manufacturing standards.
Knowledge of amino acid (AA) digestibility of feed ingredients is necessary to feed broilers with
properly balanced compound diets. For this reason, more attention has recently been given to the
determination of AA digestibility of ingredients, recognizing that it may vary greatly depending
upon the feed ingredient. The effects of the inclusion of a mono-component serine protease
(Ronozyme® ProAct) on standardized ileal amino acid digestibility (SIAAD) of diets containing wheat
by-products (wheat DDGS (WDDGS) and wheat middlings (WM)) were evaluated in broilers.
- Improvement of SIAAD in the presence of protease is not the same for all AA and varies from one ingredient to another one. The effects of added protease are dependent on feed composition and on intrinsic digestibility of AA (COWIESON and ROOS (2014)).
- Factors such as type and quality of the ingredients, industrial processing and the presence of anti-nutritional substances such as tannins, phytates, trypsin inhibitors in plant species modulate the digestibility in poultry feed and thus the effectiveness of exogenous protease.
-The physiological state of animals (growth or maintenance), feed consumption or the nutritional feed quality could also influence the digestibility values.
Animal feed accounts for 60-70% of livestock and poultry production costs. Supplementing feed with enzymes can help producers save costs by enabling animals to produce more meat faster and cheaper. There are several types of enzymes that play important roles in animal feed digestion. Proteases break down proteins, carbohydrases improve carbohydrate digestibility, and phytases allow animals to access phosphorus stored in plant feeds. Using enzymes can boost feed efficiency, lower feed costs, and reduce environmental impacts.
Enzyme technology is an integral tool for the brewing, baking and textile industries. In agriculture there is great potential for enzymes too but this is yet to be fully exploited. However, animal nutrition is one area in which the use of feed enzymes is becoming increasingly important.
This document discusses the use of exogenous enzymes in poultry nutrition as an alternative to antibiotics. It provides background on how antibiotics were commonly used but are now banned. Exogenous enzymes are one potential alternative as they can help break down non-starch polysaccharides in feed ingredients like wheat and barley to improve digestibility. The document outlines different types of enzymes and their sources and effects. It summarizes studies looking at how exogenous enzymes can reduce the size of digestive organs and positively impact gut morphology.
Phytate is a natural dietary content and constitutes 0.4–6.4% (w/w) of most cereals and legumes (Eeckhout and Deaepe, 1994). It is poorly digestible for monogastric animals due to
a lack of effective endogenous phytase (Bitar and Reinhold, 1972). Phytate acts as an antinutritional factor, exerting its effects via a reduction in the solubility, and availability of
phosphorus (P), and to a lesser extent, Ca, Zn, Fe (Nävert et al., 1985; Hallberg et al., 1987; Hurrell et al., 2003). It was also reported that phytate could decrease the utilization of protein,
amino acids and starch. It has been suggested that phytate may bind with starch either directly, via hydrogen bonds, or indirectly, via proteins associated with starch (Thompson,
1988; Rickard and Thompson, 1997). Phytate is also known to inhibit a number of digestive enzymes such as pepsin, alpha-amylase (Deshpande and Cheryan, 1984) and increase mucin
secretion, excretion of endogenous minerals and amino acids in broiler chickens (Liu et al., 2008). Another issue is higher cost of dietary inorganic P which has been increased remarkably in last decade because of shortened phospate sources. Poultry industry has still been growing and reached huge mass production and contribution to environmental pollution has been heightened concerns because of the poor utilization of phytate phosphorus by poultry.
This document summarizes research on the use of organic acids in animal nutrition. It discusses how organic acids have gained importance in feed industry following the ban of antibiotic growth promoters. Short chain organic acids can have antimicrobial properties. Research shows that organic acids can improve animal performance by reducing pathogenic bacteria counts, increasing nutrient digestibility, and improving gut health. Studies demonstrate that supplementing animal feed with organic acids like formic acid, fumaric acid, and butyric acid can positively impact growth performance, gut morphology, and nutrient utilization in poultry and pigs.
Phytase is an enzyme that helps non-ruminant animals like poultry and swine break down and absorb phosphorus from grains. It allows these animals to utilize phosphorus from phytate in grains, reducing the need for supplemental phosphorus in their diets. Phytase is produced by microbes like bacteria and fungi. It works by hydrolyzing phytate, making phosphorus more available to the animal. The use of phytase supplements in animal feed lowers feed costs and decreases phosphorus pollution by allowing animals to excrete less phosphorus.
This chapter provides an overview of the current feed enzyme market and expected future developments. The global feed enzyme market has grown substantially since the late 1980s as enzymes help improve feed efficiency and flexibility in feed formulation. Enzymes allow animals to extract more nutrients from feed by targeting anti-nutritional factors. While animals naturally produce some digestive enzymes, they cannot fully digest 15-25% of feed due to indigestible components. Feed enzymes help bridge this gap to improve animal performance and farm profitability by lowering feed costs. The market is expected to continue expanding with the use of enzyme combinations and customization to specific feed formulations.
A description and the results of research carried out on broiler chickens in order to explore the efficacy of phytase products on ileal digestibility of phosphorus.
