The document discusses laboratory tests used to identify enteric bacteria, including the IMViC test, TSIA test, and Sims test. The IMViC test checks for indole production, methyl red reaction, Voges-Proskauer reaction, and citrate utilization. The TSIA test checks for fermentation patterns and hydrogen sulfide production. The Sims test checks for motility, indole production, and hydrogen sulfide production. Control organisms used include E. coli, Enterobacter aerogenes, and Citrobacter freundii.
Each of the letters in “IMViC” stands for one of these tests. “I” is for indole; “M” is for methyl red; “V” is for Voges-Proskauer, and “C” is for citrate, lowercase “i” is added for the ease of pronunciation. IMViC is an acronym that stands for four different tests
Indole test
Methyl red test
Voges-Proskauer test
Citrate utilization test
Each of the letters in “IMViC” stands for one of these tests. “I” is for indole; “M” is for methyl red; “V” is for Voges-Proskauer, and “C” is for citrate, lowercase “i” is added for the ease of pronunciation. IMViC is an acronym that stands for four different tests
Indole test
Methyl red test
Voges-Proskauer test
Citrate utilization test
A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
INDOLE TEST
UREASE TEST
CITRATE TEST
METHYL RED(MR) TEST
VOGES – PROSKAUER(VP) TEST
TRIPLE SUGAR IRON(TSI) TEST
OXIDASE TEST
CATALASE TEST
CATALASE TEST-Principle:-
This test demonstrates presence of catalase enzyme. This enzyme catalyses the release of O2 from H2O2.
catalase
2H2O2 H2O + increase O2
Reagents:- 1) 3% H2O2.
2) 24 hrs cultured organisms
Procedure:-
With sterile wooden stick transfer culture organisms to test tube containing 3% H2O2 and observe for production of effervescence.
It can also be tested directly on growth plate.
Positive Control: Staphylococci.
Negative Control: Streptococci.
False positive reactions:
If culture medium contains catalase enzyme e.g., blood agar, chocolate agar.
If iron wire loop is used
Biochemical tests are based on reactions that takes place in various living rganisms. In microbiology these are useful for identification of various microorganisms like identification and differentiation of various bacterial species. IMViC test is a group of test that are used to differentiate between Escheritia and Enterobacter species.
A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
INDOLE TEST
UREASE TEST
CITRATE TEST
METHYL RED(MR) TEST
VOGES – PROSKAUER(VP) TEST
TRIPLE SUGAR IRON(TSI) TEST
OXIDASE TEST
CATALASE TEST
CATALASE TEST-Principle:-
This test demonstrates presence of catalase enzyme. This enzyme catalyses the release of O2 from H2O2.
catalase
2H2O2 H2O + increase O2
Reagents:- 1) 3% H2O2.
2) 24 hrs cultured organisms
Procedure:-
With sterile wooden stick transfer culture organisms to test tube containing 3% H2O2 and observe for production of effervescence.
It can also be tested directly on growth plate.
Positive Control: Staphylococci.
Negative Control: Streptococci.
False positive reactions:
If culture medium contains catalase enzyme e.g., blood agar, chocolate agar.
If iron wire loop is used
Biochemical tests are based on reactions that takes place in various living rganisms. In microbiology these are useful for identification of various microorganisms like identification and differentiation of various bacterial species. IMViC test is a group of test that are used to differentiate between Escheritia and Enterobacter species.
ISOLATION AND IDENTIFICATION OF NLF BACTERIA IN VARIOUS SAMPLES.Daisy Saini
IDENTIFICATION AND ISOLATION OF NON-LACTOSE FEREMNTING BACTERIA IN VARIOUS CLINICAL SAMPLES IN A TERTIARY HOSPITAL IN INDIA, INCLUDE BIOCHEMICAL TEST BASE ON THEIR ENZYMATIC ACTIVITY AND GRAPHICAL PRESENTAION OF THEIR DISTRIBUTION ACCORDING TO SEX RATION , AGE GROUP, SAMPLE AND THEIR PROFILE.
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