The reseach results found that phytase supplementation was effective in improving the growth performance, ileal phosphorus digestibility and the bone mineralization parameters when included in the low phosphorus diet.
Visit us at: http://www.dsm.com/markets/anh/en_US/home.html
This study investigates the effect of Bacillus licheniformis protease on the growth performance and the nutrient utilization of broilers. This reduces dietary requirements and environmental impact of excretion whilst increasing energy utilisation and growth in broilers.
Join the LinkedIn discussion on Feed Enzymes:
http://www.linkedin.com/groups?home=&gid=4738175&trk=anet_ug_hm&goback=%25
Learn more about DSM Animal Nutrition at: http://www.dsm.com/markets/anh/en_US/home.html
Or why not follow us on Twitter: @DSMFeedTweet
Research has demonstrated that phytase is the only enzyme that is able to initiate
the release of phosphorus (P) from the phytate molecule, making it available for
absorption and utilization (Selle and Ravindran, 2007). The industrial demand for
phytases with greater potency in intestinal phytate hydrolysis and better heat
stability continues to stimulate the search for new enzyme sources. Enzyme
preparations with phytases derived from A. ficuum, Peniophora lycii and E. coli are
available commercially. More recently, new microbial 6-phytases produced by
synthetic genes, mimicking a gene from C. braakii or isolated from Buttiauxella,
were introduced into the market.
Protein/amino acids are among the most expensive nutrients to deliver in poultry nutrition
The digestibility of protein in poultry is typically incomplete by the terminal ileum
Undigested protein that leaves the ileum is from both exogenous (diet) and endogenous (bird) sources
Understanding the digestion of dietary proteins and the recovery of endogenous proteins is important and can provide a basis for the use of exogenous proteases
Presentation on Non starch polysaccharides in poultry final osrHarshit Saxena
Include knowledge of NSPs n important constituent in modern poultry farming . Mitigation and their utilization in non-conventional feeds remarkably increases profit
This study compared the efficacy of four phytase products - PHY1 from Citrobacter braakii, PHY2 from E.coli, PHY3 from E.coli, and PHY4 from Peniophora lycii - on growth performance, apparent ileal phosphorus digestibility (AIDP), and bone mineralization in broiler chickens. PHY1 and PHY3 resulted in better growth performance compared to PHY2 and PHY4, and performed equally to the positive control diet. All phytase treatments resulted in similar effects in terms of bone mineralization and AIDP.
The document discusses achieving inorganic phosphorus-free broiler production through optimized phytase and diet formulations. It outlines several approaches: using a highly efficient phytase to breakdown most phytate, stimulating gizzard development, sufficient phytate phosphorus levels from plant ingredients, optimized calcium levels based on limestone solubility, and phase-specific phytase dosing strategies. Trials with Ross 308 broilers were set up with varying phytate phosphorus levels in diets containing corn, wheat, soybean, rice, wheat bran, and rapeseed/sunflower meals. Phytase and xylanase were included at specified levels while meeting minimum metabolizable energy and nutrient specifications compared to breeder recommendations.
This research evaluated the effect of a novel protease by DSM (RONOZYME® ProAct) on the performance, digestible energy and the ileal digestibility of crude protein, fat, gross energy and amino acids in broiler chickens. Read more to learn about the results.
Visit us at DSM Animal Nutrition and Health to learn more about our business: http://www.dsm.com/markets/anh/en_US/home.html
The document discusses optimizing protein digestion in poultry through the use of exogenous proteases, noting that both endogenous proteins secreted by the bird and undigested dietary proteins leave the ileum undigested. It examines factors that influence protein digestion like diet composition and quality, and endogenous protein sources and recovery. Models are presented that can help predict how diet inputs like crude protein level and amino acid ratios influence the efficacy of adding an exogenous protease supplement to improve protein digestion and bird performance.
This document discusses key factors to consider when selecting feed enzyme products for poultry production. It outlines three main classes of enzymes used in poultry feeds - phytases, carbohydrases, and proteases. When choosing an enzyme product, it is important to evaluate whether the enzyme will improve digestibility of the specific feed formulation, is thermostable for pelleting conditions, and is compatible with other enzymes. The document also stresses selecting enzymes that have proven mechanisms of action and are produced under quality manufacturing standards.
Knowledge of amino acid (AA) digestibility of feed ingredients is necessary to feed broilers with
properly balanced compound diets. For this reason, more attention has recently been given to the
determination of AA digestibility of ingredients, recognizing that it may vary greatly depending
upon the feed ingredient. The effects of the inclusion of a mono-component serine protease
(Ronozyme® ProAct) on standardized ileal amino acid digestibility (SIAAD) of diets containing wheat
by-products (wheat DDGS (WDDGS) and wheat middlings (WM)) were evaluated in broilers.
- Improvement of SIAAD in the presence of protease is not the same for all AA and varies from one ingredient to another one. The effects of added protease are dependent on feed composition and on intrinsic digestibility of AA (COWIESON and ROOS (2014)).
- Factors such as type and quality of the ingredients, industrial processing and the presence of anti-nutritional substances such as tannins, phytates, trypsin inhibitors in plant species modulate the digestibility in poultry feed and thus the effectiveness of exogenous protease.
-The physiological state of animals (growth or maintenance), feed consumption or the nutritional feed quality could also influence the digestibility values.
Animal feed accounts for 60-70% of livestock and poultry production costs. Supplementing feed with enzymes can help producers save costs by enabling animals to produce more meat faster and cheaper. There are several types of enzymes that play important roles in animal feed digestion. Proteases break down proteins, carbohydrases improve carbohydrate digestibility, and phytases allow animals to access phosphorus stored in plant feeds. Using enzymes can boost feed efficiency, lower feed costs, and reduce environmental impacts.
Enzyme technology is an integral tool for the brewing, baking and textile industries. In agriculture there is great potential for enzymes too but this is yet to be fully exploited. However, animal nutrition is one area in which the use of feed enzymes is becoming increasingly important.
This document discusses the use of exogenous enzymes in poultry nutrition as an alternative to antibiotics. It provides background on how antibiotics were commonly used but are now banned. Exogenous enzymes are one potential alternative as they can help break down non-starch polysaccharides in feed ingredients like wheat and barley to improve digestibility. The document outlines different types of enzymes and their sources and effects. It summarizes studies looking at how exogenous enzymes can reduce the size of digestive organs and positively impact gut morphology.
Phytate is a natural dietary content and constitutes 0.4–6.4% (w/w) of most cereals and legumes (Eeckhout and Deaepe, 1994). It is poorly digestible for monogastric animals due to
a lack of effective endogenous phytase (Bitar and Reinhold, 1972). Phytate acts as an antinutritional factor, exerting its effects via a reduction in the solubility, and availability of
phosphorus (P), and to a lesser extent, Ca, Zn, Fe (Nävert et al., 1985; Hallberg et al., 1987; Hurrell et al., 2003). It was also reported that phytate could decrease the utilization of protein,
amino acids and starch. It has been suggested that phytate may bind with starch either directly, via hydrogen bonds, or indirectly, via proteins associated with starch (Thompson,
1988; Rickard and Thompson, 1997). Phytate is also known to inhibit a number of digestive enzymes such as pepsin, alpha-amylase (Deshpande and Cheryan, 1984) and increase mucin
secretion, excretion of endogenous minerals and amino acids in broiler chickens (Liu et al., 2008). Another issue is higher cost of dietary inorganic P which has been increased remarkably in last decade because of shortened phospate sources. Poultry industry has still been growing and reached huge mass production and contribution to environmental pollution has been heightened concerns because of the poor utilization of phytate phosphorus by poultry.
This document summarizes research on the use of organic acids in animal nutrition. It discusses how organic acids have gained importance in feed industry following the ban of antibiotic growth promoters. Short chain organic acids can have antimicrobial properties. Research shows that organic acids can improve animal performance by reducing pathogenic bacteria counts, increasing nutrient digestibility, and improving gut health. Studies demonstrate that supplementing animal feed with organic acids like formic acid, fumaric acid, and butyric acid can positively impact growth performance, gut morphology, and nutrient utilization in poultry and pigs.
Phytase is an enzyme that helps non-ruminant animals like poultry and swine break down and absorb phosphorus from grains. It allows these animals to utilize phosphorus from phytate in grains, reducing the need for supplemental phosphorus in their diets. Phytase is produced by microbes like bacteria and fungi. It works by hydrolyzing phytate, making phosphorus more available to the animal. The use of phytase supplements in animal feed lowers feed costs and decreases phosphorus pollution by allowing animals to excrete less phosphorus.
This chapter provides an overview of the current feed enzyme market and expected future developments. The global feed enzyme market has grown substantially since the late 1980s as enzymes help improve feed efficiency and flexibility in feed formulation. Enzymes allow animals to extract more nutrients from feed by targeting anti-nutritional factors. While animals naturally produce some digestive enzymes, they cannot fully digest 15-25% of feed due to indigestible components. Feed enzymes help bridge this gap to improve animal performance and farm profitability by lowering feed costs. The market is expected to continue expanding with the use of enzyme combinations and customization to specific feed formulations.
A description and the results of research carried out on broiler chickens in order to explore the efficacy of phytase products on ileal digestibility of phosphorus.
The reseach results found that phytase supplementation was effective in improving the growth performance, ileal phosphorus digestibility and the bone mineralization parameters when included in the low phosphorus diet.
Visit us at: http://www.dsm.com/markets/anh/en_US/home.html
This study investigates the effect of Bacillus licheniformis protease on the growth performance and the nutrient utilization of broilers. This reduces dietary requirements and environmental impact of excretion whilst increasing energy utilisation and growth in broilers.
Join the LinkedIn discussion on Feed Enzymes:
http://www.linkedin.com/groups?home=&gid=4738175&trk=anet_ug_hm&goback=%25
Learn more about DSM Animal Nutrition at: http://www.dsm.com/markets/anh/en_US/home.html
Or why not follow us on Twitter: @DSMFeedTweet
Research has demonstrated that phytase is the only enzyme that is able to initiate
the release of phosphorus (P) from the phytate molecule, making it available for
absorption and utilization (Selle and Ravindran, 2007). The industrial demand for
phytases with greater potency in intestinal phytate hydrolysis and better heat
stability continues to stimulate the search for new enzyme sources. Enzyme
preparations with phytases derived from A. ficuum, Peniophora lycii and E. coli are
available commercially. More recently, new microbial 6-phytases produced by
synthetic genes, mimicking a gene from C. braakii or isolated from Buttiauxella,
were introduced into the market.
Protein/amino acids are among the most expensive nutrients to deliver in poultry nutrition
The digestibility of protein in poultry is typically incomplete by the terminal ileum
Undigested protein that leaves the ileum is from both exogenous (diet) and endogenous (bird) sources
Understanding the digestion of dietary proteins and the recovery of endogenous proteins is important and can provide a basis for the use of exogenous proteases
Presentation on Non starch polysaccharides in poultry final osrHarshit Saxena
Include knowledge of NSPs n important constituent in modern poultry farming . Mitigation and their utilization in non-conventional feeds remarkably increases profit
This study compared the efficacy of four phytase products - PHY1 from Citrobacter braakii, PHY2 from E.coli, PHY3 from E.coli, and PHY4 from Peniophora lycii - on growth performance, apparent ileal phosphorus digestibility (AIDP), and bone mineralization in broiler chickens. PHY1 and PHY3 resulted in better growth performance compared to PHY2 and PHY4, and performed equally to the positive control diet. All phytase treatments resulted in similar effects in terms of bone mineralization and AIDP.
The document discusses achieving inorganic phosphorus-free broiler production through optimized phytase and diet formulations. It outlines several approaches: using a highly efficient phytase to breakdown most phytate, stimulating gizzard development, sufficient phytate phosphorus levels from plant ingredients, optimized calcium levels based on limestone solubility, and phase-specific phytase dosing strategies. Trials with Ross 308 broilers were set up with varying phytate phosphorus levels in diets containing corn, wheat, soybean, rice, wheat bran, and rapeseed/sunflower meals. Phytase and xylanase were included at specified levels while meeting minimum metabolizable energy and nutrient specifications compared to breeder recommendations.
RONOZYME® HiPhos phytase is a DSM feed enzyme used to improve animal nutrition and health. The presentation will describe the effects of phytase on a number of elements, as well as the positive results, which effectively improved several response variables in turkey poults.
Visit us at: http://www.dsm.com/markets/anh/en_US/home.html
API for Feed Mill.Those are manufacturer they need this rate file.Please contact with me for details.Md.Enamul haque.Mobile:008801673609620 (viber and whatsapp)Email-enamulctg@yahoo.com and sumonpbd@gmail.com
NIZO Plant Protein Functionality Conference on October 21-22 gathered around 450 attendees to discuss the recent findings and innovations on plant proteins. Research team leader Emilia Nordlund gave a keynote presentation on bioprocessing technologies to improve the plant protein functionality.
'Lo último en obesidad'. Este es el título del Simposio Internacional que organizamos en la Fundación Ramón Areces los días 1 y 2 de diciembre de 2015. En colaboración con la Fundación General CSIC, reunió a algunos de los mayores expertos en la materia para analizar cómo reducir este grave problema de salud pública.
Global farmed crustacean production has been increasing at much faster rate than major farmed species. It is expected to reach 6.8 million metric tons (MMT) in 2015, of which, 69 percent or 4.7 MMT will be from the marine shrimps (Figures 1A, 1B). In the last 15 years, the production increased six-fold driven more by intensification rather than expansion of the area cultivated.
Asrt malaysia itm rice bran projects presentationAmr Helal
Rice bran , instead of using as animal feed, was stablized , studied from the phytochemical , pharmacological, toxicological and formulation points of view. It is developed to be used in bakery, nutraceutical, cosmoceutical preparations with soild scientific proof of evidence.
Can proteases play a role in enteric health- Langhout, P. Presentation for Workshop 4, at the Feed Proteases and enzyme presentation, The Netherlands, 2014
This study evaluated the effects of graded levels of the dietary probiotic Hydroyeast Aquaculture on growth performance of adult Nile tilapia (Oreochromis niloticus). Fish were fed diets containing 0, 5, 10 or 15 g of probiotic per kg of feed. Results showed that supplemental probiotic significantly improved final weight, weight gain, daily gain, and growth rates in both male and female fish in a dose-dependent manner compared to the control group. The highest growth performance was observed in fish fed 10 g or 15 g of probiotic per kg of diet. The study suggests that dietary probiotic can be used to enhance growth of adult tilapia.
In recent years, aquaculture has gained in importance as a renewable source of dietary protein and as a viable commercial activity. To maintain this position in the future and to continue to provide a good investment opportunity, the problems the sector currently faces must be addressed. One of the more important of these concerns is the cost of feed, which is estimated to be 50-60 percent of the total cost of production. Numerous studies on the use of different feed formulations, feed ingredients and feeding techniques have been conducted (Kaushik et al., 2004; Thiessen et al., 2003; Martinez et al., 2004; Enes et al.,2006; Izquierdo et al., 2003). These studies have included assessments of various alternative raw materials, vitamins and minerals, monitoring the amount of feed provided to the fish, and the addition of pigments and other feed additives to the diet. In particular, various feed additives with growth promoting properties came into prominence in these studies (Francis et al., 2005; Haroun et al., 2006; Abdel-Tawwab et al., 2008; Lara-Flores et al., 2003; Li and Gatlin, 2004;). Growth promoting feed additives may contain different ingredients as plant extracts, organic acids, probiotics, hormones etc.
Liver Histological Response of Hyperlipidemic Male Rat (Rattus norvegicus) to...AI Publications
The leaf of lakum (Cayratia trifolia (L.) Domin.) contains compounds that are expectedly antihyperlipidemic. This study aims to determine the liver histological response to the lakum leaf extract and its effect on the levels of SGOT and SGPT in hyperlipidemic male rats (Rattus norvegicus). The male Wistar rats were randomly divided into 4 treatment groups. P0 was a group of rats that were given standard feed, P1 was a group that were fed with high fat diet, P2 was a group that were fed high fat diet and lakum leaf extract with a dose of 40mg/200g BW (body weight)/day, and P3 was a group were fed high fat diet and simvastatin at a dose of 0.18mg/200g BW/day. High fat diet was given for 30 days, whilst treatments were implemented for 28 days. The histological descriptions showed that the hepatocyte cell repair occurred in the treatment group that was given lakum leaf extract but there were no significant differences in liver weight as well as SGOT and SGPT levels. Lakum leaf extract can be used as an antihyperlipidemic agent whilst maintaining the histology of hepatocytes and would not interfere with the liver function of the hyperlipidemic male rats.
Partial purification and characterization of extracellular protease from pedi...Mushafau Adebayo Oke
This document summarizes a study that characterized and partially purified an extracellular protease produced by Pediococcus acidilactici. Key findings include:
- The protease showed optimal activity at a casein concentration of 2% and with 2.5 ml of crude enzyme.
- It had temperature and pH optima of 28°C and 4.0, respectively, indicating it is a mesophilic and acidic protease.
- Purification using gel filtration chromatography resulted in a 2.26-fold increase in purification and an estimated molecular weight between 45-66 kDa via SDS-PAGE.
Factors affecting storage stability of various commercial phytase sourcesThanh Doan
This study evaluated the effects of storage conditions on the stability of six commercial phytase products over 360 days. Phytases were stored either in pure form, mixed with a vitamin premix, or mixed with a vitamin and trace mineral premix at temperatures of -18°C, 5°C, 23°C, or 37°C. Storage stability was reported as residual phytase activity compared to initial levels. Results showed that phytase stability was affected by storage duration, temperature, product form, coating, and phytase source. Pure products stored at 23°C or below retained the highest levels of activity. Coating helped mitigate losses at higher temperatures. Mixing phytases with premixes and
Effects of Cinnamon Powder on Egg Quality: A New Approach using Layer BirdsAI Publications
Attention towards natural growth promoters in poultry has been on the increase in different parts of the world. However, most studies focus on broiler birds and quail. This study analyzed how cinnamon powder supplemented in the diet of Lohmann Brown layer birds can improve egg quality. This study consisted of 5 treatments of 30 birds per treatment, comprising fifteen replicates of 2 birds per replicates. The treatments included: T1: Control without Cinnamon; T2: 4 litres of water + 0.1g of cinnamon; T3: 4 litres of water + 0.2g of cinnamon; T4: 4 litres of water + 0.3g of cinnamon; T5: 4 litres of water + 0.4g of cinnamon. Parameters measured were egg weight, egg mass, egg breadth, egg length, albumen height, albumen weight, yolk height, yolk length, yolk colour, yolk weight, shell weight, and shell thickness. The results revealed an increase in the albumen weight, yolk weight, albumen height, and yolk height as the inclusion levels of cinnamon powder increase. In addition, the egg weight was highest in treatment 5. Taken together, cinnamon powder addition in the diet of Lohmann Brown layer birds has a positive outcome on egg quality.
1) Salmon ovary peptide is generated from the outer coat of salmon ovary through an enzyme decomposition process. It is expected to have anti-aging effects and be used in cosmetics and foods.
2) Testing showed salmon ovary peptide has antioxidant effects by controlling lipid peroxide production and inhibiting tyrosinase activity. It also displayed SOD-like antioxidant activity.
3) In studies with rats, salmon ovary peptide was found to protect liver function, control blood sugar levels, and regulate fat accumulation in visceral adipocytes by inhibiting their maturation and bloating.
ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE EFFECTOF POMEGRANATE PEEL AND WHEY...Anurag Raghuvanshi
The antioxidant activity of pomegranate peel powder (PPP) and whey powder (WP) was evaluated, their hepatoprotective effect of each alone or in combination (PPWP) at equal levels was also evaluated in Wistar rats against carbon tetrachloride (CCL4) induced liver injury.
The hepatoprotective activity was assessed using various biochemical parameters and histopathological studies.
3. Antibiotic Concerns Prompt a Different Approach by Dr. Sirish NigamInayath Ulla Khan
Antibiotic concerns have prompted a different approach to animal feeding in Europe. The use of antibiotics as growth promoters was banned in the EU in 2006 due to concerns about antibiotic resistance. This has led to increased focus on non-antibiotic alternatives like improving feed quality, adding enzymes, probiotics, prebiotics, organic acids, and secondary plant compounds. Feeding fermentable fibers through processes like LTCL that modify starch and structural carbohydrates can support gut health and performance in broilers without the use of antibiotics.
3. Antibiotic Concerns Prompt a Different Approach by Dr. Sirish Nigam
Leveking Phytase Introduction
1. Optimized Usage of Phytase
in Animal Nutrition
- Going beyond phosphorus release
- Much more release of energy
2. 1
2
Content
Introduction of Leveking (p2-p7)
Introduction of Phytate (p8-p12)
Introduction of Leveking Phytase (p13-p19)
Experiment and Conclusion (p20-p44)
3. Shenzhen Leveking bio-engineering Co. Ltd. is a National High-tech enterprise,
integrating enzyme R&D, production, application and marketing. Our products
and solutions apply in food、animal nutrition, leather tech, paper-making and so
on. Leveking Animal Nutrition is a subsidiary of Leveking, focusing on enzyme
development and application in animal nutrition.
As a leading feed lipase enterprise in China, Leveking owns a strong property
rights system and obtains a number of national invention patents.
Introduction of Leveking
4. 4 Enzyme Production Bases, ensure high quality and effective supply.
Production Base in Shenzhen
Pilot plant for high-end enzyme production.
Production Base in Ningxia
A large production base in Ningxia, North China.
Production Base in Anhui
Main production base with latest fermentation
equipment and production technology.
Production Base in Guangming
For food and feed enzyme compounding
with clean workshop of hundred thousand level.
Production Bases
8. 1
2
Content
Introduction of Leveking (p2-p7)
Introduction of Phytate (p8-p12)
Introduction of Leveking Phytase (p13-p19)
Experiment and Conclusion (p20-p44)
9. Anti-nutrient effect of phytate
2 3 4 5
Binds with protein
(Wise 1983)
Chelates with calcium and trace-elements
(Wise 1983)
Acidic pH Basic pH
1
32
56
OH
P OOH
O
H
OH
P OOH
O
H
H
OH
P OOH
O
H
O H
PO O H
O
OH
P OOH
O
H
H
O
H
P OOH
O
4
Phytate reduces the availability of protein,
calcium, trace-elements and possibly other
nutrients such as starch
pH 4.5
10. Disadvantage of Phytate
Phytate in the feed can prevent phosphorus absorption for monogastric
animals, which increases feed cost;
Phytate chelates with minerals in the feed so that them can not be
utilized by the animal and more inorganic minerals shall be supplemented;
Phytate will combine with amylase, protease and lipase in the feed, to
inhibit activity of the endogenous enzymes, leading to a indigestibility of
proteins and amino acids;
Phosphorus excreted to the environment leads to pollution.
12. Strong correlation between phytate breakdown
and protein digestibility in vivo
Phytate Breakdown VS Digestibility
R²= 0.67
60
65
70
75
80
85
90
95
20 30 40 50 60 70 80 90 100
TotalAAdigestibility(%)
Phytate degradation (%)
Amerah et al., 2012
13. 1
2
Content
Introduction of Leveking (p2-p7)
Introduction of Phytate (p8-p12)
Introduction of Leveking Phytase (p13-p19)
Experiment and Conclusion (p20-p44)
14. Benefits of Phytase
Improve phosphorus utilization in the feed and reduce addition of calcium
hydrogen phosphate so as to lower feed cost.
Relieve the chelation of the phytate with digestive enzymes, maximize effect of
endogenous enzymes to enhance utilization of the nutrients.
Release mineral ions like Ca, Mg, Fe and Zn and improve utilization.
Release proteins and starch which were chelated with phytate and improve
their utilization.
Reduce phosphorus excretion and improve breeding environment and reduce
pollution.
15. 1. Leveking Phytase Overview
(1) The thermostable phytase developed by Leveking has been improved by
several times of strain improvement with genetic engineering technology. It has
increased greatly the utilization of phytase and reduced activity loss in pelleting
process.
(2) Phytase Activity Definition(GB/T18634-2009)
One unit of phytase activity, refers to the amount of phytase liberating 1μmol of
inorganic phosphate in 1 minute from sodium phytate (5.0mmol/L) at pH 5,5 and
37°C.
(3) Activity:
Granule: 5000 IU/g,10000 IU/g
18. 4. Specification
Form: pellet
Package: 25 kg/bag, paper-plastic lamination.
Storage: Shelf life of 12 months when stored at a cool, dry and ventilated
place and avoid high temperature.
Activity (IU/g) 5000 10000
Dosage for Broiler(g/T) 100 50
Dosage for Laying
hen(g/T)
80-100 40-50
19. 1
2
Content
Introduction of Leveking (p2-p7)
Introduction of Phytate (p8-p12)
Introduction of Leveking Phytase (p13-p19)
Experiment and Conclusion (p20-p44)
20. Design:4800 broilers of 1 day age were divided into 6 groups, 4 repeat
and 200 each repeat.
Group Diet
1 Positive Control(PC)
2 Negative Control (NC reduced by 0.13% Avail. P, 0.143% Ca, 0.03% Na)
3 NC+250 IU/kg phytase
4 NC+500 IU/kg phytase
5 NC+1000 IU/kg phytase
6 NC+2000 IU/kg phytase
Period:84 days.
Experiment I. Effect on Growth Performance of AA Broilers
21. Group Final Weight(g) Feed Intake(g) FCR (%)
Gender Male Female Male Female Male Female
PC 9204bc 6491bc 18292ab 13682bc 1.988bc 2.109ab
NC 8662d 6280d 17954ab 13498c 2.073a 2.150a
NC+250 IU/kg 8833d 6409c 17896b 13624c 2.026ab 2.126a
NC+500 IU/kg 9141c 6588b 17942b 13693bc 1.964c 2.079b
NC+1000 IU/kg 9375ab 6734a 18417ab 13932ab 1.965c 2.069b
NC+2000 IU/kg 9505a 6809a 18727a 14127a 1.970bc 2.075b
Experiment Results on Growth Performance of AA Broilers
22. 0
2500
5000
7500
10000
12500
PC NC 250U/kg 500U/kg 1000U/kg 2000U/kg
DailyGain(g)
Effect of Phytase on Daily Gain
Male
Female
250 IU/kg
Experiment Results on Growth Performance of AA Broilers
500 IU/kg 1000 IU/kg 2000 IU/kg
23. 10000
12000
14000
16000
18000
20000
PC NC 250U/kg 500U/kg 1000U/kg 2000U/kg
DailyIntake(g)
Effect of Phytase on Daily Intake
Male
Female
Experiment Results on Growth Performance of AA Broilers
250 IU/kg 500 IU/kg 1000 IU/kg 2000 IU/kg
24. 1.80
1.90
2.00
2.10
2.20
2.30
PC NC 250U/kg 500U/kg 1000U/kg 2000U/kg
FCR(%)
Effect of Phytase on FCR
Male
Female
Experiment Results on Growth Performance of AA Broilers
2000 IU/kg250 IU/kg 500 IU/kg 1000 IU/kg
25. Experiment II. Effect on Growth Performance and
Serum Biochemical Indicators of Broilers.
Group Diet
1
Positive Control (PC, 0-3 weeks with TP 0.69%,0.45%
NPP; 3-6 weeks with TP 0.59%,0.35% NPP)
2
Negative Control (NC, 0-3 weeks with TP 0.60%,0.35%
NPP; 3-6 weeks with TP 0.49%,0.25% NPP)
3 NC+500 IU/kg phytase
4 NC+2000 IU/kg phytase
5 NC+8,000 IU//kg phytase
6 NC+32,000 IU/kg phytase
Design: 576 male AA broilers of 1 day age were divided into 6
groups, 8 repeat for each group, 12 broilers each repeat. Two periods: 0-
3 weeks and 3-6 weeks.
Grouping:
(Published in No.4, Vol38, 2011, Animal Science and Veterinary Medicine In China )
26. Composition
1-21 day age 22-42 day age
PC LP Diet PC LP Diet
Corn 57.08 57.54 59.47 59.92
Soybean oil 0.83 0.69 2.07 1.92
Soybean meal 37.01 36.92 34.84 34.75
Fish meal (Peru) 1.00 1.00 --- ---
Methionine 0.29 0.29 0.14 0.14
Limestone 1.01 1.41 1.13 1.54
DCP 1.98 1.35 1.5 0.93
Salt 0.30 0.30 0.30 0.30
Premix 0.50 0.50 0.50 0.50
Choline chloride --- --- 0.1 0.1
Diet Composition
Table 1. Diet Composition
27. Nutrient
1-21 day age 22-42 day age
PC LP Diet PC LP Diet
ME(MJ/kg) 12.14 12.14 12.26 12.26
Crude protein(%) 21.50 21.50 20.00 20.00
Crude fat(%) 3.33 3.20 4.51 4.38
Ca(%) 1.00 1.00 0.90 0.90
TP(%) 0.69 0.60 0.59 0.49
NPP(%) 0.45 0.35 0.35 0.25
Ca : P 1.45 1.67 1.53 1.84
Diet Nutrition
Table 2. Diet Nutrition
28. Initial Weight
(g)
Final Weight
(g)
Average Daily
Intake(g)
Average Daily
Gain(g)
FCR (%)
PC 38.69± 0.34a 761.05± 22.42ab 45.06±1.34a 34.31±1.07ab 1.31±0.050a
NC 38.70± 0.45a 743.09±24.32b 43.90±1.26a 33.45±1.16b 1.32±0.042a
NC+500 IU/kg 38.15± 0.79a 762.90±41.46ab 45.06±2.09a 34.39±1.98ab 1.31±0.084a
NC+2000 IU/kg 38.42± 0.24a 775.59±13.78ab 45.20±2.58a 34.40±0.66a 1.29±0.045a
NC+8000 IU/kg 38.05± 0.60a 777.58±45.36ab 45.03±2.11a 35.09±2.16a 1.28±0.072a
NC+32000 IU/kg 38.23± 0.83a 785.03±31.33a 45.39±0.89a 35.45±1.49a 1.28±0.050a
Table 3. Effect on Growth Performance of Broilers of 1-21 day age
Experiment Results
29. Initial Weight
(g)
Final Weight
(g)
Average Daily
Intake(g)
Average Daily
Gain(g)
FCR (%)
PC 761.05± 22.42ab 2095.63±105.82
c 113.80±5.56a 47.78±5.26c 2.33±0.20a
NC 743.09± 24.32b 2061.44±82.55c 110.73±4.72a 46.60±3.05c 2.45±0.18a
NC+500 IU/kg 762.90± 41.46a
b 2098.31±78.19c 111.92±5.44a 48.09±2.18c 2.33±0.20a
NC+2000 IU/kg 775.59± 13.78a
b 2192.75±84.74b 111.05±4.30a 52.29±4.57b 2.14±0.18b
NC+8000 IU/kg 777.58± 45.36
ab
2332.94±102.41
a 111.19±6.85a 57.48±4.24a 1.94±0.07c
NC+32000 IU/kg 785.03±31.33a 2344.88±81.21a 114.32±2.90a 56.90±2.90a 2.01±0.13b
Table 4. Effect on the Growth Performance of Broilers of 22-42 day age
Experiment Results
30. 113.8 110.73 111.92 111.05 111.19 114.32
40.00
60.00
80.00
100.00
120.00
140.00
PC NC +500 +2000 +8000 +32000
DailyIntake(g/d)
Graph - Table 4
Effect on Daily Intake of Broilers of 22-42 day age
Experiment Results
31. 47.78 46.60 48.09
52.29
57.48 56.90
20.00
30.00
40.00
50.00
60.00
70.00
PC NC +500 +2000 +8000 +32000
DailyGain(g/d)
Graph - Table 4
Effect on Daily Gain of Broilers of 22-42 day age
Experiment Results
33. Group Ca(mmol/L) P(mmol/L)
Total Protein
(g/L)
SAP(U/mL)
PC 2.89± 0.41ab 3.36±0.52a 27.02±5.40a 3.59±0.66c
NC 2.88± 0.22b 2.72±0.43c 25.78±3.01a 5.55±1.19a
NC+500 IU/kg 3.13±0.40a 3.29±0.31ab 27.03±2.51a 4.87±1.42ab
NC+2000 IU/kg 2.98±0.31ab 3.17±0.45ab 27.15±2.22a 4.60±1.66abc
NC+8000 IU/kg
2.86±0.31b 3.12±0.33ab 27.30±2.10a 3.85±1.35c
NC+32000 IU/kg 2.76±0.30b 3.06±0.37ab 27.53±2.01a 3.56±0.96c
Table 5. Effect of Phytase on the SAP( serum alkaline phosphatase) Activity, Total
Protein, Calcium and Phosphorus Levels of 21-day age Broilers
Experiment Results
34. Ca
(mmol/L)
Phosphorus
(mmol/L)
Total Protein
(g/L)
Alkaline
phosphatase
(U/mL)
PC 2.61± 0.20a 2.03±0.18bc 31.61±4.97ab 1.45±0.41b
NC 2.60±0.35a 2.00±0.32c 31.34±6.78ab 1.87±0.34a
NC+500 IU/kg 2.60±0.13a 2.11±0.22abc 33.29±6.32ab 1.68±0.73ab
NC+2000 IU/kg 2.57±0.18b 2.16±0.22ab 33.89±6.96ab 1.46±0.38b
NC+8000 IU/kg 2.57±0.21b 2.20±0.25a 34.90±5.41ab 1.43±0.33b
NC+32000 IU/kg 2.51±0.19b 2.19±0.19a 35.08±6.60a 1.45±0.23b
Table 6. Effect of Phytase on the SAP (serum alkaline phosphatase) Activity, Total
Protein, Calcium and Phosphorus Levels of 42-day age Broilers
Experiment Results
35. 2.61 2.60 2.60 2.57 2.57 2.51
1.50
1.80
2.10
2.40
2.70
3.00
PC NC +500 +2000 +8000 +32000
Ca(mmOl/L)
Graph – Table 6
Effect on Serum Ca Level of 42-day age Broilers
Experiment Results
36. 2.03 2.00
2.11 2.16 2.20 2.19
0.50
1.00
1.50
2.00
2.50
3.00
PC NC +500 +2000 +8000 +32000
P(mmol/L)
Graph – Table 6
Effect on Serum Phosphorus Level of 42-day age Broilers
Experiment Results
37. Conclusion
Supplement of phytase from 500 IU/kg increased to 2000 IU/kg can
improve daily gain and FCR further.
According to plenty of experiment data, 2000 IU/kg of Leveking
phytase can release 0.15% of available P. and 40-50kcal/kg of energy.
The accurate remaining activity of phytase can be aasayed by
pelleting recovery rate, so as to help nutritionist optimize the usage
of phytase.